Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,844,146 C→T A242A (GCG→GCA)  ynjI ← DUF1266 domain‑containing protein YnjI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,844,1460CT97.5% 132.2 / NA 40A242A (GCG→GCA) ynjIDUF1266 domain‑containing protein YnjI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/20);  minor base G (0/1);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAACTGCTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTAAAGACTCG  >  NC_000913/1843908‑1844377
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       
aaaaCTGCTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATAtt                                                                                                                                                                                                                             >  2:124076/1‑251 (MQ=255)
 aaaCTGCTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATAtt                                                                                                                                                                                                                             >  2:525978/1‑250 (MQ=255)
      gcgtGTCGTAACTATAACACGCTTCAAGTTCAGCATCCGTTAGCTTTCTGCGATAGCTGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCTTTTCGAGAAGCAAGCATTATATAATGTCAGTCCAGTTCTTCGTCAATTGTCCCGTTTTGAAAAGCTGTGCTTTATATCGATATCATCCCTGATAATTCTGCCGCCCATATTAGCtt                                                                                                                                                                                                                        <  2:240940/247‑1 (MQ=255)
      gCTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTc                                                                                                                                                                                                                       <  2:504755/251‑1 (MQ=255)
       cTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCg                                                                                                                                                                                                                      >  1:183218/1‑251 (MQ=255)
              tAACGCTAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAgg                                                                                                                                                                                                                >  1:183721/1‑250 (MQ=255)
               aaCGATGACACGCTTCAAGTTCAGCATACGTTAACTTTCTGCGATAGCCGCAGATATGCCAGTAAAGAGATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTAGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTATTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGTGGAt                                                                                                                                                                                                              >  2:212867/1‑251 (MQ=255)
                       cacGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGGGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGa                                                                                                                                                                                                      <  2:401836/251‑1 (MQ=255)
                                   tCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAg                                                                                                                                                                                           <  2:537735/250‑1 (MQ=255)
                                    cAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAgcg                                                                                                                                                                                         <  1:450254/251‑1 (MQ=255)
                                                          aTAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAgg                                                                                                                                                                   <  2:183721/251‑1 (MQ=255)
                                                               agcagATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGaaa                                                                                                                                                               >  2:101709/1‑250 (MQ=255)
                                                                gcagATATGCCAGTAAAGAAAGCCCATTTGACTATTTTTTTGATAAGCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGTCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATTATCCATGGTAAGTCTGCCGCCCATATTAGCTTCGCCGAGGATTGACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGTaaaaa                                                                                                                                                             <  1:578104/251‑1 (MQ=255)
                                                                 cagATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGaaacac                                                                                                                                                            >  2:156434/1‑248 (MQ=255)
                                                                 aagATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGGTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAAc                                                                                                                                                            <  2:395446/250‑1 (MQ=255)
                                                                        gCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCaaa                                                                                                                                                      <  1:365913/250‑1 (MQ=255)
                                                                        gCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCaaa                                                                                                                                                      <  1:124076/250‑1 (MQ=255)
                                                                                     tCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGTGGGAAAAACAGCAAATTTATTCAACAAgg                                                                                                                                        >  2:538/1‑251 (MQ=255)
                                                                                        cATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCg                                                                                                                                      >  1:61214/1‑250 (MQ=255)
                                                                                          ttttccTATTTTTTAGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATACTTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTTGCGCAATCAGATATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGAt                                                                                                                                    >  2:320028/6‑250 (MQ=255)
                                                                                            tGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCAAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGCAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTCTTCAACAAGGCGAtcac                                                                                                                                 >  1:236017/1‑248 (MQ=255)
                                                                                             gACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAAcc                                                                                                                                >  2:323589/1‑251 (MQ=255)
                                                                                                     ttttGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGAt                                                                                                                                                                                              <  2:410009/181‑1 (MQ=255)
                                                                                                     ttttGATAATCTTCTTCGCTTTAGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGAt                                                                                                                                                                                              >  1:410009/1‑181 (MQ=255)
                                                                                                                      gCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTg                                                                                                       <  2:294243/251‑1 (MQ=255)
                                                                                                                          tCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGCTATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCttt                                                                                                   >  1:295362/1‑251 (MQ=255)
                                                                                                                            gAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGTGGCTTCCTCCATGTTTGCTTTa                                                                                                  >  2:95715/1‑250 (MQ=255)
                                                                                                                               caacGCGTGCGCCTTTCGAAAAGCAAGCATTATATAATGCCAGGCCATCTCTACTTCAATTGTCCCGTATTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAAGTCTGCCGCCCATATAAGCCTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATGAGAGATATAGTCTGATGGAAAAACAGCAAATTTATTCAACAAGGCGAGATCCTGCTCTTGTGCTTCCTCCATGTTTGCGTAAAAgg                                                                                              <  2:251429/251‑1 (MQ=255)
                                                                                                                                    cGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTAtt                                                                                          <  1:520991/250‑1 (MQ=255)
                                                                                                                                       gcgcCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCt                                                                                      >  1:545294/1‑251 (MQ=255)
                                                                                                                                        cgcCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTc                                                                                     >  1:69953/1‑251 (MQ=255)
                                                                                                                                               cGAGAAGCAAGCATTATATAATGCCAGGCCAGATCTTCTTCAATTGGCCCGTTTTGAAAAGCTGTGCTTGAGATCGAGATCAGCCATGATAATTCTGCCGCCCAGATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAAGTTATTCAACAAGGCGATAACCTGCTCTGGGGCCTCCGCCTGGTTCGCGTTAAAGGTATTGGCTCTATGGTc                                                                              <  2:356335/251‑1 (MQ=255)
                                                                                                                                                             tatataATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGaaaa                                                                 <  2:183218/250‑1 (MQ=255)
                                                                                                                                                                     gccaggccagTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCAt                                                         <  1:268351/250‑1 (MQ=255)
                                                                                                                                                                           ccagTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGca                                                  <  1:101709/251‑1 (MQ=255)
                                                                                                                                                                                   tcttcAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACt                                          >  2:230690/1‑251 (MQ=255)
                                                                                                                                                                                               ccGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCGGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGAGATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCTATGTTGGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTc                              <  1:271474/251‑1 (MQ=255)
                                                                                                                                                                                                    ttGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATa                          <  1:488606/250‑1 (MQ=255)
                                                                                                                                                                                                     ttgaaaGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATAc                         <  1:123657/247‑1 (MQ=255)
                                                                                                                                                                                                                           agaTCATACATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGCGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATACCCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTAAAGCCTcg  >  2:211515/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       
AAAACTGCTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTAAAGACTCG  >  NC_000913/1843908‑1844377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑