Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,743,164	C→T	E27K (GAG→AAG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,743,164	0	C	T	100.0%	136.0 / NA	41	E27K (GAG→AAG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/23);  total (18/23)

TCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCC  >  NC_000913/1742930‑1743405                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  tCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACg                                                                                                                                                                                                                                    >  1:96895/1‑250 (MQ=255)       tatGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAAttt                                                                                                                                                                                                                             >  1:260688/1‑251 (MQ=255)                  aTTAAGGGTCCGCCAATTTCATCACTTAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGta                                                                                                                                                                                                                  <  2:129715/251‑2 (MQ=255)                                       tcaCTGCATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGt                                                                                                                                                                                             >  2:54815/1‑251 (MQ=255)                                       tcaCTGACTTTCGAAGAACGCTCAACGTTTAACCAATCCCCCACTTTAAAATCGCCAAGCTTCGTAATGCGTAACGCTTCTTTCATCAGGTAAAAACTGCCATGGTTGCCGTTAATTTCCGTCACGATCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAGTCGagataggacgagcgtc                                                                                                                                                                                             >  2:375492/1‑236 (MQ=255)                                       tcaCTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTGCATCAGGTCAAAACTGACATGGTTTCCGTTAATTTCCGTCTCGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCg                                                                                                                                                                                                             <  1:375492/235‑1 (MQ=255)                                             aaTTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCTTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTg                                                                                                                                                                                       <  1:43111/251‑1 (MQ=255)                                                 tCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAACCTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATCTGGTCGGGTAACTtcacc                                                                                                                                                                                                                                               >  1:480826/1‑191 (MQ=255)                                                   gccgcACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGGTAATTTCCGTCACGGGCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGGGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAAt                                                                                                                                                                                 <  1:221205/251‑1 (MQ=255)                                                             cAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAAc                                                                                                                                                                       >  1:498468/1‑251 (MQ=255)                                                             cAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTACGACACGGTCCGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATAGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAAc                                                                                                                                                                       >  1:584355/1‑251 (MQ=255)                                                                cGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACAt                                                                                                                                                                     >  2:164557/1‑250 (MQ=255)                                                                    tACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCATTCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACtt                                                                                                                                                                 >  1:176970/1‑250 (MQ=255)                                                                       ccAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACaa                                                                                                                                                             <  1:580846/251‑1 (MQ=255)                                                                        cAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACAt                                                                                                                                                                     >  1:98560/1‑242 (MQ=255)                                                                        cAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAAc                                                                                                                                                            <  2:166742/251‑1 (MQ=255)                                                                        cAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTGGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACAt                                                                                                                                                                     <  2:98560/242‑1 (MQ=255)                                                                                   ttttAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGCAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGAGCCCTGTACAATCCCCGTAAACATAATTACAAATCCTGAAATc                                                                                                                                                  >  2:493967/1‑250 (MQ=255)                                                                                            gCCAAGATTGGGAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATGTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTGCGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACa                                                                                                                                        <  2:121368/251‑1 (MQ=255)                                                                                                aGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCt                                                                                                                                    <  2:96895/251‑1 (MQ=255)                                                                                                      gTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAg                                                                                                                              <  1:21103/251‑1 (MQ=255)                                                                                                          tGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGcac                                                                                                                           >  1:486812/1‑250 (MQ=255)                                                                                                          tGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGcac                                                                                                                           >  2:445590/1‑250 (MQ=255)                                                                                                                    ttctttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGt                                                                                                                 <  1:54815/250‑1 (MQ=255)                                                                                                                         tcatcaGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGaaa                                                                                                            >  1:153082/1‑250 (MQ=255)                                                                                                                           atcaGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACg                                                                                                         >  1:393428/1‑251 (MQ=255)                                                                                                                                  cAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACa                                                                                                                                                                                   >  1:534102/1‑170 (MQ=255)                                                                                                                                  cAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACa                                                                                                                                                                                   <  2:534102/170‑1 (MQ=255)                                                                                                                                   aaaaCTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTAcc                                                                                                 <  1:505267/251‑1 (MQ=255)                                                                                                                                    aaaCTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTAcc                                                                                                 >  1:389432/1‑250 (MQ=255)                                                                                                                                                   ccGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGaa                                                                                 <  2:112466/251‑1 (MQ=255)                                                                                                                                                         aTTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTa                                                                           <  1:164557/251‑1 (MQ=255)                                                                                                                                                             ccGTCACGGTCAGGCAGCAACCGTTATGCTCCACGGAAGCACCTGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATGTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACGTACAGCTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAGTAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTAttt                                                                        <  1:256178/250‑1 (MQ=255)                                                                                                                                                                         ggCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGaa                                                           <  2:153082/251‑1 (MQ=255)                                                                                                                                                                           cagcaACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAAtt                                                         >  2:285424/1‑251 (MQ=255)                                                                                                                                                                                           ccACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTAc                                         <  2:486812/251‑1 (MQ=255)                                                                                                                                                                                                  aGCACCGGTTTCCAGTCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCTATTACCAGTGAAGGAGTAAGAATGGCGATTTTTTCACTTGAGAATTAATAAATCCGCGCTACAATAgcc                                  <  2:399412/251‑3 (MQ=255)                                                                                                                                                                                                    cACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCCCTGGAGAATTAATAAATCCTCGCTACAATAGACTg                                <  2:319143/251‑1 (MQ=255)                                                                                                                                                                                                             ccAGGCCGTCCAGCATGTGGTCGGGTAACTTCCCCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACGGGAGAATGAATAAATCCTCGCTACAATAGACTGAATTTcccc                       <  2:393428/251‑1 (MQ=255)                                                                                                                                                                                                                           atggggTCGGGTAACTTCACCACATGCATATGTAAATTTGGTTTCTCGGCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACAGTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTGTTTCACTGGTGAATTAATAAATCCTCGCTAAAATAGACTGAATTTCCCATGCTTCTTCtgttt         <  2:467065/247‑1 (MQ=255)                                                                                                                                                                                                                            tgtgGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTATCGTCAATCGACACCAGTTTTGCGATGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGATGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCCCCTGCTTCTTCtttttt        >  2:347657/1‑250 (MQ=255)                                                                                                                                                                                                                                  cGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGccc  <  2:389432/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  TCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCC  >  NC_000913/1742930‑1743405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑