Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,876,099	C→T	L143L (CTG→TTG)	nimT →	2‑nitroimidazole exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,876,099	0	C	T	98.4%	203.5 / NA	64	L143L (CTG→TTG)	nimT	2‑nitroimidazole exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/35);  minor base G (0/1);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAAC  >  NC_000913/1875852‑1876341                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     ggtcTTTGCGCTAATCTCTCCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTAGGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTTTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTGTGCGGTACAGCGGTCATTGGCTGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGa                                                                                                                                                                                                                                                   <  2:432994/248‑1 (MQ=255)      ttGCGCTAATCTTACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATGCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAAt                                                                                                                                                                                                                                               <  1:115922/250‑1 (MQ=255)             aaTCTCACCGTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCt                                                                                                                                                                                                                                        <  2:457556/250‑1 (MQ=255)                             gCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATc                                                                                                                                                                                                                      <  2:164189/251‑1 (MQ=255)                                     aGCGCGACGTTTTGGTATGGATCGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCAGTCCGTCGCCAGACTTATCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGTCTATg                                                                                                                                                                                                               <  2:34493/250‑1 (MQ=255)                                           aCGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAGGAAATGTCTTTCTGCCAGGATTAATTAAACGCGCTTTCCCTCATTTCGTCGCCCAACTTACCGGCGCATATTCCTTTACAATGGGGGCTGCAGGGGccatggggtc                                                                                                                                                                                                                       >  1:552887/1‑228 (MQ=255)                                           aCGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTAACGCAATTCGCTCTCGCCCTTCGCCTTACTTATTATTTGGCGGGACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACGGCCAGGATTAATTAAACGCGATTTCCCTCAGTCCGTCGCCAGACTTACCGGCGCATATTCCTGGACAATGGGAGCGGCAGCGGCACTGGG‑ATc                                                                                                                                                                                                                      <  2:552887/237‑1 (MQ=255)                                                      tGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTTCTTTAGGCAATTTCTTTCTGCCAGGCTTTATTAAACGCGATTTTCCTCATTTCTTCGCCAGACTTTACGGAGCATATTTCTTTACCATGGGAGCTTCCGCGACACTGGGGAT‑GGCTATGTTCGTCCCggcggcgttg                                                                                                                                                                                             >  2:214020/1‑242 (MQ=255)                                                           cGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACgg                                                                                                                                                                                       <  2:19381/251‑1 (MQ=255)                                                             tAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGtt                                                                                                                                                                                     >  1:157300/1‑251 (MQ=255)                                                              aGCCTGTTTTCCGCGTTACTGTTGATCTGTGCTGGTATCGCAATTCTCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCGTT‑GATCGGtt                                                                                                                                                                                     <  2:19705/250‑1 (MQ=255)                                                                  tGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTTCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGATATGGTCGTGCCGCTGGCTTT‑GAACGGTTTtggg                                                                                                                                                                                >  1:328093/1‑250 (MQ=255)                                                                              tACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGgcgc                                                                                                                                                                     <  2:207073/250‑1 (MQ=255)                                                                               tcTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCCTCGCGTTACATATTATTTGGCGGTGAAGCGGTCCTTGGCTGTGGGATTGCATTAGGCAAGCTCTTACTGCCAGGATTAATTAAACGAGGTTTCCCTCATTCCGGCGCCAGACTTGCCGACGCATATTCCTAGACAATGGGAGCTGCAGAGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGATTT‑GACCGGTTGTGGCTGGCAAGGcgcg                                                                                                                                                                    <  2:348627/249‑1 (MQ=255)                                                                                  tttGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGtt                                                                                                                                                                  <  2:544918/249‑1 (MQ=255)                                                                                    tGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCCTTTGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCCGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGGTTT‑GAACGGTTTTGGCTGGCCAGGCGCGTTGCTc                                                                                                                                                              >  2:571905/1‑251 (MQ=255)                                                                                    tGATCTGTGCTGGTATCGCAAGTCGCTCTCTCCCTTCGCCGTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAACGGGAGCTGTAGCGGCACTGGG‑ATCGGCTATGGTCGTGTCGCTGGCTTT‑GAACGGTTTTGGCTGGCACGGCGCGTTGCt                                                                                                                                                               <  2:432427/250‑1 (MQ=255)                                                                                          gtgCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTGCAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTg                                                                                                                                                        <  2:136695/251‑1 (MQ=255)                                                                                          gtgCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGc                                                                                                                                                          <  2:142323/249‑1 (MQ=255)                                                                                            gCTGGTATCGCAATTCGCTCTCTCCCTTCCCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCCTTATGCAATTTCTTCCTGCCAGGGTTAATTTAACCCGGTTTCCCTCCTTCCGTCGCCCGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCCCTGGG‑GTCGGCTTTGGTCGGGCCGCTGGCCTT‑GAACGGGTTTGGGTGGCAAGGCGCGTTGCTCATGCTGAt                                                                                                                                                      >  1:314009/1‑251 (MQ=255)                                                                                               ggTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGTTTGCATTAGGCAATGTCTTACTGCCCGGGTTTATTAAACGCGGTTTCTCTCATTCCGTCGCCCGAATTACCGGCGCATATTTCTTGACAATGGGGGCAGCAGCGGCCATGGG‑ATCGGCTATGGTTGTGCCGCTGGCTTTTGTA‑GGGTTTGTCGTGCAAGGCGCGTTGTTCATGCTGAtgtg                                                                                                                                                   >  2:91961/1‑251 (MQ=255)                                                                                               ggTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGAtg                                                                                                                                                     <  1:61104/249‑1 (MQ=255)                                                                                                           cgcccgctcCCGTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGAGTTCCCTCATTCCGTCGACAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGTG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTctgc                                                                                                                                        <  2:331634/244‑1 (MQ=255)                                                                                                           cGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCGTctgc                                                                                                                                        <  1:3760/250‑1 (MQ=255)                                                                                                                tctcCCATCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTAGCATTATGCAATGTCTTACTGCCAGGATTAATTTAACGCGAGTTACCTCATTCCGTCGCCCGACTTCCCGGGCCCTATTCCTTGTCACTGGGAGCTGCAGCCGCACTGGG‑ATCGGCTCTGGTTGGGCCGCTGTGTTT‑TAACGGGTTTGGCAGGGTAGGCGCGGTGCGCATGCTGGTGGGGTTTCctttgttggatc                                                                                                                                  >  2:446454/1‑241 (MQ=255)                                                                                                                   cccTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCttt                                                                                                                               <  1:397441/251‑1 (MQ=255)                                                                                                                    ccTTCGCCTTACTTATTATTTGGCGGTACAGCGGTTATTGGCTGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGTTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCtttt                                                                                                                              <  1:243792/251‑1 (MQ=255)                                                                                                                            ttacttaTTATTTGGCGGTACCGCGGTCATTGGCGGTGGGGTTGACTTTGGCAATGTCTTTCTGCCAGGATTTATTACACGCGGTTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCCGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGGTTT‑GAACGGTTTTTGGTTGCAAGGCGGGGTGGTCATGCTGCTGTGTTTTTCTCTGCTGGCTCTTTTTTTTTggg                                                                                                                      >  2:292951/1‑250 (MQ=255)                                                                                                                             tacttaTTATTTGGCTGTACAGCGGTCATTGGCTGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGc                                                                                                                      <  1:446454/250‑1 (MQ=255)                                                                                                                             tacgtattatTTGGCGGTACAGCGGTCATTGGCGGGGGGATTGCATTAGGCTATGTCTTACTGCCAGCATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACTGTTTTGGCTGGCAAGGCGCGTTTCTCATGCTGATGTGTTTTCCTCTTCTGGCTCTTTTTCTAttgc                                                                                                                      <  1:446446/246‑4 (MQ=255)                                                                                                                                 tattatTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGcc                                                                                                                  <  1:562613/250‑1 (MQ=255)                                                                                                                                             gTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGt                                                                                                     <  1:292951/251‑1 (MQ=255)                                                                                                                                                   cGGTCATTGGCGGTGGGATTTCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTcaacaa                                                                                               <  1:502909/251‑1 (MQ=255)                                                                                                                                                       cATTGGCGGTGGGATTGGATTAGGCAATGTTTTACTGCCAGGATTAAGTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGGAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGGGCATGCTGATGTGTTTTCCTCTGCTTGGGCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATg                                                                                           <  2:296896/251‑1 (MQ=255)                                                                                                                                                       cATTGGCGGCGGGGATGCATTAAGCAATGTCTTACTGCCAGGATTTAATAAACGCGATTTCCCTCATTCCGTCGACAGACCTACCGGCGCCTTTTCCTTGACAATGGGAGCTGCCGCGGCCCTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GCACGGTTTTTGCTGGCAAACCGCGTTGCTCATGCTGGTTTGTTTTCCTCTGCTGGCTCTTTTTTTTTGGCTGCCCCAGTTGCGGAGTCAACAACATg                                                                                           >  2:212522/1‑251 (MQ=255)                                                                                                                                                                tGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAACCGCGATTTCCCTCATTCAGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGATGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCTTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTT‑ATGGCTGCCACAGGGGCGAATGCAACAACCTGCAAATTTGat                                                                                 >  2:507068/1‑250 (MQ=255)                                                                                                                                                                  ggATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGt                                                                                <  1:395196/251‑1 (MQ=255)                                                                                                                                                                       gCATTAGGCAATGGCTTACTGCCAGGATTAATTAAACGCGCTTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATGCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTTAGTACCTc                                                                           <  2:418061/251‑1 (MQ=255)                                                                                                                                                                        cATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTcg                                                                          >  1:355477/1‑251 (MQ=255)                                                                                                                                                                           tAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTcgcgc                                                                       >  1:89481/1‑251 (MQ=255)                                                                                                                                                                            aGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGCAGTAAACAACATGAAAATTTGAGTACCTcgcgtg                                                                      >  1:404774/1‑249 (MQ=255)                                                                                                                                                                                       tACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTGACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCGCTGTG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATa                                                             <  1:214020/249‑1 (MQ=255)                                                                                                                                                                                                  ttaattaaACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACCTCCTCGGGGTATCTg                                                >  2:133864/1‑251 (MQ=255)                                                                                                                                                                                                        aaaCGCGATTTCCCTCATTCCGTCGCCCGACTTACCGGCGCATATTCCTTGACAATGGGCGCTGCAGCGGAACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTc                                          >  2:217008/1‑251 (MQ=255)                                                                                                                                                                                                        aaaCGCGATTTACCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCACGCGCCTTACATACTCGGGGTATCTGGCGTTc                                          >  2:525227/1‑251 (MQ=255)                                                                                                                                                                                                         aaCGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCa                                         <  1:380469/251‑1 (MQ=255)                                                                                                                                                                                                              gATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCt                                    >  1:575567/1‑251 (MQ=255)                                                                                                                                                                                                               aTTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCtt                                   >  2:347169/1‑251 (MQ=255)                                                                                                                                                                                                                 ttCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTg                                  <  1:122275/250‑1 (MQ=255)                                                                                                                                                                                                                 ttCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGAGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGc                                 >  1:500339/1‑251 (MQ=255)                                                                                                                                                                                                                   cccTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGcc                                <  2:439688/250‑1 (MQ=255)                                                                                                                                                                                                                    ccTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCGGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTg                              <  2:202117/251‑1 (MQ=255)                                                                                                                                                                                                                      tCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGc                            >  2:430818/1‑251 (MQ=255)                                                                                                                                                                                                                             gTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCCGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGAGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCAGCCACAGTGGAGAAGTCAACAACATGCAAATTTGAGAACCTCGCACGCCTTACATACCCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTcac                     >  2:495666/1‑251 (MQ=255)                                                                                                                                                                                                                               cgcaaGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGGCGTGCCGCTGGATTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTTCTGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGCAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGAGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACAt                   >  1:240296/5‑251 (MQ=255)                                                                                                                                                                                                                                 ccAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACCACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTg                 >  2:38925/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccAGACTTACCGGAGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGGTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACAAGCAAATTTGAGTACCTCGCGCGCCTTACATCCACGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGACACATTg                 >  2:381867/1‑251 (MQ=255)                                                                                                                                                                                                                                      cTTACCGGCGCATATTCCTTGACAATGGGCGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCt            >  1:187683/1‑251 (MQ=255)                                                                                                                                                                                                                                        tACCGGCGCATATTCCGTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCt            <  1:212522/249‑1 (MQ=255)                                                                                                                                                                                                                                         aCCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGTGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTg          <  1:462613/250‑1 (MQ=255)                                                                                                                                                                                                                                          ccGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTggg        <  1:91961/251‑1 (MQ=255)                                                                                                                                                                                                                                            ggCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATAGGATATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGTAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTggggt      >  1:501505/1‑249 (MQ=255)                                                                                                                                                                                                                                                cATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTTCCGCTGGCTTT‑GAACGGTTTTGGCCGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAAc  <  1:131734/251‑1 (MQ=255)                                                                                                                                                                                                                                                cATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGCGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGATCATGCTGATGTGTTTTCATCTGCTGGCTCTTTTTTTATGGCTGCCACCGTGGCGAAGTCCACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACACAGGGCATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGGTTCTTGGGATCAAc  >  1:345521/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGG‑ATCGGCTATGGTCGTGCCGCTGGCTTT‑GAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAAC  >  NC_000913/1875852‑1876341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑