Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	100.0%	143.0 / NA	44	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/22);  total (22/22)

TTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCG  >  NC_000913/2173205‑2173685                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              ttAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCg                                                                                                                                                                                                                                      >  2:486577/1‑251 (MQ=255)        gCGGAACAGATTCCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGACCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCg                                                                                                                                                                                                                               <  1:281034/251‑1 (MQ=255)         ccgAACGGATTTCCACGCCCCACGGCGCCCGCAATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGAAGCCCCGGATTACCCGGCACACAAACATCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGATGGCCGATTC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGATCTCCTGACCGCCGAACTTCGCCGGTAAACGACTACGAGCTTGCTGAGCGATGGGCGt                                                                                                                                                                                                                              <  2:468538/249‑1 (MQ=255)          ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGtt                                                                                                                                                                                                                             >  1:450736/1‑251 (MQ=255)           gAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTa                                                                                                                                                                                                                            <  1:21842/251‑1 (MQ=255)             aCAGAGTTCCACGAGGCACGGGTACCGCCATCGCCACGAAGAAGCCGATGGTGTCAAGATCGCAAAACGGCAGCACTTGATAACCCGTCACACAAACAGCAATTAAAATGTTGAGTGGGA‑TAAAAATCAGGCTTTCCGATTC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACCCCTGTCCGCCGAACTTCGCCTGTCAACGACTACGAGCTTGCTTAGCGATGGGCGTTaaa                                                                                                                                                                                                                          <  1:62354/251‑1 (MQ=255)                 aTTTCCCCGATCCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAccat                                                                                                                                                                                                                      <  1:399445/251‑1 (MQ=255)                 aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCACTTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTCTGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCCGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGAGCTTGCTTAGCGATGGGCGTTAACccat                                                                                                                                                                                                                      >  2:586301/1‑251 (MQ=255)                     ccACGATGAGCGGCCACCGCCACCGCCACGAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAGGGTGAGTGGGA‑TAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAccatccat                                                                                                                                                                                                                  <  2:495173/251‑1 (MQ=255)                       ccgaggcacgccgactcccctCGCTACGAAGAAGCCGAGGGGGGCAAGATCGCCAAACGGCAGCCCCTGATTACCCGGCACACAAACAGAAATAACAATGGCGAGTGGAA‑TAAAAATCAGGCTTGCAGATAC‑‑CCCCGCCGTATGCCCCAGCATCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatga                                                                                                                                                                                                                <  2:416835/231‑1 (MQ=255)                                    aCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAc                                                                                                                                                                                                   >  1:267270/1‑251 (MQ=255)                                             gCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt                                                                                                                                                                                          >  1:288570/1‑251 (MQ=255)                                                aagaagaaGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGACACACAAACAGCAATAAAAATGGTGAGTGGGA‑TAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTag                                                                                                                                                                                       <  2:288570/249‑1 (MQ=255)                                                     aaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCGGATTACCCGGCACACAAACAGAAAGAAAAATGGGGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAgcagca                                                                                                                                                                                  <  1:82635/250‑1 (MQ=255)                                                        gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATc                                                                                                                                                                                >  1:250684/1‑250 (MQ=255)                                                         ccGATGGTGCCAAGACCGCCAAACGGACGCCCCTGATTACCCGGCCCACAAACAGCAAGTAAAAGGGTGTGTGGGA‑TAAAAAGCAGGCTTGCCGATAC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCAc                                                                                                                                                                              <  1:47416/251‑1 (MQ=255)                                                           gaggggggCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAAGGGGGAGGGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTg                                                                                                                                                                            <  2:267270/245‑1 (MQ=255)                                                              ggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgccg                                                                                                                                                                         >  1:490560/1‑251 (MQ=255)                                                                 ggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGccgccg                                                                                                                                                                      >  1:297805/1‑251 (MQ=255)                                                                     aGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtttt                                                                                                                                                                  >  1:460829/1‑251 (MQ=255)                                                                       accGCCAAACGGCAGCCCCTGATTACCCGTCACACAAACAGCAATAAAAAGGGGGAGGGGGA‑AAAAAATAAGGCTGCCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTa                                                                                                                                                                <  2:18676/249‑1 (MQ=255)                                                                          gCCAAACGGCAGAACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCCCTGCCGCCGTTTTTACCg                                                                                                                                                             >  2:44341/1‑251 (MQ=255)                                                                            cAAACGGCAGCACCTGATTACCCGGCACACAAACCGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                                                                           >  2:511187/1‑251 (MQ=255)                                                                                            attccccGGCACACAAACAGCAATTAAAAGGGTGA‑TGGGAATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCtt                                                                                                                                           <  1:368642/247‑1 (MQ=255)                                                                                                     cacacaAACAGCCATTAACATGGCGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGCT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATAACCCGTGGCATTAGCCGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGt                                                                                                                                  >  2:224005/1‑251 (MQ=255)                                                                                                        acaAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAc                                                                                                                               >  1:526787/1‑251 (MQ=255)                                                                                                          aaaCAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAccc                                                                                                                             >  1:458386/1‑251 (MQ=255)                                                                                                          aaaCAGCAATAAAAAGGGTGGGGGGGA‑AAAAAACCAGGCTGGCCGAAAC‑‑CCCCGCCGTATGTCCCAGCAGAAACGCCGGAT‑CAAGGCCAATCAGGAACCCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCAGCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAccc                                                                                                                             <  2:303396/251‑1 (MQ=255)                                                                                                             cAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTGGACATCGTAACCcgcg                                                                                                                          >  1:96380/1‑251 (MQ=255)                                                                                                                  aTTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCACTACACCTTTGACATCGTAACCCGCGAGGAt                                                                                                                     >  2:224602/1‑251 (MQ=255)                                                                                                                  aTTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGAt                                                                                                                     >  1:421324/1‑251 (MQ=255)                                                                                                                              aGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                                                                                           >  2:428099/1‑249 (MQ=255)                                                                                                                               gTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGc                                                                                                        >  2:188440/1‑251 (MQ=255)                                                                                                                                             acacgggctgccaacacccacggcgTATGTCCCAGCAGCAACGCCGGAAACAAG‑CCAAACAGGAACCCCTGACCGCCGACATTCGCCTGTAAACGGCTTGGGGGTTGCTTAGCGATTGGCGTTAAACCAACCATGATGGGTTTAATCCCCCCGGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTTCAATACCCCCTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaa                                                                                             <  2:96380/228‑1 (MQ=255)                                                                                                                                                    ttgccgaacccaccgccgTATGTCCAAGCAGCACCGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCGGTAAACGACTACGAGCTTGCTTAGCGATGGGCGATAAACCAACCATGAGGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCAc                                                                                       <  1:586301/238‑1 (MQ=255)                                                                                                                                                         aatcccaccgccgTATGTCCCAGCGGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTGGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc                                                                                 <  1:188440/244‑1 (MQ=255)                                                                                                                                                                                  cgaaaccccgacaaaaGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGGAAACGACTACGAGCTTGCTTAGCGATCGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCGTCACGGCCGCCGTTTTTACCGCCAGCTGCAATACTCCTTTGACATCGTAACCCGCTAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCTCCAAATTGACCAAAttt                                                         <  2:559286/237‑1 (MQ=255)                                                                                                                                                                                   cacccccGGAT‑CAAGTCCAATCAGGAATTCCGGACCGCCGAACCTCGCCTGTAAACGACTACGAGCTTGCTGAGCGATGGGCGTTAAACCATCCATTATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTCGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGTCTCGGCAAATGGACCAAATTTgc                                                       <  2:373035/246‑1 (MQ=255)                                                                                                                                                                                                aaGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcgt                                           <  1:550499/251‑1 (MQ=255)                                                                                                                                                                                                   gCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCgg                                        >  1:203722/1‑251 (MQ=255)                                                                                                                                                                                                                                 cGCCTGTAAACGACTACGATCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCTTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCTCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTggg           <  1:564875/250‑1 (MQ=255)                                                                                                                                                                                                                                     tGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGCTAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTTCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGCtttt      >  2:395406/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTAAACGCATACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGCCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTtc     >  1:440586/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaCGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGTGATTTTCTCg  <  1:156982/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              TTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGA‑TAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGAT‑CAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCG  >  NC_000913/2173205‑2173685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑