Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,786,965 C→T A50T (GCC→ACC)  ppsA ← phosphoenolpyruvate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,786,9650CT92.9% 71.0 / ‑6.1 29A50T (GCC→ACC) ppsAphosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (6/20);  minor base G (2/0);  total (9/20)
Fisher's exact test for biased strand distribution p-value = 7.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAGGAGCGCACCGCAAAAGAGGCGTTTTCGTCATCGGCGGAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAATCGTTCA  >  NC_000913/1786725‑1787211
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       
cGGAGGAGCGCACCGCAAAAGAGGCGTTTTCGTCATCGGCGGAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTc                                                                                                                                                                                                                                               <  1:133703/251‑1 (MQ=255)
    ggagCGCACCGCAAAAGAGGCGTTTTCGTCATCGGCGGAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTcggaa                                                                                                                                                                                                                                           <  1:383332/251‑1 (MQ=255)
      agCGCACCGCAAAAGAGGAGTGTTCGTCAGCGGCGGAACGCTGTGCAGAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACGGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGTCGCGTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTcggaacg                                                                                                                                                                                                                                         <  2:93769/251‑1 (MQ=255)
        cgcACCGCAAAAGAGGCGTTTTCGTCATCGGCGGAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGTAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTcggaacgga                                                                                                                                                                                                                                       <  2:62456/251‑1 (MQ=255)
         gcACCGCAAAAGAGGCGTTTTCGTCATCGGCGGAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTcggaacggaa                                                                                                                                                                                                                                      <  1:125740/251‑1 (MQ=255)
           aCCGCAAAAGAGGCGTTTTCGTCATCGGCGGAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGCTTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCCTAACTGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGGTGTGGTGGAACCaattggagagggaa                                                                                                                                                                                                                                     >  2:199866/1‑237 (MQ=255)
                   agagGCGTTTGCGTCATCGGCGGAAAGCTGTGCAGAGGCTGCGCGGATGGCGTGTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTGTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCGTTCGGAACGGAAACACCCA‑TTc                                                                                                                                                                                                                           <  2:540286/251‑1 (MQ=255)
                        cGTTTTCGTCATCGGCGGAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGaa                                                                                                                                                                                                                      <  1:79117/251‑1 (MQ=255)
                                      gcggAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAAACATTCGGAACGGAAAAACCCC‑TTCCGGAAAAATTAGTAATCAt                                                                                                                                                                                                        >  1:304759/1‑251 (MQ=255)
                                                gtgCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAgg                                                                                                                                                                                              <  1:554170/251‑1 (MQ=255)
                                                  gCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGGGAAACCCTTCGGAACGGGAACACCCC‑TTCCGGAAAGATTAGTAATCATTTCACCCAgggg                                                                                                                                                                                            >  1:99329/1‑250 (MQ=255)
                                                    aTAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAgg                                                                                                                                                                                          <  2:121397/251‑1 (MQ=255)
                                                            cgctgATGGCGTTTTCCAGCTCAGGCTGGTAGGGAGTGTCGATAATTCACTGGCGGATTTGCGCGCCCGCGTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGTAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCAttttt                                                                                                                                                                                   <  1:307190/246‑1 (MQ=255)
                                                                                    gCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATTCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCAGTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGGAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACAtcattc                                                                                                                                                          <  1:561227/251‑1 (MQ=255)
                                                                                             gAGTGTCGACAATCCACCAGCCGATTTGCGCGCCCGCTCTCGCAAGCTAAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGTTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGTACGGCGACACCCACTTCCG‑AAAGATTAGGAATCATTTTACCCAGGGAGGCCCTTTTTCCCCCAACCCTGTCTACATCCTTCCTGCCgggt                                                                                                                                                 >  2:406696/1‑248 (MQ=255)
                                                                                                          ccACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGGGAAACCCATCGGAACGGAAACACCCA‑ATCCCGAAAGATTAGTAATCATTTCACCCCGGGAGGCATTTTTTCCCCCCACCCTGTCTACATCATTCATGCCGGGTTGGTTATACCaa                                                                                                                                     >  1:386517/1‑250 (MQ=255)
                                                                                                                 cGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAg                                                                                                                             <  1:292491/251‑1 (MQ=255)
                                                                                                                        gcgcgcCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGa                                                                                                                       <  1:298221/250‑1 (MQ=255)
                                                                                                                               cccTTTCGCAAGCTGAGTAACATCGTCATTATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGTGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACCCCCA‑TTCCGGAAAGATTAGTACTCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTTACGAGCCAt                                                                                                               <  2:118088/249‑1 (MQ=255)
                                                                                                                                gCTTTCGCAAGCTGAGTAACATCGTCAATTTCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCTCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAACCCATTCGGAACGGAAACCCCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCACCCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGGGACGAGCCAtt                                                                                                              <  2:355864/251‑1 (MQ=255)
                                                                                                                                     cGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTgg                                                                                                         <  1:358529/251‑1 (MQ=255)
                                                                                                                                                                                                       gcTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACCCCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTg                                       <  2:118144/251‑1 (MQ=255)
                                                                                                                                                                                                       gcTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCtt                                                                                         <  1:572886/201‑1 (MQ=255)
                                                                                                                                                                                                         tttGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTATGGTGAAAACATTAGGAACGGAAACACCCA‑ATCCGGAAAGATTAGAAATCATTTCAACCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCCCCAGCGGTGACGAGCCACTGTTGGACATCGAACAATCCTTTAGTGCTAAATGAACGGGTTGAGAAACACATTTATGCGCATTTATCTTTGcc                                     >  1:145460/1‑250 (MQ=255)
                                                                                                                                                                                                                  gAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGGAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTTCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATTTTTGCATATTTAAcc                            >  2:86314/1‑251 (MQ=255)
                                                                                                                                                                                                                  gAAACTGGTTAAACGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGCAACACATTTCTGCGCATTTATCTTTGCATATTTAAcc                            >  1:123208/1‑251 (MQ=255)
                                                                                                                                                                                                                      cTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAAAACCCA‑TTTCGGCAAGATTTGTAAACATTTCACCCAGGGAGGCATTTTTGCCCCCCCCCCTGTCTACATCATTCATGCCGGGTTGGTTATAACAAAGCACCAGCGGTGACGAGCCCTTTGTGGACATCGAACAATCCTTTTTTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTTTCTTTTCATATTTTACCgggt                        >  2:80497/1‑249 (MQ=255)
                                                                                                                                                                                                                            aaaCGCGTCGGCGGTTGTGGTGAAACCATTCGGAACGGAAACACCCT‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGTCATTTTTGCCCCCATCCCTGGCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGaaaaa                  <  2:145460/251‑1 (MQ=255)
                                                                                                                                                                                                                                           tgtgGTGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAATCGtt    <  1:199866/250‑1 (MQ=255)
                                                                                                                                                                                                                                            gtggtgAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCCCCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATAGTTAACCGGATGAAAAAAACGGTGAATCGtcca  <  1:354368/251‑4 (MQ=255)
                                                                                                                                                                                                                                            gtggtgAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAATCGTTc   >  1:310691/1‑250 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       
CGGAGGAGCGCACCGCAAAAGAGGCGTTTTCGTCATCGGCGGAAAGCTGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCA‑TTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAATCGTTCA  >  NC_000913/1786725‑1787211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑