Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,847,969	C→T	G14R (GGA→AGA)	selD ←	selenide, water dikinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,847,969	0	C	T	97.7%	147.4 / NA	44	G14R (GGA→AGA)	selD	selenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/24);  minor base G (0/1);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGAC  >  NC_000913/1847725‑1848214                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aTCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAACGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGCAATTTTACAGCCGCAACCAGCTCtgtgtct                                                                                                                                                                                                                                                 >  1:512768/1‑248 (MQ=255)        tgATATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTAATGCTAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACACGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACCCTTTTGGGGAAATTTTACCGCCGCAACCAGCTCTGTGGCTGTCTTgg                                                                                                                                                                                                                                          >  2:10556/2‑251 (MQ=255)                               gccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCt                                                                                                                                                                                                                   >  1:9094/1‑251 (MQ=255)                                ccgcAATGCGGCCAAAATCGAAAGGATTATTACCGACCGGCATAAATAAGCCGGTGCTACTGATAACTCTTGGGCCATTGCCCAGATCGCACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAACCTTCGCCTGCTCACTATGCAGGATGTTTTCCATCACTTTTGGGGAAATTTTACAGCCGGAACCAGCTCTGTGGCTGTATTGGGTTAAACGAATCGAGTTCTCGCTc                                                                                                                                                                                                                  <  1:353442/251‑1 (MQ=255)                                       gCGGCAAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCCCAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGACTCGAGTTCTCGCTCATGGAc                                                                                                                                                                                                            >  2:92707/1‑250 (MQ=255)                                                     aaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGc                                                                                                                                                                                             >  1:244834/1‑251 (MQ=255)                                                        gATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAAt                                                                                                                                                                                          >  1:318511/1‑251 (MQ=255)                                                          ttATCAGCGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCCCTCATGTACATCTCCTGTCAATGCAATcc                                                                                                                                                                                        >  2:100642/1‑251 (MQ=255)                                                                     gcccaaaaagaagtgggggtcccTGATAGCGCTGGTTCCGTTCCCCAGCCAGTACACCGCCGCATCGTCGCGGTTTTCATTACCCACAAGCAAATTCGGCCCAACAAACTTCGCCGGCCCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAAGTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGCTCAAACGAATCGAGTTCTCGCTCATGGACATCGCCTCTCAATGCAATCCGGTTATGGt                                                                                                                                                                               <  2:96864/227‑1 (MQ=255)                                                                     cGGCTTAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTa                                                                                                                                                                              <  1:420622/250‑1 (MQ=255)                                                                               aaggcGGTGTTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTcc                                                                                                                                                                   <  1:508876/247‑1 (MQ=255)                                                                                                      ggTGCCATTGCCAATATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTc                                                                                                                                            <  1:323340/251‑1 (MQ=255)                                                                                                       gTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCt                                                                                                                                           <  1:401766/251‑1 (MQ=255)                                                                                                        tcccatgccccAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGGCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCt                                                                                                                                           <  2:332010/242‑1 (MQ=255)                                                                                                         gCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTg                                                                                                                                          >  1:399343/1‑250 (MQ=255)                                                                                                            aTTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAAtt                                                                                                                                      >  1:231/1‑251 (MQ=255)                                                                                                            aTTGCCCAAATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCAGTCCGTGAAGTGATAAGTGAGAGTGTCTGAAtt                                                                                                                                      <  1:484742/251‑1 (MQ=255)                                                                                                               gcccagaccttccacCGCCGCATCGTCGCGTGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTTAATTcc                                                                                                                                    <  2:65238/238‑1 (MQ=255)                                                                                                                       cGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCtt                                                                                                                            <  1:385365/250‑1 (MQ=255)                                                                                                                                           cgGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGttt                                                                                                       >  1:14175/1‑251 (MQ=255)                                                                                                                                                ttCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTcgcg                                                                                                   <  2:223709/250‑1 (MQ=255)                                                                                                                                                     tACCCACAAGCAAATTCGGATCAACACACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAAt                                                                                                                                                                                          >  2:278365/1‑158 (MQ=255)                                                                                                                                                     tACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAAt                                                                                                                                                                                          <  1:278365/158‑1 (MQ=255)                                                                                                                                                       cccACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTa                                                                                            <  2:105913/250‑1 (MQ=255)                                                                                                                                                        ccACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATc                                                                                          >  2:538893/1‑251 (MQ=255)                                                                                                                                                          acaAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCCATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGCAAGTTTCGCGACAATTATc                                                                                          >  2:126666/1‑249 (MQ=255)                                                                                                                                                            aaGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATTGTCGCCAACACTTTGGGGGAAATTGTACAGCCGCAGCCAGCTCGGTGGCTGTAGTTGGTCAAACGAATCGAGTTCTCGCTCATGGACATCGCCTGTCTATGCAATCCGGGGATGGTAACCCGCATTCCGTGAAGTGAGAAGTGAGAGTGTCGGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGaa                                                                                      <  2:534011/251‑1 (MQ=255)                                                                                                                                                              gCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACATCTTAGCGCCAATTATCAGaact                                                                                    >  2:457176/1‑249 (MQ=255)                                                                                                                                                                 aaTTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTTTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGAGAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAg                                                                                 <  1:560042/251‑1 (MQ=255)                                                                                                                                                                            acaaACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGc                                                                      <  1:348763/251‑1 (MQ=255)                                                                                                                                                                               aaCTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTc                                                                   <  2:9094/251‑1 (MQ=255)                                                                                                                                                                                              cTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGtt                                                     >  1:79668/1‑250 (MQ=255)                                                                                                                                                                                                aTGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGTCGTCGGGGCCGTGACGTTAAt                                                  <  1:126666/251‑1 (MQ=255)                                                                                                                                                                                                            ttCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGc                                      >  2:326415/1‑251 (MQ=255)                                                                                                                                                                                                            ttCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGc                                      >  1:547682/1‑251 (MQ=255)                                                                                                                                                                                                               cAACACTTTTGTGGAAATTTTACGGCCGCAACCAGCTCTGTTGCTGTATTGGGTCAAACGAATCGAGTTCTCTCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGAGAAGTGAGAGTGTCTGAATACCTGCGCCTTTGCTCACAAGCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTCCAAACGGCGTCGTTTCCGGTACGTTAATCGACGTAAATGCttt                                   <  2:74973/251‑1 (MQ=255)                                                                                                                                                                                                                aaCACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTc                                  <  1:538893/251‑1 (MQ=255)                                                                                                                                                                                                                   acTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGCGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGCATGGTAAACCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGCATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGc                               >  2:207529/1‑251 (MQ=255)                                                                                                                                                                                                                       ttGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCAGGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTgc                            <  1:332202/250‑1 (MQ=255)                                                                                                                                                                                                                         ggggAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTgcggc                         <  2:318511/251‑1 (MQ=255)                                                                                                                                                                                                                                    aCAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTa              <  2:124135/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCAACCAGCTATCTGGCTGTGTTGGGGCCAACGAATAGAGTTCTCGATCATGGACATCTCCTGTCAATGCAATCCGGGTATGGCAACCCTCATTCCGCGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGCGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTCCCGAGGTagggga         >  2:553590/1‑246 (MQ=255)                                                                                                                                                                                                                                            aaCCAGCTCTTTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCTGCGTACCGAGGTAGAGAAAAc      <  2:387495/251‑1 (MQ=255)                                                                                                                                                                                                                                               cAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCg    >  2:210528/1‑250 (MQ=255)                                                                                                                                                                                                                                                aGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGTACAGCTCCTGTCAATGCAATCCGGTTATGGTAACCCTCAGTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTGGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTGGGGTCCGGGACGTTAATCGACGTAGATTCTGTCAGCTGCGGCGTACCGAGTTAGAGAAAACCGAc  <  2:564605/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGAC  >  NC_000913/1847725‑1848214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑