Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,420	C→T	G18D (GGC→GAC)	mipA ←	MltA‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,420	0	C	T	100.0%	100.9 / NA	32	G18D (GGC→GAC)	mipA	MltA‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

ACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTA  >  NC_000913/1866191‑1866647                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    aCTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGCCGTTGCGATAAGc                                                                                                                                                                                                                 >  1:187435/1‑250 (MQ=255) aCTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCa                                                                                                                                                                                                                <  2:43737/251‑1 (MQ=255)                    tAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAagttt                                                                                                                                                                                             >  1:77655/1‑251 (MQ=255)                            cgACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTgg                                                                                                                                                                                     <  1:34321/251‑1 (MQ=255)                                    aaGCGGGAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTCACCACCACCTAAGCCACGGAACCAGACGTTATAGCATTCATACTTGATTACCGGGACTGGGTAAACATCGGAATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCCGCAGACGTGGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTGACAAtt                                                                                                                                                                             >  1:170403/1‑251 (MQ=255)                                     aGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTa                                                                                                                                                                            <  1:222183/251‑1 (MQ=255)                                      gCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTaa                                                                                                                                                                           <  1:22142/251‑1 (MQ=255)                                      gCGGTAATGGAAAGTTTATCCGTTGCGTCAGTCCACAGGTAGTAACCTCCACCTAAGCCACTGAACCAGAAGTTATCGCCTTCATAGTTGAGTACCGGTACAGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGTAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCTATAATCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTaa                                                                                                                                                                           <  2:530376/251‑1 (MQ=255)                                               gAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCAACTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCtt                                                                                                                                                                  <  1:85277/251‑1 (MQ=255)                                                    tttATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTCCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAAc                                                                                                                                                              >  1:63336/1‑250 (MQ=255)                                                      tATCCGTTGCGTCATTCCACAGGAAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACAGGTACTGCGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGACACAATTAATCATTCCTTAACCaa                                                                                                                                                            >  1:527374/1‑250 (MQ=255)                                                          cGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGtt                                                                                                                                                       <  1:451273/251‑1 (MQ=255)                                                                gTCATTCCACAGGTAGTAACCACCCCCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCgg                                                                                                                                                 >  2:120620/1‑251 (MQ=255)                                                                    ttCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCg                                                                                                                                              >  1:425207/1‑250 (MQ=255)                                                                            gtagtaACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATGCCTTAAACAAATGTTTAGCGGGCGACAAAGttt                                                                                                                                     <  1:475740/251‑1 (MQ=255)                                                                                    caccatcTAAGCCACGGAACCAGAAGTTATCGCCTGCATAGTTGATTACCGGTACGGGGTAAACATCGGTATCGTAATCTTTATATTTGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATCTACCTGCAGCGTGCGCTTCGTCTGCAGACGTTTCGATAAGCACTCCAAGTGCCTGAAGTGTGAGTTTGGTCGCAATTAACCATTCCTTAAACAAATTTTTAGCGGGCGACAAAGTTTTCCCGTCa                                                                                                                             <  1:27050/251‑1 (MQ=255)                                                                                               ccACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGaa                                                                                                                   >  1:232624/1‑250 (MQ=255)                                                                                                     aaCCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACAGCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAACCAAATGTTTAGCGGGCGACAAAGTTTAACCGTCAATACATCGAAACCCAAc                                                                                                            >  2:307783/1‑251 (MQ=255)                                                                                                                        cATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCAtttt                                                                                         >  2:118025/1‑251 (MQ=255)                                                                                                                                   aCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACt                                                                              >  2:415253/1‑251 (MQ=255)                                                                                                                                              gTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTc                                                                   <  1:576309/251‑1 (MQ=255)                                                                                                                                                        gTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGAcgc                                                            <  1:259082/248‑1 (MQ=255)                                                                                                                                                        gTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGAcgc                                                            >  2:259082/1‑248 (MQ=255)                                                                                                                                                          aTCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGAt                                                        <  1:118025/250‑1 (MQ=255)                                                                                                                                                          aTCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATg                                                       <  2:232624/251‑1 (MQ=255)                                                                                                                                                            cGTAATCTTTATATGGGTGTTCAACGACGCCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGGTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGac                                                     <  1:56771/251‑1 (MQ=255)                                                                                                                                                               aaTCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTGTCCTGACGCGGATGacaa                                                   <  2:11412/250‑2 (MQ=255)                                                                                                                                                                   tttATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCgcgc                                              >  2:234584/1‑251 (MQ=255)                                                                                                                                                                                  aaCGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGa                                >  1:106940/1‑250 (MQ=255)                                                                                                                                                                                                            aaaaTTTACCTTCAGCGTGCGCTACGTCTGCGGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCt     >  1:44536/1‑251 (MQ=255)                                                                                                                                                                                                               aTTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCttt   >  1:217100/1‑250 (MQ=255)                                                                                                                                                                                                               aTTTACCTTCAGCGTGCGCTACGTCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTa  <  1:14549/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    ACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTA  >  NC_000913/1866191‑1866647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑