Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,617	G→A	intergenic (‑28/‑77)	folB ← / → plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,617	0	G	A	100.0%	166.3 / NA	48	intergenic (‑28/‑77)	folB/plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/29);  total (19/29)

TTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGT  >  NC_000913/3204371‑3204860                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ttccccctgcgcAAAACGCCCCCCCTCGTCGTGCCCGACCCCCGTTTCCGCATTGTCAGCGTAA‑CTGTGGCAATCCGCCACCTCATCCCTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGATCACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATCTCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGAt                                                                                                                                                                                                                                                  <  2:411253/242‑1 (MQ=255)  tcccccaGCGCAAAGCGCCCCCCCCCGACTGGGCGGACCCCCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTGTACGGTTATCCCACTCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACGCCAATAGTGGTGATTACCGAAAGGTGTTCTATAAATACAATATCCATCACGTCCTTCCGGCTGTTTGGTTAACCAGATa                                                                                                                                                                                                                                                 <  2:364349/246‑1 (MQ=255)  tCCACCAGCGAAAAACCCGCCCCCCATACGTGGCTTACCCCCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAAGACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATa                                                                                                                                                                                                                                                 <  2:254152/250‑1 (MQ=255)         gcgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTcc                                                                                                                                                                                                                                         <  1:482247/251‑1 (MQ=255)                aCGCGCCCCCTCGACGTGACTGACCACCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTAATTACCGACAGTTGCTCTATAAATACAATATCCATCACGTCCTGACTGCTTTTTGGCTAACCAGATACCACTTCCGGCGaa                                                                                                                                                                                                                                   >  2:16089/1‑250 (MQ=255)                  gcgcCCCCTCGACGTGGCTGACCCCCGTTTCTGCAATGTCAGCGTAC‑CTGAGGCAATCCGCCACATCAACACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGCTCCCAGTCGTAAACCACAATAGGGGAGATTACAGAAAGTTACTCTATAAATACAATATCCATCACGACCTGCCTGCTGTTTGGCTAACCAGATACCACTTCCGGCGAAAtg                                                                                                                                                                                                                                >  1:31345/1‑251 (MQ=255)                       cccccGACGTGGCTGACCACCTTTTCTGCAATGTCAG‑GTAAACGGCGGCAATCCGCCACATCATCACTTTTCGCCTCGTTACGGGTATCCCACGACACTACGATATCGAACACTAACTTCTGTTCGATGGTCTGTGCCCAGTCGTAACCTTCAATAGTGGTGATTAGCGAAAGTTGCTTTATAAATACAATATCCATCACGTTATGCCTGCTTTTTGGATAACCACATACCGCGGCTGGCGAAATGTGCGt                                                                                                                                                                                                                           <  2:31345/247‑1 (MQ=255)                          ccgccGTGGCTGACCACCGTTTCTGCAATGTCATCTTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGtatt                                                                                                                                                                                                                        <  2:224185/247‑1 (MQ=255)                                tGGCTGACCACCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCcac                                                                                                                                                                                                                  >  2:435916/1‑251 (MQ=255)                                 ggCTGACCACCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCcac                                                                                                                                                                                                                  >  2:435839/1‑250 (MQ=255)                                     gACCACCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGAtt                                                                                                                                                                                                             >  1:512660/1‑251 (MQ=255)                                                  gCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGCAATGTGCGTATTATCCACAGATTCATCGTTGAacac                                                                                                                                                                                                >  2:161591/1‑251 (MQ=255)                                                       gTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAAtt                                                                                                                                                                                           <  1:471223/251‑1 (MQ=255)                                                         cAGCGAAA‑CTGAGGCTATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGAGGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAAtttt                                                                                                                                                                                         <  2:432347/251‑1 (MQ=255)                                                                a‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACg                                                                                                                                                                                  <  2:163196/251‑1 (MQ=255)                                                                      ggCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACa                                                                                                                                                                             <  2:322490/251‑1 (MQ=255)                                                                          aTCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCtt                                                                                                                                                                         >  1:122517/1‑251 (MQ=255)                                                                             cGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATg                                                                                                                                                                      >  2:168665/1‑251 (MQ=255)                                                                                cacaTCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGt                                                                                                                                                                   <  1:12782/251‑1 (MQ=255)                                                                                      atcaCTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACCCTCACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATc                                                                                                                                                             >  1:551233/1‑251 (MQ=255)                                                                                                gccgcTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTTCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGaa                                                                                                                                                   <  1:59056/251‑1 (MQ=255)                                                                                                  cgcTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTc                                                                                                                                                                                        >  1:218652/1‑212 (MQ=255)                                                                                                  cgcTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTc                                                                                                                                                                                        <  2:218652/212‑1 (MQ=255)                                                                                                   gcTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGa                                                                                                                                                <  1:64000/251‑1 (MQ=255)                                                                                                              aTCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATcgcg                                                                                                                                     <  2:172249/251‑1 (MQ=255)                                                                                                                      ccATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTg                                                                                                                             <  1:332767/251‑1 (MQ=255)                                                                                                                          ttCGATATAGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGAGGTGATTACCGAAAGTTGCTCTATACATACACTATCCCTCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGTCGAAATGTGCGTATTATCCACAGATTCATCGTTGAAAACGAATTTTCAAAACGGAACAGCTTATGAGAGCACTCGCGCCTGGAATGATCCTCATCGCCTACCTATGCGGc                                                                                                                         >  2:541123/1‑251 (MQ=255)                                                                                                                             gATATCGAACACTATCTTCTGGTCGATGGTCTGTTCCCAGTCGTAAACTCCAATAGTGGTGATTACCGAAAGTTGCTCGATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTGCCGGCGAAATGTGCGTATTATCCACATATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTTGAATGATCCTCATCGCGGATCTCTGCGGCTcc                                                                                                                      <  2:275549/251‑1 (MQ=255)                                                                                                                                   gAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCCTTTcc                                                                                                                >  2:420958/1‑251 (MQ=255)                                                                                                                                      cacTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGt                                                                                                             <  1:407044/251‑1 (MQ=255)                                                                                                                                       acTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTg                                                                                                            <  1:16089/251‑1 (MQ=255)                                                                                                                                                 gTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTgg                                                                                                   <  2:405025/250‑1 (MQ=255)                                                                                                                                                     gATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCt                                                                                                                                                                          >  1:508000/1‑175 (MQ=255)                                                                                                                                                     gATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCt                                                                                                                                                                          <  2:508000/175‑1 (MQ=255)                                                                                                                                                                      cGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATCCAATATACATCACGTCCTGCCTGCTTTTTGGCTAACAAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGcc                                                                             >  2:126266/1‑251 (MQ=255)                                                                                                                                                                         aaaCACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATGCAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGGGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCg                                                                           <  2:138967/250‑1 (MQ=255)                                                                                                                                                                            cacCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATcc                                                                       >  1:380777/1‑251 (MQ=255)                                                                                                                                                                                               cGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGc                                                    >  1:204350/1‑251 (MQ=255)                                                                                                                                                                                                   aGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATc                                                <  2:122517/251‑1 (MQ=255)                                                                                                                                                                                                                       atCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTAATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGtt                             >  2:280501/1‑250 (MQ=255)                                                                                                                                                                                                                       atCCATCACGTCCTGCCTGCTTTGTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGtt                             <  1:443848/250‑1 (MQ=255)                                                                                                                                                                                                                        tCCATAACGTCCTGCCTGCTTTTTGGCTACCCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGGGTTAc                           >  2:466174/1‑251 (MQ=255)                                                                                                                                                                                                                                gTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCggg                   >  2:199143/1‑250 (MQ=255)                                                                                                                                                                                                                                gTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGt                   <  1:541123/251‑1 (MQ=255)                                                                                                                                                                                                                                  cctgcctgCTTTGTGGCTAACCAGATTCCACTTCCGTCGAAATGTTCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTAGGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCTGATCCGCGAACCTGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTgg                 <  2:512660/251‑1 (MQ=255)                                                                                                                                                                                                                                         gcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGGGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGTTGGCAAGGGa          <  1:92390/251‑1 (MQ=255)                                                                                                                                                                                                                                            ttttGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGag         <  1:435261/249‑1 (MQ=255)                                                                                                                                                                                                                                                 gCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGt  <  1:154457/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    TTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAA‑CTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGT  >  NC_000913/3204371‑3204860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑