Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,085,319	G→A	A69A (GCC→GCT)	tsuA ←	thiosulfate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,085,319	0	G	A	100.0%	92.7 / NA	28	A69A (GCC→GCT)	tsuA	thiosulfate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/11);  total (17/11)

ATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCGGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTAT  >  NC_000913/2085076‑2085563                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aTTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGt                                                                                                                                                                                                                                               >  1:13812/1‑251 (MQ=255)  atAAAAGTCTCAGCAAAAGAGTTATGTTCAGTACTGTAGTGCTGCAAGTTTTGATTTAAACCCCTGGCATGGGGGGAACGCATCCCCGCACTCATCACCATATAAGTGAAAAGCGCTATCCAACTTCCGATCAAGCCCACACCCGCGCGATACCAGGTCCCGGTGGC‑CCATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTa                                                                                                                                                                                                                                              <  1:579340/250‑1 (MQ=255)                   agagTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGCTCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCt                                                                                                                                                                                                                             >  1:340135/1‑251 (MQ=255)                                                   tttGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAACCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATACCAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTAcc                                                                                                                                                                                             >  2:586141/1‑251 (MQ=255)                                                    ttGATTTAAACCACTGGCATGTGGAGAACGCATCACCCCCCTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCCCCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTAcc                                                                                                                                                                                             <  1:69766/250‑1 (MQ=255)                                                         ttAAACCCCTGGCATGTGGAGAACGCACCCCCGCACCCATCACCATATAAGTGAAAAGCGCGATCCACCTGCCGATCAACCCCTCACCCGCGCGATACCATGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAAt                                                                                                                                                                                        <  1:83639/250‑1 (MQ=255)                                                                aCTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAACTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATc                                                                                                                                                                                 >  2:435563/1‑250 (MQ=255)                                                                     cATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCa                                                                                                                                                                             >  1:440596/1‑249 (MQ=255)                                                                                               atcaCAATATAAGTGAAAAGCACGATCCAACTGCCGATCAATCTCTCACCCGCGCGATACCAGGTCCCGGCGGC‑ACATCCACCCGCCAGAACACTTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTACGTCAGTCGGCCCCCCTGAATTAACGCAAACACCCCCACGCTTTTTTCCGCAATTGCAATCAGCAAGTCGAAAAACATCAGATTATTTTTCAc                                                                                                                                                 >  2:404946/1‑251 (MQ=255)                                                                                                ccaCCATATATGTGAAAAGCGCAATCCAACGGCCGATCAATCCCTCCCCCGCGCGAGACCAGGTCCCG‑TGGCCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACg                                                                                                                                                <  1:374231/250‑1 (MQ=255)                                                                                                    cATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGACCAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGGAAAACATGCGATTATTTTTCACGatat                                                                                                                                            >  1:411929/1‑251 (MQ=255)                                                                                                      tataAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCttt                                                                                                                                                                                                  >  1:426297/1‑195 (MQ=255)                                                                                                            ggAAAAGCGCGATCCAACTGCCGATCAATCCCTCCCCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATcg                                                                                                                                    <  1:419743/250‑1 (MQ=255)                                                                                                                 aGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa                                                                                                                                >  1:162177/1‑250 (MQ=255)                                                                                                                   cgcgATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGACAac                                                                                                                             >  1:45009/1‑251 (MQ=255)                                                                                                                   cgcgATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAac                                                                                                                             >  1:128629/1‑251 (MQ=255)                                                                                                                   cccgacccaccTGCCGACCAATCCCCCCCCCGCGCGATACCAGGTCCCGGGGGC‑ACATCCCCCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAac                                                                                                                             <  1:99941/241‑1 (MQ=255)                                                                                                                       aTCCAACAGCCGATCAATCCCTCACCCCCGCGATACCAGGTCCCGGTGCC‑ACATCCACCCGCCAGAAAAATTCCCAGCCCGAAGATATACCCTCCTATAACAGTACCAAGCCACGGGAACGCCCCAGTTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAaccacc                                                                                                                         <  1:578652/251‑1 (MQ=255)                                                                                                                          cAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGt                                                                                                                      >  1:87401/1‑251 (MQ=255)                                                                                                                           aaCTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGCATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc                                                                                                                     >  1:497919/1‑251 (MQ=255)                                                                                                                                      aaTCCCTCCCCCCGGCGATACCAGCTCCCTGTGCA‑ACACCCCCCCGCCAGAACAATTCCCAGCCCGAAGATATCCCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTGCACGATATACATATCGCGAAAACCACCTGTCAGTCAAAAACg                                                                                                          <  2:549745/251‑1 (MQ=255)                                                                                                                                        tCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGcc                                                                                                        >  1:293680/1‑251 (MQ=255)                                                                                                                                                          accagacaccggtagaacaTCCACACGCCAGCACAATTGCCAGCCCGAAGAAATACCCAACAATAACAGTACCAAACAACGGGAACGCCCCAGCTTCGACAGACAGTCGACCCGCCTGAATTAACGCCAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCACGCACAAACGCCCACGCTGCATGACAAAt                                                                                       >  2:390856/17‑251 (MQ=255)                                                                                                                                                                    gTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGc                                                                            >  1:475395/1‑251 (MQ=255)                                                                                                                                                                                                            atatccacACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATaaa                                     <  2:157092/244‑1 (MQ=255)                                                                                                                                                                                                             aataCCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAAGCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAaat                                    <  2:90307/250‑1 (MQ=255)                                                                                                                                                                                                                 cccACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAACACCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACAACCTGTCAGGCAAAAACGCCCAAGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCACTTAATATCATTGAAAACATAaattaat                                >  1:280124/1‑251 (MQ=255)                                                                                                                                                                                                                                               cGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAattat  <  1:586141/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGC‑ACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCGGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTAT  >  NC_000913/2085076‑2085563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑