Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,145,944	G→A	L106L (CTC→CTT)	hybO ←	hydrogenase 2 small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,145,944	0	G	A	100.0%	151.5 / NA	45	L106L (CTC→CTT)	hybO	hydrogenase 2 small subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/25);  total (20/25)

AATATTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTA  >  NC_000913/3145698‑3146188                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     aaTATTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAagag                                                                                                                                                                                                                                                  <  1:31164/251‑1 (MQ=255)        aTAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTAtg                                                                                                                                                                                                                                           <  2:199273/251‑1 (MQ=255)              gTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTAtgtttgtt                                                                                                                                                                                                                                     >  1:176777/1‑251 (MQ=255)                tttGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTtc                                                                                                                                                                                                                                    <  2:197890/250‑1 (MQ=255)                   gccgggCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCtt                                                                                                                                                                                                                                <  2:367422/247‑1 (MQ=255)                   gCCTGGCAGAACTTCGTGCAGGCTGACGGCGCCAGTTGGGGTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGAGGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTATCACATACTGCCCTTTGTACTTCTCAAGAGCGTTATTTTTGTTctct                                                                                                                                                                                                                                 <  1:371710/250‑1 (MQ=255)                              cttcttGAAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGGTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCtgatg                                                                                                                                                                                                                      >  2:356161/1‑250 (MQ=255)                                   tGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGa                                                                                                                                                                                                                 >  1:395440/1‑250 (MQ=255)                                      aGGCTGACTGCGCCAGGTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCGCTTCGACCTGATGACCGAAggc                                                                                                                                                                                                             <  2:514317/251‑1 (MQ=255)                                       ggCTGACTGCGCCAGTTGGGTTAACTCCCGCTGCGGCAACACCGCCCCACGCAGAGCAGGCACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcg                                                                                                                                                                                                            >  2:202546/1‑251 (MQ=255)                                          tGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGgcggcg                                                                                                                                                                                                         >  1:386249/1‑251 (MQ=255)                                           gACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcggc                                                                                                                                                                                                          <  1:385062/249‑1 (MQ=255)                                                     gTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTc                                                                                                                                                                                              <  2:291038/251‑1 (MQ=255)                                                          gTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcgg                                                                                                                                                                                                           <  1:268601/233‑1 (MQ=255)                                                          gTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcgg                                                                                                                                                                                                           >  2:268601/1‑233 (MQ=255)                                                                  cAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCagg                                                                                                                                                                                 >  2:50475/1‑250 (MQ=255)                                                                           cAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtc                                                                                                                                                                        <  1:433993/251‑1 (MQ=255)                                                                                 cGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGt                                                                                                                                                                  <  2:316934/251‑1 (MQ=255)                                                                                     ccACGCAGAGCCGGAACCGATAGCGAGAATGGCTGCTGAGCCTTCAGCCGCTTTGCGGAAATGCTACACAATCGGAT‑ACACGGCAATCATGCACTCAATACCGTTATATTTTAATGGGATGGAACCAACCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCCTCGACCTGATGACCGAAGGCGGCGGACAGCACTTCGTGGTATTCCAGAGAGATCGTCTCCAGTACg                                                                                                                                                               >  2:540882/1‑250 (MQ=255)                                                                                             agCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCGTTGCTGATGTGATCCACAATCGGCTTAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACGAACACATACTGCCCTTTGTACTTCTCAAGGGCGTTATGTTTGTTCTCTTCGGCGTGAGGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCt                                                                                                                                                      <  1:540882/251‑1 (MQ=255)                                                                                              gCAGGAACCGATAGCGAGAATGGCTGCTGCGCCTTCCGCCGCTTTGCTGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATTGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTa                                                                                                                                                     <  1:384442/251‑1 (MQ=255)                                                                                                           gCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGt                                                                                                                                        >  2:130666/1‑251 (MQ=255)                                                                                                                 aTGGCTGCTGCGCCTGCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACGAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTGTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACGCCAGAGAGATAGTCGCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACg                                                                                                                                  <  2:332280/251‑1 (MQ=255)                                                                                                                       gctgcGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCag                                                                                                                            <  1:124142/251‑1 (MQ=255)                                                                                                                          gcgcCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCGCTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCagag                                                                                                                          <  1:299241/250‑1 (MQ=255)                                                                                                                                   gccgcTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCCACCATGCACTCACTACCGATATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTAATTCTCAAGAGCGTTATGTTTGTTCTCTTCGACATGATGCCCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATCGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTAc                                                                                                                 >  2:92915/1‑250 (MQ=255)                                                                                                                                    ccgcTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGCTTCCGTACa                                                                                                                >  2:184085/1‑250 (MQ=255)                                                                                                                                               aTGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTc                                                                                                    <  1:320741/251‑1 (MQ=255)                                                                                                                                                   gATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTAGCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGAACGAAGCAGAGATTCCGTACAACCGGTGCACTcccgc                                                                                                <  2:123974/251‑4 (MQ=255)                                                                                                                                                          aaTCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTCATGGGATGGAACACTCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGACGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCAAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCa                                                                                          >  2:342683/1‑250 (MQ=255)                                                                                                                                                                        gCAACCATGCCATAACTACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTgg                                                                            >  2:380004/1‑251 (MQ=255)                                                                                                                                                                             cATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGAc                                                                       <  1:39587/251‑1 (MQ=255)                                                                                                                                                                                 cAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTg                                                                   <  2:15489/251‑1 (MQ=255)                                                                                                                                                                                   ataaataCCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCg                                                                 >  2:508845/1‑251 (MQ=255)                                                                                                                                                                                     aaataCCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCggg                                                               >  2:145868/1‑251 (MQ=255)                                                                                                                                                                                      aataCCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGt                                                              <  2:386249/251‑1 (MQ=255)                                                                                                                                                                                             ttATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAAc                                                       >  1:256680/1‑251 (MQ=255)                                                                                                                                                                                               aTCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAAcc                                                      <  2:209555/250‑1 (MQ=255)                                                                                                                                                                                                   tttAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGAtt                                                  <  1:202546/250‑1 (MQ=255)                                                                                                                                                                                                       aTGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCCCAAGCGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGACGGACGCTGCGGGTTAGTAACCGATTCGGcc                                             >  1:37863/1‑251 (MQ=255)                                                                                                                                                                                                                                cacaTACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACtt                     >  2:6654/1‑250 (MQ=255)                                                                                                                                                                                                                                         cccTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGAGAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCAtgg           <  1:244260/251‑1 (MQ=255)                                                                                                                                                                                                                                              tGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCGACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTggc       <  1:586658/250‑1 (MQ=255)                                                                                                                                                                                                                                                tACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTggcggc    >  1:322280/1‑251 (MQ=255)                                                                                                                                                                                                                                                   ttCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGCGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTa  >  1:563114/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     AATATTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCAC‑CGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTA  >  NC_000913/3145698‑3146188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑