Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,631	T→C	L263P (CTG→CCG)	purR →	DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,631	0	T	C	100.0%	182.5 / NA	61	L263P (CTG→CCG)	purR	DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/35);  total (26/35)

CCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGTCGTTAATCACCCG  >  NC_000913/1738396‑1738876                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      cccGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGa                                                                                                                                                                                                                                        <  2:523741/251‑1 (MQ=255) cccGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCAGGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGa                                                                                                                                                                                                                                        <  2:240056/251‑1 (MQ=255)   cGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATg                                                                                                                                                                                                                                      <  1:445177/251‑1 (MQ=255)     gCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTAGGAAGGCGATGGAAGAAGCGATGAGCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCTATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGtt                                                                                                                                                                                                                                    <  2:464355/251‑1 (MQ=255)       ccGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGTTCGCGCGCGTCTCGCAGGATGTTTc                                                                                                                                                                                                                                  <  2:103676/251‑1 (MQ=255)            ggAACGTAACGCCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGGGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCAGCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCt                                                                                                                                                                                                                               <  2:236989/249‑1 (MQ=255)                   aaCACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGtt                                                                                                                                                                                                                       >  1:554266/1‑250 (MQ=255)                    acacCGGCGCAGGCCGCCTTGCCGGTTTTACGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCCTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTAt                                                                                                                                                                                                                     <  1:498202/251‑1 (MQ=255)                       ccGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGAt                                                                                                                                                                                                                  <  2:7800/251‑1 (MQ=255)                              aGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTgc                                                                                                                                                                                                           <  2:441821/251‑1 (MQ=255)                              aGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGAGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGCTGAAATGGGCCCGCGCGTCCCGCCGGATGTTTCGCTGATCGGTTATGATAACGTgc                                                                                                                                                                                                           >  2:322267/1‑251 (MQ=255)                                gccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTgcgc                                                                                                                                                                                                         >  2:200294/1‑251 (MQ=255)                                                            ggAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTg                                                                                                                                                                              >  1:35184/1‑250 (MQ=255)                                                             gaagaaGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGAc                                                                                                                                                                            >  1:481388/1‑251 (MQ=255)                                                                gaagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCGGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCAc                                                                                                                                                                         <  1:219534/251‑1 (MQ=255)                                                                 aagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGGGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACg                                                                                                                                                                        <  2:571700/251‑1 (MQ=255)                                                                     gatgatCACGGTGCCGGAAAGCTGGATTGTGCCGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCCTGCCGCAAATCCTGTCGCAGCCGAATCGCCCTACTGCCGTCTTCTGTGGTGGCGCTATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATACCGTGCGCAACGCGCGCTCTTTTACGCCGGCGCTGACCACGATcc                                                                                                                                                                    >  2:228104/1‑251 (MQ=255)                                                                               gTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCaa                                                                                                                                                           >  1:26458/1‑250 (MQ=255)                                                                               gTGCCGGAAAGCGGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCAGGGCAATGGGCGCACGTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTTACCACGATCCATCAGCCaaa                                                                                                                                                          <  2:42434/251‑1 (MQ=255)                                                                                  ccGGAAAGCTGGATTGTGCAGGGTTCCTTTGACCCTGAAGCCGGTTATCGCGCCCTCCAGCAGATCCTGTCGCAGCCGCACCGCCCTACTGCAGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGTCCACGATCCATCAGCCAAAAGa                                                                                                                                                       <  2:309777/251‑1 (MQ=255)                                                                                     gAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTc                                                                                                                                                    >  2:196097/1‑251 (MQ=255)                                                                                                tgtgCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCaaa                                                                                                                                                          <  1:530818/234‑1 (MQ=255)                                                                                                tgtgCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCaaa                                                                                                                                                          >  2:530818/1‑234 (MQ=255)                                                                                                      gggTGACTTTGATCCTGAATCCGTTTACCGCTGCTGGCGGCGAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTTTGGTGGCGATATCATGGCAATGGGCGCATATTGTGCTGCTGATGAAATTGGCCCGCGCGTCCCGCCGGATGTTTCGCTTATCGGTTATGATTACTTGCGCAACGCGGGCTATTTTACGCCGTCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGt                                                                                                                                   <  2:432576/251‑1 (MQ=255)                                                                                                      gggTGACTTTGAACCTGAATCCGGTTATCGCGCCAGGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCGGTGGTGGCGAGATCAGGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGt                                                                                                                                   <  2:432580/251‑1 (MQ=255)                                                                                                      gggTGACTTTGAACCTGAATCCGGTGATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTTGGTGAAACAGCGt                                                                                                                                   <  2:389012/251‑1 (MQ=255)                                                                                                         tGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTc                                                                                                                                 >  2:222791/1‑250 (MQ=255)                                                                                                          gacgttGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCaa                                                                                                                               <  1:77309/247‑1 (MQ=255)                                                                                                              ttGAACATGACTCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAACTGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTCTTTTACGCCGGCGCTGACCACGATCCATCGTCCAAACGATTCGCTGGGTGAAACAGCGTTCAACATg                                                                                                                           >  2:135416/1‑251 (MQ=255)                                                                                                                 aaCCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTg                                                                                                                        <  1:30514/251‑1 (MQ=255)                                                                                                                   ccTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGtt                                                                                                                      >  1:47556/1‑251 (MQ=255)                                                                                                                            ggTTATCGCGCCATGCAGCAAATCCGGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGatcgtat                                                                                                             <  1:343776/251‑1 (MQ=255)                                                                                                                               tATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACATTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACCGCGTTCAACATGCTGTTGGatcgtatagt                                                                                                          >  1:340673/1‑248 (MQ=255)                                                                                                                                aTCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGTGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTc                                                                                                         <  2:214216/251‑1 (MQ=255)                                                                                                                                aTCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGatcgtatcgt                                                                                                          <  1:200294/250‑1 (MQ=255)                                                                                                                                   gcgcCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTcaac                                                                                                      >  1:387596/1‑251 (MQ=255)                                                                                                                                     gcCATGCAGCAAAGCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTTGTGGCGATATCATGGCAATGGGCGCACTTTGTGCGTCTGATGAAATGGGCCCGCGCGTCCCGCAGGGTGTTTCGCTGATCGGTTATGATAACGGGCGCTACGCGCGGTATGTTGCGCCGGCGCTGAGCACGATCTATCAGCTAAAAGATTCGCTTGGGGAAACAGCGTTCGACAAGCTGGTGGATCGTAGCGTcaacaa                                                                                                    <  2:571449/251‑1 (MQ=255)                                                                                                                                         tGCAGCCAATCCTGCCACCGCCGCATCGCCCTAATGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCACGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGt                                                                                                >  2:267489/1‑251 (MQ=255)                                                                                                                                          gcagcaAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTg                                                                                               >  1:517862/1‑251 (MQ=255)                                                                                                                                           cagcaAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTg                                                                                               >  2:138604/1‑250 (MQ=255)                                                                                                                                                  tCCTGTCGCAGCCGAATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAACGATTCTCTGGGTGAAACAGCGTTCAACATGCTGTTGGATAGTATCGTCAACAAACGTGAAGAACCg                                                                                       >  2:461458/1‑251 (MQ=255)                                                                                                                                                         gCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGATGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCt                                                                                 >  1:211641/1‑250 (MQ=255)                                                                                                                                                          cAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTACCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGACGGCGCTGACCACGATCAATCAGCGAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGATGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTAt                                                                               >  1:286462/1‑251 (MQ=255)                                                                                                                                                           aGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTAtt                                                                              <  1:330200/251‑1 (MQ=255)                                                                                                                                                           aGCCGCATCGCCCTACTGCCGTCTGCTGTGGTGGCGATAGCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTAtt                                                                              <  2:362845/251‑1 (MQ=255)                                                                                                                                                           aGCCGCATCGCCCTACTGCCGTCTGCTGTGGTGGCGATAGCAGGGCAATGGGCGCACTTTGTGCGGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGGTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATGCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATTGTCAACAAACGTGGAGAACCGCAgaggata                                                                              <  2:224002/251‑7 (MQ=255)                                                                                                                                                                   cGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGTTCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGc                                                                       <  1:26564/250‑1 (MQ=255)                                                                                                                                                                   cGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGc                                                                       >  1:100993/1‑250 (MQ=255)                                                                                                                                                                       cTACTGCCGTCTTCTGTGGTGGCTATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCcg                                                                  >  1:325865/1‑251 (MQ=255)                                                                                                                                                                          cTGCCGTCTTATGTGGTGGCGATATCATGGCACTGGGCGCACTTTGTGCTGCTGATGACATGGACCCGCGCGTCCCGACGGATGTTTAGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGCTACATCAGCCAAACAATTCGCTGGGTGAAACAGCGTGCAACATGCTGTTGGATCGTATCGTCAACCACAGTGAAGAACCGCAGTCTATTGAAGTGCAACCgcgc                                                               >  2:559635/1‑251 (MQ=255)                                                                                                                                                                             ccGTCTTCTGTTGTGGCGATATCATGGCAATGGTCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCttg                                                            <  1:322267/251‑1 (MQ=255)                                                                                                                                                                                      gtggtgGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGaa                                                      <  2:131094/248‑1 (MQ=255)                                                                                                                                                                                              gATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGTTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAATCTAGTGAAGTGCATCCGCGCGTGATTTAACGCCGCTCCGt                                           <  2:334139/251‑1 (MQ=255)                                                                                                                                                                                                            gggCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGt                             >  1:106355/1‑251 (MQ=255)                                                                                                                                                                                                             ggCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGtt                            >  1:128281/1‑251 (MQ=255)                                                                                                                                                                                                              tcgcACTTTGTGCTGCTGATGAAATGGGACCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACCTGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGGGGCTGCCGGCCCGTTc                           >  2:59284/2‑251 (MQ=255)                                                                                                                                                                                                                       gtgCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTa                   <  1:196097/250‑1 (MQ=255)                                                                                                                                                                                                                        ggcttctgATGAAATGGGCCCGCGCGTCCCGCAGGATGGTTCGCTGAGCGGTTATGATAACGTGCGCAACGCGCGCTATTTTGCGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTGCCGCGACTAtc                 <  2:409560/246‑1 (MQ=255)                                                                                                                                                                                                                                    aaTGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATGTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGTCGTTAATCAc     <  1:581133/251‑1 (MQ=255)                                                                                                                                                                                                                                       gggCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCGGACCACTATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGTCGTTAATCACCCg  <  2:517862/251‑1 (MQ=255)                                                                                                                                                                                                                                       gggCCCGCGCGTCCCGCAGGATGTTGCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGTCGTTAATCAccc   <  2:482813/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      CCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGTCGTTAATCACCCG  >  NC_000913/1738396‑1738876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑