Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,534,321	C→T	P100S (CCA→TCA)	nhoA →	arylamine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,534,321	0	C	T	97.0%	101.6 / NA	33	P100S (CCA→TCA)	nhoA	arylamine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (13/19);  minor base G (0/1);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CGTGCATTGCACCTGAAACACAATTGCACCATTCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGCCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAGTCGATGTACCGTTTTGATCTCTGCGAGCA  >  NC_000913/1534099‑1534559                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               cGTGCATTGCACCTGAAACACAATTGCACCATTCCGATTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGttt                                                                                                                                                                                                                    <  1:560871/251‑1 (MQ=255)                       aTTGCACCATGCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTCGTGGAACTGTAagag                                                                                                                                                                                              <  2:348228/251‑1 (MQ=255)                          gCACCATTCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGa                                                                                                                                                                                            <  1:345763/250‑1 (MQ=255)                                        gAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGa                                                                                                                                                                             <  1:440890/251‑1 (MQ=255)                                                     ttttGCGGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCATAATGGCGTGTTTGCGCGGGTGTTACGCGATCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTATTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCg                                                                                                                                                                  <  1:518978/249‑1 (MQ=255)                                                                 gggAAATACAGCTTTATAATCAATCGCCGGAAGATAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTGTTGAGCAGAATGGCGTGTTTGAGGGGGTGTTACGCGAGCTGGGGTGTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAAGCCGGCAGGATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGGACGGGAGTAGTAAAAATGGATTTCTTAGGTCGGttactgggtggagacgcta                                                                                                                                                    <  2:550549/251‑18 (MQ=255)                                                                         cAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGGTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGcc                                                                                                                                            <  2:213906/251‑1 (MQ=255)                                                                                      aatcGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCAt                                                                                                                                                                                                                                          <  2:450176/144‑1 (MQ=255)                                                                                      aatcGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGtt                                                                                                                               <  1:279622/251‑1 (MQ=255)                                                                                             ggAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTTCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGAtc                                                                                                                        <  1:577051/251‑1 (MQ=255)                                                                                                               aGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCGCGTGCAGACCACGCcac                                                                                                      <  1:100287/251‑1 (MQ=255)                                                                                                                    gtcgtGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGga                                                                                                 <  1:17973/251‑1 (MQ=255)                                                                                                                         ggCGGATACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGGGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGATAGGGCGCGGAGGGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGGTTGCTGATGTCGGTTTCGGGGGGCAGACGCTAACAGCGCCGATTCGGTTAGTTTCCGCGCTCGTGCAGACCCCGCCACCAGGAGAGt                                                                                             >  2:406594/1‑250 (MQ=255)                                                                                                                           cGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATc                                                                                          >  1:100195/1‑251 (MQ=255)                                                                                                                           cGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCAGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCCCACGGCGAGTATc                                                                                          >  2:300123/1‑251 (MQ=255)                                                                                                                                  tGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGgttgtt                                                                                   >  1:14903/1‑251 (MQ=255)                                                                                                                                                tGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGtgatg                                                                     >  1:59822/1‑251 (MQ=255)                                                                                                                                                   cGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACGGGAAGAGGAAAAATGGATTGCTGATGTCTGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGAtt                                                                  <  2:142307/251‑1 (MQ=255)                                                                                                                                                        ttGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGgtg                                                             >  1:430085/1‑251 (MQ=255)                                                                                                                                                               ggTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTAGCAAATCCGTCAGCATTACCGCCGCGCACCCAGCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACTCGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCa                                                        <  1:534446/249‑1 (MQ=255)                                                                                                                                                                  gtTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACAACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGttt                                                    >  2:214208/1‑250 (MQ=255)                                                                                                                                                                  ggtACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGttt                                                    <  2:176145/248‑1 (MQ=255)                                                                                                                                                                   ttACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGATTGCTGTTGGAGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGCGGGTGATGATTGGGTGTTGCAGTTTa                                                   >  1:211518/1‑250 (MQ=255)                                                                                                                                                                     aCGCGGGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAat                                                 <  2:139285/250‑1 (MQ=255)                                                                                                                                                                        cgAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGGTGCAGTTTAatcatc                                             >  2:126151/1‑251 (MQ=255)                                                                                                                                                                                      aCGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTCCCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACAGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGc                                 >  1:49426/1‑249 (MQ=255)                                                                                                                                                                                       cGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAgg                              >  2:362798/1‑250 (MQ=255)                                                                                                                                                                                           cGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAGTCGa                           <  1:474066/250‑1 (MQ=255)                                                                                                                                                                                                  tGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATGGGCAGTCGATGTAcc                     <  1:207676/249‑1 (MQ=255)                                                                                                                                                                                                       gggCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAACTGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGGTTAATCATCATCCGCATTGGCAGGCGATGTACCGTTTTgg              >  2:287403/1‑250 (MQ=255)                                                                                                                                                                                                        ggcgctgagtgtttacAAATCCGTCAGCATTACCGCCGCGCACCCATCGGTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTGTCGGTGGGCAGACGCAACCCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAGTCGATGTACCGTTTTGAt             <  2:14379/236‑1 (MQ=255)                                                                                                                                                                                                         gcgcGAAGAGTTCTCAAATCCGTCAGCGTTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGATAACCGCGCCGCTTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGAATCGGTTGTTGCAGGAGGGTGAGGATTGGGTGTTGCAGTTTAATCATCATCAGAATTGGCCGTCGATGGACCGTTTTGAtt            >  2:358388/1‑250 (MQ=255)                                                                                                                                                                                                                    tATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAGTCGATGTACCGTTTTGATCTCTGCGagca  <  2:100195/250‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               CGTGCATTGCACCTGAAACACAATTGCACCATTCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGCCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAGTCGATGTACCGTTTTGATCTCTGCGAGCA  >  NC_000913/1534099‑1534559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑