Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0690GC30.4% 79.3 / 14.2 56P133R (CCG→CGG) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (15/24);  new base C (17/0);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 6.02e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTG  >  NC_000913/4255820‑4256315
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                 
aGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGATCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑CT‑CGCCCTTTT‑TAT‑G‑CCCCgg                                                                                                                                                                                                                                                                  >  1:11028/1‑251 (MQ=255)
  aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGGTCCC‑TT‑CGCCCTTTT‑TAC‑G‑CCCCGgcg                                                                                                                                                                                                                                                                >  1:384271/1‑251 (MQ=255)
       gACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGcgcgc‑g                                                                                                                                                                                                                                                           <  1:347048/250‑1 (MQ=255)
         cGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑Acc                                                                                                                                                                                                                                                       <  1:58027/251‑1 (MQ=255)
               ggCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCCGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑GACCCCgggcccgacccaaaat                                                                                                                                                                                                                                                    >  1:261858/1‑237 (MQ=255)
                  aaaCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGGTCAC‑CT‑TGCCTTTTT‑TAC‑G‑CCCCCGCGCCC‑G‑ACCAAACA‑TCAc                                                                                                                                                                                                                                             >  1:120200/1‑251 (MQ=255)
                            cTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑TAC‑G‑CCCCCGCCCGC‑G‑ACCCAACA‑TCACC‑GAC‑ACTGGc                                                                                                                                                                                                                                 >  1:271756/1‑251 (MQ=255)
                                      cAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGGTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCCGCGCGC‑G‑ACCAAACA‑TCCCC‑gcccctcgccccacctgc                                                                                                                                                                                                                         >  1:534312/1‑233 (MQ=255)
                                          cGTTGACGGGCAAAGTGCATCCGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCTCAGCGAAACTGACGTCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATc                                                                                                                                                                                                                   <  1:108459/251‑1 (MQ=255)
                                                 gggCAAAGTGCATCCGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑ttt                                                                                                                                                                                                          <  2:18808/251‑1 (MQ=255)
                                                   gcaaaggtcattcgcgcccggggccccAGTTTGTGAGCGATATTTTTACCCGTCCCGTGTTCATCCGCCATACGGCGCCGCGAAACTGACGGCTAGAAACTCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAATTTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCTCCATCTGCTCATCC‑AGA‑TTTgg                                                                                                                                                                                                        <  2:383803/226‑1 (MQ=255)
                                                                      cgcgcgCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGGTCTA‑TT‑CGCCTTTTT‑CAC‑G‑CCCCCGGGCGC‑G‑AACCAACA‑TCCCC‑GAC‑CCCGGCACCATCTGCACCTCC‑CAA‑TTTGGGGTGCTACGGTGCaaaacg                                                                                                                                                                                     >  2:519585/1‑248 (MQ=255)
                                                                            caagtgtcggAGCGATAGTTTTATCCGGGCCGTGTCCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGGCGCGACCGTGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGGTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAg                                                                                                                                                                               <  2:498244/242‑1 (MQ=255)
                                                                                  tcggggCGATAGTTTTACCCGTGCCGTGTTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTg                                                                                                                                                                          <  2:529034/245‑1 (MQ=255)
                                                                                       gCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑C‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑CCTTGCACCCTCTGCACCACC‑AGA‑TTTGGGTTGCTACGGTGCAAAACGAGATAGGCGTgggac                                                                                                                                                                      >  1:230507/1‑246 (MQ=255)
                                                                                        cGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAATGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GACGTTAAGCATACGCAGCGGCGGGCTCAC‑TTTCCCCTTTTT‑‑TC‑C‑CCCCCGCGCGC‑G‑ACCCAACA‑TCACC‑CACCAC‑GGCACCCTCTTCACATCC‑AAC‑TTTGGGGTGCTACCGTGCCAATCTAGGTAGTAGTGG‑AAc                                                                                                                                                                     >  1:336841/1‑248 (MQ=255)
                                                                                             gTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAAt                                                                                                                                                             <  1:208376/251‑1 (MQ=255)
                                                                                                    ccgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGCCGGCGAGAAACGCACAACACTGTCGCGACCGAGCCACAGTACAGCGAACAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGACGGGGACAC‑TT‑CGCCCTTTT‑CCC‑G‑CCCCGGCGCGC‑G‑ACCCA‑CACTCACC‑CGC‑ACTGGCCCCATCCGCCCCACC‑CGC‑TTTTGGGTGCTGCGGTGCAAAACGAGATAGTCGTGC‑GACAGCTTAATAGActct                                                                                                                                                      >  2:150354/1‑251 (MQ=255)
                                                                                                     cgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑TAC‑G‑CCCCCGCGCGC‑G‑ACCAAACA‑TCACC‑CAC‑ACTGGCACCATCTGCACACCC‑AGA‑TTTGGGTTGCTACGGGGCAAATCTGGATAGTCGCGG‑AACAGCTTAATAAACtctt                                                                                                                                                     >  1:470967/1‑251 (MQ=255)
                                                                                                            tgtTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAA‑TT‑CGCCTTTTT‑CTA‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TAACC‑GCC‑ACAGGCACCCTCTGCACCTCC‑AGC‑TTTTGGGTGCTACGGTGCCAATCGAGATAGTAGTTG‑AACAGCttcattacatcttctttcgg                                                                                                                                              >  1:16038/1‑233 (MQ=255)
                                                                                                                cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTg                                                                                                                                                                                                         <  1:551671/189‑1 (MQ=255)
                                                                                                                cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCgt                                                                                                                                           <  1:404828/250‑1 (MQ=255)
                                                                                                                     gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTTCC‑TT‑CGCCTTTTT‑CCC‑G‑CCCCCGCGCGC‑G‑ACCACACA‑TCACC‑GAC‑ACTGGCACCCTCCGCCCATCC‑CGAATTTGGGT‑GCTACCGGGCAAAACGAGAAAGGCGTTG‑GACAGCTTAATAGACTCTTCCTTCTGCGTGT‑AAt                                                                                                                                     >  1:362578/1‑250 (MQ=255)
                                                                                                                         tACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCTGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑Tg                                                                                                                               >  1:24726/1‑251 (MQ=255)
                                                                                                                                 aGCGAAACTGACGGCGAGCAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAA‑G‑CCCCGGCGCGC‑G‑ACCCAACA‑TCACC‑CAC‑CCTGGCACCATCTGCACATCC‑AAA‑GTTGGGGTGCTACGGTGCAAATCGCGATAGTCGGGG‑GAAAGATTAATAGACTATTCTTTCGGCGTGG‑AAACGA‑TGAACACAcg                                                                                                                       >  1:448303/1‑251 (MQ=255)
                                                                                                                                      aaCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTTAC‑TT‑CGCCTTTTT‑TAC‑G‑CCCCCGCGCGC‑G‑ACCCAACA‑TCACC‑CAC‑ACTGGCACCCTCTGCACATCC‑AGA‑TTTTGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATATACTCTTCTTTCTGCGTGT‑AATAGG‑TGAACACACGCGGcc                                                                                                                  >  1:298604/1‑251 (MQ=255)
                                                                                                                                      aaCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGcc                                                                                                                  <  1:224741/251‑1 (MQ=255)
                                                                                                                                         tGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGATCAA‑CT‑CCCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑GCCCAACA‑TTACC‑GAC‑ACCAGCACCATCTTCCCATCC‑CGA‑TTTTGGTTTCCACCGTGCAAATCGAGATAGTGCTGG‑GACAGCTTAATAAACTCTTCTTTCGGCG‑GTGATTAGG‑TGAacacccaccgcccgc                                                                                                               >  1:426683/1‑240 (MQ=255)
                                                                                                                                               cGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTTAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCCGCGCGC‑G‑ACCAAACA‑TCACC‑CAC‑ACCGGCACCCTCTGCCCATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGAGG‑AACCGCTTAATAGACTCTTCTTTCTGCGTGT‑AATTGG‑GGAAAAAACGCGGCCCGCCGtga                                                                                                          >  1:359084/1‑250 (MQ=255)
                                                                                                                                               cGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑TGCCCTTTT‑TAC‑G‑CCCCCGCGCGC‑G‑CCCCAACA‑TCACC‑CCC‑ACCAGCCCCACCTGCACATCC‑CGA‑TTTTGGGTGCTACCGTGCAAAACGCGATAGTTGGTG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGGGTGAA‑ACACGCCGCcccccgcga                                                                                                          >  2:128903/1‑247 (MQ=255)
                                                                                                                                                gagaAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑TCCCTTTTT‑TAC‑C‑CCCCGGCTCGC‑G‑CCCAAACA‑TCACC‑GGC‑ACTGGCCCCATCTACACAAAC‑AGC‑TTTGGGTTGCTTAGGGGCAAATCGAAAGAATCGTGG‑GAAAGCTTAATAAACTCTTCTTTTCGCTCGG‑AAAAGG‑TGAACACACGCCGCCCCCCGcgaa                                                                                                         >  2:427422/1‑250 (MQ=255)
                                                                                                                                                       gCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAA‑TT‑TGCCTTTTT‑CAC‑G‑CCCCCGCGCGCCG‑ACCAA‑CA‑TCACC‑CAC‑ACGGGCACCAACTGCACCACC‑AGA‑TTTTGGGTGCTACGGTGCAAATTGAGATAGTCGTGGGAAC‑GCTTTATATCCTCTTTTTTCGGCGTGG‑AATAGG‑TGAAAACACCCGGcccccccgggatgaaaccc                                                                                                 >  1:142600/1‑235 (MQ=255)
                                                                                                                                                          aaaaacggccgcgACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCGGGGGCGGGTTCAC‑TT‑CGCCTTTTC‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑CCACC‑GAC‑ACTGGCACCATCGGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAg                                                                                             <  1:58293/242‑1 (MQ=255)
                                                                                                                                                              agtGTCGCGCCCGAGCAACAGTAAAGCGAAAAATTTCT‑TACCGCCGTAAAGCATCCGCAGCGGGGGGTTCTC‑TG‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑C‑ACAAAACA‑TCCCC‑GAC‑ACTGGCACCATCTGCCCACCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTGGTGA‑AACAGCTTAATAAACTCTTCTTTCGGCTTGT‑AAAAGG‑GGAACACACGGGGCCCGCCGTGAATGAACAC‑ATAGcgcg                                                                                         <  1:178910/249‑1 (MQ=255)
                                                                                                                                                               cTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAA‑CT‑CGCCTTTTT‑TCC‑G‑CCCCCGCGCGC‑G‑ACCCAAAA‑TCACC‑GGC‑CCTGGCACCACCTGCACATCC‑CGA‑TTTGTGGTGCTACGGTGCAAAACCAGGTAGTCGTGG‑AACAGCCTAATAGACTCTTTTTTTGGCGGGT‑AATAAG‑TGTAAACACGCGGCCCGCCGTGAATGAACAC‑CTAACGCgg                                                                                        >  2:438054/1‑251 (MQ=255)
                                                                                                                                                                  cctcgcccgcgccacggtcaatagAAAAATTTCTGTCG‑GCCGTTAAGACCACGCGGCGGGGGGTTCAC‑TT‑CGCCTTTTT‑CCC‑G‑CCCCGGCGCGC‑G‑ACAAAACA‑GCCCC‑GAC‑TCTGGCACCATCTGCACATCC‑ATA‑TTGGGGTTGCTACGGTGCAAATCGAGAGAGTCGTGG‑ACCAGCTTAATAGACCCTTCTTTCGGCTGGT‑AATAGG‑TGAACACACGTGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAg                                                                                     <  1:304184/228‑1 (MQ=255)
                                                                                                                                                                     cgACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑A‑CCCCGGCGCGC‑C‑ACCCAACC‑ACACC‑CAA‑CCCGGCACCATCTGCCAATCCCAAT‑TTTTGGTT‑CTCAGGTGCAAATCCAGATAGTCGGGG‑AACAACTTAATAAACTCTTCTTTCGGCGGGG‑AATAGG‑AGGACACACCCGACCCCCCCGGAATGCACAC‑ATACCGCGGCAgca                                                                                   >  1:576590/1‑248 (MQ=255)
                                                                                                                                                                      gACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTTAC‑TT‑CGCCTTTTT‑TAC‑G‑CCCCCGCGCGC‑G‑GCCAAACA‑TCACC‑CAC‑ACTGGCCCCCTCTGCACCTCC‑CGA‑TTTGGGTTGCTACGGTGCAAATCTAAATAGTCGTGG‑GACAGCTTAATAGACTCTTCTTTTGGCGTGT‑AAAAGG‑GGAACACAcgc                                                                                                                      >  1:467110/1‑215 (MQ=255)
                                                                                                                                                                      gACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAA‑TT‑GCCCTTTTT‑CTC‑G‑CCCCCCCGCCC‑G‑AGCACCAA‑TCACC‑CCC‑ACTCCCACCATCTGCACATCC‑AGA‑TTTTGGTTTCTACCGAGCAAAACGAGATAGTCGGGG‑AACAGCTTAAAAGAATCTTCTTTCGGCGTGG‑AAGAGG‑AGAACACACGCGGACCGCCGTGAATGAGCAC‑ATAACGCGGccgaggg                                                                                 >  2:480594/1‑245 (MQ=255)
                                                                                                                                                                          gAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCTGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGCGAATGAACAC‑ATAGCGCGGCAGAAACGTGc                                                                             >  1:18269/1‑251 (MQ=255)
                                                                                                                                                                          gAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGGTCAC‑CT‑CTCCTTTTT‑TCC‑G‑CCCCCGCGCGG‑GGCCCAAAC‑‑TACCC‑CAC‑ACTTGCACCCTCTCCACATCC‑AGC‑TTTGTGGTGCTACGGTGCAAATAACGCTAGTTGTGG‑GACAGCATAATAGACTCTTCTTTCGGCGTGT‑AATATG‑GGAAAACAAGCCGGCCGCCCTGGATGAACCC‑ACAGCGCGGCAGGAGCGTgg                                                                             >  2:248485/1‑250 (MQ=255)
                                                                                                                                                                          gAGCCACAGTACAGCAAAAATTTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGTTTCCC‑TC‑CGCCTTTTC‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGc                                                                             <  1:67728/251‑1 (MQ=255)
                                                                                                                                                                           aGCCACAGTACAGCGACAAATTTCTGTCC‑GCCGTTAAGCATACGCAGCGGCGGGTTCCC‑TT‑CGCCTTTTT‑CTC‑C‑CCCCCGCGCGC‑G‑ACCCAACC‑TCCCC‑CAC‑CCCGGCACCATCTTCACCACC‑CCA‑ATTGGGGTGCTAACGTGGACAACCAGATAGTCGTGG‑ACAAACTTAATAGACTCTTTTTTCCGCGTGG‑AATAGG‑GGAACAAACAAGGCCCGCCGGGGATGAAACC‑AAAGGGCGGCAGGAGCGTGcc                                                                            >  2:196389/1‑251 (MQ=255)
                                                                                                                                                                                caGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTTAC‑TT‑CGCCTTTTT‑TCC‑G‑CCCCGGCGCCC‑G‑ACCAAAAA‑TCACC‑GAC‑ACTGGCACCCTCTGCCCATCC‑CGA‑TTTGGGTTGCTACCGTGCCAATCGAGATAGTCGTGG‑AAAAGCTTAATAGAATCTTCTTTCGGCGGGT‑AAATGG‑TGAACAACCCCGGCCCCCCCGGAATGAACAC‑ATACAGCGGCAGTAGCGTGCCGTCgg                                                                       >  1:334507/1‑250 (MQ=255)
                                                                                                                                                                                 aGTACAGCGAAAATTTTCTGTCC‑GCCGTTAAGCATCCGCAGCGGCGGTTTCCC‑TT‑CGCTTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑CCACC‑GCC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGAt                                                                      <  1:511804/251‑1 (MQ=255)
                                                                                                                                                                                         aaaaatTTTCTGTCC‑CCCGTGAAGCATCCCCGGCGGCGGGTCCCC‑TT‑CGCCTTTTT‑CCC‑C‑CCCCGGCGCGC‑‑‑ACCAAACC‑TCCCCCGAC‑ACTGGCCCCATCTGACCATCC‑AGA‑TTGGGGTTTCTCCGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTTT‑AATAGG‑GGAACACCCGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCg                                                              <  2:230507/250‑1 (MQ=255)
                                                                                                                                                                                          aaaaaTTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGATTAC‑TT‑TTCCTTTTTTCA‑‑G‑CCCCCGCGCGC‑G‑CCCCAACA‑TCACC‑GAC‑CCTGGCAACATCTTCACATCC‑AGA‑TTTGGGGTGCTACGGTGCCAATAGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCCGCGTGT‑AATAGG‑GGGACACACGCGGCCCGCCGGGAATGA‑CACCATAGCGCGGGAGGAGAGTGCCGTCGGTTTccctggg                                                             >  2:232994/1‑246 (MQ=255)
                                                                                                                                                                                                     gcatgCCGTAAAGCATACGCAG‑GGGGGGTTTACCTT‑CGCTTTTTT‑CCC‑G‑CCCCGGCGCGG‑G‑ACCAAAAA‑CCCCC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGGGCAAATCGAGATAGTCGTGG‑AACAGCTAAAGAGACTCTTCTTTCGGCGGGG‑AATAGG‑TGAACACACGGGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACggg                                                   <  2:85251/247‑1 (MQ=255)
                                                                                                                                                                                                       cccccGTTACGCATCCGCGGCGGCGGGTTCAC‑TC‑CGCCGTTTC‑CCC‑C‑CCCCGGCCCGC‑G‑ACCAAACA‑ACACC‑GAC‑ACTGCCACCACCTGCACATCC‑AGA‑TTTGGTTTGCTACGGTGCAAAGCGAGATAGGCGTGG‑AACAGCTTAAGAGACTCTTCTTTCGGCGTGT‑AATAGG‑TAACCACACGCGGCCCGCCGTGAATGAACCC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACTGGTc                                                 <  1:150354/248‑1 (MQ=255)
                                                                                                                                                                                                          ccGTAAAGCATACGCACCGGGGGTTTCAC‑TC‑CGCCTTTTC‑CCC‑C‑CCCCGGGGCCC‑G‑ACCAAACA‑CCACC‑GAC‑ACTGGCACCATCTGCACACCC‑AGA‑TTTGGGTTGCTCCGGTGCAAATCGAGATAGTCGGGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AAAAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAgg                                              <  2:197379/251‑1 (MQ=255)
                                                                                                                                                                                                               aaGCATACGCAGCGGCGGGTTCAC‑TT‑CCCCTTTTT‑CCC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑CCACC‑GAC‑ACGGGCCCCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGt                                         <  1:438054/251‑1 (MQ=255)
                                                                                                                                                                                                                ggCATACGCAGCGGCGG‑TTCCA‑TG‑CGCCCTTTG‑CCCCG‑CCCCGGC‑CGCCG‑ACCAAACA‑CCACC‑GAC‑ACTGGCACCACCTGCACATCA‑AGA‑TTGGGGTTGCTACGTGGAAAATCGAGATGGTCGGGG‑AACGGCTTAATAGACTCTTCTTGCGGCGGGA‑AATAGG‑GGAACACCCGGGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                                        <  2:121778/250‑1 (MQ=255)
                                                                                                                                                                                                                                        ccccttccccttttcccccccccGGCGCGC‑A‑ACCAACCA‑CCCCC‑TAC‑ACGGGCACCATCTGCACATCC‑AGA‑TTTGGTTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGCCTTTTCTTTCGGCGTGT‑AATAGG‑TGACCACCCGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgc                      <  2:470967/231‑1 (MQ=255)
                                                                                                                                                                                                                                              cTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATATGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTAGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGAAACTGGGCGCGCAACGTc            >  1:548633/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 ttt‑CAC‑G‑CCCCGGCGCGC‑G‑AACAAACA‑TCACC‑GAA‑ACTGGCACCATATGCACATAC‑CGA‑TTTGGGTTGCTACGGTGAAACTCGAGATAGTCGTGG‑AACAGCTTAATAGCCTCTTCTTTCGGCGTGT‑ACTAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCCGGCAAGTAATGTGCCAGACACTGGGCCAGCAACGTCAGc         >  2:366000/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  tt‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACCTCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGTGAATGACCAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAAAGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGc         >  1:362204/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    tgcccccccccGGCGGGC‑G‑CCAAAACA‑ACACC‑GCC‑CCGGGCACCATCTGCACCCCC‑AGT‑TTTGGGTTGCTAGGGTGCAAATCGAGATAGTCGTGG‑AACAGATTAATAGACTCTTCTTGCGGCGTGT‑AATAGG‑TGAACACGCGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAg          <  2:271756/243‑1 (MQ=255)
                                                                                                                                                                                                                                                           ccccGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGCGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTCACCGGTCAGGCAAGTCCTGTGCCAGACACTGAGCGCGCaccccccacaactcgc  >  2:323804/1‑236 (MQ=255)
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                 
AGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTAC‑GCCGTTAAGCATACGCAGCGGCGGGTTCAC‑TT‑CGCCTTTTT‑CAC‑G‑CCCCGGCGCGC‑G‑ACCAAACA‑TCACC‑GAC‑ACTGGCACCATCTGCACATCC‑AGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG‑AACAGCTTAATAGACTCTTCTTTCGGCGTGT‑AATAGG‑TGAACACACGCGGCCCGCCGTGAATGAACAC‑ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTG  >  NC_000913/4255820‑4256315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑