BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,061,916	C→T	A356V (GCC→GTC)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,061,916	0	C	T	99.0%	347.1 / NA	106	A356V (GCC→GTC)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (46/57); total (48/58)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATC  >  NC_000913/3061671‑3062154

gtttttcttcGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGGCCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTg                                                                                                                                                                                                                                           <  2:327958/247‑1 (MQ=255)

ggccttcttcGGCATCGGCATGGATCTGTTTATGAACGTGGCCAGGTTGCGGGCGGCACGTTAGTGAGGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAAGCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGTATCCGTATAACAAC‑GGTATCCGCACCGCCATTGAAGCACTGGCTGCTACGCTGGACGGGACTCAGTCTCGGCATAGCAAGGTCTTTg                                                                                                                                                                                                                                           <  2:333053/248‑1 (MQ=255)

   cttcttcGGCATCGGCATGGATCTGTTTATGAATGTCTCCATGTTGCGTGCGGCCCGTTATTTATGGAGCGAAGCG‑TCACGTGAATTTGGAACACAGGACCCGAAATCACTGTCGGTGCGTACCCCCTGCCAGCCCTCCGCCAGGAGGCTGACCGATCAGGAACCCT‑TTACAACCGTTATCCGCACCACCATTGCAGGGCTGGCGCCGACCCGGTCCGGTACTCATTCACCGCATACCAACGTCTTTGACg                                                                                                                                                                                                                                        >  2:517804/1‑251 (MQ=255)

     tcttcGGCATCGTCATGGATCTGTTTATGAAATTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGAACCCACTGCCAGACCTCAGGCTGGCGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCCCCACCATTAAAGCGCTGGCTGCGACGCTGGGCGGTACTACGACACTGCATACCAACGTCTTTGACgg                                                                                                                                                                                                                                       >  2:73355/1‑249 (MQ=255)

     tcttcGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGCAAAGGATCCGTATAACAAC‑GTTATCCACACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCGTACCAACGACTTTGaaga                                                                                                                                                                                                                                       >  2:179994/1‑247 (MQ=255)

        gcggcagcggcatGGATCTGTTTATGAAGGTCGCCGTGTGGCGTGCGGCACGTAATTGATGGAGCGAGGCGGTCA‑GTGGATTTGGCGCACAGGCCCCGAAATCGCTGGCGCTGCGTCCCCACTGCCAGACCTCAGGCGGGCGCCTGACGGAACAGGAGCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCATCGTCTTTGACGAAgc                                                                                                                                                                                                                                    <  2:125495/249‑1 (MQ=255)

          ggcatcggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCt                                                                                                                                                                                                                                 >  2:397346/1‑251 (MQ=255)

           gcatcggcatGGATCTGTTTATGAACGTCGCCCTGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGTTTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCtt                                                                                                                                                                                                                                >  2:277181/1‑251 (MQ=255)

            catcggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTg                                                                                                                                                                                                                               <  1:99873/251‑1 (MQ=255)

                ggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATGTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACGCGAAATCACTGGTGCGGCGTGCCCACTGCCAGACCTCAGGCGGGAGCCTGACTGAACAGGATCCGTATAACAGC‑GTTATCCGCACTACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGttt                                                                                                                                                                                                                           <  1:236740/251‑1 (MQ=255)

                    tggctCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCt                                                                                                                                                                                                                       >  1:516354/5‑251 (MQ=255)

                     ggATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCt                                                                                                                                                                                                                       >  1:347795/1‑250 (MQ=255)

                        tCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCg                                                                                                                                                                                                                   >  2:437423/1‑251 (MQ=255)

                             ttATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACATCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtc                                                                                                                                                                                                              >  1:419699/1‑251 (MQ=255)

                                 gAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                                                                                                                                                                                                            >  1:81647/1‑249 (MQ=255)

                                 gAACGTCGCCATGTTGCGGGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCACCGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                                                                                                                                                                                                            >  1:375593/1‑249 (MQ=255)

                                    cGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCAc                                                                                                                                                                                                       >  2:152664/1‑251 (MQ=255)

                                     gTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACg                                                                                                                                                                                                      >  1:212263/1‑251 (MQ=255)

                                       cGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAAGCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCa                                                                                                                                                                                                    <  1:9939/251‑1 (MQ=255)

                                         ccATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAAAAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCAtt                                                                                                                                                                                                  >  1:301386/1‑251 (MQ=255)

                                           aTGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGc                                                                                                                                                                                                >  2:120506/1‑251 (MQ=255)

                                             gTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGcc                                                                                                                                                                                               <  2:170201/250‑1 (MQ=255)

                                              ttGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATCACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGAtt                                                                                                                                                                                                                >  2:253357/1‑232 (MQ=255)

                                              ttGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGAtt                                                                                                                                                                                                                <  1:253357/232‑1 (MQ=255)

                                                 cgtgcgGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCaa                                                                                                                                                                                          <  1:502109/251‑1 (MQ=255)

                                                         aCGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGCCGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACAccc                                                                                                                                                                                     >  1:377225/1‑248 (MQ=255)

                                                              atttatGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGatcat                                                                                                                                                                              <  1:558664/250‑1 (MQ=255)

                                                                 tatGGAGCGAAGCGGACA‑GTGGAGTTGGCGCTCAGGACCCGAAATCAATAGAGCAACGCCCCAACTGCCAGACCTAAGCCTGGAGCCTGACTGAACAAGATCCGTATAACCAC‑GTTATCCGCACCACCATTGAAGCGCTGGCGGCGACGATGGGCGGCAGTACGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGACTTCCCAGCACGAATTGCACCCAACACCCTGatcatcatc                                                                                                                                                                          >  2:387127/1‑251 (MQ=255)

                                                                  atGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATcc                                                                                                                                                                         >  1:148529/1‑251 (MQ=255)

                                                                    ggAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATcc                                                                                                                                                                         >  2:383298/1‑249 (MQ=255)

                                                                     gAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAgg                                                                                                                                                                      >  1:561040/1‑251 (MQ=255)

                                                                                  a‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAAct                                                                                                                                                         <  1:320338/251‑1 (MQ=255)

                                                                                               gcACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGt                                                                                                                                             <  1:256713/251‑1 (MQ=255)

                                                                                                 acaGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCg                                                                                                                                           >  1:481658/1‑251 (MQ=255)

                                                                                                      aCCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTGCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCa                                                                                                                                      <  1:348804/251‑1 (MQ=255)

                                                                                                       cccGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGCAGAATCAGAACTCTGCCGCACCGTCGATCCAc                                                                                                                                     >  2:215695/1‑251 (MQ=255)

                                                                                                       cccGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTATGCCGCACCGTCGATCCAc                                                                                                                                     >  2:284084/1‑251 (MQ=255)

                                                                                                       cccGAAAGCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCAc                                                                                                                                     <  2:294499/251‑1 (MQ=255)

                                                                                                          gAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTgg                                                                                                                                  >  1:144796/1‑251 (MQ=255)

                                                                                                           aaaTCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGTCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGTTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCTCACCGTCGATCCTCTGGc                                                                                                                                 <  2:561040/251‑1 (MQ=255)

                                                                                                            aaTCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGcc                                                                                                                                <  1:144934/251‑1 (MQ=255)

                                                                                                             aTCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCg                                                                                                                               <  2:423935/251‑1 (MQ=255)

                                                                                                                   ggCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATcc                                                                                                                          <  1:196978/250‑1 (MQ=255)

                                                                                                                    gcgcTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTa                                                                                                                        <  1:111326/251‑1 (MQ=255)

                                                                                                                     cgcTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTAt                                                                                                                       <  2:106937/251‑1 (MQ=255)

                                                                                                                     cgcTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCAGTACAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGCCGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCCGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTAt                                                                                                                       >  2:382741/1‑251 (MQ=255)

                                                                                                                      gcTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTAtt                                                                                                                      <  2:144796/251‑1 (MQ=255)

                                                                                                                             aCCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTg                                                                                                                <  1:478470/250‑1 (MQ=255)

                                                                                                                              cccACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAg                                                                                                              <  1:333705/251‑1 (MQ=255)

                                                                                                                                    gCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTg                                                                                                        <  1:412744/251‑1 (MQ=255)

                                                                                                                                        gACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGGCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCg                                                                                                    <  1:170325/251‑1 (MQ=255)

                                                                                                                                          ccTCAGGCTGGAGCCTGACTGAACATGATCCGTATAACAAC‑GTTATCCGCACCCCCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGATTCGCTGACCGAt                                                                                                  <  1:307555/251‑1 (MQ=255)

                                                                                                                                             cAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCATATCATCATCCAGGAAGAATCAGATCTCTGCCTCACCGTCGATCCACGGGCCGGATCCTATTACATTGAGTCGCTGACCGATc                                                                                                 <  2:535310/249‑1 (MQ=255)

                                                                                                                                                gCTGGCGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGa                                                                                                                                          >  2:534020/1‑205 (MQ=255)

                                                                                                                                                gCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCGCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGa                                                                                                                                          <  1:534020/205‑1 (MQ=255)

                                                                                                                                                  tGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGt                                                                                          >  1:87648/1‑251 (MQ=255)

                                                                                                                                                     aGCCTGACTGCCCAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTCCTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTcc                                                                                        >  2:221777/1‑249 (MQ=255)

                                                                                                                                                     aGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCaa                                                                                       <  1:482380/251‑1 (MQ=255)

                                                                                                                                                          gacggaACAGGATCCGTATAACAAC‑GTTAGCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGAGCAAATCGTCAAACAAg                                                                                  <  1:203744/247‑1 (MQ=255)

                                                                                                                                                           actgaACAGGATCCGTATAACAAC‑GTTAGCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCACCACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGc                                                                                 <  1:429077/251‑1 (MQ=255)

                                                                                                                                                               aaCAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATCCCACCGTATTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATAAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTCTTACATTCAGTAGATGACCGATCAAATCGTCAAACAAGcc                                                                                >  2:307216/1‑248 (MQ=255)

                                                                                                                                                               aaCAGGACCCGTATAACGAG‑GATATCAGCACCACAATTGAAGCGCTAGCTGCGACGCTGAGAGGTACTCAGTCACTGCATACCAAAGTCTTTGAAGAGACGCCTGGCATGCCTCCCGCTTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAGTCAGCACTCTGCCGCACCGTCGCTCCACTGGCCGGATCCTATTTCATTGAGACGCTGACCGAt                                                                                                  >  2:527154/1‑230 (MQ=255)

                                                                                                                                                                aCAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCagag                                                                            <  1:235578/251‑1 (MQ=255)

                                                                                                                                                                aCAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCag                                                                              >  1:536154/1‑249 (MQ=255)

                                                                                                                                                                   ggATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCta                                                                         <  1:446176/251‑1 (MQ=255)

                                                                                                                                                                         gTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCTATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATcc                                                                   <  1:142499/251‑1 (MQ=255)

                                                                                                                                                                         gTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATcc                                                                   <  1:209938/251‑1 (MQ=255)

                                                                                                                                                                          tataACAAC‑GTTAACCGCACAACCATTGAAGCGCTGGCTGCGACGCTAGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGCAGACTCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGCAAAACAAGCCAGAGCTATTCTcc                                                                   >  2:424499/1‑250 (MQ=255)

                                                                                                                                                                           agaacaac‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCGGGGCGGTACCCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCTGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCCGAGCGATTATCCa                                                                  <  2:375593/248‑1 (MQ=255)

                                                                                                                                                                             aacaac‑GTTAGCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACa                                                               <  2:81647/251‑1 (MQ=255)

                                                                                                                                                                               caac‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGCGCTATTATCCAAc                                                                >  2:360530/1‑248 (MQ=255)

                                                                                                                                                                                aac‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAAc                                                                >  2:426610/1‑247 (MQ=255)

                                                                                                                                                                                 ac‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTCCTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACCACCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATcccac                                                                >  1:491560/1‑243 (MQ=255)

                                                                                                                                                                                     ttATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATcga                                                         <  2:474055/250‑1 (MQ=255)

                                                                                                                                                                                         ccGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGaa                                                     <  1:334061/250‑1 (MQ=255)

                                                                                                                                                                                         ccGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCGATTAGCCAACAGATCGACGaa                                                     <  1:472167/250‑1 (MQ=255)

                                                                                                                                                                                          cGCACAACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCCTACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGCTTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAACCAAGCCAGAGCTATTATCCAACAGATCGACGAAGc                                                   >  2:584383/1‑251 (MQ=255)

                                                                                                                                                                                           gCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGCTCAAATCGTCCAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGcc                                                  >  2:39622/1‑251 (MQ=255)

                                                                                                                                                                                            caccacCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCg                                                 >  1:395621/1‑251 (MQ=255)

                                                                                                                                                                                            caccacCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATGTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTAGCCAACAGATCGACGAAGCCg                                                 <  2:348639/251‑1 (MQ=255)

                                                                                                                                                                                                 ccaTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGc                                            <  1:478560/251‑1 (MQ=255)

                                                                                                                                                                                                      gAAGCGCTGGCTGCGTCGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATGTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATgg                                        <  1:354272/250‑1 (MQ=255)

                                                                                                                                                                                                       aaGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGACGCCGGTGGCATGGCg                                      >  1:25285/1‑251 (MQ=255)

                                                                                                                                                                                                       aaGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCg                                      >  1:587868/1‑251 (MQ=255)

                                                                                                                                                                                                        aGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGa                                     <  1:39622/251‑1 (MQ=255)

                                                                                                                                                                                                          cgctgagtgccaCGCTGGGCGCCACTCAGTCACTGCATGCCAACGTCTTTGACGAAGCGCTTGGTATGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCACATCGTCAACCAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGAGGCCTGGCGaa                                    >  2:535655/12‑250 (MQ=255)

                                                                                                                                                                                                           gctggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGaa                                    >  1:481463/1‑249 (MQ=255)

                                                                                                                                                                                                              ggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGc                                 <  1:215695/249‑1 (MQ=255)

                                                                                                                                                                                                                 tgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCg                            >  2:356467/1‑251 (MQ=255)

                                                                                                                                                                                                                    gACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCa                                                                                                <  1:396007/180‑1 (MQ=255)

                                                                                                                                                                                                                     aCGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCCACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGc                        >  1:35417/1‑251 (MQ=255)

                                                                                                                                                                                                                            gCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGAGTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTg                 <  1:500146/251‑1 (MQ=255)

                                                                                                                                                                                                                              ggTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGcc               <  1:437423/251‑1 (MQ=255)

                                                                                                                                                                                                                                tACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAACTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCa              >  1:315355/1‑250 (MQ=255)

                                                                                                                                                                                                                                 aCTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCaaa            <  1:397346/251‑1 (MQ=255)

                                                                                                                                                                                                                                 aCTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCaaa            <  1:73355/251‑1 (MQ=255)

                                                                                                                                                                                                                                   tCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACGAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAAc          >  1:283900/1‑251 (MQ=255)

                                                                                                                                                                                                                                    cAGTCACTGCATACCAACGTCTTGGACGAAGCGCTTGGTTTGCCTACCGAGTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACGGGCCGGATCCTATTTCATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCTGTGGCATGGCGAAAGCGATCGAAGCAGTTCTGCCAAAAc          <  2:377225/250‑1 (MQ=255)

                                                                                                                                                                                                                                         ccTGCATCCCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGa    <  1:362864/250‑1 (MQ=255)

                                                                                                                                                                                                                                            gCATACCAACGGCTTTGACGAAGCGCTTGGTCTGCCTACCTATTTCTCAGCACGCATTGCCCGCAACACCCAGACCATCAACCAGGAAGAATCAGAACTCTGCCGCACCGTCGAGCCACTGGCGGGATCCTTTTACATTGAGTCGCTGACCGATTAAATCGTCAAACAAGCCAGAGCTAGTATCCAACAGATCGACGACGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATc  <  1:517804/250‑1 (MQ=255)

GTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCA‑GTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAAC‑GTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATC  >  NC_000913/3061671‑3062154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`