| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,587,531 | G→A | L195F (CTC→TTC) | ugpQ ← | glycerophosphodiester phosphodiesterase UgpQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,587,531 | 0 | G | A | 98.7% | 261.5 / NA | 78 | L195F (CTC→TTC) | ugpQ | glycerophosphodiester phosphodiesterase UgpQ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (39/38); minor base C (1/0); total (40/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGATTTCG > NC_000913/3587293‑3587775 | gcaTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGAtt < 2:444782/251‑1 (MQ=255) gctgcCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATg < 2:143607/251‑1 (MQ=255) ctgcCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATg > 2:398098/1‑250 (MQ=255) gcCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAAt < 1:46790/251‑1 (MQ=255) ggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGc > 2:581503/1‑251 (MQ=255) ggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGc < 1:269951/251‑1 (MQ=255) ggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACGATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCTGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGc < 2:203074/251‑1 (MQ=255) aTGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAAATCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGAAGCCCAGCcgcgcg > 1:281160/1‑251 (MQ=255) cAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTc < 1:45349/250‑1 (MQ=255) cccAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCATCCCAGCCGCGCGGTCAGTTCgcgc > 1:25251/1‑251 (MQ=255) ccgcAGCATGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTACGGCGTCTTTCAACTGCATCACTAGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCAGCGCGGTCAGTTCGCGCCAGtcgtcg > 1:128335/1‑251 (MQ=255) aGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGcc > 2:581336/1‑251 (MQ=255) cATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATc > 1:212620/1‑251 (MQ=255) ccGTTGAAAACTCTTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACAAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGc > 1:415436/1‑251 (MQ=255) ccGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAGTCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAg < 1:582550/250‑1 (MQ=255) cGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGc > 2:144848/1‑250 (MQ=255) aaaaCTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACaaa < 2:142528/251‑1 (MQ=255) aaaCTATAGGGCCGTAAAGTGCGGCCCAATCCCGACAATAGCATCGGTACAGATGCAATCAACAACCCAGCGCAGCAACTATGATGCGAGATGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGCGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCCACAAAc > 2:69530/1‑251 (MQ=255) aaaCTAGTGGGCCGTAAAGGTCGGACCAATCACGTCAATCGCATCGGTACAGAGGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAAGCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGAGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGGGCGCGCCATTCGTCGCGCCACTCATCCAGCAACAAAc < 2:422542/251‑1 (MQ=255) aCTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACcg > 1:470023/1‑251 (MQ=255) aCTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAAATGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCCTCCAGCACCAAACcg > 2:347952/1‑251 (MQ=255) ccGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGt < 2:25251/251‑1 (MQ=255) tAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCAACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCCCTCATCCAGCAACAAACCGCGCGGCAGTTcc > 2:261042/1‑251 (MQ=255) aaaGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCg < 2:270315/251‑1 (MQ=255) ggACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACAACCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGAGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgccgc > 2:240886/1‑251 (MQ=255) aCCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCCTCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAACCCGCGCGGCAGCTCCGGTgccgccc > 2:158546/1‑250 (MQ=255) cAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCtgt < 1:581336/251‑1 (MQ=255) aCGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGc < 2:457066/251‑1 (MQ=255) gTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGc > 2:167881/1‑249 (MQ=255) aTCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTc > 1:225340/1‑251 (MQ=255) cGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGc < 1:265047/250‑1 (MQ=255) ggTACAGATGCAATCCACACCCCAGCGCAGCCACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCAt > 1:480333/1‑251 (MQ=255) ggTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCAt < 1:144848/251‑1 (MQ=255) gTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAAGCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATc < 2:460263/251‑1 (MQ=255) tGCAATCCACCCCCCAGCGCAGCAACTCTGATGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAAtctc > 1:178155/1‑250 (MQ=255) cacCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGAc > 2:121200/1‑251 (MQ=255) ccccAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGAcag < 1:229410/251‑1 (MQ=255) ccAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGAcagc > 1:473567/1‑250 (MQ=255) aGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACagcagc < 1:458346/251‑1 (MQ=255) gcgcAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGGTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAgcg < 2:520497/251‑1 (MQ=255) cgcAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAgcgg > 1:55851/1‑251 (MQ=255) agcaTCTCTGCTGCGCGCTGGGGTTTAGTGACGGTATAAACCAGAATCCGCAGTTCTGCGTCGTTCAACAGCATCACTCGCGCGGTGTGGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGGTCGCGCCAGTCGTCGCGCCGCTCATCCAGCAACAAACCGCGCGGCAGTGCCGGTGCCGCCTGTGGTGCAGCTTCTAAAGCATCAAGCTCAAACGATGTCAGCAgcggcgg < 1:231116/251‑1 (MQ=255) agcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAgcggcgg > 1:554461/1‑251 (MQ=255) aaCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGt > 1:50438/1‑251 (MQ=255) gctgcGCGCTGTGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCTCTTTATTGAATAACGTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCAt < 2:564225/248‑1 (MQ=255) cgcgcTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGcc < 2:321046/251‑1 (MQ=255) cgcgcTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCGCAAACTATGACAGCAGCGGCGGCGTCATACCGGcc < 2:244473/251‑1 (MQ=255) gcTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCcac > 2:181522/1‑251 (MQ=255) ggggTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCcaca < 2:288111/249‑1 (MQ=255) ggggTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGc > 1:62307/1‑251 (MQ=255) gTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGCTTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTcc > 2:426946/1‑250 (MQ=255) ttATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTcgcg > 1:105888/1‑251 (MQ=255) gACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTgcc < 2:62307/250‑1 (MQ=255) cGGTATAGACCAGAAGCCGCAGTCCGGCGGCTTGCAACGGCAGCAACCGCGCTTTATTGAATAACTGATGATGGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGGTCGCGCCAGTCGTCGCGCCGCTCATCCGGCAACAAACCGCGCGGCAGTTCCGGGGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCGCACTGCTCGCGTgccgcc < 2:73841/251‑1 (MQ=255) ggTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAAAGATGACAGCAGCGGCGGCGTCATACCGGCCCACACCTAGCGAgccgcc > 1:511311/1‑250 (MQ=255) tataAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAgc > 1:188757/1‑251 (MQ=255) ataAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCAGCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCGCGCGTGCCGCCAgc < 1:66327/250‑1 (MQ=255) aagccAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCATCCGCGCGGTCAGTTCGCGCAAGTCGTCGCGCCACTCATCCCGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCATCTAAAGCCTCAATCTCAAACGATGACAGCAGCGGCGGCGTCATAACGGCCCACAGCTAGCGAGCCGCCAGCGcc > 2:444611/4‑251 (MQ=255) aCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGccac < 2:310739/251‑1 (MQ=255) aGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGACTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCCGATTCTAAAGCATCAATCTCAACCGATGCCAGCAGCGGCGGCGTCATCCCGGCCCACAGCTCGCGTGCCGCCCGCCccaccct > 1:202260/1‑249 (MQ=255) aTCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCAtttt < 1:138398/251‑1 (MQ=255) ccGCAGTCCGGCGTCGTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTg < 2:178113/250‑1 (MQ=255) cGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGcc > 2:216957/1‑251 (MQ=255) cAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGCTTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACACACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCCGCGGCGGCGTCCTACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCg > 2:74367/1‑251 (MQ=255) ccGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTaa < 1:216957/251‑1 (MQ=255) ccGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGACGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCCCAGCTCGCGTGCCGCCAGCGCAACCATTTTGCCCGTTa > 1:69330/1‑250 (MQ=255) ggCGTCTTTCACCTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTAGCGCCAGTCGTCGCGCCACTCATCCAGGAACAAACCGCGCGGCAGTTCCGGTGACGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGCCAGAAGCGGCGGCGGCATACCGGCCCACAGCTCGCGTGCAGCCAGCGCCACCCTTTTGCCCGTTCATg > 2:73024/1‑251 (MQ=255) tttCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCCTTTTGCCCGTTAATGGCccgg > 2:139742/1‑251 (MQ=255) tCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCccggt < 1:398098/250‑1 (MQ=255) aCTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCATTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTggg < 2:388287/251‑1 (MQ=255) cgcgCTTTATTGAATAACTTATGATTGAGATGAATAGAGCCGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGGCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGt < 1:294585/249‑1 (MQ=255) gcgcTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCCGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTg < 2:470023/251‑1 (MQ=255) cgcTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGa < 1:347952/251‑1 (MQ=255) tttATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGAttt > 1:218157/1‑251 (MQ=255) ttATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGAAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGAtt < 1:35201/249‑1 (MQ=255) ttATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTAGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCAAGCGCCACCATTTTGCCCGTTAATGGCCCGGGGCCGGTGGTGGGTTTGAGTTc > 2:475310/1‑251 (MQ=255) tATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGATTTc < 1:451288/250‑1 (MQ=255) aTTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCCGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGATTTCg > 1:500515/1‑250 (MQ=255) | GCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGATTTCG > NC_000913/3587293‑3587775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |