Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,791,057	C→T	G63D (GGT→GAT)	selO ←	protein adenylyltransferase SelO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,791,057	0	C	T	97.7%	137.4 / ‑6.5	44	G63D (GGT→GAT)	selO	protein adenylyltransferase SelO
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (18/24);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGA  >  NC_000913/1790816‑1791302                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      cgGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTa                                                                                                                                                                                                                                              <  2:93457/251‑1 (MQ=255)           aGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAAGAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTcgcc                                                                                                                                                                                                                                    <  2:458557/251‑1 (MQ=255)                                gCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCAtt                                                                                                                                                                                                               >  2:228147/1‑251 (MQ=255)                                    gaCTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAAAAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAACAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGAAGGGCTTCGCCCCCCCAGCCACCTGCGCCAttttt                                                                                                                                                                                                            >  1:41931/1‑250 (MQ=255)                                         tcgcgtttctacgAACGTAAAACCGCCCCGCCATCACCCGTTCGCGAATAAGGCGTCAGGCCAGCTCCTTTCAGATGCCAGTCCATTGTAGCGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCGTCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGGAAACCGGCGCCAGTGGTGACATATCATGCTGTAGGGCGTCTCCGCCCCAGACACCTGCGTCATTTTTAAACa                                                                                                                                                                                                       <  2:354802/239‑1 (MQ=255)                                                     gAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAAt                                                                                                                                                                                          >  1:470694/1‑251 (MQ=255)                                                          aaaaaCCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTATCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACGCCTGCGCCATTTTTAAACAGCGACGATGGAATACTCa                                                                                                                                                                                     <  2:90309/250‑1 (MQ=255)                                                           aaaaCCGCCCGTCCATCCCCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCa                                                                                                                                                                                     <  2:298434/250‑1 (MQ=255)                                                           aaaaCCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCa                                                                                                                                                                                     <  1:112384/250‑1 (MQ=255)                                                                    cGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCAATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGc                                                                                                                                                                           <  1:171069/251‑1 (MQ=255)                                                                         aTCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGtt                                                                                                                                                                      >  1:203155/1‑251 (MQ=255)                                                                           cACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCg                                                                                                                                                                    >  1:225038/1‑251 (MQ=255)                                                                              ccATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTa                                                                                                                                                                             <  2:555777/239‑1 (MQ=255)                                                                              ccATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTa                                                                                                                                                                             >  1:555777/1‑239 (MQ=255)                                                                               cATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGtat                                                                                                                                                                >  1:313684/1‑251 (MQ=255)                                                                                          aaGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGGAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGGTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCGGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGGGTTAGCCAGTGCGGTATTATGCCAAATc                                                                                                                                                     <  2:48494/251‑1 (MQ=255)                                                                                            ggCGTCAGGACAGCACCGTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTAGGCCAAATCAg                                                                                                                                                   <  2:188971/251‑1 (MQ=255)                                                                                                         cACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTAttt                                                                                                                                      <  2:313684/251‑1 (MQ=255)                                                                                                          aCCTTTCAGATGCCAGTCCATTGGAGTGTCAGCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTTACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACTCCTGCGCCATTTTTAAACAGCGACGATGGAATTCTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTa                                                                                                                                     <  1:340612/251‑1 (MQ=255)                                                                                                                           ccATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGt                                                                                                                    <  2:199884/251‑1 (MQ=255)                                                                                                                            cATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTg                                                                                                                   <  1:228147/251‑1 (MQ=255)                                                                                                                                ggaGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTg                                                                                                                <  1:484030/248‑1 (MQ=255)                                                                                                                                gTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTGGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGt                                                                                                               <  1:424307/251‑1 (MQ=255)                                                                                                                                           agcaagcaGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTg                                                                                                    >  2:354924/1‑251 (MQ=255)                                                                                                                                                         cGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTGTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGGTCGGTATTATGCCAAATCAGCCGGGCATTATGTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCgcgc                                                                                      <  1:31677/251‑1 (MQ=255)                                                                                                                                                                tAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCTGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGAAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCggg                                                                               >  1:543826/1‑251 (MQ=255)                                                                                                                                                                   aaTGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTa                                                                             >  1:217279/1‑250 (MQ=255)                                                                                                                                                                      gCCGCGCCCATCACCCCGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACa                                                                          >  1:505793/1‑250 (MQ=255)                                                                                                                                                                        cgcgCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACaaa                                                                        >  2:585127/1‑250 (MQ=255)                                                                                                                                                                            cccATCACACAGTTCGCACGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGCGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAGGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGAAACAAACGAc                                                                    >  2:377876/1‑250 (MQ=255)                                                                                                                                                                               aTCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTGTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAggg                                                                <  1:217237/251‑1 (MQ=255)                                                                                                                                                                                 ccccctgcttgcccgcccAGACGCCGAACGGATGCGCACTGTAAACCTGCGCCAGGGGTGACATATCAGGCAGAAGGGCTTCGCCGCCCCAGACACCTGCGCCAGTTTTAAACAGCGACGATGGAATACTCAGCGGGTTAGCCAGTGCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGTTTTCTGGCAATTCATCGCGCTAGTGGGTGACAAAAGACAGGGt                                                               <  2:390466/240‑1 (MQ=255)                                                                                                                                                                                 cACCCAGTTGGCCCGCCCAGACGCGTAACTGCTGGTCACTGGAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCGTCGCCGCCCCAGACACCTGCGCCATGTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCATGCGGGCATTATTTAAAGGCGGAGGGGAAAGTGCTGTAGAGGGTTCTGGCAATTCATCGCGGCAGCTGGTAACAAAAGGCAGGGTc                                                              <  2:41931/251‑1 (MQ=255)                                                                                                                                                                                    ccAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATa                                                           <  1:354924/251‑1 (MQ=255)                                                                                                                                                                                               gcccAGACGCCGAACTGATGACCACTGTAACCCTGCGCCCGCGGTGACATATCAGTCAGTAGAGCTTCGCCGCCCCAGACACTTACGCCATTTTTACACATCGACGCTGTCATCCTCAGCGAGTTAGCCAGTTCGGTATTCAGCCAAATCAGCAGGGCATTATTTACAGGCGTCGGGGAAAGAGCTGTATAGGTTTCTGGCATTTCATCGCTCCTGCGGGTAACAACAGTCAgggcccttaatccaccttt                                                >  2:312785/1‑235 (MQ=255)                                                                                                                                                                                                            aCTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGa                                   <  2:470694/251‑1 (MQ=255)                                                                                                                                                                                                                         gTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACtctc                        <  2:402591/249‑1 (MQ=255)                                                                                                                                                                                                                          tAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTc                     >  1:206491/1‑251 (MQ=255)                                                                                                                                                                                                                           aaaCCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGAGAGTGTAGACGGTTACTCTCGTCt                    <  2:472692/251‑1 (MQ=255)                                                                                                                                                                                                                                   gcCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGCTCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACAcc            >  2:176433/1‑251 (MQ=255)                                                                                                                                                                                                                                   gcCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACAcc            >  1:334823/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggtgACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCGGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCaa       >  2:510616/1‑250 (MQ=255)                                                                                                                                                                                                                                         ggtgACATAACAGGCAGTAGGGCTTTGCCGCCCCAGACTCCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTAGAGGTTTCTGGCAATTCAGCGCTCCAGCGGGTAACAAAAGACAGGGGCATAGATCCTCCGGTTTGAGAGTGTAGACGGTTACTCTCGTCTTAAACACGAGCaa       <  2:574238/250‑1 (MQ=255)                                                                                                                                                                                                                                              cATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGa  >  1:390506/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGA  >  NC_000913/1790816‑1791302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑