| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,604,350 | 0 | C | T | 30.6% | 49.2 / 11.2 | 36 | S64F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (8/17); new base T (11/0); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.46e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAAC‑GCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACA > NC_000913/4604108‑4604586 | cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCTCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATga < 2:201552/251‑1 (MQ=255) gTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTg < 1:291040/251‑1 (MQ=255) aaGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAgcg < 1:297199/251‑1 (MQ=255) taacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGTGAAGTGAGCGCCGAGAggg > 2:336571/1‑251 (MQ=255) aacctgcattatGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATTCCTATACCAGTCATGAGTCATTTCAGGATGCCATGTCGCGGAGCTCGTTTGCGGGGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAggg < 1:267453/239‑1 (MQ=255) tCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTAGCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCtt < 2:309276/249‑1 (MQ=255) gTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAAGGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGATCGCAGAGAGGGATTATCTTGc < 1:203380/248‑1 (MQ=255) aTGGAGATGACGCCGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGACAGGAGTCATTTCAGGCTGCCATGTCGCGGATCTAGTTTGCGGCGGTCCTGTTTTTTTTTTCTT‑CCATGAGAAGTGAGAGGCGAGAGGGAGTATcattgctgactt > 2:353531/1‑240 (MQ=255) aTGGAACACAACCGCATTAAGAAGAGAAATGTCGAACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGCAACCCTTATAAGCTCCATACCTATCCCAGTCAGGAGTCATTTCAGGATGCCAGGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTG‑CCATGGGAAGAGAGAGCCGTGAGGGGTTATTTTGGTTGTCTGGACTGGCggtttaggt > 2:248523/1‑243 (MQ=255) caGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATACGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTTTTTTTTTT‑CCATGTGAAGTGAGCGCCGAGAGGGATTTTTTTGGCTTACTTAACTGGCGGTTAAGTTTCCGCGt > 2:164363/1‑250 (MQ=255) aagaagAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGttt < 1:454972/251‑1 (MQ=255) aagaagAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTCTT‑CCATGAGGAGAGAGAGCGGAGGGGGATTATCTTGCCTGACTGAACAGGCGATTTAGTTTTCGGGGCCCC‑GGCGttt > 2:397002/1‑251 (MQ=255) gaagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGCGAAGTGAGCGCAGAGGGGGATTATCTTGCCTGACTGAACTGTCGGTTAAGTTTCCGCGTACCC‑GGCgatttt > 1:319536/1‑246 (MQ=255) tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCTTGAGGAGTGGGCCGAGCGAGGGGTTTTTTTGCCCGGATGGACTGGCGGTTAAGTTTCCGCGGACCCCGGGGTTT‑GTTTTTTCGGATGGTGATAATGaaacgcagagcgggagggcatacgcct > 1:452516/1‑226 (MQ=255) tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTc < 2:452516/230‑1 (MQ=255) gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc < 1:147297/251‑1 (MQ=255) agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCCCCATTCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCTGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTAGCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGTTGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc < 1:566511/251‑1 (MQ=255) gTTTATTCAGAGAGTTTGCGGGTCACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGCTGCCATGTCGCGGATCTCGTTTGCGGCGGTTACTTTTTCTTTTTTTGTCCTTG‑GAAGTGAGCGACGAGGGGGCTTATTTTTCCTTTATGCACTGGCGATTAAGTTTTCCCGGACCC‑GGCGTGTTGTTTTTGTGGATGATGGTAATGAAACGCGGCTGATTGGTTcttttttggcatccacc > 2:87970/1‑235 (MQ=255) aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg < 1:336571/251‑1 (MQ=255) ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGTAATGGCGATTAAATTTCCGCGTAACC‑GGCGTTTAGTTATTGCGGGTGATGATATTGAAGCTCGGCTGTTTGGTTTATTTTCTCCATCACCGCTTGGCGGTGTAttt > 1:440705/1‑250 (MQ=255) ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTaa < 2:160863/251‑1 (MQ=255) aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTT‑CCCTGCGAAGGGAGAGCCGAGAGGGGGTATTTTTCATGGCTGACCTGGCGCATAAGTTTTCGCGTCACC‑GCGGGTTAGTTTTTGCGGAGGGTGTTATTGAAGATCGGCTGATTGGGTCGTTGTCGCCATCCCCGCTGGGGGGGGTATTTAGTaaa > 1:294146/1‑249 (MQ=255) aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCCTGAGAAGTGAGCGCCGAGGGGGGTTTTCTTGCCCGGCTGGAATGGCGATTAAGTTTTCGCGTACCC‑CGCGTTTTGTTTTTGCGGATGATGATATTTAAGCTCGGCTGGTTGGTTTCTTTTCGCCCTCACCCCTGGGCGGGGTATTTAGTaaaac > 1:512899/1‑249 (MQ=255) cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGt < 1:409792/251‑1 (MQ=255) ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCAAGAGAAAGGAGCGCAGAGAGGGGTTATATTTCCTGACTGAACTGGGGCTTAAGTTTCCTCGTACCC‑CGCGTTTAGTTTTTGAGGAAGATGATATTTAAGCTCGGATGATTGGTTTCTTGTAGCCATCACCGCTGGACGGGGTATGTAGTAAAACGtt > 1:368093/1‑250 (MQ=255) ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCCTGAGCAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCC‑GGCGTTTTGTTATTGCGGGTGGTTATATTGAAGCTCGGCCGATTGGTTCATTGTCGCCATCACCGCTGGGCGGGGGATTAAGGAAAGCGTCAAC‑GTTgg > 1:338317/1‑248 (MQ=255) ccATACCTATACCAGGCATGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATGAAGTAAAGCTTCAAC‑GTTGgag < 1:443435/250‑1 (MQ=255) cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTT‑CCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTTAACTGGCGATTAAATTTCCGCGTACCC‑CGCGTTTTGTTATTTCGGGTGATGATTTTGAAGCTCGGCTGGTTGGTTTATTTTCGCCCTCCCCGCTGGGCGGGGGATTAAGTAAAGCGGCAAC‑GTTGGGGAt > 1:318271/1‑251 (MQ=255) gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCCGGGAGGGGTTATCTTGCCTGGCTGAACTGGGGCTTTAGTTTCCGCGTACCC‑GGCGTTTTGTTTTTGCGGATGGTGATATTTAAGCTCGGGTGATTGGTTCATTTTCGCCCTCCCCGCTGGACGGTGTATTAAGTAAAACGTCAAC‑GTTGGAGGTTTTTTATCCGGAAATCtt > 1:129571/1‑251 (MQ=255) cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑GCATGGGAAGGGAGCGCAGAGAGGGGTTATTTTGCCTTACTGAACTGGCGGTTAAGGTTCCGCGTAACC‑GGCGGTTTGTTTTTGCGGGTGATGATATTTAAGCTCGGCTGAGTGGTTCATTTTCGCCATCCCCGCTGGGCGGGGGAATAAATAAAGCGTCAAC‑GGTGGAGATTTTTTATCCGGAAATCtttttt > 2:94062/1‑250 (MQ=255) aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTT‑CCATGGGAAGTGAGCGCCGAGAGGGGTTATCTTTCCTGACCGAACTGGCGATTAAATTTCCGCGGACCC‑GGCGTTTTGTTATTTCGGGTGATGATATTGAAGCTCGGCTGGTTGGTTTCTTGTCGCCATCACCGCTGGGAGGGGTATTTAGTAAAACGTCAACAGTTGG‑GGTTTTTCATAAGGGACTCttttttt > 1:342140/1‑249 (MQ=255) ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAAC‑GTTGGAGATTTTTCATCAGGAACTCTTTTTGTc < 2:512899/250‑1 (MQ=255) gCCATGGCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTGCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCTCCATCACCGCTGTACGGTGTATTAATTAAAGCGTCAAC‑GTTGGAGATTTTTCATCAGGAACTCTTTTGGTCATTAAATg < 1:87970/250‑1 (MQ=255) gcggcgGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAAC‑GTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgacc < 1:27812/250‑1 (MQ=255) ggcggTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAAC‑GTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgaccgac > 1:335086/1‑251 (MQ=255) aTTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAAC‑GTTGGAGATTTTTCATCAGTAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACa > 1:411592/1‑250 (MQ=255) | CGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC‑GGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAAC‑GCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACA > NC_000913/4604108‑4604586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |