Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,977	C→T	M1M (ATG→ATA) †	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,977	0	C	T	96.5%	97.0 / NA	29	M1M (ATG→ATA) †	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/18);  minor base G (0/1);  total (10/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCACCAATAAACCCACCAGTAA‑GTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTGGACAAAAGCTTTGCGAT  >  NC_000913/1853733‑1854217                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 agtCACCAATAAACCCACCAGTAA‑GTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAg                                                                                                                                                                                                                                             <  2:327813/250‑1 (MQ=255)         aTAAACCCACCTGTAA‑GAGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGCCATTACCGGCGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGCTCATGACACAGGGCCAAAAGCAAGCGGTACATTCTTGCGCCCATTAGATCATATTGTTATATTAGGTGCCTCgg                                                                                                                                                                                                                                    >  2:18944/1‑251 (MQ=255)         aTAAACCCAC‑AGTAAAGTAGGCCGATGAAAAAACAAAACATTAAGGCCGAGGTAAAGGCGCCATGAAGAAAATTATTTGCCAATCCATGCCTTACCAGCTTTGCAAAGACGCCATTACCG‑AGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGa                                                                                                                                                                                                                                   <  1:40013/251‑1 (MQ=255)                       aa‑GTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTCCCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTAAAATACCAAATATACGATAATTAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATGTGCTAAAAAgt                                                                                                                                                                                                                      <  2:24465/251‑2 (MQ=255)                                     aaaTAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGc                                                                                                                                                                                                         <  1:313107/251‑1 (MQ=255)                                      aaTAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCa                                                                                                                                                                                                        <  1:391095/251‑1 (MQ=255)                                                              tAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACtt                                                                                                                                                                                               >  1:502635/1‑236 (MQ=255)                                                              tAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACtt                                                                                                                                                                                               <  2:502635/236‑1 (MQ=255)                                                                    cggcATTGAGGAAATTATTTGACCATCCATTGCTTACCCGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCAttt                                                                                                                                                                          >  2:97202/1‑251 (MQ=255)                                                                    cggcATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCAttt                                                                                                                                                                          <  2:370927/251‑1 (MQ=255)                                                                          tGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTc                                                                                                                                                                    >  1:84534/1‑251 (MQ=255)                                                                                    attTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAgg                                                                                                                                                          >  2:424543/1‑251 (MQ=255)                                                                                      ttGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGa                                                                                                                                                         <  1:389516/250‑1 (MQ=255)                                                                                          ccatccatTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACaaa                                                                                                                                                    <  2:544297/251‑1 (MQ=255)                                                                                                        ccAGCTTTGCTAAGACGACAATACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATCCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTGCAAGGCGCAGGAACAAATAATATTGACAACg                                                                                                                                      <  2:138517/251‑1 (MQ=255)                                                                                                              ttGCTAAGACGAAATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGtgtg                                                                                                                                  <  1:81766/249‑1 (MQ=255)                                                                                                                gCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATAGACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCtt                                                                                                                               <  1:496803/250‑1 (MQ=255)                                                                                                                             tACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACAcc                                                                                                                  <  2:425265/250‑1 (MQ=255)                                                                                                                                cGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCt                                                                                                              <  2:121004/251‑1 (MQ=255)                                                                                                                                                       aataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGAGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAAt                                                                                       >  1:77248/1‑251 (MQ=255)                                                                                                                                                                   aaGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCActct                                                                           >  2:174188/1‑251 (MQ=255)                                                                                                                                                                     gCTTATAATACCAAATATACGATATTGAAAACGGGCCAAAGGCAAGCGGGCCAGTCTTGCGCCAATTTGATCATAGTGTTCTATTAGTTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCGTTAGCATCATTTTATTTCAAGGCGCAGGATCAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCGGGATATTATTGCCTGTGCAAAATGCTATCCACTCTGc                                                                         <  2:587099/251‑1 (MQ=255)                                                                                                                                                                           aataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTCGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCgg                                                                   >  2:165620/1‑251 (MQ=255)                                                                                                                                                                             taCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTg                                                                 >  2:43241/1‑251 (MQ=255)                                                                                                                                                                                       acgctAATGAAACCGTGCCAAAGGGCAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCGATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGAAACAAATAATAGTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTACTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAg                                                        <  2:385153/246‑1 (MQ=255)                                                                                                                                                                                        cGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGACGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTTGATATTATTGTCTTTGCAAAATGCTATCCACACTGCACCCGGTGATTTATCAGtt                                                      <  2:461995/251‑1 (MQ=255)                                                                                                                                                                                          aTAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTg                                                                          <  2:379692/229‑1 (MQ=255)                                                                                                                                                                                          aTAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTg                                                                          >  1:379692/1‑229 (MQ=255)                                                                                                                                                                                                                                            cATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTGGACAAAAGCTTTGCGAt  <  1:424543/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 AGTCACCAATAAACCCACCAGTAA‑GTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTGGACAAAAGCTTTGCGAT  >  NC_000913/1853733‑1854217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑