Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,384,808 C→T K305K (AAG→AAA)  yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,384,8080CT97.4% 116.0 / NA 40K305K (AAG→AAA) yfbKIPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (15/23);  minor base G (0/1);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATC  >  NC_000913/2384569‑2385042
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
aCTTTCTGCGCTTCAGAGAGGGCATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCctg                                                                                                                                                                                                                                 >  2:9554/1‑251 (MQ=255)
              agagagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCCCCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGtt                                                                                                                                                                                                                   >  2:344138/1‑251 (MQ=255)
                     gTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGccc                                                                                                                                                                                                            <  1:495385/251‑1 (MQ=255)
                               aGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCAcc                                                                                                                                                                                                      <  1:451974/247‑1 (MQ=255)
                               aGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCAcc                                                                                                                                                                                                      >  2:451974/1‑247 (MQ=255)
                                                        ggCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCTCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAATTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCg                                                                                                                                                                         <  1:396295/251‑1 (MQ=255)
                                                                    catcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGACTCAATTGCgg                                                                                                                                                             >  1:37224/1‑251 (MQ=255)
                                                                          tGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCAt                                                                                                                                                                               >  1:266226/1‑227 (MQ=255)
                                                                          ggCCTCGTTGTAATTGCTATCCCCCCCGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTCCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCAt                                                                                                                                                                               <  2:266226/226‑1 (MQ=255)
                                                                               cGTTGTAATTGCTATTCCCCCCGCCAAACGTCGACAGAGTAACACCAGCCTCCAGCTGTTTTTTGACCATCGATTCAATCGATTTTGGACCGTCAATGCCAACGTTAAAGCCGCCGTCAGTGGTTAATAAAATGCGAGTGATGCCGCCTTTAA‑AACCCCTT‑TCCTCGCCTGGTGTTAGGCCATTTCCAGCCCGGCTCCGCCATTGGTCCTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCg                                                                                                                                                 <  1:93065/251‑1 (MQ=255)
                                                                                tttGTAATTGCTATTCCCACCGCCAAACGTCGACAGAGTAACCCCAGACCCCCGCTGTTTTTTGACCATTGATACAATCTATTTTGAATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGTCTAATAAAGTGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAATCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTcc                                                                                                                                                  <  2:217281/249‑1 (MQ=255)
                                                                                gTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCg                                                                                                                                                 >  2:246569/1‑251 (MQ=255)
                                                                                  tGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGc                                                                                                                                                <  1:148667/250‑1 (MQ=255)
                                                                                   gTAATTGCTATTCCCCCCGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGCCCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGcc                                                                                                                                               <  1:288822/250‑1 (MQ=255)
                                                                                    tAATGGCTATTCCCCACGCCAAACGCCGACAGAGTAACCCCAGACTCCCGCTTTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCATTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCt                                                                                                                                              <  1:37644/250‑1 (MQ=255)
                                                                                        tGCTATGCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTGTGTCCATTTATTCAATCGATTTTGTATCGTCAATCCCAACGTTAAAGTCTCCGTCAGTGGCTAATAAAATTCGAGTAATGCCGCCCTTAATAGACCTTC‑TCGACGCCTGCTGATAAGCCAGTGCCAGCCCGGCACCGCCATTGATACTGCCTGCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATg                                                                                                                                          <  1:338615/250‑1 (MQ=255)
                                                                                           tATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGa                                                                                                                                         >  2:78743/1‑248 (MQ=255)
                                                                                           tATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTAAAAGCCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑GCGTCGCCGGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGa                                                                                                                                         <  1:78743/248‑1 (MQ=255)
                                                                                                           cGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGAGCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAA‑CCCCTGTCTTCGCCGGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTTCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgca                                                                                                                      <  1:497329/251‑1 (MQ=255)
                                                                                                                aCAGAGTAACACCATACTCCCGCTTTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCACCGTTAAAGTCACCGTCAGTGGTTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaatgca                                                                                                                 <  1:449229/251‑1 (MQ=255)
                                                                                                                                        gTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAACCCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaat                                                                                                                    <  2:202321/224‑1 (MQ=255)
                                                                                                                                        gTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaat                                                                                                                    >  1:202321/1‑224 (MQ=255)
                                                                                                                                        gTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTAAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAACCCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAgg                                                                                           <  1:23020/249‑1 (MQ=255)
                                                                                                                                             ttGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGc                                                                                                                                                               >  2:341988/1‑176 (MQ=255)
                                                                                                                                             ttGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGc                                                                                                                                                               <  1:341988/176‑1 (MQ=255)
                                                                                                                                                     tGATTCAATCACTTTTGGATCGGCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGCTGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCCGCCCGGCACCGCCATCGGCACTGCCTTCGGCATCCAGCGAATCCATTGCGGCATTAATTTCCGCCTTATGACTCCAGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGt                                                                            >  2:379054/1‑251 (MQ=255)
                                                                                                                                                                    tGGATCGTCAATGCAAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGAGATTGATGCAGCCCTTAATAAACCCTT‑TCGTAGACTGATGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGa                                                              >  1:85806/1‑250 (MQ=255)
                                                                                                                                                                    tGGATAGTCAATGCAAAAGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTACTTAACCCTT‑TCGTCGCCTGCTGATAAGGCAGTTCCCGCCAGGCACCGCCATTGGTACTGACTTCGGCATCCAGCGCATCCATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGACGGCAATGCAATACGGGAGTCGCCAACGTAGGTCACGATGGCCATGTTATCCTGATCACGa                                                              >  2:520803/1‑250 (MQ=255)
                                                                                                                                                                     ggATCGTCAATGCCACCTGTAAAGTCCCCTTAAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGAAAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAg                                                            <  1:415164/251‑1 (MQ=255)
                                                                                                                                                                                        tAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAACCCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAAttt                                          <  1:88788/250‑1 (MQ=255)
                                                                                                                                                                                         aaaGTCACCGTCAGGGGCTAATAAAAGGCGATTGAGGCCGCCCTTAAAAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAATTTCTTTGACCAATAATTTc                                         <  2:506428/250‑1 (MQ=255)
                                                                                                                                                                                          aaGTCACCGTCAGTGGCTAATAAAATGCGATTGAGGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCaa                                       <  1:87977/251‑1 (MQ=255)
                                                                                                                                                                                               aCCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGa                                    >  1:211394/1‑249 (MQ=255)
                                                                                                                                                                                                ccGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAACCCCTT‑TCTCCGCCTGCTGATAAGCCAGTTCCAGCCCGTCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCGTTGACCAATAATTTCAACGAAGa                                 <  2:509851/251‑1 (MQ=255)
                                                                                                                                                                                                   tCAGTGGATAATAAAATGCGATTACTGCCGCCCTTAATACACCATT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAAAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGACGTTCTTTGACCAATAATTTCAACGCAGACTg                              >  2:51536/1‑251 (MQ=255)
                                                                                                                                                                                                      gTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGAt                           >  1:574032/1‑251 (MQ=255)
                                                                                                                                                                                                       gggCTAATAAAATGCGATTGATGCCGCCCTTAATAACCCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATa                          <  2:535204/250‑1 (MQ=255)
                                                                                                                                                                                                               aaaagtcgattgcggccgccCTAAATAACCCCGT‑CCGTCGCCTGTTGTTAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCaa                  <  2:267527/237‑1 (MQ=255)
                                                                                                                                                                                                                 aaTGCGATTGATGCCGCCCTAAATAACCCCTT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCCCCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATGTCTACGAAGACTGGATAAGTGGCAAAc                <  2:36389/251‑1 (MQ=255)
                                                                                                                                                                                                                               cgccCTTAATAAACCCTT‑GCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGGCCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATc  <  1:344138/251‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
ACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCT‑TCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATC  >  NC_000913/2384569‑2385042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑