Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,839	T→C	L12P (CTC→CCC)	pabB →	aminodeoxychorismate synthase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,839	0	T	C	100.0%	146.2 / NA	44	L12P (CTC→CCC)	pabB	aminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/20);  total (24/20)

AGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACATTCG  >  NC_000913/1894596‑1895082                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    aGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGt                                                                                                                                                                                                                                              <  1:249009/251‑1 (MQ=255)   tggttaccACGCAGATATTCTGCCACCAGGGCGAGTGCATGGCCGCAGCTCATACCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGATCATGGGTGAGTGAAGGGAAACCTGCAAACAGTGGTCACTCCTGCTAAATTGTTGGCGCTACTTATTTCATGCTACCCGGCACATAGCCAGTAGAGTGAGGACTGATGAAGCCGTTATCTCCCGCTGTGATTACTTTACCCTGCCGTc                                                                                                                                                                                                                                             >  2:269678/8‑250 (MQ=255)       tGGCACGCAGCTCGTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCGGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGa                                                                                                                                                                                                                                         <  2:516234/250‑1 (MQ=255)          cACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATGTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGAcgcc                                                                                                                                                                                                                                     <  1:254999/251‑1 (MQ=255)                 ctctTCTGCCACCGGCGAACTTCCCTGGCGGCTGCTCCTCCCTCGTGCCATCAGTTCCTGGATCCGAACGAAAGCTTTTCGCTACTGTTCATGGGAGAGTGCAGGTAAACCTGCAAACATTGTTACCTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACCTAGCCAGCAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGCTTACTTTACCCTGGCGTCAGGACGCCGCTGAAt                                                                                                                                                                                                                              >  2:242150/1‑251 (MQ=255)                          caccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTCACTCCTGCTCAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGCGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTtct                                                                                                                                                                                                                     >  1:302092/1‑251 (MQ=255)                                cgcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCGAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTTATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCt                                                                                                                                                                                                               <  1:563916/251‑1 (MQ=255)                                   gATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTGTTTGCTGCTGGTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTTCCCGGCACATAGCCAGTGGAGTCAGGACTGATGAAGACGGTATCTCCCGCTGTGATTGCTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTaa                                                                                                                                                                                                            <  1:60960/251‑1 (MQ=255)                                                            tgtgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCtttt                                                                                                                                                                                   >  1:91205/1‑251 (MQ=255)                                                            tgtgCCATCAGTTCCTAGATCAGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCtttt                                                                                                                                                                                   >  2:108244/1‑251 (MQ=255)                                                               gCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTaca                                                                                                                                                                                <  1:178147/251‑1 (MQ=255)                                                                    cAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTcc                                                                                                                                                                            <  2:156522/250‑1 (MQ=255)                                                                            ggagccGCTCGACAGCTTTGTGCTGCTGTGCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCGGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCGTAAGCCACCTGCCGTGGGCGATGCTGTTACACTCCGGCTATGcc                                                                                                                                                                   <  2:501668/247‑1 (MQ=255)                                                                                ccGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCCCCTGCCGTGTGCGATGCTTTGACACCCCGGCTATGCCGat                                                                                                                                                                <  1:464293/250‑1 (MQ=255)                                                                                                 gctgctGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCttt                                                                                                                                              >  1:438952/1‑251 (MQ=255)                                                                                                   tgctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCAACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTg                                                                                                                                             >  2:518337/1‑250 (MQ=255)                                                                                                             gggTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTc                                                                                                                                  <  2:325702/251‑1 (MQ=255)                                                                                                              ggTGATTGAAGGTAAACCTGCAAAAATTGTTAACTCCTGCTAAATTGGTGGCGCTAATTATTACATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACGTTTCCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCTCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCGATGCCGATCATCCGTCTAGCCGCTTTGATAGTGTGGTCg                                                                                                                                 <  2:492024/251‑1 (MQ=255)                                                                                                                         gTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTGTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGAtt                                                                                                                      >  1:153193/1‑251 (MQ=255)                                                                                                                           aaaCCTGCCAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGCGTCAGGACTGCTGCAGACGTTATCTCCCGCTGTGCTTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTCTTTCTCCCGCTTAAGCCACCTGCCGTGGGCGCTGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTg                                                                                                                    >  2:369909/1‑251 (MQ=255)                                                                                                                               cTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACt                                                                                                                >  1:420056/1‑251 (MQ=255)                                                                                                                                    aaCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAAc                                                                                                           >  1:204235/1‑251 (MQ=255)                                                                                                                                                        aaTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCg                                                                                       <  1:518337/251‑1 (MQ=255)                                                                                                                                                         aTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCgt                                                                                      >  1:265844/1‑251 (MQ=255)                                                                                                                                                                   cTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAATCAGGACTGATGAAGTTGTTATCTCCCACTGAGATTACTTTACCCTGGCGCCAGGACTCCGCTGAATTTTATATCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATTCCGTTACTCCGTTTAGCCGCTTTAATATTATGTTCGCCAACCCGCTTTGCACTCTACACACTTTAGG‑AAAGAAAcccatttttagtgt                                                                            >  1:15519/1‑238 (MQ=255)                                                                                                                                                                     aaTTATTTCCTGCTCCCCGCCACATAGCCCGTAGAGACCGGCCTGCTGACGACCTTATCTACCGCTGTGATTACTTTACCCTGGCGTCCGGACGCCCCTGCATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGCGCCGCTTTGCACTTTAACCACt                                                                                                       >  2:353798/1‑222 (MQ=255)                                                                                                                                                                     aaTTATGTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGGTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTGATTTCTCCGGCTTAAGCCGCCTGCCGTGGGCGATGCTTTTACGCTCCGGCTATGCCGATCATCCTTATAGCCGCGTTGATATTGTGGGCGCCGAGCCGATTTACACTATAACCACt                                                                                                       <  1:353798/222‑1 (MQ=255)                                                                                                                                                                       ttattTCATGCTACCCGGCACATAGCCAGCAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTATACCCTCGCGTCAGGCCGCCGCTGACTTTTATTTCTCCAGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATACCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAACGAAACCGTTGTTAGTGAAAGCg                                                                        >  1:25817/1‑251 (MQ=255)                                                                                                                                                                        tattTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCg                                                                        <  2:423195/250‑1 (MQ=255)                                                                                                                                                                         attacATGCTCCCCGGCACATAGCCAGTAGAGTCAGGACTGAGGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGTACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCTTGGGCGATGCTTTTACACTCCGGCTATGCCGATCAGCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGa                                                                       <  1:369909/246‑1 (MQ=255)                                                                                                                                                                         attTCATGCTACCCGGCACATAGCCAGTAGCGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGa                                                                       >  2:76687/1‑250 (MQ=255)                                                                                                                                                                                gCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGc                                                                >  1:219371/1‑250 (MQ=255)                                                                                                                                                                                 cTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGcac                                                              >  2:196776/1‑251 (MQ=255)                                                                                                                                                                                         cacaTAGCCAGTAGAGTCAGGACTGATGACGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGAcc                                                       >  1:462033/1‑250 (MQ=255)                                                                                                                                                                                              aGCCAGTTGAGTCAGGCCTGATGAAGTCGTTACCTCCCGCTGTGATTACTTTCCCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGAACACtgat                                                 <  1:523508/251‑1 (MQ=255)                                                                                                                                                                                                ccAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACtgatg                                                >  2:275357/1‑250 (MQ=255)                                                                                                                                                                                                    tAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAg                                                                                                                                                     >  1:454274/1‑145 (MQ=255)                                                                                                                                                                                                    tAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAg                                                                                                                                                     <  2:454274/145‑1 (MQ=255)                                                                                                                                                                                                          cAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGAGATTGTGGTCGCCGAGCCGATTTGCACTTTAACCTCTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACa                                      <  1:252147/250‑1 (MQ=255)                                                                                                                                                                                                           aGGACTGCTGAAGCCGTTATCTCCCGCTGCGCTTACTTTACCCTGGCGAGAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCTCCGAGCCGATTTGCACTTTAACCACTCTCGGTAAAGAAACCGTTGTTAGAGAAAGCGAAAAACGCACAACGACCACTGATGCCCCGCTAccgg                                    >  2:45926/1‑248 (MQ=255)                                                                                                                                                                                                                    gaAGACGTTATCTCCCGCTGGGATTCCTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCcag                            <  2:91205/250‑1 (MQ=255)                                                                                                                                                                                                                                   cGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATcgcg            >  2:80124/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCgcgc           >  2:483433/1‑251 (MQ=255)                                                                                                                                                                                                                                       gtgATTTCTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACGCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATGGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAg         <  1:242150/250‑1 (MQ=255)                                                                                                                                                                                                                                             actttaccCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACATTCg  >  1:514611/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    AGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACATTCG  >  NC_000913/1894596‑1895082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑