Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,270,497	C→T	noncoding (96/377 nt)	rnpB ←	RNase P catalytic RNA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,497	0	C	T	95.1%	121.0 / ‑6.1	41	noncoding (96/377 nt)	rnpB	RNase P catalytic RNA component
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (11/28);  total (12/29)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑CCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACG  >  NC_000913/3270278‑3270742                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          gCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGACCCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTGCCCCCCCAGGCGTTA‑TCTGGCTCCCTGCCCTATGG‑AGCCCGGACttt                                                                                                                                                                                                                           <  2:147637/251‑1 (MQ=255)        ggCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTcc‑tcccc                                                                                                                                                                                                                   <  1:61978/251‑1 (MQ=255)                    cTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTTTCTGTT‑TCACT‑TGTGGGGGG‑GGTCCCCCCCCCGCGTTA‑TCTTGCGCCCCGCCCCATGGGAGCC‑GGCCTTTcc‑tccccccccccccgccc                                                                                                                                                                                                       >  1:559342/1‑239 (MQ=255)                                aGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGCGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTCTCTCTT‑GTCCC‑GGTCGTTGG‑GTTCCCCCCCCGGCGGTC‑TCTGGCCCCCCGCCCCATGG‑GGCCCCGGCTTTCC‑TCCCCCCCCCCCCCC‑Tccccccaacagg                                                                                                                                                                                           >  2:575500/1‑243 (MQ=255)                                       gCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTTTT‑GCCCT‑GGTCGTGGGGTTTCCCCCCC‑GGCGTTT‑TCTGGCACCCTCCCCCTTTG‑AGCCCGGACTTTCCCTCCCC‑CCCCCCGTC‑TCCCCCCCAGAGgacggcgac                                                                                                                                                                                   >  1:108553/1‑251 (MQ=255)                                                tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAgcggc                                                                                                                                                                           <  1:281937/250‑1 (MQ=255)                                                 aTGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAgcggcg                                                                                                                                                                          <  2:126805/250‑1 (MQ=255)                                                 aTGAACCCCTATTTGGCCGTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAgcggcg                                                                                                                                                                          <  2:429035/250‑1 (MQ=255)                                                   gAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTTCCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACt                                                                                                                                                                       <  2:571688/251‑1 (MQ=255)                                                          tttttGGC‑TTGCCCCCGGTGGAGTTTTACGTGCCACGGACTGTTTCCAGCCCCGCGGGGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTgg                                                                                                                                                                 <  2:206434/247‑1 (MQ=255)                                                                   ttGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTT‑TC‑CTCGGGCGTGGG‑TTTCCCCCCCCGGGGGTATCTTGGC‑CCCCGCCCCATGG‑GGGCCGGGCCTTCC‑TTCCCCCCCCCCGCC‑CCCCCCCCAGAAGGCGACGACGAAACGGCCACCGTCTGGTCAGCTTccg                                                                                                                                                       >  1:550106/1‑249 (MQ=255)                                                                    tGCTCCGGGTGGATTTTACCGGGCCACGGACTGTTACCAGCCGCGCGGGGCGCTCTTACCGCCCTCTTTCCCCCTTACCTGATCCCGCTTGCGCGGGCCAGCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCGATGG‑AGCCCGGACTGTCC‑GCCCCTCCGCCCGTC‑TCCCCCGAAGATGACGACGACGAAGCGTCGACTGTCTGGTCAGCTTCgg                                                                                                                                                       <  2:350196/250‑1 (MQ=255)                                                                          gggTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGATTGTTCTCTGTT‑TACCT‑GGTCGTGGGGTTTCCCCCCC‑GGGGTCA‑TCTGGCACCCCGACCACTGG‑AGCCCGGACTTTCT‑TCCCCCCCCCCCGCC‑TCCCCCGAAGAGGGAGACGACATAGCGGGGACCGCCTGGTCAACTTCggggggga                                                                                                                                                 >  1:542923/1‑244 (MQ=255)                                                                                          gCCACTGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCTCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTgcg                                                                                                                                <  2:24749/250‑1 (MQ=255)                                                                                                         cAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTTTCTGTTTGCCCT‑G‑TCGTGGGGTTTCCCCCCC‑GGCGTTC‑TCTTGCCACCTGCCCCCTTG‑AGCCCGGGATTTCC‑TCCCCCCCCCCCGCCCTCCCCCGAA‑AGGGCGACGCCGAAAAGGGGCCCGTCTGGTCAACTTCGGCGGGCCGGATATGAGGGtttcccgccccttttcccccc                                                                                                                 >  1:244544/1‑233 (MQ=255)                                                                                                            ccGCGCGGTGCGCGCGTACCGCACCCTTTCACCCTTACCTGATCCCGCTGGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTg                                                                                                                                  <  1:499094/230‑1 (MQ=255)                                                                                                                  ggttcgctcctcccgctcacTTTCCCCCTTTCCTGTTCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGTCTTTCC‑CCCCCTCCGCCCGTC‑TCCCCCGAAGAGGAGGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCTCTTGGCACCGCGCGTGGCGCa                                                                                                       <  2:287593/232‑1 (MQ=255)                                                                                                                  ggTGCGCTCTTTCCGGACCTTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCGCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTTGCACCCTTCCCTATGT‑AGCCAGGACTTTCC‑GCCCTTCCGCCCGTC‑TCCCCCGAAGAGTATGACGACGAAGCGGCTACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCa                                                                                                       <  1:528820/251‑1 (MQ=255)                                                                                                                     gcgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTc                                                                                                    <  2:107152/251‑1 (MQ=255)                                                                                                                                  ccccTTTCACCCTTTCCTGATCCCGCTGGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCTCGCCCTTGGCACCCCGCGTTGCGCATTCCAATCGCCAgtgt                                                                                       <  1:193633/250‑1 (MQ=255)                                                                                                                                  aCCCTTTCACCCTTACCTGCTCCCGCTTGCGCGGGCCATCGGCGGTTTGCGTTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGTCCTATGG‑AGCCCGGACTTGCC‑TCCCCTCCGCCCGGC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCTGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAAGCGCCAgtgt                                                                                       <  2:559342/251‑1 (MQ=255)                                                                                                                                       ttCACCCTTTCCCGATCCCGCTTGCGCCGGCCATCGGCGGTTTGCTCGCTGTT‑GCACT‑GGGCGTGGG‑TTTCCCCCCCAGTCGTTA‑TCTGGCTCCCTGCCCGATGG‑AGCCCGGACTTGCC‑CCCCTTCCGCCCGTC‑TGCCCCGAAGAGGACGACGACTAAGCGGCGACTGGCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTGGTCACCCCGCGTTGCGCATGCCAATCGCCAGTGTcgcg                                                                                   <  2:532554/250‑1 (MQ=255)                                                                                                                                       ttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTT‑TCCCT‑GGTCGTGGG‑GTTCCCCCCCCGGCGTTA‑TCTGGCACCCTCCCCTATGG‑GGGCCGGGCTTTCC‑CCCCCCCCCCCCCCC‑CCCCCCCCCGCGGGCGACAACGCAGCGGCGACCGTCCGGGCCCCTTCGGCGCGCCCGATA‑GAGGGGTTGGGCGCCCCTTTCCCACCCCGTTGCGCCTTCCAACCCCCAgtgtaccgg                                                                                  >  2:16134/1‑246 (MQ=255)                                                                                                                                           cccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGat                                                                              <  1:408465/251‑1 (MQ=255)                                                                                                                                               tacttggtCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑GTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑GCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCGTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTGGTCACCCCGCGTTGCGCATTCCAGTCGCCAGTGTCGCGGCGatat                                                                            <  2:391441/242‑1 (MQ=255)                                                                                                                                                          gCTTGCGCGGGCCATCGGCTGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCTCGGCGATATTACGTGAAGCACg                                                               <  1:164607/251‑1 (MQ=255)                                                                                                                                                                         cggcggTTTGCTCTCTGTT‑GCGCT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCGGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑ACCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGTGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGGGTCGCGGCGATATTACTTGAAGCACGGCAGATATTGTCATa                                                <  2:47122/251‑1 (MQ=255)                                                                                                                                                                          ggcggTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGACAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCCATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATa                                                >  1:535/1‑250 (MQ=255)                                                                                                                                                                            cggTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGc                                             <  1:575500/251‑1 (MQ=255)                                                                                                                                                                             ggTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGcc                                            <  1:265622/251‑1 (MQ=255)                                                                                                                                                                                  gCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCcgcg                                       <  1:214878/251‑1 (MQ=255)                                                                                                                                                                                               aCT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTc                           >  2:273931/1‑251 (MQ=255)                                                                                                                                                                                                    gTCGTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAAc                       <  1:515035/251‑1 (MQ=255)                                                                                                                                                                                                       gTGGG‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGACAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGt                     >  2:155488/1‑250 (MQ=255)                                                                                                                                                                                                       gTGGG‑ATTCCCCCCACGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGCCTGTCTGGTCAGCTTCGGCGCGCCGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCCACGt                     >  2:529451/1‑250 (MQ=255)                                                                                                                                                                                                         ggg‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTGTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGGTATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTAc                   <  2:60681/250‑1 (MQ=255)                                                                                                                                                                                                          gg‑TTTCCCCCCCAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCt                 <  1:438794/251‑1 (MQ=255)                                                                                                                                                                                                                  cccccAGGCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTAGA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTgg          <  1:515647/251‑1 (MQ=255)                                                                                                                                                                                                                      cAGGCGTTA‑CCGGGCCCCCTGCCCTAGGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATAGTGTCATAAGCCCCGCGGAAGGCTTCGGCCAACGTGCCTATGCTGGTCaa      <  2:550106/251‑1 (MQ=255)                                                                                                                                                                                                                         gCGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATAc   <  1:383958/251‑1 (MQ=255)                                                                                                                                                                                                                          cGTTA‑TCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCACTACg  >  1:523749/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          GCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTT‑GCACT‑GGTCGTGGG‑TTTCCCCCCCAGGCGTTA‑CCTGGCACCCTGCCCTATGG‑AGCCCGGACTTTCC‑TCCCCTCCGCCCGTC‑TCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA‑GAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACG  >  NC_000913/3270278‑3270742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑