Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	209.9 / NA	63	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/31);  total (32/31)

GCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGC  >  NC_000913/3329503‑3329985                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   gCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTaa                                                                                                                                                                                                                                           <  1:136293/250‑1 (MQ=255)  ccccAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTaaa                                                                                                                                                                                                                                          >  2:130567/1‑250 (MQ=255)   cccAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGtt                                                                                                                                                                                                                                             <  2:419440/246‑1 (MQ=255)   cccAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGtt                                                                                                                                                                                                                                             >  1:419440/1‑246 (MQ=255)       aaGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGc                                                                                                                                                                                                                                    <  1:430113/251‑1 (MQ=255)                         tttATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCgg                                                                                                                                                                                                                  >  1:454887/1‑251 (MQ=255)                         tttATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCg                                                                                                                                                                                                                   <  1:214073/250‑1 (MQ=255)                            ataCGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGGGCGGAac                                                                                                                                                                                                               >  1:572183/1‑251 (MQ=255)                               cgcgTGGAATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGCGTTAAAACAGGCGGGTAACACCTGGAGCGGAACAct                                                                                                                                                                                                            >  1:224646/1‑251 (MQ=255)                                   gggCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACActgctg                                                                                                                                                                                                        <  1:484755/250‑1 (MQ=255)                                   gggCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACActgctg                                                                                                                                                                                                        <  1:436192/250‑1 (MQ=255)                                                ccccGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAgg                                                                                                                                                                                           <  2:451621/251‑1 (MQ=255)                                                        cGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATTCCCTGGGGCGGGACAATGCTGCGCCAGAcaccgggtaacgc                                                                                                                                                                                    >  1:529659/1‑238 (MQ=255)                                                           tGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACCCAGGTTAACGAACCt                                                                                                                                                                                >  1:435297/1‑251 (MQ=255)                                                                 gCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTCCGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCCCCTGGAGCGGGAAACTGCTGCGCCCGACACCGGTTAACCAACCTGGGACg                                                                                                                                                                          >  2:74589/1‑251 (MQ=255)                                                                         cGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCCCCTGGAGCGGAACAATGCCTCGCCAGACTCAGGTTTAAGAACCTGGAAAGGGAGtttc                                                                                                                                                                  >  2:398719/1‑249 (MQ=255)                                                                              ccTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTa                                                                                                                                                             <  2:1278/251‑1 (MQ=255)                                                                                ccccccGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTaaa                                                                                                                                                           <  1:285383/250‑1 (MQ=255)                                                                                                       cAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGTGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTATTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGTTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCt                                                                                                                                    <  2:393329/251‑1 (MQ=255)                                                                                                        aaTACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCCGCACGATCTg                                                                                                                                   >  1:514901/1‑251 (MQ=255)                                                                                                            cTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAAGCGGGTATCACCTGGAGAGGACCACTGCTGCGCCCGACTCAGGTTAACGAACCTGGAACGGGAATTGGCAGAAAACACTACGACCCGCTGCCCGATCTGCtt                                                                                                                                >  1:509026/1‑250 (MQ=255)                                                                                                               cGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTGGAGCGGGACCAAGCTGCGCCAAACTCCGGTTAACGAACCTGGAACCGAAGGTGCCAGTAAACAGTCCGCCCCGCTGCACGATCTGATTaaa                                                                                                                             >  2:402650/1‑249 (MQ=255)                                                                                                                 aaCTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTAACAACTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTTCCAGTAAACAGTCGGCCCCGCTGCACGAGCTGCTTAAGAtt                                                                                                                          >  2:221429/1‑251 (MQ=255)                                                                                                                  aCTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCGCCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTa                                                                                                                         <  1:579635/251‑1 (MQ=255)                                                                                                                   cTGGATGAGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTAt                                                                                                                        >  1:156098/1‑251 (MQ=255)                                                                                                                      gATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAATGCTGCGCCCGACTCAGGTTAACCAACCTTGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCt                                                                                                                     >  1:220350/1‑251 (MQ=255)                                                                                                                           ggagCCAGGAGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAAAGTTCTGAGCCGATCCCGTTGGCTTTTGCCGTGCCGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAACTGGAGCGGGACACTGCTGAGCAAGACTCAGAGTAAAGGACCTGGAACGGTAGTTGCCAGTAACCAGTCGGCGCCGCAGCCCGCACTGCTTAAGATTTTGCTGaga                                                                                                                 >  2:540899/4‑248 (MQ=255)                                                                                                                                 aGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTGTCACCCGGAGCTGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGTAACGGTCGGTGCCAGTAAACAGGCGGACCCGCCGCACGATCTGCTTTAGATTATGCTGAAAAAGTCgg                                                                                                          >  2:170267/1‑251 (MQ=255)                                                                                                                                      cccTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGacaac                                                                                                     <  1:36885/250‑1 (MQ=255)                                                                                                                                       ccTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGacaaca                                                                                                    <  1:308765/251‑1 (MQ=255)                                                                                                                                         tGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGCGCCAGCTAAGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTCGAGCGGAACACTGCTGCGCCAGACCCCGGGTAACGAAACTGGCACGGGAGTTGCCAGTAAACAGCCGGCCCCGCCGCACGATCCGCTTAAGGTTATGCTGAAAAAGTCTGACAACATg                                                                                                  >  1:579647/1‑251 (MQ=255)                                                                                                                                                    aCGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGCTAc                                                                                       >  1:200573/1‑251 (MQ=255)                                                                                                                                                      gCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACgg                                                                                     <  2:7012/251‑1 (MQ=255)                                                                                                                                                          aCGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGgttt                                                                                 >  1:320222/1‑251 (MQ=255)                                                                                                                                                             ggATGACTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATCTGAGTTAAAACAGGCGGGTATCACCTGGAGCGGGACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGCGTTGCCAGTAAACAGTCGGCCCCGCCTCACGATCTGCTTAAGCTTTTGCTGAAACAGTCGGACAACATGATAGCCGCTACGGTTTTCCg                                                                              >  2:394707/1‑251 (MQ=255)                                                                                                                                                                 gcctgccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCAt                                                                           <  2:450901/250‑1 (MQ=255)                                                                                                                                                                 gcctgccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATg                                                                          <  1:508641/251‑1 (MQ=255)                                                                                                                                                                  cctgccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGAGGAACACTGCTGCGCCAGACTCAGGGTAACGAACCTGGGACGGGAGGTGCCAGTGAACAGTCGGCCCCCCTGCCCGATCCTCTTAAGATTATGCTGAAAAAGTCGGCAAAAATGGTCGCCGATAAGGGTTTCCGcc                                                                            >  2:318913/1‑247 (MQ=255)                                                                                                                                                                     gtcacaACGTTCTGAGCCGATCCCGTTGTCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGTGTTAAAACAGGCTGGTACCACATGGAGCGGAACACTGCTGCGCCAGTCTCAGGTTACCTAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCGCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGGCAACATGATCGGCCATACGGGTTTCCGCATGATa                                                                       <  2:109276/248‑1 (MQ=255)                                                                                                                                                                         caACGTTCTGAGCCGCTCCCGTTGACTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCCGAACACTGCTGCGCCAAACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAAAGTCGGCCCCGCTGCACCATCTGCTTAAAATTCTGCTGAAACAGACGGACAACATGAACGCCGATACGATTTTCCGCATGATAGGcc                                                                   >  1:9305/1‑250 (MQ=255)                                                                                                                                                                            cGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCCGACTCAGGTTAACGAACCTGGGACGGTAGTTGCCAGCAAACAGTCGGCCCCGCTGCCCGATCTGCTTTAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGAAACGGTTTTCCGCATGATAGGCCCTgc                                                               >  2:485652/1‑251 (MQ=255)                                                                                                                                                                               tCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGGTACGGTTTTCCGCATGATAGGCCATgcgcg                                                            >  1:570909/1‑251 (MQ=255)                                                                                                                                                                                 tGAGCAGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTAACACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGGAAACAGTCGGCCCCGCTGCACGCACTGCTTCAGATTTTGCTGAAAAAGTCGGACCACATGATCGCCGATACGGGTTTCCGCCTGTTAGGCCATGCGCGCt                                                          >  2:401186/1‑251 (MQ=255)                                                                                                                                                                                  gAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGTCATCATGATCGCCGATACGGTTGTCCGCATGATAGGCCGTgcgcg                                                            <  2:274107/248‑1 (MQ=255)                                                                                                                                                                                  gAGCCGCTCCCGGTTGCGTTTGCCGTGCAGGATGGAGCCATCTATGCCGGTGCAATTCTGAAATAGGAGTTAAAACAGGCGGTTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTATCGATCCTGGAACGGTAGTTGCCAGTAAACAGTAGGCTCCGCTGCACGATCTGTTTAAGATTATTCTGATAAAGTCGGACAACATGATCGCCGATAGGGTGTTCCTCATGATAGGCTATGTGCGCt                                                          <  2:586914/250‑1 (MQ=255)                                                                                                                                                                                         tCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAAACCTGCTGCGCCAGACTCAGGGTAACGAACCTGGAACGGTAGTTACCAGCAAACAGTCGGCCCCCCTGCACGCTCTGCTTAAGATTATTCTGAAAAAGACGGGCAACATGATCTCCGATTCGGTATTTCGCATGAAAGGCCCAGCGCGCTTccctgtg                                                  >  2:23431/1‑245 (MQ=255)                                                                                                                                                                                          cccGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAAtgt                                                   <  2:220350/249‑1 (MQ=255)                                                                                                                                                                                          cccGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAAtgt                                                   <  1:446258/249‑1 (MQ=255)                                                                                                                                                                                                 gCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAAc                                          <  2:570909/251‑1 (MQ=255)                                                                                                                                                                                                    tttGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACAt                                        <  1:328109/250‑1 (MQ=255)                                                                                                                                                                                                      tGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCCGGAGCGGAACACTGCTGCGCCAGACTCAGGGTAACGCACCTGGGACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTTCACGATCTGCTTAAGATTTTGCTGAAAAAGTCGGACAACATGATCGCCGATACGGGTTTCCGCATGCTAAGCCATGCGCGCTTTAATGTGCCTGGAACATGGc                                     >  2:243194/1‑251 (MQ=255)                                                                                                                                                                                                        ccgtggaggcaggAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGCATCACCTGGGGAGGAACACTGCTGCGCCAGACAACGGGTACCGAAACTGGAACCGGAGTTTCCAGTAAACAGTCGGCCCCGGTGCACGATCTGCTTAAGATTATAATGACAAAGGCGGACAACATGATCGGCGATGAGGTATTCCGCCTGATAGGCCATGCGCGCTTCAATGTGACTGGAAAATGGCgg                                   >  2:244229/12‑251 (MQ=255)                                                                                                                                                                                                         cGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTTTCACCTGGCGCGGGACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCCGGAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACCTGATCGCCGATACGGTTTTCCGCATGTTAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCggg                                  >  2:483487/1‑251 (MQ=255)                                                                                                                                                                                                              aGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATTGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCggg                             <  1:265216/251‑1 (MQ=255)                                                                                                                                                                                                                   ggAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGa                        <  2:435297/251‑1 (MQ=255)                                                                                                                                                                                                                       ccAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGcc                    <  2:176193/251‑1 (MQ=255)                                                                                                                                                                                                                               gCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTc             <  2:103784/250‑1 (MQ=255)                                                                                                                                                                                                                                  ggTGCAATTCTGAAATATGAGTAAAAACAGGCGGGTATCACCCGGATCGGAACACTGCTGCGCCAGACTCGGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCGGCACGACCTGCTTAAGTGTATGCTGAAAAAGTCGCACAACATGATCGCCTATACGCTTTTCCGCATGATATGCCATTCGCGCTTCAATGTGCCGTGAATATGTGGGGCCGTTTCGGACGGCGTGCGTCAGAt         <  2:554978/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCAATTCTGAAATATGAGATAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCt      <  2:15585/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaTTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCGGCGCCAGACTCAGGTTAACGAACCGTGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCGTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTCCGTCAGATCCTgc    <  2:49984/251‑1 (MQ=255)                                                                                                                                                                                                                                        aTTCTGAAATATGAGTTAAAACCGGCGGGTATCACCTGGAGCGGCACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGAAAACAGTCGGGCCCGGTGCACGATCTGCATAAGAATCAGCTGACAACGGCGGACAAAATGATCGCCGAAAAGGTTTTCCGCATGCAAGGCCATGCGCGCCTCAATGTGCCTGGAACATGGAGGGGCGGGACGGACCCCGTGCGTCaaaacctacc   >  1:503653/1‑242 (MQ=255)                                                                                                                                                                                                                                        aTTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTTCCAGTAAACAGTCGGCCCCGCCGCACGGTCTGCTTAAGATTATGCTGAAAAAGTAGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCAAGCGCGCTTCAATGTGCCTGGAACATGGCGGGGCGGGTCggcccgccggcgttcgatcctgcg   >  1:115873/1‑229 (MQ=255)                                                                                                                                                                                                                                          tCTGAAATATGAGTTAAAACAGGCGGTTATCACCTGGAGCGGAACACTGCTGCGCCAGACGCATGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATTCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTgcgc  <  1:540899/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   GCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGC  >  NC_000913/3329503‑3329985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑