Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,209	A→G	W197R (TGG→CGG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,209	0	A	G	100.0%	200.6 / NA	56	W197R (TGG→CGG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/28);  total (28/28)

CCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGC  >  NC_000913/2328966‑2329453                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ccGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCCACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTc                                                                                                                                                                                                                                               >  1:127016/1‑251 (MQ=255)      ttACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTACACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGATCCGGATTTTCACCCg                                                                                                                                                                                                                                          >  2:76540/2‑251 (MQ=255)                      tgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAACACCCGATTAAGCACCGTCGCATAACGGGTTAGCACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGc                                                                                                                                                                                                                          >  2:293281/1‑251 (MQ=255)                          tttCTTCAGCGGATAGCGGGCAAACAGCTCCCCCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTGCCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTCCCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTGCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATg                                                                                                                                                                                                                       <  2:229805/250‑1 (MQ=255)                              gtcAGCGGAGAGCGGGCAATCCGCTCCACCTCTTCACATTGCCCACTGGCAACCTGCGCCGTCACTGTCAAAACCCGATTATGCACCTACGCTTAACCGGTTAGGACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTTTTCCTTCGGTTAACCAGCCAGCAAAACCACCCTGTCGGATTTCCTGTTTATCATCTGCAAACCATCGCCGGGTTTTCCCCCGTATCCGACTACCGAGCATAGAtcc                                                                                                                                                                                                                  <  2:519434/250‑3 (MQ=255)                               tcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATGGCCCACTGGCAACCGGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGGTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCAGCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGc                                                                                                                                                                                                                  <  2:401945/250‑1 (MQ=255)                                cAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGATTACCGAGCATGGATGCgc                                                                                                                                                                                                                >  2:257047/1‑250 (MQ=255)                                cAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCg                                                                                                                                                                                                                 >  1:436468/1‑250 (MQ=255)                                      aTAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTAGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGCTTTCACCCGGACCCGCCTACCGCGCATGGATGCGaccc                                                                                                                                                                                                             >  2:32020/1‑246 (MQ=255)                                       tAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAt                                                                                                                                                                                                         >  2:83841/1‑251 (MQ=255)                                         gCGGGCAACCAGCCCCACCTCTACACATTGCCCACTGGCAACCGGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAttt                                                                                                                                                                                                       <  2:315172/251‑1 (MQ=255)                                              cAAACAGCTCCACCCCTACACATGGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCGCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGcc                                                                                                                                                                                                  <  2:212236/251‑1 (MQ=255)                                                    gCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTcc                                                                                                                                                                                            >  1:95072/1‑251 (MQ=255)                                                      tCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTCCCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTcc                                                                                                                                                                                            >  2:104433/1‑249 (MQ=255)                                                      cccACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGGACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAg                                                                                                                                                                                          <  2:500695/250‑1 (MQ=255)                                                          ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCTCCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcacc                                                                                                                                                                                      <  1:348992/251‑1 (MQ=255)                                                          ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcacc                                                                                                                                                                                      >  2:398232/1‑251 (MQ=255)                                                              tACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCGAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACAt                                                                                                                                                                                  >  2:151864/1‑251 (MQ=255)                                                               acacATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGTATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACTCCGATTTTCGCCTCGTCCAGCACCACATc                                                                                                                                                                                 >  1:195365/1‑251 (MQ=255)                                                                  caTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATcc                                                                                                                                                                                >  1:294326/1‑249 (MQ=255)                                                                   aTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGt                                                                                                                                                                             <  1:333164/251‑1 (MQ=255)                                                                     tGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGGTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACAGCCAGTa                                                                                                                                                                            <  2:245713/250‑1 (MQ=255)                                                                              gCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGGCTTCCTg                                                                                                                                                                  <  2:370965/251‑1 (MQ=255)                                                                                aaCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAg                                                                                                                                                                <  1:104433/251‑1 (MQ=255)                                                                                                 gCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGt                                                                                                                                               <  1:76666/251‑1 (MQ=255)                                                                                                  cAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACCTCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGt                                                                                                                                               >  2:460815/1‑250 (MQ=255)                                                                                                     aaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACg                                                                                                                                           >  2:444131/1‑251 (MQ=255)                                                                                                       cccGATTAGGCGCCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTc                                                                                                                                         <  1:340324/251‑1 (MQ=255)                                                                                                         cGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCAAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCAAGCAAAACCACCCTGAAGGATTTCATGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAATAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGc                                                                                                                                       >  1:572486/1‑251 (MQ=255)                                                                                                          gATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGATTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCCCATCCAGTAAGACTTCCTGCACTTTGTTTTGCGCGGGTAACGTCGCt                                                                                                                                      >  1:110197/1‑251 (MQ=255)                                                                                                              aaccACCGGCGCATAACGGTTGATTACCGTTTTGCTGGGGCGACTTCCAGTAACCCACAGCGGTGTACCATCGGATAACCAGCCAGCAAAACCACCCTTACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCTCCCGTACCCGACTACCGAGCATGTATGCGTCACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGTAGGTAACGTCGTTAATg                                                                                                                                  <  2:280675/248‑1 (MQ=255)                                                                                                                gCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGa                                                                                                                                 >  2:63146/1‑250 (MQ=255)                                                                                                                  aCCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATc                                                                                                                              <  1:532516/251‑1 (MQ=255)                                                                                                                   ccGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATc                                                                                                                              >  1:386197/1‑250 (MQ=255)                                                                                                                       cGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAagc                                                                                                                          <  1:235108/250‑1 (MQ=255)                                                                                                                          aTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCg                                                                                                                      >  2:419593/1‑251 (MQ=255)                                                                                                                                ggTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTa                                                                                                                 >  2:336444/1‑250 (MQ=255)                                                                                                                                 gTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCGAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAAc                                                                                                               <  1:151864/251‑1 (MQ=255)                                                                                                                                          gTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCg                                                                                                       >  1:377270/1‑250 (MQ=255)                                                                                                                                             ttGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATAGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGt                                                                                                   >  2:369594/1‑251 (MQ=255)                                                                                                                                                  ggTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCCCCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCt                                                                                               <  2:95072/250‑1 (MQ=255)                                                                                                                                                      ccACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCtt                                                                                          <  1:398232/251‑1 (MQ=255)                                                                                                                                                                      acaGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGAc                                                                          >  1:223136/1‑251 (MQ=255)                                                                                                                                                                                                ccagcAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTg                                                >  2:238975/1‑251 (MQ=255)                                                                                                                                                                                                   gcAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTg                                             <  1:280972/251‑1 (MQ=255)                                                                                                                                                                                                    aaaaaCCACCCGGACGGATTTCATGTTGATCAACCGCAAACCTGCTCCGTGTTTTCACCCGGACCCGACTACCGAGTATGGATGCGAAACCGATTTTCTCCTCGTCCAGCACCACATCCAGTAAGACTGCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATTAATCAAGCAGCTATTTAACCGTTTCCGAGGTTGCTGGCTTCAGCGTAGTCAGAGACGCTAACCACACAGTTGCCTTACgtggttgc                                            <  2:258899/250‑8 (MQ=255)                                                                                                                                                                                                     aaaaCCGCCCGGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGcc                                           <  2:461571/251‑1 (MQ=255)                                                                                                                                                                                                     aaaaCCCCCCTGACGGATTTCCGGTTTATCATCGGCAAACCAGCTCCGGGGTTTCACCCGTGCCCGACTACCGAGCATTGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCATAGACGCTAACCACGCAGGTGCCTGACGCTGTTGcc                                           <  2:513869/251‑1 (MQ=255)                                                                                                                                                                                                      aaaCCACCCTGACGGATTTCCTGGTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCTAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTGTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCa                                          <  2:182625/251‑1 (MQ=255)                                                                                                                                                                                                                       ttCCTGTTTATCATCGGCAAACCAGCTCCGGGATTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCAATTATTGCCCCCACACAt                         >  1:110184/1‑251 (MQ=255)                                                                                                                                                                                                                            gTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTg                      <  1:319456/249‑1 (MQ=255)                                                                                                                                                                                                                               tATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGcc                  >  1:3924/1‑250 (MQ=255)                                                                                                                                                                                                                                  catcGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGATTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAata              <  1:257047/251‑1 (MQ=255)                                                                                                                                                                                                                                  catcGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGt                                                                                                                                               >  2:485374/1‑122 (MQ=255)                                                                                                                                                                                                                                  catcGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGt                                                                                                                                               <  1:485374/122‑1 (MQ=255)                                                                                                                                                                                                                                              aGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACTCCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTgc  <  2:195365/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGC  >  NC_000913/2328966‑2329453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑