Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,057	C→T	G308D (GGC→GAC)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,057	0	C	T	100.0%	70.6 / NA	23	G308D (GGC→GAC)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

AAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTC  >  NC_000913/1852811‑1853301                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aaaCCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAAt                                                                                                                                                                                                                                                  >  2:324905/1‑251 (MQ=255)   aCCCGAACCGCGCAGGCGTAAATGCGTTGGCAAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCa                                                                                                                                                                                                                                                <  2:170134/251‑1 (MQ=255)          ccGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAAc                                                                                                                                                                                                                                         >  1:292078/1‑251 (MQ=255)                        aTGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAata                                                                                                                                                                                                                           <  2:261939/251‑1 (MQ=255)                                 ccAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATACCAGCGGTCATTAATATTGATTTAt                                                                                                                                                                                                                  >  2:444567/1‑251 (MQ=255)                                              atatAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGcc                                                                                                                                                                                                     >  1:139963/1‑251 (MQ=255)                                                                          acatGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCAt                                                                                                                                                                         <  1:180833/251‑1 (MQ=255)                                                                                                    cgTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGtt                                                                                                                                               >  2:191150/2‑251 (MQ=255)                                                                                                           tgattacaatagacaattctGTGGTCTGAAATGAATAGATATAGCCTAACACGGCCATAATAATACGTAAGGTGGAGCCAAATAACCGACGAGGGAAATGATCAATAATTAATGCCGCAATAAATATACCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATATATCGACATCAATACCGGAGTTAACAAATATGGTCGGTATCCATAGGGTGAGGGTATAAAGAGACATGTTCATTGcc                                                                                                                                        >  2:405056/18‑250 (MQ=255)                                                                                                           aGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGTTATAGCCTAACACGGCAATACTAATAAGGAAGGAGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCACAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTACCAACTATGGTCGGTATCCATACGGTGATGGTATACAGCGAAATGTTCCTTGc                                                                                                                                         >  2:308847/1‑250 (MQ=255)                                                                                                            gATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCa                                                                                                                                        >  2:82928/1‑250 (MQ=255)                                                                                                                     cGCCCACGCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTAGCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACa                                                                                                                               <  1:370557/250‑1 (MQ=255)                                                                                                                                             atAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGACGCAGTAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTAcgtcgt                                                                                                       >  1:499454/1‑250 (MQ=255)                                                                                                                                                                       taataaGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGcc                                                                              >  1:60079/1‑249 (MQ=255)                                                                                                                                                                               aagGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTAcc                                                                    >  1:129407/1‑251 (MQ=255)                                                                                                                                                                               aagGGGGAGCCAAATAACCGACGAGGAAAATGATCAAGAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGTCATCAATGCCGGAGTTAACAAATATGGTCGGTAGCCATACGGTGATGGTATAAAGCGGAATGTTCATTTCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTAcc                                                                    <  1:378240/251‑1 (MQ=255)                                                                                                                                                                                    ggAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTg                                                                <  1:313727/250‑1 (MQ=255)                                                                                                                                                                                              aaCCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTaa                                                      <  1:11647/250‑1 (MQ=255)                                                                                                                                                                                                 cgacgaGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCtt                                                  <  2:139963/251‑1 (MQ=255)                                                                                                                                                                                                  gacgaGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGAATTATCGACAGCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTTCGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAAccatg                                                 <  2:147362/251‑4 (MQ=255)                                                                                                                                                                                                                            gCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCg                        <  1:506752/250‑1 (MQ=255)                                                                                                                                                                                                                                           ccTACCGGAGCGTCACTCATATCAACATCTGTCATTAATATTTATTTATCGACATCAATGCCGGATTTAACAAATATGGTCGGTATCCATACGGTGATGTTATAAAGCTAAATGTTCATTGCAATTATAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTCACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGTGTAAACGAATAct        <  1:302575/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ggAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTtctc  >  1:38747/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     AAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTC  >  NC_000913/1852811‑1853301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑