Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	83.8 / NA	25	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/9);  total (16/9)

GTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652092‑1652575                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      gtgtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGACAGCAATCGACGCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTTATGTTCACCTCCGAGAGTAt                                                                                                                                                                                                                                            >  1:406901/1‑251 (MQ=255)         gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcaga                                                                                                                                                                                                                                      <  1:418448/249‑1 (MQ=255)               actggatgtCGCCGTGGTGAGGTGTGTTCTCGTGGATGGGAGGACTTAGATATGAAAAATGGAAGATGGATGATCAGGAGAAATTTAGCAAAAGACCGTTGCACTGTACCAAATACACAGGCTGGATCCGATCGTGTCATTCACGTTAGTAAGCCACGAATCGACGCTCGCCGGAGTCAGAGGACATTATCGATACTGAGGTAAGAGCATATCATTGATGTTCACCTCAGAGAGCAGGGCAGAAGAGaaa                                                                                                                                                                                                                               <  2:580734/242‑1 (MQ=255)                                                    gggAGGACATAAATCTGAAAAATAGAACAATGATGCTCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGACCAAATAGGGTCCTTCATCTTCTTAAACCAGCAATCGACACTCCCCGGAGTCCGATGACATTAAAAAGACTGAGCAAAGAGCATATCATTGCTGTTTAACTCAGAGAGTATGGCAGAAAAGAAAAACAAAAATGCACCTTTTATTTTCAACCTTAAGTGTc                                                                                                                                                                                          >  2:391206/1‑250 (MQ=255)                                                                        aaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATTCCTCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCCAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAACGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGGGTCAGCGGGAGTAAAAAATTAT‑gg                                                                                                                                                                    >  2:542627/1‑251 (MQ=255)                                                                                  tgatgaTCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACCTTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGGGAGGAAAAAAATAT‑GGTGACCAtttt                                                                                                                                                          >  2:59537/1‑251 (MQ=255)                                                                                     tgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAAAGGGTCATTCATCTTATTCAACCAGCAATCGAAGCTCTCCGGAGTCAGATGACATTAAAGAGACCGAGCAAAGAGCATATCATTGATGTTCAACTCAGAGAGTATGGCAGAAACGAAAAAAAAAAATGCACCTTTGTTTTTCAACCTTGAGTGTCAACAAGTGTAAAAAATTAT‑gtgtaccctttttcc                                                                                                                                                       >  2:307945/1‑237 (MQ=255)                                                                                                 aTTTAGCAATAGTCCGTTTCACGGGAACAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTAGTAAGCCAGCAATCGACGCTCTCCGGTGTCAGATGACATTAACGCGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCCCCTTTGTTTTTCAACCTGAAGTGGCAGCGAGAGTAAAAAATTAT‑GGTGATCATTTTTCCGTTGAcgaaata                                                                                                                                           <  2:396723/251‑7 (MQ=255)                                                                                                           aGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCa                                                                                                                                      <  1:420518/246‑1 (MQ=255)                                                                                                           aGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAACAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGGAAAAAATTATTGGTG‑CCATTTTACCGTTGACTCAATAAGGCa                                                                                                                                      >  2:420518/1‑246 (MQ=255)                                                                                                                                                   cGGGTCCTTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAATGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGGCATCgca                                                                                          >  2:89523/1‑248 (MQ=255)                                                                                                                                                                          aGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACtt                                                                  >  1:84445/1‑251 (MQ=255)                                                                                                                                                                            cAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTAt                                                                <  2:81576/251‑1 (MQ=255)                                                                                                                                                                                cGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGcc                                                             <  2:391551/250‑1 (MQ=255)                                                                                                                                                                                 gACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGcc                                                                                                >  2:244124/1‑214 (MQ=255)                                                                                                                                                                                 gACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGcc                                                                                                <  1:244124/214‑1 (MQ=255)                                                                                                                                                                                    gCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCAAGAGAAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgctg                                                        >  1:330250/1‑251 (MQ=255)                                                                                                                                                                                          cGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCt                                                  <  1:59537/251‑1 (MQ=255)                                                                                                                                                                                          cGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCt                                                  <  1:542627/251‑1 (MQ=255)                                                                                                                                                                                                     gACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTg                                       >  1:362265/1‑251 (MQ=255)                                                                                                                                                                                                                       taaaGAGCATATCATTGATGGTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAACATGCACCTTTGTTTTTCAACCTGCAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGAACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat                       >  2:138585/2‑249 (MQ=255)                                                                                                                                                                                                                       cAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTTT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat                       >  1:369866/1‑249 (MQ=255)                                                                                                                                                                                                                          agagCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGCCTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCa                   >  1:391853/1‑250 (MQ=255)                                                                                                                                                                                                                          agagCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCa                   >  2:4845/1‑250 (MQ=255)                                                                                                                                                                                                                                          ggtCACCTCAGAGAGTATGGCAGAACAGAAAAACAATCAGGCACATTTGGTTTTCAACCTGCAGTGTCAGCGAGAGCAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGGAGGACGCAGCGATAACACTTGCCGGACTCCGCCATCAAAAATCATATCCGTCGAGACATACTGATTCCTGCTGGTCGCTGACAGCTGGTGCTAACACGGCTTTTATAGCAAACCAGATggtcactgc  >  2:119799/3‑244 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      GTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652092‑1652575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑