Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,410,419	G→A	L312L (CTG→TTG)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,410,419	0	G	A	100.0%	199.5 / NA	58	L312L (CTG→TTG)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/26);  total (32/26)

TGGCCAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCG  >  NC_000913/2410173‑2410662                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    tGGCCAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATAcc                                                                                                                                                                                                                                                 >  1:124105/1‑251 (MQ=255) gggCCAGCAGACCGACCAGTTTCCAGTTACCCACAACCAGGAGGATATCGGCCAGCAGCAGAGGCTCAACCGCCCGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTGGCGGTTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGAATCAGGAATTAATGAACTCCCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATAc                                                                                                                                                                                                                                                  <  2:191169/249‑1 (MQ=255)        cATACCGATCAGTTTCAAGTTAACAACAACCAGGATGCTATCGCCACGCACCCGCGCCTCATGCGACAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTCGCCGCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGCTCTGACTCAGGAATTAATGAAATCTCTGCCCTGCCCACATCAAGGGCCTGGACAGAAAAATACTCGCCGCCCAATACCATCGGcc                                                                                                                                                                                                                                          >  2:260091/1‑250 (MQ=255)            ccGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCTTCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCCCATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAg                                                                                                                                                                                                                                      <  1:397281/251‑1 (MQ=255)             cGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGc                                                                                                                                                                                                                                     >  1:441457/1‑251 (MQ=255)              gATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGc                                                                                                                                                                                                                                     >  2:63657/1‑250 (MQ=255)                 cAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAAtt                                                                                                                                                                                                                                 >  2:526803/1‑251 (MQ=255)                 cAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAAtt                                                                                                                                                                                                                                 >  2:457051/1‑251 (MQ=255)                       ccAGTTACCCACAACCAGGCTGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGCAAACCAATTTCGCGCACCGATTTCCCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCAc                                                                                                                                                                                                                           >  1:424378/1‑251 (MQ=255)                       ccAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCAc                                                                                                                                                                                                                           >  1:513843/1‑251 (MQ=255)                       ccAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCAc                                                                                                                                                                                                                           >  2:196840/1‑251 (MQ=255)                                cacaACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACGAAAtt                                                                                                                                                                                                                  <  2:289785/251‑1 (MQ=255)                                                    ccAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGcc                                                                                                                                                                                              <  2:74950/251‑1 (MQ=255)                                                     cagcagcagAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCAGCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCg                                                                                                                                                                                             <  2:498612/251‑1 (MQ=255)                                                        cagcagAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAg                                                                                                                                                                                          <  2:283685/251‑1 (MQ=255)                                                                   cATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCaa                                                                                                                                                                                 <  1:440117/249‑1 (MQ=255)                                                                       agCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCCCATTCAGTCCGTAGCGGGTCCGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGCCTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACCACACAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGcc                                                                                                                                                                           >  2:308845/2‑250 (MQ=255)                                                                         ccAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCaa                                                                                                                                                                          >  1:20920/1‑250 (MQ=255)                                                                          cAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCaaa                                                                                                                                                                         >  1:7145/1‑250 (MQ=255)                                                                                    tCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAcgcg                                                                                                                                                               <  2:116751/250‑1 (MQ=255)                                                                                            cacgccaTTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTCATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAcgcgcgcg                                                                                                                                                           >  1:125599/1‑246 (MQ=255)                                                                                            cacgccaTTGCGCTTTAGCCCCACCACAGTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAcgcgcgcg                                                                                                                                                           <  2:125599/246‑1 (MQ=255)                                                                                             acgccaTTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCGGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGGCAGCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTc                                                                                                                                                     <  1:88383/251‑1 (MQ=255)                                                                                                   ttgtgcTTTAGCCCCATCACAATAAGTCCGTACCGGGTCCGAAAACCAATTTCGCCCCCCGATTTACCAATCAGGTCTGACTCAGCAATTAATGAAATCTCTGCCATGCCCACATCAAGCGCCTGGACTGAAAAATACTCGCCCCTCAATACCATCGGCTCCAGCAATTGAACAATACAACCTGGCCGGAGATCAGCCTCACCCGCAGACATATCAATAAACAAACCGTAACGCGCGCCAAACTCAGacac                                                                                                                                               >  2:130710/5‑248 (MQ=255)                                                                                                      cgcTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAAcccc                                                                                                                                            <  2:124105/251‑1 (MQ=255)                                                                                                            aGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAAc                                                                                                                                      <  1:63657/251‑1 (MQ=255)                                                                                                              ccccACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGt                                                                                                                                    <  1:340329/251‑1 (MQ=255)                                                                                                                  accacATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCAc                                                                                                                                >  1:135815/1‑251 (MQ=255)                                                                                                                       agtCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGGTCACGATaa                                                                                                                           <  2:8247/249‑1 (MQ=255)                                                                                                                           aGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAAcgcg                                                                                                                       <  1:574551/251‑1 (MQ=255)                                                                                                                               cGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGcgacg                                                                                                                    >  1:212149/1‑250 (MQ=255)                                                                                                                                gTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGcgacga                                                                                                                   >  1:63409/1‑250 (MQ=255)                                                                                                                                      ggTACGAAAACCAATTTCGCGCTCCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAATGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTGCACGATAACGCGACGAAAAcg                                                                                                              <  1:330260/249‑1 (MQ=255)                                                                                                                                           gAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACgccgcc                                                                                                         >  2:345981/1‑249 (MQ=255)                                                                                                                                                       cgcgCACGGATTTACCAGTCAGTTCTGGCTCAGGGTTTAGTGATATCTCTGCCAGGCCCACATCGGGGGCCTGGTCAGAAAGATACTCGCCGCGCAATACCATCGGCGCCGGCAATTGCTCACTACAAAATTGCCGGAGATCGGCATCAGCCGCAGACATATCAATAAGCAAACCGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGACAACGCCGCCAGCGTTCAACAcc                                                                                            <  2:559006/250‑1 (MQ=255)                                                                                                                                                       cgcgCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACAcc                                                                                            <  1:50979/250‑1 (MQ=255)                                                                                                                                                       cgcgCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACAcc                                                                                            <  1:257069/250‑1 (MQ=255)                                                                                                                                                                 tttACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGtt                                                                                   >  1:197814/1‑249 (MQ=255)                                                                                                                                                                   tACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAgc                                                                                <  1:85873/250‑1 (MQ=255)                                                                                                                                                                        aTCAGTTCTGACTCAGGAATTCATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCCGCGTTCAACACCGATGACGTTAGCGCCAt                                                                           >  2:527134/1‑250 (MQ=255)                                                                                                                                                                                    tCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTa                                                               >  1:462425/1‑250 (MQ=255)                                                                                                                                                                                     cAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATa                                                                                                                            >  1:425666/1‑188 (MQ=255)                                                                                                                                                                                     cAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATa                                                                                                                            <  2:425666/188‑1 (MQ=255)                                                                                                                                                                                     cAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCGCCAGCAATTGCTCACTACAAAATTGACGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTa                                                               <  2:441457/249‑1 (MQ=255)                                                                                                                                                                                      aGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAAtt                                                            <  2:197814/251‑1 (MQ=255)                                                                                                                                                                                              aTGACATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCAGAGATCGACATCAGCCGCAGACATATCACTAAGCAAAACGACACTCGCGCGAAATTCAGAAACCACATTAACGTTCACGCTAACGCGACTAAAACGCCGCCAGCGTTCAACACCACTGACGTTAGCTCCATAACGCTCCCGTAATTTGAGatc                                                     >  2:117189/1‑250 (MQ=255)                                                                                                                                                                                                       ctGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGt                                           >  1:295738/1‑251 (MQ=255)                                                                                                                                                                                                         gCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCg                                            <  1:457051/248‑1 (MQ=255)                                                                                                                                                                                                           cATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGAc                                       >  1:262259/1‑251 (MQ=255)                                                                                                                                                                                                                ccACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCTGCTGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATc                                  >  1:199032/1‑251 (MQ=255)                                                                                                                                                                                                                    aTCAAGGGCCTGGTCAAAACAATAATCGCATCGCAATACCATCGGCTCCTGCAATTGCTCACTAAAAAATTGCAGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTAAGAAACCCCATTAACGTTCACGATACCGCGTCGCAAAAGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAGGTAATTTACGTACATCCAGCCGGTGACCAATCATTg                              >  2:487464/1‑251 (MQ=255)                                                                                                                                                                                                                         gggCCGGGACAGAAAAATACTCACCGCGCAATACCATCGGCTCCAGCACTTGCTCACTACAAAATTGCCGGAGATCGCCATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCATCGTTCAACACCGTTGACGTTAGCGCCATAACGCTCACGTAATTTGAGCTCATCCAGCCGTTGACCAATCATTGGCGAt                         >  2:421738/1‑251 (MQ=255)                                                                                                                                                                                                                           gCCTGGTCAGAAAAATACTCGCCGCGCAATACCAGCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGAGCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc                       <  2:135815/251‑1 (MQ=255)                                                                                                                                                                                                                                    gAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAAt              >  1:406735/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaaaTACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGGTCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAAt              >  1:544004/1‑249 (MQ=255)                                                                                                                                                                                                                                         aTACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCCGCCGCAGACATATCAATAAGCAAAACGTAACGCGCGCGAAATTAAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCACCACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGcc          >  1:388449/1‑250 (MQ=255)                                                                                                                                                                                                                                              cgccgcGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGt    <  1:379521/251‑1 (MQ=255)                                                                                                                                                                                                                                                ccgcGCAATACCAGCGGCTCCAGCACTTGCTCACTACAAACTTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGCTCCGGGGCGAATAGCCAGACGTcg  >  2:419698/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    TGGCCAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCG  >  NC_000913/2410173‑2410662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑