Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	290,175	G→A	T44I (ACC→ATC)	argF ←	ornithine carbamoyltransferase ArgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	290,175	0	G	A	100.0%	169.3 / NA	51	T44I (ACC→ATC)	argF	ornithine carbamoyltransferase ArgF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/36);  total (15/36)

ACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTCTTTCTTACAAAGGTGGAGGCA  >  NC_000913/289932‑290413                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               acCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCtt                                                                                                                                                                                                                                         >  2:506192/1‑251 (MQ=255)            cGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACTAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTGACCGATAAGCTTCTGGACTTCCt                                                                                                                                                                                                                              <  2:241887/251‑1 (MQ=255)             gCATACTACGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGACCCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTCAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCtt                                                                                                                                                                                                                             >  2:54277/1‑251 (MQ=255)               agaCTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGc                                                                                                                                                                                                                           <  1:586359/249‑1 (MQ=255)                 aCTGTGCCAGCGTTTCGCCCACTGCCTGGCCGTGACCGCGATACTTAATGCCGTCATACATCCGCCCGAGAACCCGCGCTGTGCCCTTAATTGACTCGTTATGCCCAATCTGGCTGCCGCCCGGCCCTAAATAGGTAACGCGCGCTCCCTGTTCAAATGCTGCAACTGCGAAAGAACAACGGGTACGAGTCGAGTCTTTTGCGAAGAGGAGCGCGATGTTTTTTCCGATTAGCTTCTGTACTTTCTTGcc                                                                                                                                                                                                                          <  2:561631/250‑1 (MQ=255)                 aCTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCTATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGcc                                                                                                                                                                                                                          <  2:507791/250‑1 (MQ=255)                 aCTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGcc                                                                                                                                                                                                                          <  1:386273/250‑1 (MQ=255)                            gTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATc                                                                                                                                                                                                              <  1:420602/251‑1 (MQ=255)                               tCGACCACTCCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACCCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCgg                                                                                                                                                                                                            <  2:263243/250‑1 (MQ=255)                                  aCCACTTCCTGGCCGGGACCGCGATACTGAATTCCGTCAAACATCCGCCCGAGAACCCTCGCGGTGTCCTTAATTGACTCTTTATGCCCTATCGGGCTGCCGCTCGGTCCTAAATAGGTAACGCGCGCGCCCTGGTCAAGTGCGGCAACTCCGAAAGAGCAACGGGTTCGTGTCGAGGCGTTTTCGAAGATGAGCGCGATGTTTTTGCCGATAATCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCttt                                                                                                                                                                                                        <  1:580878/251‑1 (MQ=255)                                  aCCACTTCCGTGCCGTGACCGCGTTACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCCGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCCCGGCCCTAATGAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTGCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCTAAGATGAGCGCGATGTTTTTACCGAGAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCttt                                                                                                                                                                                                        <  2:352354/251‑1 (MQ=255)                                         ccTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCtgt                                                                                                                                                                                                 >  2:164617/1‑251 (MQ=255)                                             gCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCgg                                                                                                                                                                                             >  2:15730/1‑251 (MQ=255)                                                  gACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAg                                                                                                                                                                                         <  1:506192/250‑1 (MQ=255)                                                     cgcgATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTTGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATTTTTTTACCGATAAGCTTCTGGTCTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTc                                                                                                                                                                                     <  2:388740/251‑1 (MQ=255)                                                              gAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAgaga                                                                                                                                                                             <  1:54277/250‑1 (MQ=255)                                                                  gCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGGCAGCAGAGAAGTGa                                                                                                                                                                        <  2:83342/251‑1 (MQ=255)                                                                                  ccGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGc                                                                                                                                                                                            <  2:123486/215‑1 (MQ=255)                                                                                  ccGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGc                                                                                                                                                                                            >  1:123486/1‑215 (MQ=255)                                                                                     agaACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTc                                                                                                                                                     <  1:382840/251‑1 (MQ=255)                                                                                     agaACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCGTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGTGGTAAAGTc                                                                                                                                                     <  2:391097/251‑1 (MQ=255)                                                                                       aaCCCGCGAGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCgg                                                                                                                                                   >  2:297537/1‑250 (MQ=255)                                                                                        aCCCGCGCGGTGGCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAg                                                                                                                                                  <  1:563551/251‑1 (MQ=255)                                                                                               cgGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTc                                                                                                                                           <  2:409052/251‑1 (MQ=255)                                                                                                ggTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTc                                                                                                                                           >  1:156878/1‑250 (MQ=255)                                                                                                   gtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGa                                                                                                                                        >  1:13057/1‑250 (MQ=255)                                                                                                        tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAgtg                                                                                                                                   <  1:506858/250‑1 (MQ=255)                                                                                                         aaTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGtt                                                                                                                                 >  1:463688/1‑251 (MQ=255)                                                                                                             gACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGtttttt                                                                                                                             <  1:275385/251‑1 (MQ=255)                                                                                                              aCTCTTTATGCCCAATCTGGCTGCCGCGCAGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAATTTCGAAAGAGCAACGGGTACGAGTCGAGGCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTGTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTGCAGAAAGTGttttctg                                                                                                                            <  2:189951/251‑4 (MQ=38)                                                                                                                                 gCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTAt                                                                                                         >  1:65709/1‑251 (MQ=255)                                                                                                                                       gCTCGGACATAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAACGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATCAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGGGCAGGGGTAAAGTCGTGCAGTTTCAGAAAGTGTTTTTTGTATACATCGGACCTTTTATCCTCGc                                                                                                   >  2:71298/1‑251 (MQ=38)                                                                                                                                          cGGCACTAAATAGGTGACGCGCGCGCCCTGTTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAATATGAGCGCGATGTTTTTACCGAAAAATTTCTGTTCTTCCTTGCCATGTTTTTTATCGGCGTTGAGCGGGGCGGCAAGGGTCAGCAGGGAAGTTTATTGTTCAGGGGTGAAATCGAGCAGGTGCAGAAAGTGGTGTTTTTATAAATCGGACATTGTATCATCGCATg                                                                                                <  2:364883/251‑1 (MQ=255)                                                                                                                                                 aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTGATCCTCGCATGGCGAAc                                                                                          <  1:164617/250‑1 (MQ=255)                                                                                                                                                              gcgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAAt                                                                            <  2:142404/251‑1 (MQ=255)                                                                                                                                                                   cccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTaaaa                                                                       <  2:206232/251‑1 (MQ=255)                                                                                                                                                                       ggTCAAATGCGGCAACTTCGAAAGAGCAACGGGTTCGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGTGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGGCATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCa                                                                   <  1:546385/251‑1 (MQ=255)                                                                                                                                                                           aaaTGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCATAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATGAAAATTCACttt                                                               <  2:351089/251‑1 (MQ=255)                                                                                                                                                                             aTGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTtat                                                             >  2:438608/1‑251 (MQ=255)                                                                                                                                                                                 ggCAACTGCGAAAGAGCACCGGGTACGAGTCGAGTCTTTGTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTGCTGTACTTCCGTGCCAGTTTTTGTATCGGCTTTGATCTGTGCGGGAAGGGTCAGCAGAGAAGTGGACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAATTGTTTTTTGTATAAATCGGACATTGTAGCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATAtgt                                                         <  1:585327/251‑1 (MQ=255)                                                                                                                                                                                         cGAACGAGCAACGGGTACGAGTCGAGTCGTTTTCGAAGATGAGCGCGAAGTTTTTACCGAGAAGCTTCTGTTCTTCCTTGCCATGTTGTGTAGCGGCTTTGAGCAGTGCGGCAAGTGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTCGAGAAAGTGGTTTCTGTATAAATCTGACATTGTATCCTCGCATGGCGAACGCCACTTATTAAAATAAAAGTCACTATATACGTGTAattat                                                 <  2:250364/251‑1 (MQ=255)                                                                                                                                                                                          gAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCATGGGTAAAGTCTAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTAATATGTGTAattatt                                                <  2:65285/251‑1 (MQ=255)                                                                                                                                                                                          gAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAattatt                                                <  2:159044/251‑1 (MQ=255)                                                                                                                                                                                           aaaGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTGTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTc                                               <  1:299917/251‑1 (MQ=255)                                                                                                                                                                                                 cAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGc                                         <  1:124576/251‑1 (MQ=255)                                                                                                                                                                                                                             aGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTca                             >  2:418063/1‑235 (MQ=255)                                                                                                                                                                                                                             aGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAATTAGTCATTTGCAACCCCATTTca                             <  1:418063/235‑1 (MQ=255)                                                                                                                                                                                                                                   gcgcGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTGTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTCTTTCTTACAAAGGTgg       <  1:242774/251‑1 (MQ=255)                                                                                                                                                                                                                                         tGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTCTTTCTTACAAAGGTGGAGGCa  >  1:341415/1‑250 (MQ=255)                                                                                                                                                                                                                                                aCCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTaa                                                     >  1:112372/1‑192 (MQ=255)                                                                                                                                                                                                                                                aCCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTaa                                                     <  2:112372/192‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               ACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACGCCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTCTTTCTTACAAAGGTGGAGGCA  >  NC_000913/289932‑290413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑