Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,623	C→T	E63K (GAA→AAA)	dmlR ←	DNA‑binding transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,623	0	C	T	100.0%	231.3 / NA	67	E63K (GAA→AAA)	dmlR	DNA‑binding transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/39);  total (28/39)

GAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCA  >  NC_000913/1881378‑1881860                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              gagaTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTa                                                                                                                                                                                                                                           >  2:3693/1‑250 (MQ=255)     tCGACACATTCATTGAACAAATCGACCAGTCGGTCTTACACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGACGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATACGTAGCGCACATTCATAAATCCGCTCTCCTTCTTTGGTAATGGt                                                                                                                                                                                                                                      >  2:18294/1‑251 (MQ=255)       gACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTg                                                                                                                                                                                                                                     >  1:110204/1‑250 (MQ=255)        acacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGAc                                                                                                                                                                                                                                   >  1:448808/1‑251 (MQ=255)                        aaTCGACCAGTCGGTCTTCCACATCAACACGTGCTTCCAGTTGCGGATACGCCTTCGCCAGCGCTCATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAACTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGATCGTCCATCATCTGACCGCCATCCTGCAAACTCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTACTGGTGACGCGGCgggggtggcgg                                                                                                                                                                                                                   >  2:566100/1‑243 (MQ=255)                                    ggTCTTCCACATCAAAACGTAATTCAAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGAGGTGTAACAAcacc                                                                                                                                                                                                       >  2:555582/1‑251 (MQ=255)                                     gTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAAcacc                                                                                                                                                                                                       >  1:392573/1‑250 (MQ=255)                                           cacaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTa                                                                                                                                                                                                <  1:193611/251‑1 (MQ=255)                                              aTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCg                                                                                                                                                                                             >  1:161368/1‑251 (MQ=255)                                                cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGt                                                                                                                                                                                            <  1:344292/250‑1 (MQ=255)                                                 aaaaCGTAATGCCAGTTGCGGATTCGCCTTCGCCAGCGCTAATAACTCCGGAGCCACCACCTGTCGTCCAAAACCAAATCTTCTGATAATACGCAGCATCCCCTGCTGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCACAAATCCTCTCTCCTTCTTTGCTAATGTTGACTCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGttt                                                                                                                                                                                          <  1:330974/251‑1 (MQ=255)                                                   aaCGTAATTCCAGTCGCGGATACGCCTTCGCCAGCGCTAATAACTCCGGAGCCACCTCCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCGCCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATGCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGTGCTGTAACATCACCTCGTTTAGcttgtgc                                                                                                                                                                                        <  2:333525/251‑7 (MQ=255)                                                      gTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAAGCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTcc                                                                                                                                                                                     <  1:542619/251‑1 (MQ=255)                                                        aaTTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTcc                                                                                                                                                                                     >  1:432789/1‑249 (MQ=255)                                                         aTTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCACCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGc                                                                                                                                                                                  <  1:63989/251‑1 (MQ=255)                                                                  gCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGAt                                                                                                                                                                          <  1:10423/250‑1 (MQ=255)                                                                  gCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATg                                                                                                                                                                         <  1:174739/251‑1 (MQ=255)                                                                        aCGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCttt                                                                                                                                                                   >  2:157094/1‑250 (MQ=255)                                                                        aCGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTg                                                                                                                                                                   <  2:120369/251‑1 (MQ=255)                                                                         cGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCACAGCTGCTGATAATACGAAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGc                                                                                                                                                                  >  1:266654/1‑251 (MQ=255)                                                                          cccgtCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCAGCCCCTGCGGCTCCTGACGCACGTCAGAAAGTTCGTCCAGCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAAGCCGCTCTCCGTCTTTGGTGATGGTGACGCGGCGGGTGGTGCGAGGTAACAACACCACGTGTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCt                                                                                                                                                                 <  1:536718/247‑1 (MQ=255)                                                                                    gcgcTAATAACGCCGGAGCCACCACCTGTCGTCCAACACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGACCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGGGACGCGGCGGGTGGTGCGGTGTACCAACACCACGGTTAGCGTTAGCTCCAGAAAGGCGATGCGCTTGCTGACGAAAGcc                                                                                                                                                       >  2:422816/1‑251 (MQ=255)                                                                                                             ccgtcgtcCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCGTCGGCACCTGACGCGCGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTTCATCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACTCCCAGGTCTGCCGCCa                                                                                                                              <  2:102781/249‑1 (MQ=255)                                                                                                              tGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAg                                                                                                                                                                               >  1:151414/1‑201 (MQ=255)                                                                                                              tGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAg                                                                                                                                                                               <  2:151414/201‑1 (MQ=255)                                                                                                               gtcgtcCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCAcg                                                                                                                            <  1:314430/251‑1 (MQ=255)                                                                                                               gtcgtcCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCAcg                                                                                                                            <  2:473412/251‑1 (MQ=255)                                                                                                                  gtcCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCAcggcg                                                                                                                         <  1:257617/251‑1 (MQ=255)                                                                                                                   tcCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCAAGTCAGAAAGTTCGCCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGAGGTGCGGTGTAACAACACCAAGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCAcggcgg                                                                                                                        >  2:578574/1‑251 (MQ=255)                                                                                                                          ccAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGCCATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAAcc                                                                                                                 >  1:292199/1‑251 (MQ=255)                                                                                                                            aaaGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAAcc                                                                                                                 >  1:133431/1‑249 (MQ=255)                                                                                                                            aaaGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAAcc                                                                                                                 >  2:277390/1‑249 (MQ=255)                                                                                                                                ctgctgATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCg                                                                                                           >  2:180276/1‑251 (MQ=255)                                                                                                                                  gttgATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCGGAAAGTTCGTCCATCATCTGACCGAAAGCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCGTTGGTAATGGGGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGTTACGGCGGCAAAACCAGCCCgg                                                                                                          <  2:435439/248‑1 (MQ=255)                                                                                                                                     gATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCg                                                                                                        <  1:228723/249‑1 (MQ=255)                                                                                                                                     gATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAg                                                                                                      <  1:34632/251‑1 (MQ=255)                                                                                                                                        aataCGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGcc                                                                                                    <  2:297871/250‑1 (MQ=255)                                                                                                                                              cagcaTCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTGTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAGCGCTCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCa                                                                                             <  1:237319/251‑1 (MQ=255)                                                                                                                                                  aTCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCGCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGTGAGCCACCAGCATaaa                                                                                         <  1:194954/251‑1 (MQ=255)                                                                                                                                                    ccccTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATaa                                                                                          >  1:334831/1‑248 (MQ=255)                                                                                                                                                       cTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGAcgc                                                                                    >  1:82915/1‑251 (MQ=255)                                                                                                                                                         gCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACCCCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACgcgc                                                                                  <  2:392573/251‑1 (MQ=255)                                                                                                                                                            gCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCaaa                                                                               <  1:56330/251‑1 (MQ=255)                                                                                                                                                              cccTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATc                                                                             <  1:558318/250‑1 (MQ=255)                                                                                                                                                                       aCGTCAGAAAGATCGTCCATCATCTGACCCACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGCTGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCCAAACCACCCCGGCGAGCCACCAGCATAAAAACGCGCAAATCATTcagc                                                                      >  2:485768/1‑249 (MQ=255)                                                                                                                                                                         gTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCcgcg                                                                  >  2:175481/1‑251 (MQ=255)                                                                                                                                                                             gAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTaa                                                              <  2:161368/251‑1 (MQ=255)                                                                                                                                                                                                gACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCgt                                              <  1:3693/248‑1 (MQ=255)                                                                                                                                                                                                    gACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGGGtttttt                                       >  2:440808/1‑249 (MQ=255)                                                                                                                                                                                                        tCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCTGTGTAACAACCCCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGACCGGCGAGCCACCAGCATAAAGACGCGCAAATAATTCAGCAGCGGGAAATTATTCATGATTCGCGTTTTTTGttt                                   >  1:64423/1‑251 (MQ=255)                                                                                                                                                                                                         ccTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTc                                  <  1:511768/251‑1 (MQ=255)                                                                                                                                                                                                         ccTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTAGGTTTc                                  <  1:381796/251‑1 (MQ=255)                                                                                                                                                                                                                 aTCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTa                          <  2:110204/251‑1 (MQ=255)                                                                                                                                                                                                                   ccGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACg                        <  1:413432/251‑1 (MQ=255)                                                                                                                                                                                                                     gCTGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACCACACCACGTTTAGCGTTTGCTCCAGCAAGGCGCTGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCCTTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCCACAGTTACggg                      >  2:33701/1‑251 (MQ=255)                                                                                                                                                                                                                       tGCGCCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAAAACCACGTTTAGCGTTTGCTCCAGAAAGGCGATGCGCTTACTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCCAAACCAGACCGGCGAGCCACCAGCATAAAGAAGCGCAAATCATTCAGCAGCGGTAAATTATTAATGATTCGTGTTTTATGTTTCACCAGTTACggggt                    >  1:578308/1‑250 (MQ=255)                                                                                                                                                                                                                        gcgcCCATTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCTGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACTCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCATCAGTTACGGGGAt                   <  2:532129/251‑1 (MQ=255)                                                                                                                                                                                                                           cccATTCATAAATCCGCTCTCCTGCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTaa                <  2:133431/251‑1 (MQ=255)                                                                                                                                                                                                                            ccATTCATAAACCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCGCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTGTTATGTTTCACCAGTGACGGGGATTAAt               <  2:68681/251‑1 (MQ=255)                                                                                                                                                                                                                              aTTCATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGAGGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTc             <  1:94400/251‑1 (MQ=255)                                                                                                                                                                                                                                 cATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCtt          <  2:334831/251‑1 (MQ=255)                                                                                                                                                                                                                                 cATAAATCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCtt          <  2:448808/251‑1 (MQ=255)                                                                                                                                                                                                                                 cATAAATCCGCTCTCCTTCCTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCAGAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCGCCTGTTACGGGGAGTAATTcc            <  2:377488/249‑1 (MQ=255)                                                                                                                                                                                                                                  aTAAATCCGCTATACTTCTTTGGTACTGGTGCCGCGGCGGGTGGTGCGGCGTAACACCCCCACGTTTAGCGTTTGCTCCAGCACGGCGATGCGCTTGCTGGCGCACGCCGGTGAACCGCCCAGTTCTTCCGCCACGGCGGCAAACCCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAAACCTTCAGCAGCGGGACATTCTTCCTGATTCGTGTTTTATGTTTCACCAGTTACAGGGATTAATTCCttt         >  2:289726/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaTCCGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCa      <  1:175481/251‑1 (MQ=255)                                                                                                                                                                                                                                         cGCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCa  <  1:277390/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCTCTCCTTCTTTGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTAAGGGGGTTAATTCCTTTTCAGTCa  >  1:309655/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              GAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCA  >  NC_000913/1881378‑1881860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑