BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	281,291	G→A	I231I (ATC→ATT)	yagA ←	integrase core domain‑containing protein YagA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	281,291	0	G	A	98.5%	413.0 / NA	132	I231I (ATC→ATT)	yagA	integrase core domain‑containing protein YagA
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (71/59); minor base C (2/0); total (73/59)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

CGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTT  >  NC_000913/281045‑281536

cGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGCGTGCCCCACCCGAATAc                                                                                                                                                                                                                                                   >  2:19272/1‑251 (MQ=255)

    ggCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACAGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGATGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATAccc                                                                                                                                                                                                                                                 >  1:404343/1‑249 (MQ=255)

       gTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGCATACCCAGgcg                                                                                                                                                                                                                                            >  1:308916/1‑251 (MQ=255)

        ttGTGTTGCCGCTGTACTGCCGCGCTGGCGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGCTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGCGTGCCCCACCCGAATACCCAGgcgc                                                                                                                                                                                                                                           >  2:37074/1‑251 (MQ=255)

               gCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATCGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATAAGcc                                                                                                                                                                                                                                    >  1:77540/1‑251 (MQ=255)

                    tGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGAGAAAACGCTCCAGCTTCCCCTACGTCTGCGGAGGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGc                                                                                                                                                                                                                               >  2:408318/1‑251 (MQ=255)

                          cccGCGCTGGCGGCTGATCCCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAgc                                                                                                                                                                                                                         <  1:63916/250‑1 (MQ=255)

                           cggctcggccgggtgaccccGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAgcg                                                                                                                                                                                                                        <  2:165964/233‑1 (MQ=255)

                                 tGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACCGCTCCAGCGCCgacc                                                                                                                                                                                                                  >  2:453956/1‑248 (MQ=255)

                                 tGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTcc                                                                                                                                                                                                                  >  2:554243/1‑251 (MQ=255)

                                         gatcgcctgAGCCGGGTCCCGCCATTTCCATGGCCTCGTGCGTGCTTACAAGGTTATAGACCGGCCGCTCGTGGTCGAAGGCTCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCAGTGAACACGCTCCAGCTTCCCCTGCGTCGGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATCCCCAGGCGCATCAGCCTCATCTCCAGCGCCGTCCAGGTGcc                                                                                                                                                                                                           <  2:581108/242‑1 (MQ=255)

                                           ccccacttgcgggttgccGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATTCCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCggt                                                                                                                                                                                                        <  2:485509/235‑1 (MQ=255)

                                                 gcgccgggTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTc                                                                                                                                                                                                  <  1:216670/245‑1 (MQ=255)

                                                 gcccccgagaCCGCCATAGCCAGCGCCTCGTGCGGTCGTTCAAGGGTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCATTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACGTCCGCCTTCAGGCTGCGGGGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCATGTGCCTGTGGTGTc                                                                                                                                                                                                  <  2:308320/242‑1 (MQ=255)

                                                         tACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACg                                                                                                                                                                                          >  1:43994/1‑251 (MQ=255)

                                                                 tatCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGATGGCGCGCTGCAGTTCACCGCTGTCTGCGCACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGCTAGGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCTGGTGACGCTGGTGTCGCACCACGGTTAGCCg                                                                                                                                                                                  >  2:220179/1‑251 (MQ=255)

                                                                   tCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGtt                                                                                                                                                                                >  1:406760/1‑251 (MQ=255)

                                                                    ccAGCGCCGCGTGCGGGCGTTCAAGGTTATAGACCGGCCGCCAGTGGGCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGGCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTa                                                                                                                                                                               <  2:120870/251‑1 (MQ=255)

                                                                     cAGCGCCTCGAGCGGGCGTTCACGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTAt                                                                                                                                                                              >  2:385827/1‑251 (MQ=255)

                                                                             cGTGCGGGCTTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTc                                                                                                                                                                      <  2:485357/251‑1 (MQ=255)

                                                                             cGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTc                                                                                                                                                                      >  1:243647/1‑251 (MQ=255)

                                                                              gTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTc                                                                                                                                                                      >  1:510520/1‑250 (MQ=255)

                                                                              gTGCGGGCGTTCAAGGTTATACACCGTCCGCCAGTGGTCGAACGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGAGAACACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCCTCAGCCACAGCTCCAGCGCCGTCCAGGCGCCGGTGGTGTCGCCCCACGGTGAGCCGTAATCCATGGTc                                                                                                                                                                      >  2:535192/1‑250 (MQ=255)

                                                                                gCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAt                                                                                                                                                                    <  1:124293/250‑1 (MQ=255)

                                                                                 cGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtcc                                                                                                                                                                  <  1:398741/251‑1 (MQ=255)

                                                                                  gggCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtccg                                                                                                                                                                 >  1:5912/1‑251 (MQ=255)

                                                                                   ggCGTTCACGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCAACGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGGGAGCCGTTATCCATGGTCAtccgg                                                                                                                                                                >  2:110507/1‑251 (MQ=255)

                                                                                   ggCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGTTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtccgg                                                                                                                                                                <  2:308916/251‑1 (MQ=255)

                                                                                        tCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtccggtccg                                                                                                                                                            <  2:362289/250‑1 (MQ=255)

                                                                                         cAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGc                                                                                                                                                          >  1:216947/1‑251 (MQ=255)

                                                                                           aGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCAGCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAg                                                                                                                                                        <  2:427493/251‑1 (MQ=255)

                                                                                             gTTATAGCCCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGc                                                                                                                                                      <  1:163607/251‑1 (MQ=255)

                                                                                               tataGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGACCCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGCAAACGCTCCCGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCg                                                                                                                                                    >  2:185982/1‑251 (MQ=255)

                                                                                               tataGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCg                                                                                                                                                    >  1:542173/1‑251 (MQ=255)

                                                                                                ataGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGt                                                                                                                                                   >  2:161744/1‑251 (MQ=255)

                                                                                                         ccGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCTCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCa                                                                                                                                           <  2:470713/250‑1 (MQ=255)

                                                                                                         ccGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGTTCATCCGGTCCGGCAGGCCGTAACGCTCa                                                                                                                                           <  1:540284/250‑1 (MQ=255)

                                                                                                         ccGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCCGGCCGTAACGCTCa                                                                                                                                           >  2:212845/1‑250 (MQ=255)

                                                                                                           gCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAac                                                                                                                                        >  2:467638/1‑251 (MQ=255)

                                                                                                                tGGTCGCAGGCGCGCTGCCGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTACGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCAAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGAGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCt                                                                                                                                   >  2:378686/1‑251 (MQ=255)

                                                                                                                     gAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCCGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCGTGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGAcc                                                                                                                               >  1:70695/1‑250 (MQ=255)

                                                                                                                      aaGGCGAGCTGCAGATCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCCTGGTCATCCGGTCCGGCAGGCCGCAACGCTCAACCACGCTGAcc                                                                                                                               >  2:106106/1‑249 (MQ=255)

                                                                                                                             gcTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgctgctgc                                                                                                                      >  1:482108/1‑251 (MQ=255)

                                                                                                                             gcTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgctgctgc                                                                                                                      >  1:408976/1‑251 (MQ=255)

                                                                                                                                  aGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTCTCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAACCACGCTGCCCAGCTGCTGCTGCAc                                                                                                                 >  2:264109/1‑251 (MQ=255)

                                                                                                                                     tCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGt                                                                                                              >  2:166120/1‑251 (MQ=255)

                                                                                                                                        ccGCTGTCTGCGAACCATTCGCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTTCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATGCCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTTTCGCCCCACGGTGAGCCGTTAGCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCGGACCAgccgcgtcggtccggtctc                                                                                                           <  2:299513/251‑18 (MQ=255)

                                                                                                                                            tGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCgcgc                                                                                                       >  2:445388/1‑251 (MQ=255)

                                                                                                                                                 gCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCg                                                                                                     <  2:110187/248‑1 (MQ=255)

                                                                                                                                                     aCCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtcc                                                                                                                                                                  >  1:10430/1‑183 (MQ=255)

                                                                                                                                                     aCCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtcc                                                                                                                                                                  <  2:10430/183‑1 (MQ=255)

                                                                                                                                                       cATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGt                                                                                            >  1:302317/1‑251 (MQ=255)

                                                                                                                                                        aTTTTCCCTGCAGCACTTCCGCCTTCTGGCTGCGGTGAAAACGCTCCAGCTTACCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCCCGGAGAGCCGTTCTCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGAGCCGTTCAtacgt                                                                                            >  2:111306/1‑246 (MQ=255)

                                                                                                                                                              ccTGCAGCCCTGCCGCCTGCAGGCGGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCAGCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTgc                                                                                     <  2:323233/251‑1 (MQ=255)

                                                                                                                                                              ccTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCGCGCGCCGTTCATCGGTACAGTgc                                                                                     <  2:216947/251‑1 (MQ=255)

                                                                                                                                                               cTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTgcg                                                                                    >  1:453330/1‑251 (MQ=255)

                                                                                                                                                                 gcagcaCTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTgcg                                                                                    <  2:440856/249‑1 (MQ=255)

                                                                                                                                                                   agcaCTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGAGcc                                                                                  >  2:450267/1‑249 (MQ=255)

                                                                                                                                                                    gcccTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCCCCCGAATCCCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAgg                                                                               <  2:373441/248‑1 (MQ=255)

                                                                                                                                                                     caCTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGCATACCCAGGCGAATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGc                                                                              >  1:558460/1‑251 (MQ=255)

                                                                                                                                                                         tCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGcaca                                                                           <  2:382113/250‑1 (MQ=255)

                                                                                                                                                                         tCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACACAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCCGGCCGTAACGCTCAAACACGCTGACCCGCTGCTGCTGCACGGACTCGCGCCGTTCATCGGTAACGTGCGCCAGGcaccg                                                                          >  2:529754/1‑249 (MQ=255)

                                                                                                                                                                           cGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAggg                                                                        <  1:445388/251‑1 (MQ=255)

                                                                                                                                                                                tCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGaaa                                                                     <  1:478736/249‑1 (MQ=255)

                                                                                                                                                                                 cAGGCTGCGGTGAAACCGCCCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGGGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACATGGAAAAAc                                                                  <  2:409131/251‑1 (MQ=255)

                                                                                                                                                                                 cAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCCCGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAAc                                                                  >  2:479059/1‑251 (MQ=255)

                                                                                                                                                                                 cAGGCTCCGGTGACGACGCTCCAGCTTCGCCTGCGTCTGCGGCTGCTAAGGCCGGGAGTGCCCCACCCCAATACCCAGGCGAATCAGCCACACCTCAAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGATCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGATGACCAGCTGCTGCTGCACGGTCTCGCGCCGATCATCGGTCCACTGCGCCACGCCCCGGGaaacac                                                                  >  2:268467/1‑248 (MQ=255)

                                                                                                                                                                                      tGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACggg                                                               >  2:123027/1‑249 (MQ=255)

                                                                                                                                                                                       gCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGa                                                              <  1:385827/249‑1 (MQ=255)

                                                                                                                                                                                        cGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGCTGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTg                                                           >  2:586409/1‑251 (MQ=255)

                                                                                                                                                                                          gTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCGCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTAGCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGgt                                                         <  1:110507/251‑1 (MQ=255)

                                                                                                                                                                                              aaaCGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGGCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGgtcgtc                                                     <  2:522703/251‑1 (MQ=255)

                                                                                                                                                                                                aCGCTCCAGCTTCCCCTGCGTCTGCGGATGAGAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGGCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCca                                                   <  1:37074/251‑1 (MQ=255)

                                                                                                                                                                                                 cGCTCCAGCTTCCCCTGCGTCTGCGGATGATACGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCAACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCCCGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCCCAGGGAAAAACGGGAGTGgtcgccc                                                    >  2:518623/1‑246 (MQ=255)

                                                                                                                                                                                                 cGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCCTGGTCATCCGGTCCGGCAGGCCGTACCGCCCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGCCGTcc                                                    >  2:520161/1‑249 (MQ=255)

                                                                                                                                                                                                   cTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCCGGCACCGGGAAAAACGGGAGGGGTCGTCcagc                                                 >  2:346118/1‑250 (MQ=255)

                                                                                                                                                                                                      cAGCGTCCCCTGCGGCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCGGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAggg                                             <  2:66500/251‑1 (MQ=255)

                                                                                                                                                                                                           tCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGa                                          <  1:19272/249‑1 (MQ=255)

                                                                                                                                                                                                            ccccTGCGGCTGCGGACGACAAGGTCGGGAGGGCCCCAACCGAAGCCCCAGGCGCATCAGCCACAGCTCCAGGGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGGGTGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACCCGCTGACCAGCGGCTGCTGCACGGTCTCGCGCCGTTCAACGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCATCAGGGTGAg                                         <  2:337981/249‑1 (MQ=255)

                                                                                                                                                                                                                 gCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATgg                                  <  1:470413/251‑1 (MQ=255)

                                                                                                                                                                                                                 gCGTCTGCGGATGATAAGGACGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCCCAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTTAGCCGTTATCCATGGTCAGCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCGGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGAt                                    <  2:430753/249‑1 (MQ=255)

                                                                                                                                                                                                                  cGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGc                                 <  1:110079/251‑1 (MQ=255)

                                                                                                                                                                                                                   gTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCATAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGc                                 >  2:194425/1‑250 (MQ=255)

                                                                                                                                                                                                                        cGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTc                           <  1:37621/251‑1 (MQ=255)

                                                                                                                                                                                                                         ggtggatAAGGCCGGGAGTTCCCCACCCGAATACCCAGGCGACTCAGACACAGCGCCAGCGCCGTACAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATACGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGGGCCAGGCACAGGGAAAAACGGGAGTGGACGTCTAGCAGGGTGAGCGGATGGCAGCGTcc                          <  2:167406/247‑1 (MQ=255)

                                                                                                                                                                                                                          gatgatAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCa                         <  1:260536/251‑1 (MQ=255)

                                                                                                                                                                                                                             gatAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCAcc                       <  2:571281/250‑1 (MQ=255)

                                                                                                                                                                                                                             gatAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCGCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGGCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCAcc                       <  2:285195/250‑1 (MQ=255)

                                                                                                                                                                                                                                aaGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAACCGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCCCCGcc                    >  2:459537/1‑250 (MQ=255)

                                                                                                                                                                                                                                 aGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCaa                  <  1:479059/251‑1 (MQ=255)

                                                                                                                                                                                                                                  gggcgggaggtccccACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCGGTCCAGGGGCCGGTGGTGCCGCCCCACGGTGAGACGTTACCCTTGGTCATCCGTTCCGGCAGGCCGTAACGCTCAAACACTCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCTTCCATCAGGGTGAGCGTGTGGCAGCGTCCACCGCCaaa                 <  2:396354/240‑1 (MQ=255)

                                                                                                                                                                                                                                  ggCCGGGAGTGCCCCGCCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGTTGGTGTCGCCCCACGGTGAGCCGTTATCCATGTTCAGCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTCCTGCACGGTCTCGCGCGTTTCATCGGTACATTGCGCCAGGCACAGGAAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCTTCCACCGCCaaa                 <  1:494076/251‑1 (MQ=255)

                                                                                                                                                                                                                                  ggCCGGGAGTGCCCCACCCGAATACCCGGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCaaa                 <  2:510520/251‑1 (MQ=255)

                                                                                                                                                                                                                                  ggCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCaa                  >  1:551793/1‑250 (MQ=255)

                                                                                                                                                                                                                                   gCCGGGAGTGCCCCCCCCGAATACCCGGGCTCATCAGCCACTGCGCCAGCGCCGTCCTGGTTCCGGTGGTGTCGCCCCTCGGTGAGACGGTATCCATGGTCATCCGGTCCGGCAGGCCGTATCGCTCAAACACTCTGACCAGCTGCTTCTGCTCGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGGGGTCGTCCAGCAGGGTTAGCGTATGTCAGCGTCCACCGCCaaaa                <  2:405824/251‑1 (MQ=255)

                                                                                                                                                                                                                                        gAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGaa            >  1:181901/1‑250 (MQ=255)

                                                                                                                                                                                                                                            gCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTgg       <  2:181901/251‑1 (MQ=255)

                                                                                                                                                                                                                                              cccACCCGAATACCCAGGCGCATCAGCCAAAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCACAAGGAAAGTGGcc     >  2:584256/1‑251 (MQ=255)

                                                                                                                                                                                                                                                 aCCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCtt  >  1:355118/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  cccGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTGACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGc                              >  1:426636/1‑222 (MQ=255)

CGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTT  >  NC_000913/281045‑281536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`