Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,895	C→T	L755L (CTG→TTG)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,895	0	C	T	100.0%	217.1 / NA	68	L755L (CTG→TTG)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/33);  total (35/33)

AACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTG  >  NC_000913/3224652‑3225140                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       aaCATTACGTTGACCATAGATTCCCGCACCCCACACACCGAAGACGGCCCCCCTAAAGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACACAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACAGTTCGCGGCTACAACTTAGCGATCACCTTCTCAAAAATGAGAGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTcg                                                                                                                                                                                                                                                 >  2:339964/1‑251 (MQ=255)              ccAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATc                                                                                                                                                                                                                                   <  1:268738/251‑1 (MQ=255)                       cccGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGCCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACttcttc                                                                                                                                                                                                                          <  1:464869/251‑1 (MQ=255)                        ccGCACCCGCTACAGCGAAGCCGGAAACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAAAGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACGGTTCGCGGCTACAACTTCGCGATCACCTTCTCACAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACttcttc                                                                                                                                                                                                                          >  1:115751/1‑250 (MQ=255)                             cccGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCAAGTGCCTTTCGCACAAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATAACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATGGCTGTTGAATCGCGAGCCAAAG‑ATCAACTTCTTAAAGCCg                                                                                                                                                                                                                    >  1:463036/1‑251 (MQ=255)                              ccGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCga                                                                                                                                                                                                                   <  1:536226/251‑1 (MQ=255)                                   aCAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATc                                                                                                                                                                                                              <  1:138319/251‑1 (MQ=255)                                    cAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCg                                                                                                                                                                                                             <  1:81494/251‑1 (MQ=255)                                                       aTCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCACATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATTGCTGTTGCCTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGt                                                                                                                                                                                           >  2:107767/1‑250 (MQ=255)                                                       aTCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGt                                                                                                                                                                                           >  2:172815/1‑250 (MQ=255)                                                       aTCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGt                                                                                                                                                                                           >  2:228350/1‑250 (MQ=255)                                                                      ttCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGc                                                                                                                                                                            >  1:163136/1‑250 (MQ=255)                                                                          cGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAAGCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCg                                                                                                                                                                       <  2:399768/251‑1 (MQ=255)                                                                             tgaagcaaaccccagcgcAGCCAGAGCCTTTCGCACCAAATAATGCGCGTCAGCTGACGCTGGAAGACGATCGTTAGAGCTGCCCCGTTCGCGACTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACCTCCTGGCAGGTGAATGGCGACTCGCTGTTGACTAGCGGGCCCAAG‑ATCAACTTCTTCACGCCGATGATAGACAACCAAAAGCAGGAGTACGAAGGGCTGCGGCACCCgcat                                                                                                                                                                    >  2:346526/15‑248 (MQ=255)                                                                                   aaaaCACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTg                                                                                                                                                              <  1:136053/251‑1 (MQ=255)                                                                                    aaaCACCGCGCAGCCAGTGCCTTTCGCACCAAATAATTCGCGTCCGCTGACGTTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGc                                                                                                                                                             <  1:16929/251‑1 (MQ=255)                                                                                       cacCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGc                                                                                                                                                             >  2:465342/1‑248 (MQ=255)                                                                                        cccGCGCAGCCAGTGCCTTTCGCCCCAAATAAGGCGCGTCCGCTGACGCTGGAAGACGATCGTTGGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGAt                                                                                                                                                         <  2:229830/250‑1 (MQ=255)                                                                                           gcgcAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGCGGCAACCGAATCATTTGCAGATCAt                                                                                                                                                      >  2:469951/1‑251 (MQ=255)                                                                                            cgcAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATg                                                                                                                                                     <  1:406653/251‑1 (MQ=255)                                                                                               aGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAg                                                                                                                                                  <  1:460105/251‑1 (MQ=255)                                                                                                 ccAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAgg                                                                                                                                                 >  2:296131/1‑250 (MQ=255)                                                                                                       ccTTTAGCACAAAACAATTCACGTCCGCTGACGCTGGAAGACAATAGTTTGAGCTGCAACGTTCACGGCCACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGCATGGCGAATCGCTGTTGACTCGAGAGCCAAAG‑ACCCACTTCTTCAAGCCGATGCTCGCAAACAACACGCAGGAGTACGCCGGGCTGTGGAAACCGAATCATTTGCAGATCATGCAGGAACATCt                                                                                                                                          >  2:559976/1‑251 (MQ=255)                                                                                                            cGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGcgcg                                                                                                                                     <  1:148615/251‑1 (MQ=255)                                                                                                             gCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGc                                                                                                                                                                                                                      >  2:324754/1‑169 (MQ=255)                                                                                                                 cAAATACTGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAACTGAGTGGCAAACCGACATCCTGGCAGGGGAATGGCGAATCGCTGTTGACTCGCGGGACAAAG‑ATCAACTTCTTCAAGCCGCAGATCGACACCCACAAGCAGGAGTACGCAGGGCTGTGGCCACCGAATCATTTGCAGTTCATGCAGGGACCTCTgcgagaattt                                                                                                                                >  2:503549/1‑244 (MQ=255)                                                                                                                 cAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACttt                                                                                                                                >  1:166602/1‑251 (MQ=255)                                                                                                                  aaaTAATGCGCGGCCGCTGACGCTGGAAGACGATCGTTTGAGCGGCACCGTTCGCGGCTACAACTTCGCGAGCACCTTCTCAAAAATGAGGGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACACGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTg                                                                                                                               <  2:116058/251‑1 (MQ=255)                                                                                                                      aatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGt                                                                                                                           <  1:421322/251‑1 (MQ=255)                                                                                                                      aatGCGCGTCCGCTGAAGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCgc                                                                                                                           >  2:111154/1‑250 (MQ=255)                                                                                                                       atGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGt                                                                                                                           >  1:417728/1‑250 (MQ=255)                                                                                                                       atGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGt                                                                                                                           >  2:16160/1‑250 (MQ=255)                                                                                                                           gcgTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAAc                                                                                                                      >  2:464728/1‑251 (MQ=255)                                                                                                                            cgTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGGTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCAGTTGCAGATCATGCAGGAACATCTTCGCGACTTTGCCGTAGAACa                                                                                                                     <  2:406646/251‑1 (MQ=255)                                                                                                                               ccGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACagag                                                                                                                  <  2:203883/251‑1 (MQ=255)                                                                                                                                           gAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACTTTCTCAAAAAGGAGGGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGAGCATGCAGGAACATCTGCGCGACGTTGCCGTAGAACAGAGCGATGGTGAAGt                                                                                                      <  2:162196/251‑1 (MQ=255)                                                                                                                                           gAAGACGATCGTTTGAGCGGCACCGTTCGCGGGTAAAACTTCGCGATCACCTTCTCAAAAAGGAGTGGCAAACAGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑AACAACTTCTGAAAGCCGATGATCGACAACCACAAGCAGGAGGACGAAGGGCTGTGGCAAACGAATCATTTGCAGATCATGCAGGAACAGCTGCGCGACTTTGCCGGAGAACAGAGCGATGGTGAAGt                                                                                                      <  2:115251/251‑1 (MQ=255)                                                                                                                                                     gTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCTATGATTGAc                                                                                                                                                                                                           <  2:34617/140‑1 (MQ=255)                                                                                                                                                     gTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATTGAc                                                                                                                                                                                                           >  1:34617/1‑140 (MQ=255)                                                                                                                                                        tGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGTTGAAGTGCTGATCATCAg                                                                                          <  1:105842/250‑1 (MQ=255)                                                                                                                                                         gAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGATGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGcc                                                                                        >  1:584755/1‑251 (MQ=255)                                                                                                                                                                   ggtCGCGGCTACAACTTCGCGATCACCGTCTAAAAAATGAGTGGTAAACCGACAGCCTGGCAGGTGAAGGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGtcaa                                                                              <  2:185893/249‑4 (MQ=255)                                                                                                                                                                      cgcgGCTACAACTGCGCGATCACCTTCGCAAAAATGAGTGGCAAACCGTCATCCTGGCAGGTGAAGGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGACCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTGGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGc                                                                           <  1:308432/251‑1 (MQ=255)                                                                                                                                                                      cgcgGCTACAACGTCGCGATATCCTTCCAAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCTAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACGTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGGGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGGAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTga                                                                           <  2:315493/251‑2 (MQ=255)                                                                                                                                                                           cTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑CTCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCccgc                                                                      >  1:582934/1‑251 (MQ=255)                                                                                                                                                                           cTACAACTTAGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCccgc                                                                      >  2:77563/1‑251 (MQ=255)                                                                                                                                                                             aCAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGGGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGGGCTGATCATCAGCCGCACAGTTATTGCCccgcc                                                                     <  1:469951/250‑1 (MQ=255)                                                                                                                                                                                aCTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGgtg                                                                 >  1:184095/1‑251 (MQ=255)                                                                                                                                                                                  ttCGCGATCACCTCCTCAAAAAGGGGTGGCAAACCGACTGCCGTGCAGGTGAGGGGCGAATCGCTGTGGCCTCGGGAGCCAAAG‑ACCAGCGTTTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAGGGGTGGTGGCATCCGAATCATTGGCAGAGCATGCAGGAACATCTGCGCGACGGTTCCGTAGAACAGGTCGTTGGTGAATTGCTGACCATCAGCCCCACGTTTCTTGCCCCGCCGgtgt                                                                <  2:518455/250‑1 (MQ=255)                                                                                                                                                                                   cggcgtacactttCTAAAAAAGGAGTGGCAACCCGACATCCTGGCAGGTGAATGGGAAATCGCTGTTGACCCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCCCAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCCCAGTTATTGCCCCGCCGGTGtt                                                               <  2:14818/239‑1 (MQ=255)                                                                                                                                                                                       gATCAACTTCTCAAAAATGAGAGGCAAACCAACATGCTGGCAGGTGACTGGCGATTCGCTGTTGACTTGCGAGCCAAAG‑ACCAACTTCTTCCAGGCGACGATCGACACCCACAAGCAGGAGTACGACGGGCTGTGGCAACCGAATCATTTGCAGATCCTGCAGGAACATCTGCGCTACTTTGCCGTAGAACAGAGCGACGGTGCAGTGCTGATCCTCAGCCGCACAGTTCTTGCCCCGACGGTGTTTGaat                                                          >  2:254021/1‑249 (MQ=255)                                                                                                                                                                                        aTCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGCGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGGGCTGatc                                                                                               >  2:71828/1‑213 (MQ=255)                                                                                                                                                                                        aTCACCTTCTCAAAAATGAGTGGCAAACCGACAGCCGGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGATCGATGGTGAAGTGCTGatc                                                                                               <  1:71828/213‑1 (MQ=255)                                                                                                                                                                                          cACCTTCTCAAAAATGAGAGGCACACCGACATCCTGGCAGGAGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGCACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCg                                                       >  2:245665/1‑251 (MQ=255)                                                                                                                                                                                          cACCTTCCAAAAAATGAGTGGAAAACCGACACCCGGGCAAGTGAATGGCGAATCGCTGTTGACCCGCGAGCCAAGG‑ATCAACTTCTGCAAGCCGAGGACCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTTATCATCAGCCGCACAGTTTTTGCCCCGCCGGTGTTTGACTTCg                                                       <  2:171231/251‑1 (MQ=255)                                                                                                                                                                                           cccTTCTAAAAAATGAGTGGCAAACCGACATCTGGGAAGGGGAATGGGGAACCGCTGTTGACTCGCGAGCAAAAG‑ATCAACGTCTTCAAGCCGATGATCGCAAACCCCAAGTAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCATATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCgg                                                      <  1:128096/250‑1 (MQ=255)                                                                                                                                                                                                      aaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGc                                            >  1:283935/1‑250 (MQ=255)                                                                                                                                                                                                        tGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGAGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGAGGCGCTGCAcc                                         >  1:102218/1‑251 (MQ=255)                                                                                                                                                                                                          aGTGGCAAACCGACATCCTGGCAGGTGAATGGCAAATCGCTGTTGTCTCGGTAGCCAAAG‑ATCACCTTCTTCAAGCCGATGTTCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCATATCATGCAGGAACAGCTGCGCGACTTTGCCGTAGAACATAGCGTGGGTGAGGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTa                                       <  1:339964/251‑1 (MQ=255)                                                                                                                                                                                                           gTGGCAACCCGACATCCTGGCAGGTGAAGGGCGAATTGGTTTTCCCCCCCGAGCCAAAG‑ACCAACTGCTTCAAGCCGATGATCGAAAACCACAAGCAGGAGTACGAAGGTCTGCGTCAACCGAATCATTTGCAGATCATGCAGAAACCTCTGCGCGACTTTGCCGTAGAACAGCGCGAGGGTAAAGTGCTGATCATCAGCGGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGCTGCGCTGCACCTAc                                      <  2:171788/251‑1 (MQ=255)                                                                                                                                                                                                            tGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑CTCAACTTCTTCAAGCCGATGATCGACAACCCCAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCCCAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCCCCTAc                                      >  2:269371/1‑250 (MQ=255)                                                                                                                                                                                                                   ccGACATCCTGGCAGGTGAATGGCGAACCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCGGGGGCAACCGAATCATTTGCAGATCATGCAGGAACATCGGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgcg                              <  2:166602/251‑1 (MQ=255)                                                                                                                                                                                                                     gACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgcgc                             >  2:196782/1‑250 (MQ=255)                                                                                                                                                                                                                          ccTGGCAGGAGAATGGCGAATCGCTGTTGACTCGCGAGCCAACG‑ATAAACTTCTTCAAGCCGATGATCGCCAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCt                       >  2:324588/1‑251 (MQ=255)                                                                                                                                                                                                                            tGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGACGGGCTGTGGCAACCGGATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTCTTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGc                     >  1:565030/1‑251 (MQ=255)                                                                                                                                                                                                                             ggcgggggaagggCGAATCGCTGTTGACTCGCGAGCAAAAG‑AGCAACTTCTTCAAGCCGATGATCGACACCCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGCCTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGcc                    <  2:446590/240‑1 (MQ=255)                                                                                                                                                                                                                                 ggTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACAACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGATGCCGATg                >  1:379736/1‑251 (MQ=255)                                                                                                                                                                                                                                       gggcaaacggctgttGACTCGCGAGCAAAAA‑ACCACCTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAAGCATTTGCAGATCATGCAGGAACATCTGCGCAACTTTTCCTTAGAACAGAGCGATGGTTAAGTGTTGATCATCAGCCGCACAGTTATTCCCCCGCCGGTGTTTGACTTCTGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAgg          <  1:558297/242‑1 (MQ=255)                                                                                                                                                                                                                                               cGCTGTTGACTCGCGCGC‑AAAGTATCAAATTCTTCAATCCGATGGTCGACCACCACAAGCGGGAATACGAAGGGCTGTGGAAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGCACAGAGCGATGGTGAAGTGCTGATCATCAGCCGAACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGAGGCGCTCCACCTACATCTGGCGCATCGCTGCCGTTAGCCATGTTAACGTg  >  2:50927/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       AACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAG‑ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTG  >  NC_000913/3224652‑3225140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑