| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,878,855 | C→T | A28V (GCT→GTT) | yoaK → | putative membrane protein YoaK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,878,855 | 0 | C | T | 96.3% | 81.5 / ‑5.9 | 27 | A28V (GCT→GTT) | yoaK | putative membrane protein YoaK |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (14/12); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTGAAGCCATCGACT > NC_000913/1878617‑1879094 | agaaagcGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTTCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTGTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAg < 1:431203/244‑1 (MQ=255) gAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAAGCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCa < 2:214325/251‑1 (MQ=255) gATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTTCCCCGGGAGCATaa > 2:371264/1‑248 (MQ=255) aaaCAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCTCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGaaaaa < 1:297249/251‑1 (MQ=255) ccGATGCAGACAGGGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACAGGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGACGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAaacaac < 1:201609/251‑1 (MQ=255) cGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCTCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGGTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACg < 2:231921/251‑1 (MQ=255) ccggCATGATGACGATATTGCGATGTATCAGGAGATAAAACGCAAACATGAAACACCTCTTGGCGCGCATCCAGCGCGACATTCCTGAGGCGTATGCACATCCTGATGATTGGTTAGAACATGAGAATGGGTATTATTTTTCCGGTTGTAATCGTGATTACATCGGTCGTATGTTTAGCATCGTTTTTTATTGGCGGCGGAGCTTCCCCGGGCTCATAAAGATGAAAAAAACAACGATTATTATGATGGgt < 1:363973/248‑1 (MQ=255) gATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTc > 1:262698/1‑251 (MQ=255) aaaaaaCACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGGGCATAAAGATGAAAAAAAACACGATTATTATGATGGGGGGTGCGTTTATTTGCGTACTCGGCACTggggtgg > 1:15314/1‑245 (MQ=255) gTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGAACTCGGCACTGAACTGGGGTGGGGGGAACGTCACCTCTaaaaa > 2:107205/1‑250 (MQ=255) gcgcATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTTAAAAATa > 1:242126/1‑250 (MQ=255) gcATCCAGCGATACATTCCTGAGCCGTATTCACATCCTTTTGATTGGCGATAACATGCGCATCGGTATTATTTTTCCGGTTGAAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGTCCCGGGGGCATAAAGATGCAAAAAACAACAGTTATTATGATGGGGGGGGGGCTTTTTTTCGTACTCGGCCCTGAGATGGGATGGTGGTAACGGCCCCTCTAAAAAAtcac > 2:256449/1‑247 (MQ=255) aTTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGTGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCTCCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTtgtg < 1:312802/250‑1 (MQ=255) caTCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGTCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCGCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAAttt < 1:256449/251‑1 (MQ=255) caTCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAAttt < 2:262698/251‑1 (MQ=255) ttGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGt < 1:186926/250‑1 (MQ=255) gATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGGGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCCGCCTTTGTGCAATTTAAGAGTTa > 2:419224/1‑250 (MQ=255) cATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTc < 1:571448/251‑1 (MQ=255) cATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGAATAAAGATGAAAAAAACAACGATTATTTTGATGGGGGTGGCGATTTTTGTCGTACTCGGCCCTGAGCTGGGAGGGGGGTAAAGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGAAGCCTTTGTGACATTTAAACGGTAACTTTTAATCttt > 2:571448/1‑250 (MQ=255) tGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGGGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCt > 1:404115/1‑251 (MQ=255) aTCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCTGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGAGTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAAGCTTCCTGTAGa < 2:80198/251‑1 (MQ=255) ttGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGc > 1:437902/1‑251 (MQ=255) gTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGGTGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCAc > 1:78909/1‑251 (MQ=255) tAATCTACATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTAAGTTGTACCGTGATTATAAAGATGTAATAAATACCGTTTATTTTAATGGTGGTGGCGAATAATGTCGTACACGGCACTGAACTGGGGTGGTGGTAACGTGACCTCTATAAAATAGCCAAGGCTGTCCGTGGGCAGCCTTTTTGCCATTTAAACATTAACTATTAGTCTTTCTGTCGATAAATataccaaaaagctcaac > 2:171987/1‑235 (MQ=255) cGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGaa < 1:86780/250‑1 (MQ=255) ttGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTGAAGCCATCGAc > 2:247160/1‑251 (MQ=255) tGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTGAAGCCATCGACt > 1:433019/1‑251 (MQ=255) | AGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTGAAGCCATCGACT > NC_000913/1878617‑1879094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |