Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,576,313	A→G	V250A (GTG→GCG)	gntU ←	low‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,576,313	0	A	G	100.0%	270.4 / NA	75	V250A (GTG→GCG)	gntU	low‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/39);  total (36/39)

CCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCACAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATC  >  NC_000913/3576074‑3576552                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ccAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGAAACCAGAATCGCGGTAAACGGCTGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAAcgcgc‑g                                                                                                                                                                                                                                      >  2:344040/1‑251 (MQ=255)    gTGCCTGACCTACGCCGTAGTCACCCAGCACTTGTCTGACCACGCCCCCCGCACCAATCCCCAGCAGAATGATCCCCGCCGGTTTCAGCGCGTGACCGCAAATCCCCACCACTTTGGCTTTTGGCATGCCCGGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGGCCGATAAACTCAACCCATTCGTAAGCAGTTGATCCTTCCGGCGCAATACGCGC‑GGCa                                                                                                                                                                                                                                   <  2:257513/251‑1 (MQ=255)    gTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGCACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGTTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGAAGTTGATCCTTCCGGCGCAAAACGCGA‑GGc                                                                                                                                                                                                                                    >  2:367620/1‑250 (MQ=255)           aCCTACGCCAGAGTCAACCAGCCCCTGTTTGAACACGCCGCCCGCACCAATCCCCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCATAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGttt                                                                                                                                                                                                                            <  1:292931/251‑1 (MQ=255)               gcGCCAGAGCCAACCAGCACGTGTTTGAACACCGCGCCCGCACCAATCGCCAGCAGAAGGATCCCCGCCGGGTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCAAGCGCTGACGCAGGGCCAGTCCGTAAAGCGCCACCAGACAAGCAAACATAATCGCTGTAAACGTATGACCGATAAGCTCAAACCAATCGTAAGCAGGTGAGCCTTCCGTCGCAAAACGCGCTGGCA‑TGGTTTTCa                                                                                                                                                                                                                         <  2:239205/249‑1 (MQ=255)                       gTCAACCAGCACCGGTTTGAACACGCCGCCCGCACCAATCACCAGCAGACTGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGTATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTacca                                                                                                                                                                                                                <  1:355204/251‑1 (MQ=255)                               gCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTaccagcaccagc                                                                                                                                                                                                        >  1:390491/1‑251 (MQ=255)                                cACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCg                                                                                                                                                                                                       >  2:80323/1‑251 (MQ=255)                                 accggttggAACACGCCGCCCGAACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCTCTACCAGCACCAGCgg                                                                                                                                                                                                      <  1:210859/243‑1 (MQ=255)                                          aacccgccgcCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTAGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGAt                                                                                                                                                                                             <  1:91538/247‑1 (MQ=255)                                          aaCACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGAt                                                                                                                                                                                             >  1:587269/1‑251 (MQ=255)                                             ccgccgcCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAg                                                                                                                                                                                          <  1:161610/250‑1 (MQ=255)                                                 cgcccgcACCAATCCCCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCTCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGAc                                                                                                                                                                                      <  1:26056/251‑1 (MQ=255)                                                 cgcccgcACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAACCCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCCATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGAc                                                                                                                                                                                      >  2:496275/1‑251 (MQ=255)                                                           aaTCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATc                                                                                                                                                                            >  1:45258/1‑251 (MQ=255)                                                              cACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCg                                                                                                                                                                          <  1:367390/250‑1 (MQ=255)                                                              cACCAGCAGAATGATCCCCGACGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCg                                                                                                                                                                          >  1:148024/1‑250 (MQ=255)                                                               cccAGCAAAATGCACCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGaa                                                                                                                                                                        <  2:148024/250‑1 (MQ=255)                                                                ccAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGaa                                                                                                                                                                        >  1:456495/1‑250 (MQ=255)                                                                   gcagAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATAGCCACCAGACAAGCAACCAGAATCGCGGAAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCCGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGaa                                                                                                                                                                        >  1:58344/1‑247 (MQ=255)                                                                      aaaTGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGc                                                                                                                                                                 <  2:145292/250‑1 (MQ=255)                                                                           aTCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGCATCCGAAAGATg                                                                                                                                                                   >  2:149900/1‑244 (MQ=255)                                                                           aTCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATg                                                                                                                                                                   <  1:149900/244‑1 (MQ=255)                                                                               ccgccgGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCAC‑GGCAATGGTTTTCAGCCCTACCAGCACCCGCGGCAGCAGGATCAGCGACAGGCTGAATACGGAAGATGGCATTTTGCCt                                                                                                                                                        >  1:313909/1‑251 (MQ=255)                                                                                cgccgGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCtt                                                                                                                                                       >  1:479550/1‑251 (MQ=255)                                                                                          agagcgTGACCGCAAATCTACATCACGTTGTCCTGTGGCATGCCCTGACGCAATGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAATGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTGTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGAta                                                                                                                                             <  2:214558/248‑2 (MQ=255)                                                                                                 gACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGTGGTTc                                                                                                                                       <  2:111266/250‑1 (MQ=255)                                                                                                 gACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTc                                                                                                                                       <  1:542925/250‑1 (MQ=255)                                                                                                  aCCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCATCCAGAATCGCGGTAAACGGATGACCGCTAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCCTTTTGCCTTCGCCGAGATGCGGTTCGc                                                                                                                                     >  2:462705/1‑251 (MQ=255)                                                                                                    cGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTg                                                                                                                                   <  1:261312/251‑1 (MQ=255)                                                                                                      cAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTACATCGCCACCAGTCAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGAt                                                                                                                                                                    >  2:201710/1‑216 (MQ=255)                                                                                                      cAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGAt                                                                                                                                                                    <  1:201710/216‑1 (MQ=255)                                                                                                         aTCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGAGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtc                                                                                                                              >  2:569243/1‑251 (MQ=255)                                                                                                              cATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCTATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGGCGTCAg                                                                                                                         <  2:475055/251‑1 (MQ=255)                                                                                                                tCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAgg                                                                                                                        >  2:525256/1‑250 (MQ=255)                                                                                                                   cTTTGTCTTTTGGCATGCCCTGAAGCATTGCCAGACCGTACATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATAAGCGCCAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGCTGCGGTTCGCTGATGTCGTCAGGAat                                                                                                                     >  2:497196/1‑250 (MQ=255)                                                                                                                      tGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCCGGATCAGCGACAGGCTGACTCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGc                                                                                                                 >  1:235794/1‑251 (MQ=255)                                                                                                                       gTCTGTTGGCATGTCCTGACGCATTGCCAGACCGGAAATCTGCGCCAGGCAAGCAACCAGAATCGCGGTAAAGGGATGACGGAGAATCGCAAACCATTCGTAAGCATTTGATCGTTCCGGCGCAAAACCCGC‑GGCAATGGGTTGCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCGGAATCCGAAAGATCGCATGTTTCCTACGCCGAGATGCGGTTCTATGTTGTCGTCAAGAAGATGCa                                                                                                                <  2:241136/251‑1 (MQ=255)                                                                                                                             tGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCa                                                                                                           <  1:389689/250‑1 (MQ=255)                                                                                                                              ggCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCaa                                                                                                          <  2:456495/250‑1 (MQ=255)                                                                                                                               gCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACg                                                                                                        <  1:525256/251‑1 (MQ=255)                                                                                                                                   gCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAAc                                                                                                    >  1:477544/1‑251 (MQ=255)                                                                                                                                    cccTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCTTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACg                                                                                                   <  1:284447/251‑1 (MQ=255)                                                                                                                                    cccTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACACAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGGCAGGAATATGCAGCTCAACGTAACg                                                                                                   <  2:466624/251‑1 (MQ=255)                                                                                                                                     ccTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACgg                                                                                                  >  2:136468/1‑251 (MQ=255)                                                                                                                                     ccTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACgg                                                                                                  <  1:422135/251‑1 (MQ=255)                                                                                                                                          cGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCtgat                                                                                             <  1:569243/251‑1 (MQ=255)                                                                                                                                                  cAGACCGGAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTGCCGGCGCAAAACGCGC‑GGCAATGGTTTGCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCGCAGCGTAACGGCTGATGAAGttcc                                                                                     <  2:107458/251‑3 (MQ=255)                                                                                                                                                   aGACCGTAAATCGCCACCAGACAAGCAACCAGAAGCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTAcc                                                                                    <  1:59472/251‑1 (MQ=255)                                                                                                                                                          aaaTCGCCACCAGACAAGCAACCAGAATCTCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGc                                                                             <  2:447135/251‑1 (MQ=255)                                                                                                                                                               gCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGccc                                                                        >  2:47339/1‑251 (MQ=255)                                                                                                                                                                 caccaGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGACGAATTTACCCCACAGCGGCCCgg                                                                      <  2:363663/251‑1 (MQ=255)                                                                                                                                                                    caGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCACTGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAGGTTACCCCACAGCGGCCCGGCa                                                                    >  1:239795/1‑250 (MQ=255)                                                                                                                                                                     aggcaagaaacaagacttgcgctAAACGGGTAACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGAGA‑GGCAATGGTTTTCAGCCCTACCAGCACCACCGGCAGCAGGATCAGCGAAAGGCTGCATCAGACAGATGGCCTTTTGCCTTCGCCGGGCTGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTAAAAGTACCGGCTGATGAAGTTCACCCACAAAGGCCCGGCaat                                                                  >  2:47215/23‑251 (MQ=255)                                                                                                                                                                      gACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCaat                                                                  >  1:417271/1‑250 (MQ=255)                                                                                                                                                                               ccAGAATCGCGGTAACCGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTccc                                                        >  1:160226/1‑251 (MQ=255)                                                                                                                                                                                     tCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAAtt                                                  >  2:238882/1‑251 (MQ=255)                                                                                                                                                                                             aaCGGAAGACCGATAAACTCAAACCATGCGGAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCGGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGGTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGTCAATAATCATTCCCGGAATTGCCGcaca                                          <  2:307849/251‑1 (MQ=255)                                                                                                                                                                                                  aTGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGc                                     <  2:421406/251‑1 (MQ=255)                                                                                                                                                                                                   tGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACCCAGGcc                                    >  1:316601/1‑251 (MQ=255)                                                                                                                                                                                                    gACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCCCCAGCTGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGTCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCa                                   <  2:479550/251‑1 (MQ=255)                                                                                                                                                                                                    gACCGATAAACTCAAACCATTCGTAAGCAGTGGATCCGTCCGGCGCAAAACGCGC‑GGCAATGGTTGTCAGCCCTCCCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTGCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAAGCATTACCGGAATTGCCGCACACAGGCCa                                   <  2:496977/251‑1 (MQ=255)                                                                                                                                                                                                       cGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATc                                <  2:193021/251‑1 (MQ=255)                                                                                                                                                                                                            aaCTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGa                            >  1:404039/1‑250 (MQ=255)                                                                                                                                                                                                                cAAACCAGTCTTAAACAGTTTATCCTTCCGGCGCAAAACGCGC‑GGCCATGGTATTCATCCCTACCATCTCCAGCGGCAGCTGTATCAGCGACAGGCTGAATCCGAAAGAGGGCTGTTTGCCTTCGCCGCGATGCGGTTCGCTGACGTCGTCAAGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCTCAGCGGCCCCGCAATAATCATTCCCTGAATTGCCGCCAACAGGCCAATCAGGatcag                        <  2:312211/250‑2 (MQ=255)                                                                                                                                                                                                                       ttCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACCCAGGCCAATCAGGATCATCCAGCCa                 >  2:137409/1‑250 (MQ=255)                                                                                                                                                                                                                       ttCGGAAGCACTTGAGCCTTCCGACGCAAAACTCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGCTGCGGTGCTCTGATGTCGTGAGGAATATGCAGCTCATCGTAACGGCTGATGAAGTTACCCCACCGCGGCCCTGCAATAATCATTCCCGGGATTGCCGCCCCCAGACCAATCAGGCTCATCCAGCCac                >  2:211268/1‑250 (MQ=255)                                                                                                                                                                                                                          gTAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCACTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCaa                >  1:352040/1‑248 (MQ=255)                                                                                                                                                                                                                           tAAGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTc            >  2:183557/1‑251 (MQ=255)                                                                                                                                                                                                                            aaGCAGTTGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCg           <  1:490233/251‑1 (MQ=255)                                                                                                                                                                                                                            aaGCAGTTGATCCTTACGGCGCAAACCGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGAAAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGCATATGCAGATCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCCGGATCCTCCAGCCAAAGTCg           >  2:95161/1‑251 (MQ=255)                                                                                                                                                                                                                                aGTTGATCCTGCCGGCGCAAAACGCGC‑GGCAATTGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCAt       <  1:109308/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttGATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGACAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCACTAATCATTCCCGGAATTGCCCCCCACAGGCCAATCAGGAGCACCCAACCAAAGACGGCATTc     >  2:426348/1‑251 (MQ=255)                                                                                                                                                                                                                                    gATCCTTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGCTGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCACTAATCATTCCCGGAATTGACGCCCACACGCCCATCAGGATCATCCACCCAAAGACGGCAATCac   >  2:321630/1‑250 (MQ=255)                                                                                                                                                                                                                                     aTCCGTCCGGCGCAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATc  <  1:344040/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 CCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCACAAAACGCGC‑GGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATC  >  NC_000913/3576074‑3576552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑