Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,531,318	C→T	intergenic (‑814/‑498)	yncO ← / → ydcC	protein YncO/H repeat‑associated putative transposase YdcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,531,318	0	C	T	100.0%	57.0 / NA	22	intergenic (‑814/‑498)	yncO/ydcC	protein YncO/H repeat‑associated putative transposase YdcC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

AAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTCATTGGGTGTTAGATGTAAAAATGAATG  >  NC_000913/1531078‑1531538                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                             aaGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGATTAGTACGCCGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACtttt                                                                                                                                                                                                                    >  1:522504/1‑251 (MQ=255)                                   gTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTaaaaa                                                                                                                                                                                   <  2:441582/250‑1 (MQ=255)                                   gTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTaaaaa                                                                                                                                                                                   <  1:384566/250‑1 (MQ=255)                                               tCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGc                                                                                                                                                                      >  1:408740/1‑251 (MQ=255)                                                      gAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGCGGAAATTACATCATGCATTCGAGGCCACATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGCTTAGTAAGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTTATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTc                                                                                                                                                                >  1:548185/1‑250 (MQ=255)                                                         gATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCAGAGTGTCAGTAACGTAACGTCTGAACGTTTGTGATTTTGAATTCGAATTGAAGGGATTAAAAAAGCGTTGTGTAGCATGGTCATTc                                                                                                                                                            <  2:387077/251‑1 (MQ=255)                                                            cAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAgg                                                                                                                                                         >  1:156396/1‑251 (MQ=255)                                                                                               aaGGCAATCAGGGGAAATGACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAAGGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAg                                                                                                                      <  2:326542/251‑1 (MQ=255)                                                                                                        aGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGata                                                                                                             <  1:393981/251‑1 (MQ=255)                                                                                                                   catcatGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTtcat                                                                                                  >  2:261786/1‑251 (MQ=255)                                                                                                                      cattcATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCaa                                                                                               <  2:408740/247‑1 (MQ=255)                                                                                                                      catGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTtcatca                                                                                                >  1:323891/1‑250 (MQ=255)                                                                                                                               gAGGAAAAAGTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGAAGACGGGGTAAGCATCAGATATTATATTTCATCAAATGATATgg                                                                                      >  2:412664/1‑251 (MQ=255)                                                                                                                                       aTTTTCTGTTAAAGTGTTTTCTAATTATAAAGGCGATTCGGTTAGTACGGAGGAGATTAGTCATGGAAGAAAAGAAAAACGTTTGCATATTGTCAGTTACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATTGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTaaa                                                                              <  1:530807/251‑1 (MQ=255)                                                                                                                                           ccTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGATTAGTACGCAGGAGATAAATCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAACGAAGATAAATCAGCAGAAGGTGTAAGCATCATATATAATATTTCATCAAAGGGTATGGATGCTAAAGAAt                                                                          >  1:354537/1‑251 (MQ=255)                                                                                                                                                aaatgttgtcacTAATTATAAAGGCGATTCGTTTAGTCCGCACGAAATTAGTCACGGAAGAAAAGAAACTCGCTTGCATATTGCCCTTAACGTAACGTCTGAACCTTTGTGATTTTGTATTCGAACGTGAGGGATTAAAAAAGCTTTGTGTAGCTTTGTCATCCAGGCAGAAGAAAGAAGATATATCGGAAGCAGGAGAAAGCATCCGAGATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGc                                                                      <  1:584375/239‑1 (MQ=255)                                                                                                                                                                                  aGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGTATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGcaca                                   <  2:347971/251‑1 (MQ=255)                                                                                                                                                                                         aGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTc                             >  1:1642/1‑250 (MQ=255)                                                                                                                                                                                                 aGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGGAACGTAACGTCTGAACTTTTGGGAGTTGTAAGTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGAGATGGATGGTAAAGAATTTGCACATGCTATCATAGCGCACTGGCTGATCGAGCACAGTCTTCATTGGgtgt                    <  2:258874/251‑1 (MQ=255)                                                                                                                                                                                                                aaaactcggttgtatatTGTCAGTAACGTAACGTATGAGCGTTTGTGATTTTGAATTCGAATTGAAGGTATTAAAAAATCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAATGTGTAAGCATCTGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATTCTATCAGAGCGCACTGTCTTATCGAGCACAGTCTTCATTGGTTGTTAGATGTAAAaatga     <  2:423202/238‑1 (MQ=255)                                                                                                                                                                                                                  aaCACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGAAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTCATTGGGTGTTAGATGTAAAaatgaat   >  2:420510/1‑251 (MQ=255)                                                                                                                                                                                                                   acacGTTTGCATATTGTCAGTAACGTAACGTCTGTACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGGAGCATTGTCATACAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTCATTGGGTGTTAGATGTAAAaatgaatg  <  1:486076/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                             AAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTCATTGGGTGTTAGATGTAAAAATGAATG  >  NC_000913/1531078‑1531538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑