Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	276,899	G→A	G62E (GGG→GAG)	mmuM →	homocysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	276,899	0	G	A	100.0%	207.2 / NA	64	G62E (GGG→GAG)	mmuM	homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/28);  total (36/28)

GTTGTGCTATGGTGCTTATTTCCTTACTCAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGG‑ATCCG‑TCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTT  >  NC_000913/276656‑277143                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       gTTGTGCTATGGTGCTTATTTCCTTACTCAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGAAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCa                                                                                                                                                                                                                                                  >  1:496352/1‑250 (MQ=255)         aTGGTGCTTATTTCCTTACTCAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACATGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTTTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGa                                                                                                                                                                                                                                          <  1:569497/250‑1 (MQ=255)                  aTTTCCTTACTCAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCa                                                                                                                                                                                                                                 <  1:420346/250‑1 (MQ=255)                     tCCTTACTCAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCt                                                                                                                                                                                                                              >  2:40281/1‑250 (MQ=255)                      ccTTACTCAACACCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCACGATGGCGACGGAGCTGGAAGCGCGAGGGAGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAACTTATCCGCGAAGGGCATCTTGATTACTACCGGGCGGAGGCGCAATGTGCGCTCACTGCCAGCTAt                                                                                                                                                                                                                            >  2:386195/1‑251 (MQ=255)                         tACTCAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGAGCACTGCCAGCTATCa                                                                                                                                                                                                                          <  1:445815/250‑1 (MQ=255)                             cAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCg                                                                                                                                                                                                                      <  2:84661/250‑1 (MQ=255)                              aaCCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGAc                                                                                                                                                                                                                    >  2:126653/1‑251 (MQ=255)                                    gAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGCCGCcggc                                                                                                                                                                                                              >  2:217716/1‑251 (MQ=255)                                          cAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCtt                                                                                                                                                                                                        >  1:315295/1‑251 (MQ=255)                                           aaaaCAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTTCCAGCTATCAGGCGACGCCGGCGGGCTTc                                                                                                                                                                                                       <  1:488334/251‑1 (MQ=255)                                           aaaaCAGGAGACAGAACATGTCGCAGAATAATCCGTTAAGCGCTCTTCTTGATAAACAGGATAACCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTc                                                                                                                                                                                                       <  2:432836/251‑1 (MQ=255)                                              aCAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTcgcc                                                                                                                                                                                                    >  1:262043/1‑251 (MQ=255)                                                         aaCATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAGACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCt                                                                                                                                                                                         <  2:487165/251‑1 (MQ=255)                                                              gTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGCGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGGCTgggtg                                                                                                                                                                                    >  2:66451/1‑248 (MQ=255)                                                                      ataatCCGTTACGCGCTCTTCTTGATAACCAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGGCTGGATGAAgcgc                                                                                                                                                                              >  2:264994/1‑249 (MQ=255)                                                                       taatCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGt                                                                                                                                                                           >  1:345595/1‑251 (MQ=255)                                                                         atCCGTTCCGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCg                                                                                                                                                                         <  2:328358/251‑1 (MQ=255)                                                                                aCGCGCTCTTCTTGCTAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGCCGCCGGCGGGCGTCGCCGCGCGCGGTCTGGATGAGGCGCAGTCTAAAgagc                                                                                                                                                                  >  2:438188/1‑248 (MQ=255)                                                                                aCGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGACAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGCGGACTGGATGCAGCGCAGTCGaacacgc                                                                                                                                                                  >  1:415197/1‑246 (MQ=255)                                                                                  gcgcTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTAATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGGCGCGCGGGGGCTGGATGAAGCGCAGTAGAAAGCGCTg                                                                                                                                                                >  2:46602/1‑251 (MQ=255)                                                                                   cgcTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCCCCGCGCGGGGGCTGGATGAAGCGCAGGCGAAAGCGCTg                                                                                                                                                                >  2:280646/1‑250 (MQ=255)                                                                                     ctctTCTTGATAAACAGAATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCCTCGCCGCGCGCGGGCTGGCTGAAGCGCAGACGAAAGCGCTGAtt                                                                                                                                                             >  2:49046/1‑251 (MQ=255)                                                                                       cttcttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGAtt                                                                                                                                                             <  1:79250/249‑1 (MQ=255)                                                                                        ttcttGATAAAAAGGATCTCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGGGGGCTTGATGAAGCGCCGGCGAAAGCGCTGATTGGc                                                                                                                                                          >  2:496621/1‑251 (MQ=255)                                                                                          cttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGCGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTTGCCGCGCGGGGTCTGGGAGTCACGCAGTCGAAAGCGCGGATTGGcc                                                                                                                                                         >  2:314413/1‑249 (MQ=255)                                                                                           ttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGAGCGAGGGTGTACCTTAGCCGACAGCCTGTGGTCAGCCAACGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTAACGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGGGGTCTGGGTGAAGCGCCGTCGAACGCGCTGATTGGCaa                                                                                                                                                        >  2:526542/1‑250 (MQ=255)                                                                                                aaacatgagttCCTGCTGCTGGAGTGCGCGAGTGCTACGGAGCTGGAAGCGCGAGGGTGTAACGTAGCCTACAGCCTGTGGTCATCCAAAGTGCTGGTAGAAAACCCGGAGCTTAGCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGAAATGCGCGATCACTGCCAGCTATCAGGCGACGCCTGCGGGCTGCGCCGCGCTAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGt                                                                                                                                                  <  2:518823/241‑1 (MQ=255)                                                                                                      gATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGGCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCt                                                                                                                                            >  1:28284/1‑251 (MQ=255)                                                                                                      gATATCCTGCTGCTGGATGGCGCGATGGCGAAGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGCTTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGACTTCGCCGCGCGGGGTCTGGATGAAGCGCAGTCGAAAGCGCGGCTTGGCAAAAGCggtgaacg                                                                                                                                            >  1:497738/1‑244 (MQ=255)                                                                                                              gctgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGTAAACCCGGTGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCATCGCCGCGCGAGGTCTGGGTGACGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGt                                                                                                                                     >  2:392061/1‑250 (MQ=255)                                                                                                                tgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGCGTAACTTAGCCGACAGCCTGTGGTCAGCCAACGTGCTGGTAGAAACCCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCCGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCCGTCCAAAGCGCTGAGTGGGCAAAGCGGGGAGcggggccggaa                                                                                                                                   >  2:252904/1‑240 (MQ=255)                                                                                                                 gctgGATGGCGCGATGGCTACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTaaa                                                                                                                                  <  2:104525/250‑1 (MQ=255)                                                                                                                 gctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTa                                                                                                                                    <  1:155790/248‑1 (MQ=255)                                                                                                                  ctgGATGGCGCGATGGCTACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGGCTGGATGAAGCTCAGTCGAAAGCGCTGCTTTGCCAAAGCGGGGGGCTGGCGCGTAAAGc                                                                                                                                >  2:118710/1‑251 (MQ=255)                                                                                                                    ggATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACGTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAACCCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCATTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGccc                                                                                                                              <  1:526542/251‑1 (MQ=255)                                                                                                                           gcgATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGGGCTGGCGCGTAAAGCCCGTGAAGc                                                                                                                       >  1:247140/1‑251 (MQ=255)                                                                                                                               tGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTAt                                                                                                                   >  1:368482/1‑251 (MQ=255)                                                                                                                                ggCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTAt                                                                                                                   <  1:8863/250‑1 (MQ=255)                                                                                                                                   gACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAATCCCGGAGCTTAGCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATGGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTgg                                                                                                               <  1:363710/251‑1 (MQ=255)                                                                                                                                   gACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTgg                                                                                                               <  1:288704/251‑1 (MQ=255)                                                                                                                                     cGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCa                                                                                                             <  1:40281/251‑1 (MQ=255)                                                                                                                                      ggAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCag                                                                                                            <  2:181206/251‑1 (MQ=255)                                                                                                                                             gAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGACAGCGCTGATTTGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTTAAGCGTATCTGGCAGAGAAccc                                                                                                     >  1:49228/1‑251 (MQ=255)                                                                                                                                             gAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGTCAGAGAAccc                                                                                                     <  2:543331/251‑1 (MQ=255)                                                                                                                                                gcgcGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGAGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGGAGCGTATCTGGCCGAGAACCCGc                                                                                                   >  1:514370/1‑250 (MQ=255)                                                                                                                                                gcgcGAGGGTGTAACTGAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTTCATCTTGATTGCTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGAGGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTTGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCa                                                                                                  <  1:520832/251‑1 (MQ=255)                                                                                                                                                  gcgAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGGGAAGCGTATCTGGCAGAGAACCCGCAgg                                                                                                >  1:376716/1‑251 (MQ=255)                                                                                                                                                                   ccGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCtggtg                                                                               <  1:60963/251‑1 (MQ=255)                                                                                                                                                                    cGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCtggggg                                                                              >  1:230271/1‑248 (MQ=255)                                                                                                                                                                     gACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGGATCTGGCAGAGAACCCGCAGGCGGGGACGCTTTtgggggg                                                                             >  1:497229/1‑247 (MQ=255)                                                                                                                                                                        aGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGGATCTGGCAGAGAACCCGCAGGCGGGGACGCCTCTTGTGGCggg                                                                          >  1:415556/1‑251 (MQ=255)                                                                                                                                                                          ccTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTAGCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGG‑At                                                                       <  2:497229/251‑1 (MQ=255)                                                                                                                                                                                gTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCTCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCTCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGG‑ATCCG‑TCg                                                                <  1:280267/251‑1 (MQ=255)                                                                                                                                                                                       aaaGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGGAGCGTATCTGGCAGAGAACCCGCAGGCGGGGACGCCTCtggggggggggttccgccgggcttt                                                           >  2:343457/1‑229 (MQ=255)                                                                                                                                                                                         aGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGACGCGTATCTGGCAGAGAACCCGCAGGCGGGAAACCCTCTGGGGGCGGG‑GTCCGGTCGG‑CCTTAcc                                                       >  2:273473/1‑250 (MQ=255)                                                                                                                                                                                         aGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGAATCTTGATTACTACCGGGCGGAGGCTCCATGCTCGATCACCGCCAGCTATCAGGCTACGCCGGCGGGCTTCGCCGCGAGTGGTCTGGGTGAAGCGCAGTCGAAAGAGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCGCGTGAAGCGGATCTGGCAGAGAACCCGCAGGCGGGAACGGTGCCGGTTTCGGG‑ATCCG‑TCTGGCCTTACg                                                       <  2:100365/251‑1 (MQ=255)                                                                                                                                                                                                           ggAGCTTCTCCGCGAAGTGCCTCTTTATTACTCCCGTGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCTGGCGGGAACGCTTCTGGTGGCGGG‑ATCCG‑TCGGGCCTTACGTCGCGTATCTGGCGGATg                                     <  2:496352/251‑1 (MQ=255)                                                                                                                                                                                                                    ccGCGAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGG‑ATCCG‑TCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGaa                             <  2:157847/250‑1 (MQ=255)                                                                                                                                                                                                                       cgAAGTGCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGGACGCTTCTGGGGGCGGG‑ATCCGGCCGGGCCT‑ACGGGGCGGATCTGGCGGGTgggtccggatacc                         >  1:5772/1‑240 (MQ=255)                                                                                                                                                                                                                          aGTGCATCTTGATTTCTACCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGG‑ATCCG‑TCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTg                      <  1:479285/251‑1 (MQ=255)                                                                                                                                                                                                                             gCATCTTGATTACTGCCGGGCGGAGGCGCAGTGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCGTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGG‑ATCCG‑GCGGGCCTTACGGCGCGTATCTGGCGTTTGGCTCTGAATACCGTGGCg                   <  2:48229/251‑1 (MQ=255)                                                                                                                                                                                                                             gCATCTTGATTACTACCGGGCGGAGGCGCAATGCGCGATCACTGACAGCTATCAGGCGACGCCGGCGGGCTACGCCGCGCGGGGTCTGGATGAAGCGCAGTCGAAAGCGCTGGTTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGAGAAGCGTATCTCGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGGATA‑G‑ACGGGCCTTACGGCGCGGATTTGGGGGGTGGCACTGAATAcccggggg                   >  1:571384/1‑245 (MQ=255)                                                                                                                                                                                                                                           acCGGGCGGAGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCCTCTGGTGGCGGG‑ATCCG‑TCGGGCCCTACGGCGCGTATCTGGCGGATGGCTCTGTATACCGTGGCGATTATCATTTTAGc     >  1:531469/1‑251 (MQ=255)                                                                                                                                                                                                                                              gggcggaGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGG‑ATCCG‑TCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGtt  <  2:262043/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GTTGTGCTATGGTGCTTATTTCCTTACTCAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGG‑ATCCG‑TCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTT  >  NC_000913/276656‑277143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑