Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,137,543	G→A	intergenic (‑300/‑359)	wza ← / → yegH	outer membrane polysaccharide export protein Wza/inner membrane protein YegH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,137,543	0	G	A	100.0%	98.7 / NA	30	intergenic (‑300/‑359)	wza/yegH	outer membrane polysaccharide export protein Wza/inner membrane protein YegH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/17);  total (13/17)

ATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAGGGATAATG  >  NC_000913/2137303‑2137781                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               aTGCACATTTACCTTGCATTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTAt                                                                                                                                                                                                                                      <  2:324498/251‑1 (MQ=255)     ccaTTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTa                                                                                                                                                                                                                                  <  1:146131/250‑1 (MQ=255)           aCCTTGCAGTTAATTGAATAAAAGTTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGCAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGa                                                                                                                                                                                                                             >  2:328306/1‑250 (MQ=255)                   gTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCt                                                                                                                                                                                                                    >  1:470486/1‑251 (MQ=255)                   gTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAAGCTATTGCCAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTCGTTTAATTTTTCAAACCCTCTCAGCATGCAGTCGTTGTTGAGAAAGGGTTATTACGGAAATTACCTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCt                                                                                                                                                                                                                    >  2:559859/1‑251 (MQ=255)                          gAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCaat                                                                                                                                                                                                             >  2:192700/1‑251 (MQ=255)                              aaaaaTTTCCCTGGCATAAGTCCTAACAAAATTGATTTCATCAGCAGGCTATTGACAGAATAATTCAGACTG‑GTATTTCAGGCATCCAGACACGCTACCGCCCCAGGCTTTTTAGCTACCAATACAATGATTTAGTTTAATTTTTCACACACTCTCAGCATGCAGTCGATGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTACTTGCATATTGGCTTTCCAATAATg                                                                                                                                                                                                         >  2:550901/1‑251 (MQ=255)                                 aaTTTAACTGGCATCAGCCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACATAATAATTCAGACTGTGT‑TTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCag                                                                                                                                                                                                      <  1:520158/251‑1 (MQ=255)                                       aCTGGCATCAGTCATAAAAAAATTGATTTCATCCGCAGGCTATTGACAGCATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGCCGTTGATGAGAAAGGGTTATTCCGGAAATTAACTTCCGAATATAAAGGGACATTATGGTAATTGAATATTGGCTTTCCATTAATGCAggagga                                                                                                                                                                                                 >  2:405623/1‑250 (MQ=255)                                                    cTAAAAAAATTGATTTCATCCGCAGGCTATTAACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTACTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTa                                                                                                                                                                                    >  2:399603/1‑250 (MQ=255)                                                       aaaaaaTTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACgg                                                                                                                                                                                >  1:534754/1‑251 (MQ=255)                                                             ttGATTTCATCCGCAGACTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCACCTGGCTTTTTAGCTACCAATAAACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGATATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGc                                                                                                                                                                          >  2:448758/1‑251 (MQ=255)                                                               gATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGGGTTACAGCTAACGGAATAGCa                                                                                                                                                                         <  1:192700/250‑1 (MQ=255)                                                                aTTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAg                                                                                                                                                                        <  1:311821/250‑1 (MQ=255)                                                                                aTTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCgg                                                                                                                                                       <  2:33750/251‑1 (MQ=255)                                                                                aTTGACAGAATAATTCAGACTG‑GTCTTTCAGGAATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTc                                                                                                                                                         <  1:428895/249‑1 (MQ=255)                                                                                                  aCTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAg                                                                                                                                     <  1:278978/251‑1 (MQ=255)                                                                                                             cAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTa                                                                                                                           <  2:534754/251‑1 (MQ=255)                                                                                                                       gacccGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGTAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTc                                                                                                                 <  1:520/247‑1 (MQ=255)                                                                                                                       gACACGCTCCCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTc                                                                                                                 <  2:401003/251‑1 (MQ=255)                                                                                                                                                    aCCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGtt                                                                                    >  2:102068/1‑251 (MQ=255)                                                                                                                                                        aTACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACa                                                                                <  1:586121/251‑1 (MQ=255)                                                                                                                                                            acTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACa                                                                                >  1:196142/1‑247 (MQ=255)                                                                                                                                                            acTGAGTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGGCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACa                                                                                <  2:196142/247‑1 (MQ=255)                                                                                                                                                                        tAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGaa                                                                                                                                                                                                >  2:440403/1‑123 (MQ=255)                                                                                                                                                                        tAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGaa                                                                                                                                                                                                <  1:440403/123‑1 (MQ=255)                                                                                                                                                                                        cgcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTTTCATTCAATATTGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATAtt                                                <  1:27476/249‑1 (MQ=255)                                                                                                                                                                                                                aaGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTAc                        <  1:525744/251‑1 (MQ=255)                                                                                                                                                                                                                  gggTTATTACGGAAATTAACTTCCGAATATATAGTGACATTATTGTAATTTAATATTGTCTTACCAATAATTCATGAGGAAGTGTTGCAGCTATCTGAATAGCAGTCAAGATAACAATTCGTTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAAAATGGTTTTTATGAGTTTCTTTATGTTGACAATTTTTAATATCGTGTCTCCACTTGCGACAATTCTTATATAATGTATTATTATTACCa                      <  2:333997/251‑1 (MQ=255)                                                                                                                                                                                                                                      ttCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAGGGATAAtg  >  1:177390/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               ATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTG‑GTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAGGGATAATG  >  NC_000913/2137303‑2137781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑