Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,150,316	G→A	E219K (GAG→AAG)	yghA →	NADP(+)‑dependent aldehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,150,316	0	G	A	100.0%	242.2 / NA	75	E219K (GAG→AAG)	yghA	NADP(+)‑dependent aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/38);  total (37/38)

GGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGT  >  NC_000913/3150078‑3150559                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    ggTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGAGTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGATGAAAGGTATTc                                                                                                                                                                                                                                         <  2:245343/251‑1 (MQ=255)    tGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAACGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGCCTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACCGGTCGCGAGGAAAGGGATTCggg                                                                                                                                                                                                                                      >  2:13211/1‑251 (MQ=255)       cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCACCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCCTTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGAATTCGGGTg                                                                                                                                                                                                                                    >  1:532865/1‑250 (MQ=255)          tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAat                                                                                                                                                                                                                                 >  1:445922/1‑250 (MQ=255)          gccGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCGAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGAGTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAat                                                                                                                                                                                                                                 <  2:68954/249‑1 (MQ=255)           ccGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCCTTAACGTTTTCGCGCTGTTCTGGATAACCCAGGAAGCGATCCCACTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACAAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAAATACAGCCGAGGCTTGGCAAACCAGGTCGCGAAGAAAGGGATTCGGGAGAatat                                                                                                                                                                                                                               >  2:413143/1‑251 (MQ=255)             ggATATTGCAGACCTCACCAGCGAACCGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTg                                                                                                                                                                                                                             >  2:388560/1‑251 (MQ=255)             ggATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTg                                                                                                                                                                                                                             >  1:168264/1‑251 (MQ=255)               atatTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTc                                                                                                                                                                                                                           >  1:188870/1‑251 (MQ=255)                    gCAGCCCTCACCAGCGAACAGTTTCAAAAGACCGTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGcc                                                                                                                                                                                                                      <  2:474494/251‑1 (MQ=255)                    gCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGcc                                                                                                                                                                                                                      <  1:195363/251‑1 (MQ=255)                             aCCAGCGAACAGTTTAAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGACAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAACGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGAt                                                                                                                                                                                                             >  2:297668/1‑251 (MQ=255)                                     aCAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTACCCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGAc                                                                                                                                                                                                       >  1:342739/1‑249 (MQ=255)                                     aCAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACGTCGTCAAGCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAg                                                                                                                                                                                                     <  2:222524/251‑1 (MQ=255)                                       aGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGc                                                                                                                                                                                                    >  1:474576/1‑250 (MQ=255)                                           tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGc                                                                                                                                                                                               >  2:142685/1‑251 (MQ=255)                                             aaaaGACCTTTGCCATTATCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATAATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTACGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCa                                                                                                                                                                                              >  1:361972/1‑250 (MQ=255)                                              aaaGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCaa                                                                                                                                                                                             >  2:549807/1‑250 (MQ=255)                                                   ccTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTTGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGATAGGTATTCGGGGGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTcc                                                                                                                                                                                       <  2:532865/251‑1 (MQ=255)                                                      gtGCCATTAACGTTGTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCTCTTCGGCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCcgg                                                                                                                                                                                     <  2:573239/249‑1 (MQ=255)                                                       tGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCcggcg                                                                                                                                                                                   >  2:445367/1‑251 (MQ=255)                                                        gCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCcggcgg                                                                                                                                                                                  <  2:62306/251‑1 (MQ=255)                                                               aCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACg                                                                                                                                                                           >  2:489776/1‑251 (MQ=255)                                                                            ggtCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGAt                                                                                                                                                                <  1:269431/247‑1 (MQ=255)                                                                                tGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGc                                                                                                                                                           >  2:558189/1‑250 (MQ=255)                                                                                 ggCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAAc                                                                                                                                                                            <  2:50347/232‑1 (MQ=255)                                                                                 ggCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAAc                                                                                                                                                                            >  1:50347/1‑232 (MQ=255)                                                                                  gCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTAACATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACGATGCGGCTACGAAGGCGTCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATGTCCGGCGGACAAACGCAGGATAAGATCCCGCAGt                                                                                                                                                        <  2:517858/251‑1 (MQ=255)                                                                                        ccAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTGCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTc                                                                                                                                                  <  2:120646/251‑1 (MQ=255)                                                                                           ggAAGCGATCCCCCTGCTACCGAAAGGTGCAAGGATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACGACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGc                                                                                                                                               <  2:484066/251‑1 (MQ=255)                                                                                            gAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGCTCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCCGGATAAGATCCCGCAGTTTGGTCAGc                                                                                                                                               >  2:444991/1‑250 (MQ=255)                                                                                                 gatcccaatGCTACCGAAAGGTGCAAGTCTCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGACGCGAGGGAAGGTATTCGGGAGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGCCAAACTCAGGACAAGATCCCGCAGTTTGGTCAGCCAACGc                                                                                                                                         >  2:388644/9‑251 (MQ=255)                                                                                                 gATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGc                                                                                                                                         >  1:62325/1‑251 (MQ=255)                                                                                                    cccccTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCg                                                                                                                                       <  2:198979/250‑1 (MQ=255)                                                                                                      cccTGCTACCTAAAGGTGCAATTATCATCACCACTTCGTCAATCCAGGCATTCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGAta                                                                                                                                    <  2:8205/251‑2 (MQ=255)                                                                                                                gAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCgg                                                                                                                          <  1:549807/251‑1 (MQ=255)                                                                                                                 aaaGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCggg                                                                                                                         >  1:251417/1‑251 (MQ=255)                                                                                                                          aGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGACCTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGGCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGTCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTAAGCAAACGCCGGTGCAACGTGAGGGGCAACcggc                                                                                                                >  2:210438/1‑251 (MQ=255)                                                                                                                               catcaCCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGaa                                                                                                            <  1:46880/250‑1 (MQ=255)                                                                                                                               catcaCCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGaa                                                                                                            <  1:445367/250‑1 (MQ=255)                                                                                                                                    ccacTTCGTCAAGCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTGTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGTCGGAACTGGcc                                                                                                      <  2:240582/251‑1 (MQ=255)                                                                                                                                          cGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGt                                                                                                 <  1:444991/250‑1 (MQ=255)                                                                                                                                              aaTCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGCGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCCGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAAATGGCCCCTGTATATg                                                                                            >  2:483811/1‑251 (MQ=255)                                                                                                                                                 ccAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGttt                                                                                         >  1:454586/1‑251 (MQ=255)                                                                                                                                                   aGGCATGCCAGCCAATTCCGCATTTACTTGACTATTCGGCGACGAAGGCGGCGATTCTGAACTCCAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATAGGGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCGGTGTGGTCAGCAAACGCCGATGAAACGTGCTGGGCAACCCGCGTAACTGGTTGCTGGATATGTTTAt                                                                                       <  2:361972/251‑1 (MQ=255)                                                                                                                                                      cATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTg                                                                                    <  1:80100/251‑1 (MQ=255)                                                                                                                                                       aTACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTgg                                                                                   <  2:144875/251‑1 (MQ=255)                                                                                                                                                       aTACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTgg                                                                                   >  1:325459/1‑251 (MQ=255)                                                                                                                                                             gCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTc                                                                             <  2:342739/251‑1 (MQ=255)                                                                                                                                                                  gTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGGTGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAgg                                                                          >  1:405234/1‑249 (MQ=255)                                                                                                                                                                     cGCATTTACTGGACTATGCGGCGACTATGGCGGCGATTCTGAACTACAGCCGTGTCTTGGCTAAACAGTTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAATCGCAGGATAAGATCCCGCTGTTTGGTCAGCAAACGCCGATTAAACGTGCGGGTCAAGCGGCGTAACTGGCCCCTGTATAGGTGTATCTGGCAAGTCAGTAGTCg                                                                     <  2:352391/251‑1 (MQ=255)                                                                                                                                                                       cATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCACGTCAGGAGTCg                                                                     >  1:199369/1‑249 (MQ=255)                                                                                                                                                                       cATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAg                                                                   <  1:90139/251‑1 (MQ=255)                                                                                                                                                                            aCTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAACCGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGCGCCGAGCTACg                                                              >  2:517462/1‑251 (MQ=255)                                                                                                                                                                              tGGACTATTCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGGCGCGAAGAAAGGAATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCCAAATTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTAGTCCGCAAACGACGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCACTGAATATGTTTATCTGGCAAGGCAGGAGTCGAGCTACGTc                                                            >  2:479289/1‑251 (MQ=255)                                                                                                                                                                               ggACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACg                                                              <  2:199369/248‑1 (MQ=255)                                                                                                                                                                                 aCTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCAcc                                                         <  1:297668/251‑1 (MQ=255)                                                                                                                                                                                 aCTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCAcc                                                         <  1:575926/251‑1 (MQ=255)                                                                                                                                                                                        ggCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGa                                                    >  1:228699/1‑249 (MQ=255)                                                                                                                                                                                        ggCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGa                                                    >  1:279989/1‑249 (MQ=255)                                                                                                                                                                                        ggCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAg                                                  <  2:437854/251‑1 (MQ=255)                                                                                                                                                                                         gCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGt                                                 <  1:558189/251‑1 (MQ=255)                                                                                                                                                                                                  gcggcgATGCGGAACGCCAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATGCGGGTGAATATTGTCGCGCCAGGCCCTATCGGGACAGCACTGCAAATTGCCGGCGGACAAACGCAGGATAAGAGCCCGGAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGTCGTAACTGGCCCCTGTATATGTGTATCTGGGAAGTCAGTAGTCGAGCTACGTCACCGCAGAAGTGCACGGCgt                                        <  1:522676/251‑1 (MQ=255)                                                                                                                                                                                                    ggcgATTCTGAACTACAGCCGAGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGACGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGACACCGCAGAAGAGCACGGCgtgt                                      >  1:393121/1‑251 (MQ=255)                                                                                                                                                                                                      cgATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTgc                                    >  1:166081/1‑251 (MQ=255)                                                                                                                                                                                                      cgATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTgc                                    >  1:242291/1‑251 (MQ=255)                                                                                                                                                                                                             gAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGTATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTgcggcggcg                             <  1:388644/251‑1 (MQ=255)                                                                                                                                                                                                                atACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGc                          <  2:168264/250‑1 (MQ=255)                                                                                                                                                                                                                  aCAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGTGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATTTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCa                         <  1:413143/250‑1 (MQ=255)                                                                                                                                                                                                                         tGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGt                                                                                                 <  2:490606/171‑1 (MQ=255)                                                                                                                                                                                                                         tGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGt                                                                                                 >  1:490606/1‑171 (MQ=255)                                                                                                                                                                                                                             ttGGCACAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCCCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGCCAAACGCAGGCTAAGATCCCGCAGTTTGGTCAGCAAAAGCCGATGAACCGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGCTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCAAGGCGTGTGCGGCGGCGAGCATTTAGGTTaaa              >  2:278035/1‑250 (MQ=255)                                                                                                                                                                                                                                    aaCAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATATGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGATTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGCGCATTTAGGTTAAAAAAATGcc      >  1:219528/1‑251 (MQ=255)                                                                                                                                                                                                                                      cAGGTCGCGAATAAAGGTAGTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCTGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCATCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAATAAAATGCCCg    <  2:448315/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGAAGAAGTGCACGGCGTGTGCGTCGGCGAGCATTTAGGTTAAAAAAATGCCCGGt  <  1:186322/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    GGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGT  >  NC_000913/3150078‑3150559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑