Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,120,786	G→A	T165I (ACT→ATT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,120,786	0	G	A	100.0%	256.6 / NA	78	T165I (ACT→ATT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/38);  total (40/38)

CGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGA  >  NC_000913/3120539‑3121026                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 cGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAatat                                                                                                                                                                                                                                               <  1:59899/251‑1 (MQ=255)       aaTATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGACTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCCCAAGAACCGACAGGA‑ACGTTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCCGACCTGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCa                                                                                                                                                                                                                                          >  2:403434/1‑250 (MQ=255)          ataGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCTCGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATc                                                                                                                                                                                                                                       >  2:298224/1‑250 (MQ=255)          ataGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATc                                                                                                                                                                                                                                       >  1:445816/1‑250 (MQ=255)          ataGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCGTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGTAACGGATCGATTCCCGGTACCTGACCGGCGACCAGAAGCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCa                                                                                                                                                                                                                                      <  1:298224/251‑1 (MQ=255)             gTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACACGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCAACGGCGCCATATTGGCAATCAgcc                                                                                                                                                                                                                                   >  1:237687/1‑249 (MQ=255)                    gTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGccc                                                                                                                                                                                                                            <  1:445891/251‑1 (MQ=255)                           aCTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGCCCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATAAGACACAGCCCCGCcgcg                                                                                                                                                                                                                     >  2:1033/1‑251 (MQ=255)                             tGAGTGACAGCGTAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCCCAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCACACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGt                                                                                                                                                                                                                    >  1:582492/1‑250 (MQ=255)                                 tGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAAAGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAAc                                                                                                                                                                                                               >  1:135736/1‑251 (MQ=255)                                 ggACAGCGAAGCTTCCCCCAGCAACCGGCGCCGCTGGCCACGTCTCGTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTTACCGGCGACCAGAATCGGCACGCCCAACGCACCAAAGGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAAc                                                                                                                                                                                                               <  1:544740/250‑1 (MQ=255)                                  gACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGCCCACGTCTCTTTCCCCCCTTCCCAGCCTTCCAGCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACGGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCTCGTATAACg                                                                                                                                                                                                              <  2:93258/251‑1 (MQ=255)                                        gAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTg                                                                                                                                                                                                         >  1:150875/1‑250 (MQ=255)                                        gAAGCTTCCCCCAGCAACCAGAGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGCCCAGAATCGGCACGCCCAACGCACCAAAAGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTCTAACGGTTTg                                                                                                                                                                                                         >  2:246033/1‑250 (MQ=255)                                              tCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGccc                                                                                                                                                                                                   <  1:196250/250‑1 (MQ=255)                                              tCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGccc                                                                                                                                                                                                   >  1:97408/1‑250 (MQ=255)                                                 cccAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGc                                                                                                                                                                                               >  1:368425/1‑251 (MQ=255)                                                        cccAGCGCCGCTGGCCACGTCTCTTTCACCCCTGTCAAGCCGTCCACCATTGCTACCAGCCATACCGGCAAAAGAACCGACAGGA‑ACGATAGCTGACGTCCCGCCATTGCGCCAAGGTGGAACGGATCGATGCCCGTTACCGTACCGGCGACCAAAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATGGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACcag                                                                                                                                                                                        <  2:54017/250‑1 (MQ=255)                                                              gccgcTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAgcgc                                                                                                                                                                                  >  1:153880/1‑251 (MQ=255)                                                                cgcTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAg                                                                                                                                                                                                                                     >  1:331123/1‑198 (MQ=255)                                                                cgcTGGCCACGCCCCTTTCCCCCCTTGCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAg                                                                                                                                                                                                                                     <  2:331123/198‑1 (MQ=255)                                                                     cccACGTCTTTTTCCCCCCTTTCCAGCCGTCCATCTGTGCTACCAGCCAGACCGGCACAAGACCTGACAGGA‑ACGGTAACTGACGTCCCGCCAGTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTa                                                                                                                                                                           <  2:209619/250‑1 (MQ=255)                                                                            ctctTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGcc                                                                                                                                                                     >  1:513921/1‑250 (MQ=255)                                                                                ttcgccccTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCACACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATACCGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCgg                                                                                                                                                                >  2:11309/5‑251 (MQ=255)                                                                                ttccccccTTTCCAGCCGTCCATCATTTCTACCAGCCAGACCGCCCCAAGAACCTACAGGA‑TCGGTACCTGCCGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGCCCATAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGTGCACCGGTAATCGCCACCg                                                                                                                                                                 <  2:358164/246‑1 (MQ=255)                                                                                ccccccccttcacAGCCGCCCATCTTTGCTCACAACTAGAACGCCCCAAGAACCGACAGTA‑ACGGCAACTGACGTCCCGCCATTGCTCCAATGTGGAACGGATCGATTCCCGTTTCCCGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCTACCGGCTCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGGCCACCATCAGCGAACCGGTAATCGCCAcca                                                                                                                                                                 <  2:360484/238‑2 (MQ=255)                                                                                    ccccTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGgcgc                                                                                                                                                             >  1:529346/1‑250 (MQ=255)                                                                                     cccTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACcagc                                                                                                                                                                                       <  1:557892/223‑1 (MQ=255)                                                                                     cccTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCAc                                                                                                                                                           >  2:474122/1‑251 (MQ=255)                                                                                     cccTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGATTGAAGCCCAGGCCCACcagc                                                                                                                                                                                       >  2:557892/1‑223 (MQ=255)                                                                                      ccTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCAcc                                                                                                                                                          >  1:311825/1‑251 (MQ=255)                                                                                           ccAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagc                                                                                                                                                     >  1:100476/1‑251 (MQ=255)                                                                                                      tcatTCCTACCAGGCAGAACGGCACAAGGACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAAAGGATCGATTCCCGTTAAATGACCGGCGACCAGAATCGGCACGCCCAATGCACCAAACGCCACCGGCGCAATATAGGCAATCAGACACAGCCCCGCCGCGTATAACGGATTGACGCACAGGCCCACCAGCATCGCACCGGTAATCGCCACCGGCGCACCAACGCCAGCCGccccc                                                                                                                                          >  2:518811/1‑247 (MQ=255)                                                                                                       catTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCt                                                                                                                                          <  1:509396/250‑1 (MQ=255)                                                                                                       catTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGCCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCt                                                                                                                                          <  1:1033/250‑1 (MQ=255)                                                                                                           gcgcccagccagACCGGCTCAAGACCCGACAGAA‑ACGTTACCTGACTCCCCGCCATTGCGCCAATGGGGAACGGATCGATGCCCGTGACCTGACCGGCGACCAGAATCGGCACGCCCAACGCGCCAAACGCCCCCGGCGCAATATTGGCAATCAGACACAGCCCCTCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGTTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCa                                                                                                                                      <  2:42348/246‑1 (MQ=255)                                                                                                            cTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGc                                                                                                                                    >  1:393776/1‑251 (MQ=255)                                                                                                                  gccagAACGCCACAAGAACCGAAAGGA‑CCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCTCCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCa                                                                                                                              <  1:57461/251‑1 (MQ=255)                                                                                                                    cagAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCAACCGGCGCACCAAAGCCAGCAGCTCCTTCCAACAAAGCAcc                                                                                                                            >  1:521268/1‑251 (MQ=255)                                                                                                                     agAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGCCCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCAcc                                                                                                                            >  1:337985/1‑250 (MQ=255)                                                                                                                        ccGGCACAAGAACCGAC‑GGACACCGTACCTGGCTCCCCGCCATTGCGCCAGTGTGCAACGGACCTACTCCCGTTACCTGACCGGCGACCTGAAGCCGCACGCCCACCGCCCCAAACGCCCCCGGCGCAATATTGGCATGCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGGAATCGCCACCGGCGCCCCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAg                                                                                                                        <  2:338264/250‑1 (MQ=255)                                                                                                                           gcaaaaGAACGGCCAGGA‑ACGGTAACGGCCCTCCCGCCATTCCGCCAATGGGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAAGCGGCACGCCCAACGCACCAAACGCCACCGGCGCAAGATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGgag                                                                                                                     <  1:105217/247‑1 (MQ=255)                                                                                                                            cacaAGAACCGACAGAA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGGTTGAAGCCCAGGCCCTCCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGgaga                                                                                                                    <  1:278265/251‑1 (MQ=255)                                                                                                                                aGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGAACCAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAAcc                                                                                                                >  2:581460/1‑251 (MQ=255)                                                                                                                                   aCCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAAt                                                                                                             >  1:2150/1‑251 (MQ=255)                                                                                                                                    ccgccAGGA‑CCGTTAACTGACGCCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCACCGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATc                                                                                                            <  2:445816/247‑1 (MQ=255)                                                                                                                                       aCAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGt                                                                                                         <  1:101186/251‑1 (MQ=255)                                                                                                                                            a‑aCGGGAACTCACGCCCCCCCATGGCGCCAATGTGGAACGGATCGATTCCCTTTACCTGACCGGCAACCAGAACCGGCACGCCCAACGCCCCAAACGCCACCTGCGCAATATGGGCAATCAGACACACCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGATAAACCAATCAGTAacac                                                                                                    <  2:197044/251‑1 (MQ=255)                                                                                                                                                 gtacctgccgccccGCCATGGCGCCAATGTGGAACGGAACGATTCCCGTTCCCTGACCGGCGACCAGAACCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGc                                                                                                <  2:166756/240‑1 (MQ=255)                                                                                                                                                          gTCCCGCCATTGCGCCACTGTGGCACGGCTCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGACCCGCCGCGTATAGCGGTTTGAAGCCCAGGCCCACCAGCAGCGCCCCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCCGCAACGCACCAAAGGAGAAACCAATCAGTAACCCCTGCAAACGCta                                                                                        >  2:43869/1‑249 (MQ=255)                                                                                                                                                           ccccGCCCTTGCGCCAATGCGGAACGTACCGATCCCCGTTACCTGTCTGGCCACCAGACGCGGCCCGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGCCACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCCCCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTgct                                                                                      <  2:45869/250‑3 (MQ=255)                                                                                                                                                             ccGCCATTGCGCCAATGTGGACCGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcg                                                                                    >  1:450814/1‑251 (MQ=255)                                                                                                                                                                ccATTGCGCCAATGTGGCACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATAGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATAAGACACAGGCCCGCCGCGTATAACGGTTTGAAGCACAGGACAACCAGCAGCGCACCGGTAATAGCCACAGGCGCACCAAAGCCAGCAGCTCCTTCCAGCAAAGCACCAACGGAGAAACAATTCAGTAACAACTGCAAACGCTGAtcgtcg                                                                                 >  2:207235/1‑251 (MQ=255)                                                                                                                                                                ccATTGCGCCAATGTGGAACGGATCGATTCCCTTTACCTGACCGGCGACCAGAATCGGACCGCCCAACGCCCCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGTCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcgtcg                                                                                 <  2:359912/251‑1 (MQ=255)                                                                                                                                                                     gcgcCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGa                                                                             <  1:100808/250‑1 (MQ=255)                                                                                                                                                                      cgcCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATg                                                                           >  1:18073/1‑251 (MQ=255)                                                                                                                                                                      cgcCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATg                                                                           <  1:454857/251‑1 (MQ=255)                                                                                                                                                                        ccAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGga                                                                         <  2:18073/251‑1 (MQ=255)                                                                                                                                                                         cAATGTGGAACGGATCGAGTCCCGTTACCTGACCGGCGACCAAAAGCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACCCAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCGCCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGgag                                                                        <  2:135736/251‑1 (MQ=255)                                                                                                                                                                                gAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCCCGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCTTCGGTGATGGAGATAACCg                                                                 <  1:438012/251‑1 (MQ=255)                                                                                                                                                                                    ggATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAAACCTGATCGTCGGGGATGGAGATAACCGAGCt                                                             >  2:470127/1‑251 (MQ=255)                                                                                                                                                                                            tCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGata                                                      <  1:11309/250‑1 (MQ=255)                                                                                                                                                                                               cGTTACCGGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGTGCTGCGGATAatat                                                  <  2:393776/251‑1 (MQ=255)                                                                                                                                                                                                gTTACCTGACCGGCGACCGGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATc                                                 <  1:557668/251‑1 (MQ=255)                                                                                                                                                                                                gTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATc                                                 >  1:426573/1‑251 (MQ=255)                                                                                                                                                                                                         ccGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGccc                                        >  1:495284/1‑251 (MQ=255)                                                                                                                                                                                                             cGACCAGAATCGGCCCGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAACCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTg                                    <  2:368425/251‑1 (MQ=255)                                                                                                                                                                                                               aCCAGAATAGGCCCGCCCAACGCACCCAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATACCGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCAGCAAACGCTGATCGCCGGTGATGGAGATAACCGAGCTGCGGACAATATCGAACAGCCCGCTgcc                                  >  2:273446/1‑249 (MQ=255)                                                                                                                                                                                                                ccAGAACCGGCTCGCCCAACGCACCAACCGCCCCCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAATCCAGCCTCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCGGCACACTCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCACGCTGGCa                                 <  1:547981/251‑1 (MQ=255)                                                                                                                                                                                                                  aGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGAAATCGCCACCGGCGCACCACAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGTGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAAc                               >  1:296715/1‑251 (MQ=255)                                                                                                                                                                                                                       cGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTa                          <  2:529346/251‑1 (MQ=255)                                                                                                                                                                                                                              cccAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTtata                   <  1:581460/251‑1 (MQ=255)                                                                                                                                                                                                                              cccAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTtat                    >  1:440554/1‑250 (MQ=255)                                                                                                                                                                                                                               ccAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATAc                  >  2:239500/1‑251 (MQ=255)                                                                                                                                                                                                                                     cACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAac            >  1:197633/1‑251 (MQ=255)                                                                                                                                                                                                                                     cACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAac            >  2:199445/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaaCGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACAccg        <  1:474122/251‑1 (MQ=255)                                                                                                                                                                                                                                            cGCCACCGGCGCAATATGGGCAACCAGACACAGCCCCGCGGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACGGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAACCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATCCAGGAACAccgccg     <  2:61826/251‑1 (MQ=255)                                                                                                                                                                                                                                               cACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCACACGCTGATCGTCGGTGATGGAGCTAACCGAGCTGCGGATAATCTCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCcgcgc  >  2:552747/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 CGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGA‑ACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGA  >  NC_000913/3120539‑3121026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑