Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,080,405	C→T	G63S (GGT→AGT)	yeeA ←	putative transporter YeeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,080,405	0	C	T	97.1%	102.7 / NA	34	G63S (GGT→AGT)	yeeA	putative transporter YeeA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/19);  minor base G (0/1);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAACTCATC‑TGTCGGCCAGAAGGCGCG  >  NC_000913/2080164‑2080651                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        cATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACg                                                                                                                                                                                                                                                 >  1:449448/1‑250 (MQ=255)    aaCGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCTTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGAt                                                                                                                                                                                                                                             <  1:197537/251‑1 (MQ=255)         cGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATACGCCCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGcc                                                                                                                                                                                                                                        >  2:484114/1‑251 (MQ=255)                        tGTCGGGGAACCCACAACAGTTGCCAGCGTCACACCAATCAATAAACATAGGTACGGTTTCTTGCCCAGCGCAAGCCAACCGCAAAGGAACATGGCCGCCGCGCAACAGACTAACATCAGCGGTAGCGAGATTAACTCCAGTTGTAGAGCGAAAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCGCACAGGCGCGAGTGACAACGTTACTCCAGAACGAAATTGGCACAATAATCaccacct                                                                                                                                                                                                                         >  1:295278/1‑250 (MQ=255)                                 aCCCACAACAATTGCCCGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGACCCCATAATCACCACCATGGTgccc                                                                                                                                                                                                                 >  1:408257/1‑247 (MQ=255)                                  cccACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGTTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAAGCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAg                                                                                                                                                                                                               <  2:306853/251‑1 (MQ=255)                                  cccACAACAATTGCCACCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCTAGCGACAGCCAACCGAAAAGGACCATGGCCGCCGCGCACCACACTAACATCATCGGTAACGAGATTAACCCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGATGGACAACGTTACTCCAGAACGAGCTTGGCCCCATAATCACCACCATGGTGACCAg                                                                                                                                                                                                               <  1:132099/251‑1 (MQ=255)                                  cccACAACAAGTGCCAGCGTCCCCACAAGCAATAAACCTTGACACGGTTTCTGGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAATATCAGCGGTAACGAGATTAACTCCTGTGGCAGAGCGATAAGACCTAAAATCGATCCCAAGGCCGTACCGCCAATACGCTCAAATGCGCGAGGGACATCGTTACGCCAGAGCTAGATTGGCCCCATAAGCTCCGCCATggctaccag                                                                                                                                                                                                               <  2:408283/251‑8 (MQ=255)                                               ccAGCGTCAGACCAATCAATAAACCTTGATACGGTTTCTAGCCCAGCGCCAGCCCACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTACCGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGCGGGACCACGTTACTCCAGAACGAGATTGGCCCCATACTCACCACCATGGTGACCAGCGGCCAggagcct                                                                                                                                                                                                  >  2:387165/1‑246 (MQ=255)                                                            aatcaatAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTa                                                                                                                                                                                      >  1:168466/1‑250 (MQ=255)                                                             accaatAAACCTTGCTACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACAAGACTAACATCAGCGGTAACGGGATTAACCCCAGTTGCAGAGCGATAAGACCCAAAATCGAACCCAACACCTTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTaaa                                                                                                                                                                                    <  1:567191/249‑1 (MQ=255)                                                                aatAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAg                                                                                                                                                                                 <  1:92449/251‑1 (MQ=255)                                                                          gATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCAAAAACCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAAt                                                                                                                                                                        <  1:14137/250‑1 (MQ=255)                                                                          gATACGGTTTCTTGCCCAGCCCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCACCCTAACATCAGCGTTAACGAGATTAACTCAAGTTCCAGAGCGAAAAGACCTAAAATCGAACCCAACGCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAAt                                                                                                                                                                        <  2:165659/250‑1 (MQ=255)                                                                               ggTTTATTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGt                                                                                                                                                                                                      >  1:485355/1‑215 (MQ=255)                                                                                      tGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTg                                                                                                                                                              <  1:363875/248‑1 (MQ=255)                                                                                      tGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGc                                                                                                                                                           >  2:209367/1‑251 (MQ=255)                                                                                                cagccaACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAACCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCa                                                                                                                                                 <  2:430553/251‑1 (MQ=255)                                                                                                            aaGGAACATGGCCGCCGCGAACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGGGAGCAGGAATGCCAGCGCGACCCg                                                                                                                                       >  2:60990/1‑249 (MQ=255)                                                                                                                  cATGGCCGCCGCGCACCAGACTAACACCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCAt                                                                                                                               <  1:563472/251‑1 (MQ=255)                                                                                                                           cgcgCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGc                                                                                                                      >  2:360960/1‑251 (MQ=255)                                                                                                                            gcgcACCAAACTAACATCAGCGTTAGCGAGATTAACCCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCg                                                                                                                     <  2:208363/251‑1 (MQ=255)                                                                                                                                 ccAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAAt                                                                                                                <  1:60990/251‑1 (MQ=255)                                                                                                                                   aGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATgg                                                                                                              <  1:245400/251‑1 (MQ=255)                                                                                                                                          cATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGtata                                                                                                       <  1:433833/251‑1 (MQ=255)                                                                                                                                                  gTAACGAGATTAACTCCAGTTGCAGAGCGATAAGCCCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATg                                                                                                                                                                                                                       <  2:371451/131‑1 (MQ=255)                                                                                                                                                  gTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATg                                                                                                                                                                                                                       >  1:371451/1‑131 (MQ=255)                                                                                                                                                                 ccAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTa                                                                                  <  1:366254/249‑1 (MQ=255)                                                                                                                                                                                               aaCCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGt                                                  <  1:360960/251‑1 (MQ=255)                                                                                                                                                                                                            aCCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGt                                                  >  1:565961/1‑238 (MQ=255)                                                                                                                                                                                                            aCCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGt                                                  <  2:565961/238‑1 (MQ=255)                                                                                                                                                                                                                       gcgcAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAAc                          <  2:449448/248‑1 (MQ=255)                                                                                                                                                                                                                                   gAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGGGTACCATGCACAATGCGGTAATGGCGGTATAACCGGATTTCAAACGGGCTTTATGACTTATCGGCACGCACGCAGAACCTCCGTAATGAATTTAAAAGCGCTCATAACTCCTC‑TGTcagc             >  2:478444/1‑248 (MQ=255)                                                                                                                                                                                                                                              ttACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACACGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGCCTTATCGGCACGCACGCAGAAAATCCGGAATGAGAATAAAAGCGCTCATAACGCATCATG‑CGGCCCGAAGgcgcg  >  2:165204/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        CATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAACTCATC‑TGTCGGCCAGAAGGCGCG  >  NC_000913/2080164‑2080651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑