Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,558,291	C→T	G8G (GGC→GGT)	rtcR →	DNA‑binding transcriptional activator RtcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,558,291	0	C	T	100.0%	163.2 / NA	47	G8G (GGC→GGT)	rtcR	DNA‑binding transcriptional activator RtcR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/16);  total (31/16)

GGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAG  >  NC_000913/3558046‑3558526                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              gggCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACAGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGt                                                                                                                                                                                                                                          >  2:531120/1‑251 (MQ=255)     aTTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATTCGTAAAACAGTGGCTTTTGGTTTTGTCGGt                                                                                                                                                                                                                                      <  1:112219/251‑1 (MQ=255)          cAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCg                                                                                                                                                                                                                                  <  1:130724/250‑1 (MQ=255)                 cAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGAt                                                                                                                                                                                                                          >  2:519732/1‑251 (MQ=255)                              ttcATTTTGTTTTTCTGTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAGAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTTTCGGTACCGTACTGGATTATGCCGGG‑cgcg                                                                                                                                                                                                            >  2:338660/1‑251 (MQ=255)                                            ctctTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTAAGCGCTgg                                                                                                                                                                                              >  2:529104/1‑251 (MQ=255)                                              ctTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTg                                                                                                                                                                                               <  1:247219/248‑1 (MQ=255)                                               ttttCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTc                                                                                                                                                                                            >  1:411907/1‑250 (MQ=255)                                                      tgttTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATggg                                                                                                                                                                                    >  2:186550/1‑250 (MQ=255)                                                          tGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTcc                                                                                                                                                                                >  2:450322/1‑251 (MQ=255)                                                           gatGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTcc                                                                                                                                                                                <  1:105981/248‑1 (MQ=255)                                                            cTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACAGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCg                                                                                                                                                                               >  2:133627/1‑250 (MQ=255)                                                                                               tAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATCTCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGACAATTTACAGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCggg                                                                                                                                                                                                                 >  2:62955/1‑182 (MQ=255)                                                                                               tAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCggg                                                                                                                                                                                                                 <  1:62955/182‑1 (MQ=255)                                                                                                aaaTAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatcg                                                                                                                                         >  1:414859/1‑251 (MQ=255)                                                                                                      gcagcTTAATTTTTTAATTCATTGTTTTAAAAACGATTATATCTTTACGACCGTAACCGTAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTTTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCCGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTAGGTCATCGATCGAc                                                                                                                                   >  2:580588/1‑251 (MQ=255)                                                                                                             aaTTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGCGATTTCTCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAACTGGCGTCCGTAC‑CTCTGTTTATGCCCGCAAGAATCGTTGGTCATCGATCGACTGGAAtt                                                                                                                            >  2:197822/1‑251 (MQ=255)                                                                                                                             gTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgc                                                                                                            >  1:523644/1‑251 (MQ=255)                                                                                                                               tttAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGCCTGGAATTGTTGCACGACGCCcgctc                                                                                                          >  2:445461/1‑251 (MQ=255)                                                                                                                                   aaaaaGTTTATCTCTTTACGTCCGTAACCGGTGATTTCCCGCAAAGCCAATTTACCGATAATGCAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAACCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGAGCTGGTCAAAATGGCGTCCG‑CCACTCTGTTTATGCCAGCAAGAATCGTTGGTCCTCGCTCGACTGGCATTGTTGCACGCCGCCcgctcgcgc                                                                                                      >  2:88535/1‑251 (MQ=255)                                                                                                                                           tataTCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTAtt                                                                                              >  1:510907/1‑251 (MQ=255)                                                                                                                                           tataTCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTAt                                                                                               <  2:450110/250‑1 (MQ=255)                                                                                                                                            atatCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTAttt                                                                                             <  2:169039/251‑1 (MQ=255)                                                                                                                                                cTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTACAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCTGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGaa                                                                                          >  1:105031/1‑250 (MQ=255)                                                                                                                                                   tACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGc                                                                                      >  1:411995/1‑251 (MQ=255)                                                                                                                                                      gTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCtt                                                                                    >  1:455460/1‑250 (MQ=255)                                                                                                                                                          gTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAAcg                                                                               <  1:223456/251‑1 (MQ=255)                                                                                                                                                            aaCCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAAcgc                                                                              <  2:164816/250‑1 (MQ=255)                                                                                                                                                                           gCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATTAGGATATCTAAGATGCGTAAAACAGTGGATTTTGGTTTTATCGGTACCGAACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGAGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGGTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGAGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGtgt                                                              >  1:235120/1‑249 (MQ=255)                                                                                                                                                                              aaGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCAGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGc                                                           >  2:416480/1‑251 (MQ=255)                                                                                                                                                                                  cAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGcc                                                          >  1:235312/1‑248 (MQ=255)                                                                                                                                                                                   aaTTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGa                                                       >  1:502040/1‑250 (MQ=255)                                                                                                                                                                                    aTTTACCGATAATGACATATCGTCTTTTATAAGGATATCTAAGATGCGCAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGAGGCAGTCAGCGCTGGTCAAAATGACGTCCG‑ACACCCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTACACGCGCTATCGCCAGCGTTTCGCCAGaa                                                      >  2:499632/1‑250 (MQ=255)                                                                                                                                                                                           gATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAgt                                              <  2:502040/251‑1 (MQ=255)                                                                                                                                                                                            aTAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGATTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGTCAGCGTTTAGCCAGAAACAGAAggg                                             >  1:135825/1‑249 (MQ=255)                                                                                                                                                                                              aaTGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCGTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAgtgg                                            <  2:488529/250‑1 (MQ=255)                                                                                                                                                                                               aTGAAATACCGTCTTTTATAAGGATTTTTAAAGTGCGTAAACCGTTGGTTTTTGGTTTTTCCGGT‑CCGTTCGTGAATAAGCCGGGTCGCG‑CAGTCAGCGCGGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGa                                         <  2:318985/251‑1 (MQ=255)                                                                                                                                                                                                   aaTATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCg                                      >  2:449871/1‑251 (MQ=255)                                                                                                                                                                                                   aaTATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCg                                      >  1:475718/1‑251 (MQ=255)                                                                                                                                                                                                         gTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCAGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTg                                   >  2:486032/1‑248 (MQ=255)                                                                                                                                                                                                               tataAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCATTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGaa                           <  2:114045/250‑1 (MQ=255)                                                                                                                                                                                                                   aGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAACCAGAAGGGGTGAGCGTTGAGATTGAACTGc                       >  2:130124/1‑250 (MQ=255)                                                                                                                                                                                                                            aaGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACTCTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCg               <  2:411907/249‑1 (MQ=255)                                                                                                                                                                                                                                 gCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGATGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGAt         <  1:450322/250‑1 (MQ=255)                                                                                                                                                                                                                                  cGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGAttt       <  1:338660/251‑1 (MQ=255)                                                                                                                                                                                                                                        aCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGa    >  1:390977/1‑248 (MQ=255)                                                                                                                                                                                                                                         cAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAag  >  1:341070/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              GGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGG‑CGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCG‑ACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAG  >  NC_000913/3558046‑3558526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑