Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,346	C→T	G263R (GGA→AGA)	ydjO ←	protein YdjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,346	0	C	T	100.0%	156.2 / NA	47	G263R (GGA→AGA)	ydjO	protein YdjO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/31);  total (16/31)

GGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTT  >  NC_000913/1812101‑1812584                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               ggtggtggtGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAATCCCTTCTGGATAGCGCAGACGCCGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACCGTCTGtttt                                                                                                                                                                                                                                           >  2:188977/1‑251 (MQ=255)          gCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCATTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTGTGTATAgggg                                                                                                                                                                                                                                  <  2:376789/251‑1 (MQ=255)              cTTTCGACGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGCCGCCGAACTGGCACACCATTCATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGt                                                                                                                                                                                                                              >  2:251134/1‑251 (MQ=255)                     cgcACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCtt                                                                                                                                                                                                                        >  1:371075/1‑250 (MQ=255)                          tgatgtgcctgccggggaggggCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACttt                                                                                                                                                                                                                   <  2:476140/231‑1 (MQ=255)                          ggATTGTACGGCCTGCGGTGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACtttt                                                                                                                                                                                                                  <  2:490476/250‑1 (MQ=255)                                    gcctgcGATGGGCCTGCCAGTAACCCTGGTGGCGATGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGtt                                                                                                                                                                                                          <  2:371075/249‑1 (MQ=255)                                          gATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATcgcg                                                                                                                                                                                                  <  1:471989/251‑1 (MQ=255)                                                   gCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCAt                                                                                                                                                                                         <  1:58194/251‑1 (MQ=255)                                                    ccAGTAACCCTGGTGGCGATGTTATTCTCCGGTGAACCGCTTATCGGCAGTGGCTGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCTATAAATCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACCCCATGAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATTCATa                                                                                                                                                                                        <  1:27844/251‑1 (MQ=255)                                                                   gcgcTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTaa                                                                                                                                                                         <  1:196946/251‑1 (MQ=255)                                                                       tGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAAc                                                                                                                                                                       >  1:185539/1‑249 (MQ=255)                                                                               cccGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAg                                                                                                                                                             <  1:199106/250‑1 (MQ=255)                                                                                ccGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCa                                                                                                                                                              <  1:251134/249‑1 (MQ=255)                                                                                     gAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGaaaa                                                                                                                                                       <  2:208431/251‑1 (MQ=255)                                                                                            ttATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGGCTTTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTc                                                                                                                                                >  2:167240/1‑251 (MQ=255)                                                                                                         ccGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATa                                                                                                                                   <  1:57222/251‑1 (MQ=255)                                                                                                            tACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACtt                                                                                                                                          <  1:135254/241‑1 (MQ=255)                                                                                                            tACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGATGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGGAGACGACGCAGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCCTATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACtt                                                                                                                                          >  2:135254/1‑241 (MQ=255)                                                                                                                           tAGTGGCTCGATGACAGCTGGCCCGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGGTAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaatgac                                                                                                                 >  2:220298/1‑250 (MQ=255)                                                                                                                                   cGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCAGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAAGGTGcc                                                                                                           <  2:304597/249‑1 (MQ=255)                                                                                                                                   cGATGACAGCGGGCACGCTGACCAGCCAGTGGCGGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACTCCGAACTGGCACACCATGAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTGTAAATGTTCCATCGCGGCGATGCATACTGACTCATAATTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCt                                                                                                          <  2:495008/250‑1 (MQ=255)                                                                                                                                     aTGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGACGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCACTACATTCCTGGCTCAATGAATGTGCCTga                                                                                                        >  2:328507/1‑249 (MQ=255)                                                                                                                                        aCAGCTGGCACGTTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTGATGCTGTCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACACATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCg                                                                                                    <  1:386636/251‑1 (MQ=255)                                                                                                                                               gCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCTAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCt                                                                                             <  2:463819/251‑1 (MQ=255)                                                                                                                                                  cGCTGAC‑AGCCAGTGTCTGAAGTAAACCGATAAAGCCATTCTGGATAGCTAAGACGACGCCGAACTGGCACAACATTAATTCTTATTCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCAGCGCGGCTATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTGATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTaaa                                                                                         <  1:79972/251‑1 (MQ=255)                                                                                                                                                   gCTGACCAGCCAGTGGCTGCAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCtcaa                                                                                          >  1:151070/1‑247 (MQ=255)                                                                                                                                                      gACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAAcc                                                                                      <  1:226250/251‑1 (MQ=255)                                                                                                                                                                          aaaCCGATAAAGCCATTCTGGCTAGCGCAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCCTAGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCt                                                                  >  2:567459/1‑251 (MQ=255)                                                                                                                                                                                aTAAAGCCATTCTGGATAGCGAAGACGACTCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGGTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTAGCAATACATTCCTGGCTCAATGAATTTGCGTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGa                                                            <  1:37747/251‑1 (MQ=255)                                                                                                                                                                                aTAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTg                                                             >  2:462228/1‑250 (MQ=255)                                                                                                                                                                                   aaGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACt                                                         >  1:161400/1‑251 (MQ=255)                                                                                                                                                                                        aTTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTg                                                    >  1:433515/1‑251 (MQ=255)                                                                                                                                                                                            tGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTCCTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAAt                                                >  1:302228/1‑251 (MQ=255)                                                                                                                                                                                             ggATAGCGAAGACGACTCCGAACTGGCACACCATTAATTCTTATGCGGGCAACGGTCTGGTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGTCTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCTAGACGTTTTCTAAGTGAACTATCTGGAAt                                                <  1:354394/250‑1 (MQ=255)                                                                                                                                                                                              gATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATGCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATAc                                              <  1:261970/251‑1 (MQ=255)                                                                                                                                                                                                 aGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCa                                           <  1:4987/251‑1 (MQ=255)                                                                                                                                                                                                 aGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCa                                           <  1:188977/251‑1 (MQ=255)                                                                                                                                                                                                   cGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCat                                          >  2:183934/1‑250 (MQ=255)                                                                                                                                                                                                        acggcgCCGAACTGGCACACCATTAATTCTTATGCTGACAACGGTCTGTTTTGTATATGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCTTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATc                                    <  2:18104/247‑1 (MQ=255)                                                                                                                                                                                                         cgacgCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCt                                   <  1:462228/251‑1 (MQ=255)                                                                                                                                                                                                         cgacgCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATGCCTGGCTCAATGAATGTGCCTGTAGCTAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCt                                   <  2:568519/251‑1 (MQ=255)                                                                                                                                                                                                               cGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGc                             <  2:451547/251‑1 (MQ=255)                                                                                                                                                                                                                     ggCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCAcacca                       <  2:324789/251‑1 (MQ=255)                                                                                                                                                                                                                               tAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCAtt              <  1:216240/250‑1 (MQ=255)                                                                                                                                                                                                                               tAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCAtt              <  2:65841/250‑1 (MQ=255)                                                                                                                                                                                                                                           tGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGAttt  >  1:284308/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               GGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTT  >  NC_000913/1812101‑1812584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑