Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,068	A→G	V45A (GTA→GCA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,068	0	A	G	100.0%	97.9 / NA	32	V45A (GTA→GCA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/20);  total (12/20)

GCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTGATA  >  NC_000913/155828‑156307                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                gCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGac                                                                                                                                                                                                                                          <  1:421901/248‑1 (MQ=255)  cATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCGCGTTTGGCATCAATACGTGATACTGGCGGCGTAGCAGTAAAAGGGACGGTAATACTGCCAGGCACAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACtt                                                                                                                                                                                                                                      <  1:567471/251‑1 (MQ=255)       ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACttt                                                                                                                                                                                                                                     <  2:166292/247‑1 (MQ=255)                  aaCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACGGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACTCTTTTTTGATCGCTTTtat                                                                                                                                                                                                                      <  2:543984/251‑1 (MQ=255)                      gccAGGACGTTAACCCAGACCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATCAATTTGATTGCTTGCCCACGTTTGGCATCAATCCGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGCCATCGCCAGTATCTCACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTGTTTCAGCGCTTTtatat                                                                                                                                                                                                                    <  2:197689/248‑1 (MQ=255)                                                   tctGTCTTTAGGCAGTTAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTcc                                                                                                                                                                                      <  2:170792/250‑1 (MQ=255)                                                                            gctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCATCGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATGACGCGAGTTCCCGAAATGACAATGGCCGCAATAGAAGATGAACTAAAAGCCAt                                                                                                                                                            <  2:465838/251‑1 (MQ=255)                                                                                tgtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATAc                                                                                                                                                          >  1:429072/1‑249 (MQ=255)                                                                                       tAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTGCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAAGGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACg                                                                                                                                                  <  2:290553/250‑1 (MQ=255)                                                                                           ttGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGc                                                                                                                                              >  1:43465/1‑250 (MQ=255)                                                                                                   ttGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCGCAGCGTTGTCATCGCCAGTATTTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCGGTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAGCTAAAAGCCATACAGGTTACGAAGCTTAAAGCt                                                                                                                                      <  2:43465/250‑1 (MQ=255)                                                                                                      cccACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGACCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtgttg                                                                                                                                  <  2:79959/251‑1 (MQ=255)                                                                                                                                    cggcgTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaa                                                                                                                   >  1:403267/1‑236 (MQ=255)                                                                                                                                    cggcgTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaa                                                                                                                   <  2:403267/236‑1 (MQ=255)                                                                                                                                          aGCAGTAAAATGGATTGTAATACCGCCAGGCTCAGCGTTTTCATCGCTAGTATCTAACCAACTCTGGACAAGCAACTGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACTCTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAAGGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCAGATAGCTGTTGTATGTTTGGTGTTAAGAAACACAACCCATCCCTGa                                                                                               <  2:383534/250‑1 (MQ=255)                                                                                                                                             aGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGAt                                                                                              >  2:179609/1‑248 (MQ=255)                                                                                                                                             aGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATaga                                                                                           <  2:462323/251‑1 (MQ=255)                                                                                                                                             aGTAAAAGGGACCGTAATACTGGCAGGCTCGGCATTGTCGAAGCAAGTATCTCACCAACTCTGGACAAGCAATGGGTTATTCTCTTAATTGTCCAGACGTGCGTTGACACTATTGTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGACCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTTTGTTTGGTGTTAAAAAACATAAACAATCCCTGATaga                                                                                           <  2:18589/251‑1 (MQ=255)                                                                                                                                                    gggAATGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCCGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAAGGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGttaatt                                                                                    <  2:449682/251‑1 (MQ=255)                                                                                                                                                                       gCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATaa                                                                 <  1:421889/251‑1 (MQ=255)                                                                                                                                                                         tCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATaaa                                                                >  1:168173/1‑250 (MQ=255)                                                                                                                                                                              gTTGGCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCACTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGaa                                                          <  1:355341/251‑1 (MQ=255)                                                                                                                                                                                tGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATg                                                                                                                              <  2:82518/181‑1 (MQ=255)                                                                                                                                                                                tGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATg                                                                                                                              >  1:82518/1‑181 (MQ=255)                                                                                                                                                                                  tCATCGCCAGACTCTAACCAACTTTGGACAAGTAACGGGTTATTCCCTTTATTTTCCAGACGAGCGTTGACACTATTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATATCCGCAGTAGACGATGAACTGAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACAATCCCTGATAGAGTTAATTAACAAAGGGATTAATATAAAATCGAAatt                                                       >  2:58735/1‑250 (MQ=255)                                                                                                                                                                                           gTATCAAATCAACTCTGGGCAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACCCATTTTTGACCGCTTTTATATATTAAGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGATGTTGTATGTTTGGTGTTAAAAAACCTAAACAATCCCTGCTAGAGTTAATTAAAACCGGGAATACTATAAAATAGAAATTATTATTCCCt                                             >  2:564478/1‑251 (MQ=255)                                                                                                                                                                                                 aaCCAACTCTGGACAGGCACCGGGTTGTTCCCTTCATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGCGTCCCCGAAATGACAATGTCCGCAATAGCCGATGAACTAAAAGACATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTatat                                       >  2:251464/1‑251 (MQ=255)                                                                                                                                                                                                      aCTCTGGACAAGCAACGGGGTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTAAGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATTAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATattcatt                                  <  1:337591/251‑1 (MQ=255)                                                                                                                                                                                                           ggACAAGCATCGGGTTATTCCCTTTATTTGCCAGACGTGCGTTGACACTTTTTTGATCGCGTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATTCCTTATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAAGTATTATTCCCTGTATATTCATtcaa                               <  2:573567/249‑1 (MQ=255)                                                                                                                                                                                                                      gggTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTgg                  >  2:472113/1‑251 (MQ=255)                                                                                                                                                                                                                          tATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCACTAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGa               >  1:217173/1‑250 (MQ=255)                                                                                                                                                                                                                                       ttCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAACAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTGATa  >  2:196202/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                GCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTGATA  >  NC_000913/155828‑156307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑