Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,804,133	C→T	S211F (TCT→TTT)	nrdF →	ribonucleoside‑diphosphate reductase 2 subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,804,133	0	C	T	100.0%	109.7 / NA	32	S211F (TCT→TTT)	nrdF	ribonucleoside‑diphosphate reductase 2 subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

CAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAA  >  NC_000913/2803916‑2804378                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      cAGCAACCTTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCg                                                                                                                                                                                                                       <  2:48171/250‑1 (MQ=255)     aagaTTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTAGCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGTCAACGTGAAGAGTTGAAGAGTTTCGCCTTc                                                                                                                                                                                                                  <  1:473183/248‑1 (MQ=255)     aaCATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTc                                                                                                                                                                                                                  >  1:256379/1‑251 (MQ=255)        aTTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACAGGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGAt                                                                                                                                                                                                               <  2:339155/251‑1 (MQ=255)                             tGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGAc                                                                                                                                                                                          >  1:501682/1‑251 (MQ=255)                               aaaaaGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACa                                                                                                                                                                                         >  2:20072/1‑250 (MQ=255)                                                              cTTTTTTGTTCTATTCCGGTTTCTGGCTGCAGATGTATTTTTCCAGCAGCGGAAAGCTGACCAATACAGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGACTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATCCACCGATGAGCTGTACGcc                                                                                                                                                         >  2:86485/1‑251 (MQ=255)                                                                  tttGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGaa                                                                                                                                                      >  2:296893/1‑250 (MQ=255)                                                                       tCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGCACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCg                                                                                                                                                >  1:228895/1‑251 (MQ=255)                                                                             ccGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCt                                                                                                                                          <  1:104450/251‑1 (MQ=255)                                                                                          gCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAg                                                                                                                             <  2:195140/251‑1 (MQ=255)                                                                                                  aTTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTTATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGAGGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTTAAAGCGTTtctc                                                                                                                     <  2:397013/251‑1 (MQ=255)                                                                                                      ttCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGt                                                                                                                  <  2:147649/250‑1 (MQ=255)                                                                                                            ccGCGGAAAGCTGACCAATCTCGCGGACCTGATCCGTCTTATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGGAAGTCGAAACCTCGTGGGCTGACGATGTGAAAGCGGTTGTCTGTTACAACg                                                                                                           <  2:348800/251‑1 (MQ=255)                                                                                                              gaggAACGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGcc                                                                                                         >  2:261368/3‑251 (MQ=255)                                                                                                                                    ggACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTgg                                                                                   >  2:137109/1‑251 (MQ=255)                                                                                                                                      aCCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGc                                                                                 >  2:154505/1‑251 (MQ=255)                                                                                                                                          gATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTa                                                                               <  1:154505/249‑1 (MQ=255)                                                                                                                                                         ccGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGATCTGTACGCCGAAACCCCGTGGGCTGACGAGGTGAAAGCGGGTCCCTGTTACAACGCCAATAAGGCttt                                                                                              <  2:69811/219‑1 (MQ=255)                                                                                                                                                         ccGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCg                                                              <  2:434414/251‑1 (MQ=255)                                                                                                                                                                      gTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAAt                                                                                                                                                                             <  2:191940/127‑1 (MQ=255)                                                                                                                                                                      gTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAAt                                                                                                                                                                             >  1:191940/1‑127 (MQ=255)                                                                                                                                                                         cACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAgg                                              >  2:225972/1‑250 (MQ=255)                                                                                                                                                                                             taAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGCAGAGTTTCGCCTTCGATTTGTTGCTGGCACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAAACCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTcgccg                          >  1:359874/1‑251 (MQ=255)                                                                                                                                                                                               aagatCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCcgcg                        <  2:113991/248‑1 (MQ=255)                                                                                                                                                                                                 tatCAGAAACACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCATCGCCGCGCt                      >  2:16670/1‑251 (MQ=255)                                                                                                                                                                                                  atCAGAAAAACATGGAAAAGATATTTCTGGGACCACGTGAAGAGTTGACGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGCGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGACTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAACTGGCGGAAGTGAATCCGGCACTCCTCGCCGCGCtt                     >  2:581672/1‑251 (MQ=255)                                                                                                                                                                                                           aCATGGAAAAGATATTTCTGGGACAACGTGACGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACAAGTTGCAATACACCGATGAGCTGTACGCAGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAAt                                  >  1:254932/1‑229 (MQ=255)                                                                                                                                                                                                           aCATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAAt                                  <  2:254932/229‑1 (MQ=255)                                                                                                                                                                                                            cATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATg           <  1:86485/251‑1 (MQ=255)                                                                                                                                                                                                                     gATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGCTGaa   >  1:41496/1‑250 (MQ=255)                                                                                                                                                                                                                     gATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGaaa  <  1:121852/251‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      CAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAA  >  NC_000913/2803916‑2804378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑