| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,086,634 | G→A | A143A (GCC→GCT) | plaP ← | putrescine:H(+) symporter PlaP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,086,634 | 0 | G | A | 95.2% | 200.6 / NA | 65 | A143A (GCC→GCT) | plaP | putrescine:H(+) symporter PlaP |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (28/32); minor base C (3/0); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
CCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAA > NC_000913/2086390‑2086872 | ccGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTc > 1:154522/1‑251 (MQ=255) cGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTc > 1:30631/1‑250 (MQ=255) gATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGccac < 2:419277/249‑1 (MQ=255) gCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAgcg > 1:188219/1‑251 (MQ=255) gCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACcaca > 1:172104/1‑250 (MQ=255) aaaGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGCCGAGTGCTCGCCAACGTACCAGCGCCTTCACCTTCAAATACGCCATAAAAAACCATGCCCAGAATCACCACGATCAGCACTACCTGCAACACTGCGATTACCGTGATGACGTTCGCTACGAATTTCAGCCGACGCAGGTTAAAAGTGGACATAAAGGCCACCAGCGCCACCACAAAcctac > 1:236036/1‑247 (MQ=255) aaaGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATcc > 1:454770/1‑251 (MQ=255) gaAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACAGGCGCATCGCCAGACCAGAATGGAAGAGTGCACGCCAGCGTACCAGCGCCTTAACCTTCAAATACGCCATAAACAACCATGCCCAGAAGCACCGCGAGCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGGCATAAAGGCCACCAGCGCCACCACAAACATCCACgatg < 2:395088/251‑1 (MQ=255) acaGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatgggatg < 1:356438/251‑1 (MQ=255) caGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCAGCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatgggatgg < 2:188219/251‑1 (MQ=255) gATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGa > 2:277727/1‑249 (MQ=255) tGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGc < 1:47452/250‑1 (MQ=255) cGGTAAGCATCTGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCATTCACCTGCCAATACTCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTTCCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGAAGGTGAAAAGCGGTCACAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCCCCAGAGcg < 2:206797/251‑2 (MQ=255) gttAATCATCGGGATAACATGCGCAGCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTTTGGAAGTACGCTACGGATTTCATACTACGCATGTTAAAAGCGGTCAGAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCtt < 2:567845/249‑1 (MQ=255) gTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCAATAAACAACAATGCCCAGAATCACCGCGATCAGCAATACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTCAATGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCCCCAGAGCTTc > 2:329501/1‑251 (MQ=255) aaTCATAGGGAGAACAGGCGAATCGGCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCTCCGCGATCAGCCCTCCCTGCTACACGACGATTACAGTTTTGGAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCaa < 1:267537/251‑1 (MQ=255) cGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAAt > 2:412639/1‑248 (MQ=255) cGGGATAACATGCGCATCGCCAGACCAGAACGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGAGGGGATGGAAGGCACCAGATCTTCAAAATAGa < 2:50050/251‑1 (MQ=255) gggctcaCATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCCGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAAt > 2:390356/7‑247 (MQ=255) aCATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGc < 1:269745/251‑1 (MQ=255) gaTGCGCATCGCCATACCAGAAATGACGAATGCTCTCCAGCGTACCAGCGCCTTCCCCTTCAAATACTCCATAAAAAACCAGGCCCAGAATCACCTCGACCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTCCGGATTTCAGACTACGCAGGTTGAGGTCGGTCATACAGGCCACCAGCGCCACCACAAACATCCCCGCTGGGATGGGAGGCACCCGAGCTACAAAATAGACTTTCGcc > 2:517753/2‑251 (MQ=255) aTGCGCCTCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTGCCCCTTCAAATACGCCAGAAACAACCAGGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTTCGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCTCCAGATCTTCAAAATAGATTTTCGCCa < 2:183755/251‑1 (MQ=255) gcgcATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATATATTTGCGCCAGt < 2:284449/251‑1 (MQ=255) gcgcATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGt > 1:194081/1‑251 (MQ=255) cgcATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTa < 1:446282/251‑1 (MQ=255) gcATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAg < 1:9681/251‑1 (MQ=255) cGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATg < 1:136852/251‑1 (MQ=255) ccagaccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGtt < 2:299490/251‑1 (MQ=255) cagaccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCAAAAACAACAAAGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAACGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTg > 1:75698/1‑251 (MQ=255) agaccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGa < 1:412639/251‑1 (MQ=255) ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCGTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGTAAGGCACCAGAGCTTCAAAATAGAGTTGCGCCAGTAGAATTTTGatca < 2:373668/251‑1 (MQ=255) gAGTGCTCGCCAGCGTCCCAGCCCGATCACCTTCAAATACGCCATAACCAACCATGCCCACGATCACCGCGATCAGCAACACCCGCAACACGACGATTACAGTGTGGAAGCTCGCTACGGATTACAGACTACGCAGGTTAAAAGCGGTCAGAAAGTCCACCAGCGCCACCCAAAACATCCAGGATGGGATGGAAGGCACCAGAGCTTCAAAAGAGATTTTCGCAAGGAGAATGTTGATCATCGGCGCGAAc < 2:289300/251‑1 (MQ=255) gCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTc > 1:132133/1‑251 (MQ=255) cagagaaCCAGCGCCTTCACCCTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCATCACTCCCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACTTCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTCGCGCCAGTAGAATGTTGATCATCGTCGCGAACAGATAGTCGa < 2:153454/245‑1 (MQ=255) cAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGCGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGCTGGGATGGAAGGCACAAGAGCTTCAAACTAGGTTTTCGCCAGTAGAATGTTGATCATCGTCGCGAACAGAAAGTCg > 2:45574/1‑250 (MQ=255) cttcaccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCa > 2:217854/1‑250 (MQ=255) ccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCa < 1:511236/235‑1 (MQ=255) ccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCa > 2:511236/1‑235 (MQ=255) tcaAATACGCCATAACCCACCATGCCCAGAATCACCGCGATCAGCACTAACTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGCTTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCAt > 1:201831/1‑251 (MQ=255) cATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGATACGGATTTCAGACTACGCAGGTTCAAAGCGGTCATAAAGGCCACCAGCGGCACCACAAAAATCCACGCTGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGCAGACCAACCCACCATAAAGCCGAc > 1:585067/1‑250 (MQ=255) aaaCAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc < 1:507221/251‑1 (MQ=255) acCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCt < 1:37995/251‑1 (MQ=255) aGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTGCGCTACGGATGTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGAttt < 1:79704/251‑1 (MQ=255) cAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAgtgt < 1:217854/251‑1 (MQ=255) ccTACCTGCAACACGTAGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCCCCACAAACATCCACGATGGGATGGAAGGCTCCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCTCCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGc < 1:498327/250‑1 (MQ=255) aCTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGc > 1:239194/1‑251 (MQ=255) aCCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCaga < 1:277727/251‑1 (MQ=255) ccTGCAACAAGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGGTAAAAGCGGTCATAAAGGCCACAAGCGCCACCACAAACATCCACGATGGGATGGACGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAAAAGATAGTCTAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGGAAGTGTATGCag > 2:209768/1‑249 (MQ=255) cTGCAACACGACGATTACAGTGTTGAAGTTCAATACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACAAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGc > 1:515426/1‑251 (MQ=255) cgacgaTTACAGTGTTTAAGTGCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCTCCAGCGCCACCACAAACATCCACGAGGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATTTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAATACCAACCTCCCATAAAGCAGACAGTCGGTTTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGaa < 1:192491/251‑1 (MQ=255) cgacgaTTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGAc < 1:474959/202‑1 (MQ=255) cgacgaTTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGAc > 2:474959/1‑202 (MQ=255) cgacgaTTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGCGTATGCAGAGCCAGCAGa > 2:350363/1‑250 (MQ=255) acgaTTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAgg > 2:398384/1‑251 (MQ=255) tACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGATCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATATTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTa < 1:554249/205‑1 (MQ=255) tACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAgc > 1:107033/1‑251 (MQ=255) tACAGAGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGACACCACAAACATCCACGATGGGATGGACGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTa > 2:554249/1‑205 (MQ=255) cAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAACGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAgcgg > 1:224078/1‑251 (MQ=255) ggATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGc > 1:224735/1‑251 (MQ=255) ggATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGc > 1:182302/1‑251 (MQ=255) cTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaaga > 1:87680/1‑143 (MQ=255) cTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaaga < 2:87680/143‑1 (MQ=255) tACGCAGGTTAAACGCGGTCATAAAGGCCACCAGCGCCACCACACACATCCAAGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGACTGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGt > 2:269835/1‑250 (MQ=255) tACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGt > 1:280851/1‑250 (MQ=255) aCGCAGGTTAAAAGCGGTCATAAAGGCCCCCAGCGCCACCACAAACATCCACGATGGGATGGAAGTCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGGCGAGCAGAGAAGACCAACCCACCATAAAGCCTACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTaa < 1:371542/251‑1 (MQ=255) | CCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAA > NC_000913/2086390‑2086872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |