Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,795,562	C→T	D795N (GAT→AAT)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,795,562	0	C	T	98.2%	185.1 / NA	55	D795N (GAT→AAT)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (35/19);  minor base G (0/1);  total (35/20)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATCCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGC  >  NC_000913/1795322‑1795802                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 gcgcCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAatgat                                                                                                                                                                                                                                        <  2:140366/251‑1 (MQ=255)     cGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCt                                                                                                                                                                                                                                    >  1:173386/1‑251 (MQ=255)     cGTGCTGTAATGGGAATATCCTAGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCt                                                                                                                                                                                                                                    <  1:378344/251‑1 (MQ=255)           gTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAatgat                                                                                                                                                                                                                                        >  1:463144/1‑241 (MQ=255)           gTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAatgat                                                                                                                                                                                                                                        <  2:463144/241‑1 (MQ=255)                                      gggTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCAACTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCACGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGCTGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTgg                                                                                                                                                                                                   >  2:5790/1‑251 (MQ=255)                                             gTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGt                                                                                                                                                                                            >  1:18118/1‑251 (MQ=255)                                              tCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGt                                                                                                                                                                                            >  2:152933/1‑250 (MQ=255)                                                     cGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCAACTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCa                                                                                                                                                                                     >  1:527818/1‑250 (MQ=255)                                                        tGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAAtct                                                                                                                                                                                 >  1:559159/1‑251 (MQ=255)                                                            tCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTGGGCGACGGTAGCGGCAATctcct                                                                                                                                                                              <  2:336165/250‑1 (MQ=255)                                                                aTCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCtcttc                                                                                                                                                                          <  2:173386/250‑1 (MQ=255)                                                                        gATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAgtgt                                                                                                                                                                 >  1:487837/1‑251 (MQ=255)                                                                            taaGTTACCAAAACCAGAGAGTGTCACCTGTTCGCCGGTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTGGGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCGGGAATCGCTCTTTTAATGCCTCTTCACATTTGGCGACGTTAGCGGCAATCTCCTCTGCCTCGAGTGTa                                                                                                                                                                <  2:545574/247‑1 (MQ=255)                                                                            gAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCACTCTCCTCTTCTTCGCGTGTACg                                                                                                                                                              >  1:545574/1‑250 (MQ=255)                                                                                       aaCCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACGCATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTg                                                                                                                                                  <  1:395043/251‑1 (MQ=255)                                                                                           agagagTTTCAACTGTTCGCCGTTTTACAGAGCGCGACGGATCTCTTCGCAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATCGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAgg                                                                                                                                              >  2:391348/1‑251 (MQ=255)                                                                                                       cTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATg                                                                                                                                  >  1:299588/1‑251 (MQ=255)                                                                                                                gTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGctc                                                                                                                         >  1:345719/1‑251 (MQ=255)                                                                                                                 ttttCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATTCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGctct                                                                                                                        >  2:317676/1‑251 (MQ=255)                                                                                                                  tttCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCtt                                                                                                                       >  2:214693/1‑251 (MQ=255)                                                                                                                  tttCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTGCAATGCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCGCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTAGGGCGAGGctct                                                                                                                        <  2:527818/250‑1 (MQ=255)                                                                                                                      cAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGAGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATAc                                                                                                                   >  1:337891/1‑251 (MQ=255)                                                                                                                           cgcgACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTc                                                                                                              >  2:508752/1‑251 (MQ=255)                                                                                                                            gcgACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGTATCAGGCGTATGTCGAGTCTCTTATACCCCTcc                                                                                                             <  2:345719/251‑1 (MQ=255)                                                                                                                                   aTCTCTTCGAAAAGCAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAACCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCACTCTCCTCTTCTTCGCGTGTACGGCTGGTATCTTGCAGGATCAGCCTTATGGCGAGGCTCTTATCCCCCTCCGcaccac                                                                                                      >  2:171746/1‑251 (MQ=255)                                                                                                                                          cGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTAcc                                                                                               <  1:322864/251‑1 (MQ=255)                                                                                                                                          cGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTAcc                                                                                               <  1:214693/251‑1 (MQ=255)                                                                                                                                           gAAAAACAGTTCAAACAGTTCTTTGGCATCCCGCTTGCTAAGCCAAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACCCCCTTAccc                                                                                              >  2:278351/1‑250 (MQ=255)                                                                                                                                                      cAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAAGTCCTCAATGATGCCTGGAATCGCTCTGTTAATGCCTCTACACATGGGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTGGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGt                                                                                    <  2:337891/250‑1 (MQ=255)                                                                                                                                                           aGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCGTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATa                                                                              <  1:251419/251‑1 (MQ=255)                                                                                                                                                            gTTCTTTGGCATCCCGCTTGCTACGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCACATa                                                                              >  2:524037/1‑250 (MQ=255)                                                                                                                                                               cTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTACTGCCTCTACACATTTGGCTACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACTGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGtt                                                                          >  1:307430/1‑251 (MQ=255)                                                                                                                                                                 ttGGCATCCCGCTTGCTTAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGTTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCACTTATCCCCCTCCGCAACGCCCTTACCGCAGTACACGTCAAATAAGttt                                                                         <  2:330245/250‑1 (MQ=255)                                                                                                                                                                   ggCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGTTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATTCCCCTCCGCAACACCCTTACCGCGGTGCACGTCAAAGAAGTTTACg                                                                      <  2:532869/251‑1 (MQ=255)                                                                                                                                                                   ggCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACg                                                                      <  1:520249/251‑1 (MQ=255)                                                                                                                                                                      aTCCCGCTTGCTAAGCCCAAGCTGAGCAAACAGATATTCTGACATTTCAGCTTGTGTAAGCGCCATAGGTTCAATTCCTCTATGATGCCTGGAATCGCTCTTTGAATGCCTCTACACATGTGGCGTCGGTAGCGGCAATCTCCTCTTCGTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTTTGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCa                                                                   <  1:233757/251‑1 (MQ=255)                                                                                                                                                                               gCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCACCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTg                                                           <  1:317676/250‑1 (MQ=255)                                                                                                                                                                                     cccAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACg                                                    >  1:202088/1‑251 (MQ=255)                                                                                                                                                                                       cAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGCTGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTCAACGTCAAATAAGTTTACGCCAACTACCTGATTTACGcc                                                  >  2:160638/1‑251 (MQ=255)                                                                                                                                                                                           cTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACt                                              >  1:440038/1‑251 (MQ=255)                                                                                                                                                                                             tATCAAACAGATATTCTGACATTTCTGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGAAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACttt                                            >  2:576001/1‑251 (MQ=255)                                                                                                                                                                                                      gATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTc                                   >  1:311187/1‑251 (MQ=255)                                                                                                                                                                                                         atTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGGGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTAAGCAAACCACCTGATTTACGCCAACTTTATTACATTCgg                                 >  2:136101/1‑250 (MQ=255)                                                                                                                                                                                                           tCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGAt                               >  1:499897/1‑250 (MQ=255)                                                                                                                                                                                                             tGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGCTCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATaa                             >  2:127991/1‑250 (MQ=255)                                                                                                                                                                                                               aCATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATaaaa                           <  2:487837/250‑1 (MQ=255)                                                                                                                                                                                                                      aGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGTCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGCGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCt                   >  1:133834/1‑251 (MQ=255)                                                                                                                                                                                                                      aGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCt                   >  1:479186/1‑251 (MQ=255)                                                                                                                                                                                                                      aGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAAAACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACATGATTTACGCCAACTTTATTCCATTCGGATAAAATATCCgat                   >  1:100191/1‑249 (MQ=255)                                                                                                                                                                                                                              tAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTCCGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAAc           >  2:70789/1‑251 (MQ=255)                                                                                                                                                                                                                                  cgcCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGttt       <  1:199918/251‑1 (MQ=255)                                                                                                                                                                                                                                     cATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCt    >  1:435727/1‑251 (MQ=255)                                                                                                                                                                                                                                     cATAGGATCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGACGACGGTAGCGCCAATCTCCTCTTCTTCGAGTGTACAGCTGGTATCTTGCAGGATCAGGATTATGGAGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACCCGTCAAATAAGTTTACGCCAACTACCTGAATTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGACCGTTTTCt    >  1:69651/1‑251 (MQ=255)                                                                                                                                                                                                                                       tAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGTTAGAGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCCACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGc  >  2:33503/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 GCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATCCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGC  >  NC_000913/1795322‑1795802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑