| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 303,541 | G→A | R184K (AGA→AAA) | yagU → | DUF1440 domain‑containing inner membrane protein YagU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 303,541 | 0 | G | A | 94.7% | 46.8 / NA | 19 | R184K (AGA→AAA) | yagU | DUF1440 domain‑containing inner membrane protein YagU |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/9); minor base C (1/0); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCA > NC_000913/303301‑303764 | ttACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGGGCTTTAGCCCAACTTTTTTTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGCTCTCCCGTGGTATGAGGATGTTTCTGGAATTTTTTGACATTTAGTCTGGTTTTGGTCTATTGAAATTATTCGCAACGATTTAct > 1:91237/1‑249 (MQ=255) aCGCACATTAACTTCTGGAGAGTGTTTCATGTCGGTTATTGGGTGGACGCTGAAGTAGTTCCAAAAAATAACCTCTGGCCGGCCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGATCCTATGATTTCCTCACCTCTCATGGGGCGGACGCCACATCTGTTTGATCTCCTGGGGGATGAGCACGGTTCTGAAATTGTTGGACGTTTAATATGGGTCTGGACTTTGGAAATTATTCGCACAAATTTACGaa > 2:367812/1‑250 (MQ=255) gTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTCGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTg > 2:148687/1‑247 (MQ=255) cGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATcc < 1:557642/251‑1 (MQ=255) ggTTATCGTGTGGTCGCTGAAGTATTTACAAAAATTAAACTCTGGCAGGGCTTCCTGGCAGGCGCTTTTGCCCAAATTTTTGTTCATATTATTTCATTCCCTCTCATGGGACTGACGCCACCTCTCTTTGATCTCCCGTGGCATGAGAATGTTTCTGAAATTTTTTGCCATTTAGTCTGGTTCTGGTCTTTTGAAATTATTAGCAAAGATTTACGAAACAGAATTACTCATGAGCAAGACCATGAGATccc > 2:528774/1‑251 (MQ=255) ctGGCAGGGCTTAATGGCAGGTGCTTTAGCCCAACTTTTTGTTCCTATTATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGGATGAGAATGTTTCTGAAATTTTTGGACCTTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATTAGCCAGACCCTGCGCTCCCTTTAGGCTCAAACAGAAAATGCATTGACTGATAAAAATGgc > 2:48347/1‑251 (MQ=255) ggCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATTCATTGAATGATAAAAATGgcgc > 2:4250/1‑251 (MQ=255) gCAGGTGCTTGAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCAt < 1:356820/251‑1 (MQ=255) ttGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTg < 1:101638/250‑1 (MQ=255) tCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCttt < 1:275171/250‑1 (MQ=255) atGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTtata < 1:391507/251‑1 (MQ=255) tGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAACACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTatat > 1:497268/1‑251 (MQ=255) ctcCCGTGGTGTGAGAGTATTGCTGAATTTTTTGGACATTTGGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACTATGAGATCTCTTTCTGCTCAAACAGGTAATGCATTGAATGATAAAAATGGCGCAAATACAACGCCATTTTTATAGGATAAACACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCGCCTTCAg > 2:528127/1‑247 (MQ=255) ctcCCGTGGGATGCGAATGTTTCTGAACTTTTTTGACATTTAGTCTGGTTCTGGTCTATTGAAATTATACGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACTCTGAGATACTGTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACATCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTCCTTCAg < 1:528127/247‑1 (MQ=255) ttttGGATATTTAGTCTGGTTCTGGTCTATTGCAATTATTCGCAAAGCTTTACGAAACAGAATTACTCATAAGCCAGACCCTGAGATCCCTTTAGGCTAAAACAGATAATGCATTGAATGATAAAAATGCCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGATTTTTATATTCTGCTGCAGCTAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCTGAAGAACGCTACagaag > 2:332625/1‑249 (MQ=255) tAGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGGCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCAttgtt < 1:281710/250‑1 (MQ=255) aGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCAttgttg < 1:424494/250‑1 (MQ=255) aGTCTGGTTCTGGTCTATTGAAATTATTCGCAAAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAACACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGc < 2:497268/251‑1 (MQ=255) gttctggtCTATTGAAATTATTCGCAAAGATATACGAAACAGACTTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCa > 1:204335/1‑250 (MQ=255) | TTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCA > NC_000913/303301‑303764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |