Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,162	C→T	D87N (GAC→AAC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,162	0	C	T	96.6%	188.1 / NA	59	D87N (GAC→AAC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/33);  minor base G (1/1);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAGGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTC  >  NC_000913/2338920‑2339406                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     cagcagGTGAGGAATTTTGGGTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGAccg                                                                                                                                                                                                                                              <  2:279506/251‑1 (MQ=255)   gcagGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGAccgcc                                                                                                                                                                                                                                            <  1:152921/251‑1 (MQ=255)   gcagGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGAccgcc                                                                                                                                                                                                                                            <  1:487126/251‑1 (MQ=255)      ggTTAGGAATTTTGGTTTGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCATCTGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGCTCGTACCGACGTTACCCTGACCGTCTACCAACATATAACGCAGCGAGAATGGCTGCGCCAAGCGGACGATCGTGTTATAGAccgcag                                                                                                                                                                                                                                           >  2:3719/1‑247 (MQ=255)                  tggttggCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAGTCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGGCACCATgggg                                                                                                                                                                                                                              <  1:463299/250‑1 (MQ=255)                           tGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTAttt                                                                                                                                                                                                                     >  2:328881/1‑250 (MQ=255)                              cGTCCGGAATTTTTTCCGTGCCTTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTACGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGATTCACCATGGGGATGGTATTTACCg                                                                                                                                                                                                                 <  2:510038/251‑1 (MQ=255)                              cGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCg                                                                                                                                                                                                                 >  1:397852/1‑251 (MQ=255)                                 ccGGAATTTTTTCCGTGCCGGCAGAGTTATCAACGAAATCGACCGTCTCTGTTTCGAGACCGGCCAACAGTTCATGGGAAATTTGCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTGATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGAtt                                                                                                                                                                                                              <  2:501907/251‑1 (MQ=255)                                       ttttttCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTc                                                                                                                                                                                                         >  1:401801/1‑250 (MQ=255)                                          tttCCGTGCCGTCATAGTTATCAACGAAATCGTCCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCAcc                                                                                                                                                                                                      <  2:120140/250‑1 (MQ=255)                                          tttCCGGGGCGTCATAGTTATCAACGAAATAGACCGACTCTTTTTCGGGATCGACCATCAGTTCGTGGGCACTTTTCGCCAGAAGGATTTACGTATACCGCATTGCTGCCGCAGCGTCGCCGACGCTAGAACCGAGGTTACCCTGACCGTATACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGGTCGTGTTATCGGACGCCGAGCCACCATGGGGCAGGTGTTTAACGTTTACATCAcc                                                                                                                                                                                                      >  1:448217/1‑250 (MQ=255)                                             ccgtgccgtgATATTTATCAACAAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCTTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTTCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGTGGATGGTATTTACCGATTACGTCACCAAc                                                                                                                                                                                                   <  2:486092/250‑1 (MQ=255)                                                  ccGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGGTTACGTACCCAACGACACg                                                                                                                                                                                             >  2:53245/1‑251 (MQ=255)                                                          gTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTGTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGCGTTATACACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGAtt                                                                                                                                                                                     >  1:449704/1‑251 (MQ=255)                                                                aaCGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGACCGATTTTTtat                                                                                                                                                                               >  2:71571/1‑251 (MQ=255)                                                                             gTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTGCCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCa                                                                                                                                                                  <  1:475643/251‑1 (MQ=255)                                                                                 ctTTTTCGAGACCGGCCATCAGTTCATGGTCAATTGTCGCCATACGGATTCCCGTATTACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGGTCCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGAAGATCGTGTTATAGACCGCCGAGGCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTACAg                                                                                                                                                                 <  2:312205/248‑1 (MQ=255)                                                                                    tttCGAGATCTGCAATCAGTTCATGGGCTATTGTCGGCCGACGGATTTACGTATAACGTATTGCCGCCGCAGAGTCGCAGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGTCTGCGCCATGCTGACGATCGTGTTATAGCCCGCCGAGTCACCATGTGGTTGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTGGTTCCAGTCAtt                                                                                                                                                            <  1:347999/250‑1 (MQ=255)                                                                                       cGAGATCGGCCCTCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCGGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGGTTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCt                                                                                                                                                        >  2:51485/1‑251 (MQ=255)                                                                                           aTCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGt                                                                                                                                                     >  1:429934/1‑250 (MQ=255)                                                                                                    cAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATgg                                                                                                                                           <  1:573408/251‑1 (MQ=255)                                                                                                      gTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGCCCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCg                                                                                                                                         <  1:312213/251‑1 (MQ=255)                                                                                                         cATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCAATGCGGACGATCGTGTTATAGACCGCCGAGTCACCCTGGGGATGGTATTTACCGATTAAGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTa                                                                                                                                       >  1:267769/1‑250 (MQ=255)                                                                                                           tGGGCAATTTTCGCCAGACGTATTTCCGTATACCGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTaaa                                                                                                                                     <  2:397852/250‑1 (MQ=255)                                                                                                           tGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTaaat                                                                                                                                    >  1:76709/1‑250 (MQ=255)                                                                                                                       gCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGgtg                                                                                                                        >  2:438125/1‑251 (MQ=255)                                                                                                                          aGACGGATTTCCGTATACCGCATTGCCGCCGCAGAGGCGCCGTCGATAGAACCGAAGTTACCCTGACCGGCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGGGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTGTGTTCCAGTCAGTGACTAGTACGTTCATGGCGTAAAGTACGCGACGGGGTAc                                                                                                                     <  2:463985/251‑1 (MQ=255)                                                                                                                            aCGGATTTCCGTATAACGCATTGCCGCCGCAGAGACGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATAGAACGCAGCGAGAATGGCTGCGCCATGAGGACGATCGGGTGATAGACCGCCGAGTCACCATGGGGATGGTATTTACTGATTACTTCACCAACGACACGGGCAAGTTTTTTATAGGCTTTGTTCTAGTCATTGCCTAGTACGATCAGGGCGTAAGGTAGGCGACgggggaccg                                                                                                                   >  2:132269/1‑244 (MQ=255)                                                                                                                               gATTTCCGTATAACGCTTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCt                                                                                                                <  1:328881/251‑1 (MQ=255)                                                                                                                                    ccGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGCAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg                                                                                                           >  2:402809/1‑251 (MQ=255)                                                                                                                                                   ccgccgCAGAGTCGCCGTCGATATAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTATTATAGACCTCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTTTTCCAGTCATTGCCTAGTACGTTCAGGGCTTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCt                                                                                            <  1:526264/251‑1 (MQ=255)                                                                                                                                                    cgcctcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTg                                                                                           <  2:448453/251‑1 (MQ=255)                                                                                                                                                    cgccgcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTACAGTACGCGACGGTGTACCGACTTCAGGCCATCTCGGACATCTg                                                                                           >  2:293184/1‑251 (MQ=255)                                                                                                                                                                   gTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCca                                                                             >  1:136352/1‑250 (MQ=255)                                                                                                                                                                     cGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGAcaac                                                                           >  2:305051/1‑250 (MQ=255)                                                                                                                                                                      gATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGGGTACCGGCTTCAGGCCATATCGGACATCTGGCAGCGCACGGacaaca                                                                          >  2:323719/1‑250 (MQ=255)                                                                                                                                                                              cGAAGTTCCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGGCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGGACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGTCAACAATGACCGAc                                                                 <  2:254076/251‑1 (MQ=255)                                                                                                                                                                                      cccTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGGCAACAATGACCGACATCGCATa                                                         <  1:175162/251‑1 (MQ=255)                                                                                                                                                                                       ccTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATa                                                         >  2:144526/1‑250 (MQ=255)                                                                                                                                                                                          gACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATcc                                                     <  1:402809/251‑1 (MQ=255)                                                                                                                                                                                          gACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATcc                                                     <  1:571542/251‑1 (MQ=255)                                                                                                                                                                                             cGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGa                                                  <  2:420863/251‑1 (MQ=255)                                                                                                                                                                                                cTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGAt                                                 >  1:158502/1‑249 (MQ=255)                                                                                                                                                                                                  aCCAGCAGATAACGCAGCGAGAATGGCTGCGCCAGGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAGTCCAGATa                                                <  1:3719/248‑1 (MQ=255)                                                                                                                                                                                                      gCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATTGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCAGAATCCAGATAGGagctc                                         <  2:523999/251‑1 (MQ=255)                                                                                                                                                                                                        atatAACGCAGCGAGAAGGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTTCGTCACCAACGACACGGGCAGATGTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctctt                                       <  2:522932/251‑1 (MQ=255)                                                                                                                                                                                                                cAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTTTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGTACATCGGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCa                                                    <  2:372071/230‑1 (MQ=255)                                                                                                                                                                                                                cAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTAACGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCa                                                    >  1:372071/1‑230 (MQ=255)                                                                                                                                                                                                                    gagaATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTc                           <  1:40993/251‑1 (MQ=255)                                                                                                                                                                                                                    gagaATGGCTGCGCCATGAGGTCGCTAGTGTTCTAGACCGCCGAGTCACCATGGGGTTGGTATTTCCCGATTACGTCACCAACGACACGGACAGATTTTTTCTAGGCTTTGTTCCAGTCATTGCCCCGTACGTTCATGGCGTAAAGTACGCGACGGTGTAACGGCTTCCGGCCATCTCGGTCATCTGGCAGCGCACGGCCAACAATGACCGCCATCCCATACTCCAGATAGGAGCTCTTCCGCTCTTCCTc                           >  2:559180/1‑251 (MQ=255)                                                                                                                                                                                                                          ggCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGTGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTATGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGtt                     <  2:415931/251‑1 (MQ=255)                                                                                                                                                                                                                          ggCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGtt                     >  2:513166/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGgt              <  2:401801/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGgtgt            <  2:490413/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGATCGGGTTATAGACCGCCGAGTCGCCATGGGGATGGTATTTACCGATTCCGTCACCAACGACACGGGCAGATTTTTGATAGGCTTTGTTCCAGTCATTGCCTAGTGCGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTGCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtct     <  2:449704/251‑1 (MQ=255)                                                                                                                                                                                                                                           gATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtctc    <  1:438125/251‑1 (MQ=255)                                                                                                                                                                                                                                            aTCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCGCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtctct   <  1:79894/251‑1 (MQ=255)                                                                                                                                                                                                                                             tCGTGTTATAAACCGCCGAGTCACCATGGGGATGGTGTTTCCCGATTACGTCACCCACGAAACGGGCAGATTTTTTATAGGCTTTGTTCCAGACATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGAACCGCACACAATGACCGACATCGCATAATCCAGCTAGGAGCTCTTCAGCGCTTCCTCAGTGTTGACCGGTGTAATTtctctc  >  2:218904/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     CAGCAGGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTC  >  NC_000913/2338920‑2339406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑