Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,887	G→A	S152N (AGC→AAC)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,887	0	G	A	100.0%	222.2 / NA	67	S152N (AGC→AAC)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/28);  total (39/28)

ATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATT  >  NC_000913/3104645‑3105130                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    atatCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTcg                                                                                                                                                                                                                                             >  1:248391/1‑251 (MQ=255)      aGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGAt                                                                                                                                                                                                                                        >  1:530913/1‑251 (MQ=255)           cGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGACGGTCGCGCAAACAACTTCGAGGATTATct                                                                                                                                                                                                                                   >  2:448587/1‑251 (MQ=255)             cAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATctgc                                                                                                                                                                                                                                 >  2:3393/1‑251 (MQ=255)                              aTGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGa                                                                                                                                                                                                                  >  2:218852/1‑249 (MQ=255)                                       cGATTACTATCGAAACCATAGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCgcagc                                                                                                                                                                                                       >  1:417683/1‑251 (MQ=255)                                       cGATTACTATCGAAAACATCGCCGGGACAGAACCAGCCGCGCGTTTGCGCTGGGAAATTATCAAAACGTTATTGATGGGGGACGATCCGAGTTCGGTCGATCTCTATTCCGTCGTTGTTGATATTTCGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCATACACCTACGCAGATTATCAGCTGAAGAACCACCTGAGGAGCCgc                                                                                                                                                                                                          >  2:357840/1‑248 (MQ=255)                                         aTTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCaa                                                                                                                                                                                                     <  2:155672/251‑1 (MQ=255)                                          ttACTATCGAAACCATCGCCGGGACAGACCCTGCCGCGCATTTGCGCCGGGCAATTATCACAACGTTATTGATGGGTGACGATCCGCGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCCAACAACTTCGCGGATTATCTGCTGAAAAACCGCCTGAAGAGCCGCAGCAAc                                                                                                                                                                                                    >  2:52651/1‑251 (MQ=255)                                           tACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACg                                                                                                                                                                                                   >  1:400898/1‑251 (MQ=255)                                            aCTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACgg                                                                                                                                                                                                  <  1:3393/251‑1 (MQ=255)                                               aTCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCt                                                                                                                                                                                               >  1:225732/1‑251 (MQ=255)                                                 cGAAACCATCGCCGGGACCGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGc                                                                                                                                                                                             >  1:160837/1‑251 (MQ=255)                                                       cATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTatc                                                                                                                                                                                        >  1:239017/1‑250 (MQ=255)                                                            ccGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGc                                                                                                                                                                                             <  2:155416/240‑1 (MQ=255)                                                            ccGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGc                                                                                                                                                                                             >  1:155416/1‑240 (MQ=255)                                                                   aGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAAAAGTATGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCa                                                                                                                                                                           <  1:543616/251‑1 (MQ=255)                                                                          gCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTa                                                                                                                                                                     <  1:322304/250‑1 (MQ=255)                                                                              cgcATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATg                                                                                                                                                                <  1:243238/251‑1 (MQ=255)                                                                                cATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATACGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGt                                                                                                                                                              >  1:88679/1‑251 (MQ=255)                                                                                 aTTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTCCAGCGTCACCATTAACATGGTg                                                                                                                                                             >  2:301795/1‑251 (MQ=255)                                                                                 aTTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGATGCGTATCATCTACAGCGTCACCATTAACATgg                                                                                                                                                               >  2:408269/1‑249 (MQ=255)                                                                                   ttGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGc                                                                                                                                                            <  2:428523/250‑1 (MQ=255)                                                                                      cgcCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGa                                                                                                                                                         >  1:101288/1‑250 (MQ=255)                                                                                        ccGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGaaac                                                                                                                                                      >  1:527511/1‑249 (MQ=255)                                                                                           ggCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAaccacc                                                                                                                                                   >  1:555917/1‑251 (MQ=255)                                                                                              aaTTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTGACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACATCGTCTCCATTAACATGGTGCCGATCCACCTTg                                                                                                                                                <  2:338851/251‑1 (MQ=255)                                                                                                  aTCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGACAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATa                                                                                                                                             >  1:307267/1‑250 (MQ=255)                                                                                                     aaaaCGTTATTGCTGGGTGACGCTCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGCAAGAAACTTTCCTTTACGGTCCGGTGGCGGACAACACCGGGCAGCCGGTTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTCCCGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACg                                                                                                                                         >  2:560462/1‑251 (MQ=255)                                                                                                      aaaCGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCg                                                                                                                                                                                                                       <  2:284329/172‑1 (MQ=255)                                                                                                      aaaCGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCg                                                                                                                                                                                                                       >  1:284329/1‑172 (MQ=255)                                                                                                      aaaCGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGt                                                                                                                                        <  2:400898/251‑1 (MQ=255)                                                                                                      aaaCGTTATTGATGGGTGACGATCCGAGGTCGGGCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTGTACGGTCAGGTGGTGGACAACGCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACGTCGCGGATGATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCGGCAGCGTCACCAGTAACATGGTGCCGAACCCGCTTGATAAACGt                                                                                                                                        <  2:301808/251‑1 (MQ=255)                                                                                                          gTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTgcgc                                                                                                                                    >  2:23407/1‑251 (MQ=255)                                                                                                               tGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTGCCTTTACGGGCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGTGAATGTCGCGCAAACAACTGCGCTGATTATCGGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGGCACTATTAACAGGGTGCTGAACCACCTGGGGAAACGTGCTCACaa                                                                                                                                <  2:532455/250‑1 (MQ=255)                                                                                                                 aTGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAat                                                                                                                              >  1:175198/1‑250 (MQ=255)                                                                                                                         cGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGCTTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCCACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGc                                                                                                                      >  1:74914/1‑250 (MQ=255)                                                                                                                             ccGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGt                                                                                                                 >  1:3488/1‑251 (MQ=255)                                                                                                                               gAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAACGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTcc                                                                                                               >  1:36162/1‑251 (MQ=255)                                                                                                                                 gTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGt                                                                                                                                                                                           >  2:75113/1‑173 (MQ=255)                                                                                                                                 gTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGAGTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGt                                                                                                                                                                                           <  1:75113/173‑1 (MQ=255)                                                                                                                                    cGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCa                                                                                                           <  1:15991/250‑1 (MQ=255)                                                                                                                                    cGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAg                                                                                                          <  1:189937/251‑1 (MQ=255)                                                                                                                                      gTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATGAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGc                                                                                                        <  2:242257/251‑1 (MQ=255)                                                                                                                                        cGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATTGTCCGCCAGGCg                                                                                                       <  1:140688/250‑1 (MQ=255)                                                                                                                                        cGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCg                                                                                                       <  2:160837/250‑1 (MQ=255)                                                                                                                                                  tCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAat                                                                                             <  1:221920/250‑1 (MQ=255)                                                                                                                                                    cGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGACAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATg                                                                                          >  1:538005/1‑251 (MQ=255)                                                                                                                                                      aCGTTGATGATATTACGATTTCGAAAGAACCTTTCATTTCCGGTCAGGTGGTGGACAACACCGGGCAGACGCTTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAACTATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGc                                                                                        >  2:290251/1‑251 (MQ=255)                                                                                                                                                        ggtgatgaTATTACGATTTCGAAAGAACCTGTCCTTGACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCTCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCGTGATAAACGTGCGCGCAAATAGCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGt                                                                                      <  2:555917/249‑1 (MQ=255)                                                                                                                                                                         ttcgaccgAACCTTTCCTTTACGGTCAGGAGGTGGACAAAACCGGGCAGCCGATTCGCTGGGAAGGACGCGAAAACAACTTCGAGGATTATCTGCTGCAAAAACGTCTGAAGAGCCGCAGCACCGGGCTGCGTATCATCTACAGCGTCACCCTTAACATGGTGCCGCACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTc                                                                     >  2:533971/8‑251 (MQ=255)                                                                                                                                                                            gAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTgg                                                                  >  2:23661/1‑251 (MQ=255)                                                                                                                                                                              aggaaccgttcctgttcgtgcAGGTGGTGGACACCACCGGGCAGCCGAGTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCGGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATGTCGCGGCATGTTCCGCCAGGCGTCACGGAAATACGGCGGTGATGAGTCGCTGAGTCTGGCa                                                                <  2:248391/231‑1 (MQ=255)                                                                                                                                                                              aaGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGc                                                                 >  1:396244/1‑250 (MQ=255)                                                                                                                                                                                   cctttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGCACCACCTTGATAAACGTGCGCACAAATATCTAGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTAGCTGATTCTGGCAATTAt                                                           >  1:432316/1‑251 (MQ=255)                                                                                                                                                                                         ctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGAc                                                     >  1:569906/1‑251 (MQ=255)                                                                                                                                                                                          tttACGGTCAGGTGGTGGACAACCCCGGGCAGCCGATTCGCTGGGAAAGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAACAGCCGCCGCAACGGGCTGCGCATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGAGCGCAACAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGCTGAGTCGCTGATTCTGGAAATTATGCAGAcc                                                    >  1:246454/1‑251 (MQ=255)                                                                                                                                                                                              cGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAAGATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAAt                                                <  2:293440/251‑1 (MQ=255)                                                                                                                                                                                                        gtgtacaccacCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTTTGGCAATTATGCAGACCGAATCTACCTTTaa                                      <  2:73002/243‑1 (MQ=255)                                                                                                                                                                                                             caacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGt                                 >  1:502541/1‑251 (MQ=255)                                                                                                                                                                                                                 acCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATGCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGc                             <  2:530913/251‑1 (MQ=255)                                                                                                                                                                                                                            gATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGACTCTTCCTTTAACCCGTATGCGGTCAGCCGtt                  >  2:139322/1‑251 (MQ=255)                                                                                                                                                                                                                              ttCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCCGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTcc                >  1:291254/1‑251 (MQ=255)                                                                                                                                                                                                                               tCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTcc                <  1:301795/250‑1 (MQ=255)                                                                                                                                                                                                                               gcGCTGGTAAGGTCGCGCAAACATCTTCGCGGATTATCTGCTGAAAATCCGTCTGAAGAGCCGTAGCAACGGGCTGCGTATCATCTACAGCGTCTCCATTAACATGGTGCCGAACCACCTTGATAAACGGGCGCACAAATATCTCGGCATGGTTCGCCAGTCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTcc                <  1:285556/249‑1 (MQ=255)                                                                                                                                                                                                                                           gcgcgCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCATCAACGGGCTGCGTATCATCTACAGCGGCCCCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGTTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAAGCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGAt   <  1:278785/250‑1 (MQ=255)                                                                                                                                                                                                                                            cgcgCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCATCCGTTCCGATGCGCGGGGAtt  <  2:101288/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATT  >  NC_000913/3104645‑3105130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑