| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 292,433 | G→A | F172F (TTC→TTT) | yagK ← | uncharacterized protein YagK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 292,433 | 0 | G | A | 98.2% | 183.6 / ‑6.4 | 57 | F172F (TTC→TTT) | yagK | uncharacterized protein YagK |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (28/27); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCA > NC_000913/292190‑292678 | gAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAg < 2:367936/251‑1 (MQ=255) aCGTTCTTTAGCAGCCGCCTTGCTGATCACTATCAGTCATCTATAGACAATTGCGTAACGACACTGCATTTTAGTAATTTTTTGACACAATACAACTAAGGCCTTCTCACTGCGAAGGCCATTACAGCCTTACAGTTAGATTTGGCTACAGCCGAAGTTACGGTCTCATTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAATTCAGTAc > 2:484022/1‑251 (MQ=255) ttAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGgt > 2:574395/1‑251 (MQ=255) gtagCGGACTTGCTGATGACTTTCATTAATCTATAGAAAATTGCGTAACGAAACTTCATTGTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACTCTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGgtgcc < 2:432004/251‑3 (MQ=255) tgaCTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTGCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTGTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTGCTGCTGAAAGTc < 2:290045/220‑1 (MQ=255) tgaCTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTCAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTc > 1:290045/1‑220 (MQ=255) gTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGCCCTTCTCACTGAGAAGGCCCTTACCGCCTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGACTCCTTCACCACACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGCACGTCAGTACTATTGGTGCCTAGCACCTACTTACAGTTTTCAgg > 1:586447/1‑250 (MQ=255) ataGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAAt < 2:447030/248‑1 (MQ=255) ataGAAAATTGCGTAAAGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTGCAGGAAAATg < 2:366909/249‑1 (MQ=255) aGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTTATGTAGTTCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTTAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTCCAGGAAAATGgac < 2:309456/250‑1 (MQ=255) aaaTTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGgaccaga < 1:153843/251‑1 (MQ=255) aCTGCATTTTAGTAAGTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACGTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGtct < 2:434572/251‑1 (MQ=255) actggtAGTAATTGTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGTACATTACCGAATTACAGTTCGATTTGGCTACAGCAGAAGTTACGGTCTCCTTCCCCAAACACTTTGCTTTCAACTTGGGTCAGGTATTCCAGTCGGGTTAACAGCGCCGGGTAGTACTGCTGAAAGTCAGTCCTCTTGGTGTCTAGCACAGTCTTACCGTTTTACTGAAAATGGaccagaccagcttgatcttgtctct > 2:172578/6‑236 (MQ=255) aGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGtc > 1:251612/1‑251 (MQ=255) gTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGtct > 1:320837/1‑251 (MQ=255) tttttGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTCGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAggg > 2:417090/1‑251 (MQ=255) gAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTg > 1:379424/1‑251 (MQ=255) aaCAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGt > 1:54910/1‑250 (MQ=255) aCAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGCTTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTAc > 2:343481/1‑251 (MQ=255) aaTACAAATAAGTCCTTCTCACTGAGAAGGCCATTACCGACTTCCAGTTCGAATTGGCTACAGCCGAAGTTACGGTCTACTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTGAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTTGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCGGGGTGATCGTCTCGCTCCAGTCTCAGGGTGCTGTACCa < 2:404509/251‑1 (MQ=255) aaTACAAATAAGGCCTTCTCACGGAGAAGGCCATTACCGTCTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGTGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCa < 2:404550/251‑1 (MQ=255) aTACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTAcc > 2:411660/1‑249 (MQ=255) tACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGACGCTGTACCAgg > 2:123625/1‑251 (MQ=255) aaTAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTcc < 1:411660/251‑1 (MQ=255) aaGGCCTTCTCACTGAGAAGGACATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCtgt > 2:107512/1‑251 (MQ=255) aGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCtgtg > 1:257463/1‑251 (MQ=255) aGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGGGATCGTCTCTCTCCCGTCTCAGGGCGCTGTACCAGGCTCCtgtg > 2:399974/1‑251 (MQ=255) gaAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAgg > 2:333903/1‑251 (MQ=255) aGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGATCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTACGGtt > 1:235565/1‑251 (MQ=255) gCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTAGTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTAt < 2:136972/251‑1 (MQ=255) ttACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTc > 1:218283/1‑251 (MQ=255) tACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTcc > 1:140822/1‑251 (MQ=255) tACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTc < 2:169493/250‑1 (MQ=255) aGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATc > 1:427578/1‑251 (MQ=255) aGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGGCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGGCCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATc < 1:70626/251‑1 (MQ=255) gCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggtggt < 1:343481/251‑1 (MQ=255) gTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCtt > 1:69862/1‑251 (MQ=255) gTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGGGGTGGTAGTAAGCGTCTttgttg > 2:126702/1‑250 (MQ=255) ttCACCACACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaacaac > 1:378849/1‑250 (MQ=255) tCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaacaac < 1:482913/249‑1 (MQ=255) aCCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGc < 1:107512/251‑1 (MQ=255) aaaCACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGCTCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGAAGTAAGCGTCTTTGTTGAACAACAGGCaa > 2:209721/1‑250 (MQ=255) cacTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAAtgt < 1:333903/251‑1 (MQ=255) tttGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAgg < 2:99044/241‑1 (MQ=255) tttGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAgg > 1:99044/1‑241 (MQ=255) ttGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGAt < 2:54910/250‑1 (MQ=255) tGCTTTCAACTTTGGTCAGGAAGTCCAGTCGGGATAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGCCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTAAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCaactgggat > 2:379337/1‑242 (MQ=255) cAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGGGATCGTCTCTCTCCAGTCTCAGGGCTCTGTACCAGGCTCCTGAGATCATCCCTCTAAGGTTATCGTTCTGATCGTAATCCCCGAGGAGGTAGTAAGCGTCTTTGTTGAACAACAGCCAAAGGGGATAATGGCAt > 1:444696/1‑251 (MQ=255) tGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCttt < 2:318504/214‑1 (MQ=255) tGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCttt > 1:318504/1‑214 (MQ=255) ggTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGGTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTTATAGTGGCATTTACCTGATTCa < 1:370562/251‑1 (MQ=255) ggTAGTCCAGTCGGGGTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACGATTGGTGGCGAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTGTCTCTCCAGTCGCAGGGCGCTGTGCCAGGCTCCTGGGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTTGTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGAtgca < 2:384186/251‑4 (MQ=255) aGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGaa < 2:462976/251‑1 (MQ=255) gcCTGGTAGTTCTGCTGAAAGGTAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCGCTCCAGTCTCAGGGCGCGGTTCCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTTTTTGTTGAACAACAGGCAAAGGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGTCCATataat < 2:586347/251‑1 (MQ=255) ggTAGTTCTGCTGAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATaataaat < 1:18037/251‑1 (MQ=255) gctgAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAggg < 1:574395/248‑1 (MQ=255) gctgAAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCa > 1:121841/1‑250 (MQ=255) | GAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCA > NC_000913/292190‑292678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |