Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,174,359	IS5 (+) +4 bp	coding (205‑208/285 nt)	gatB ←	galactitol‑specific PTS enzyme IIB component

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2101749 =	NA (NA)	21 (0.360)	20/492	1.4	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	2174359 =	0 (0.000)					coding (208/285 nt)	gatB	galactitol‑specific PTS enzyme IIB component
*	?	NC_000913	= 2174362	0 (0.000)	49 (0.840)	46/492	0.3	100%	coding (205/285 nt)	gatB	galactitol‑specific PTS enzyme IIB component
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TAAGCCAATCACAAGGCCAATGCCAACAAAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCTTTTTATTGTTACAGAGGGATTTATTCGTCT  >  NC_000913/2174111‑2174606                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        tAAGCCAATCACAAGGCCAATGCCAACAAAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACt                                                                                                                                                                                                                                                        <  1:8243/250‑1 (MQ=255)          cacaAGGCCAATGCCAACAAAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggt                                                                                                                                                                                                                                               <  1:98578‑M1/250‑8 (MQ=255)              aaaacgataaccaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCGAACGGAATAGCGcc                                                                                                                                                                                                                                          <  2:453644‑M2/16‑1 (MQ=255)                   gataaccaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCCAAAc                                                                                                                                                                                                                                     >  1:552511‑M2/231‑251 (MQ=255)                    aTGCCAACAAAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtc                                                                                                                                                                                                                                    <  1:150160‑M1/251‑19 (MQ=255)                    aTGCCAACAAAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGAAACATAGTCAGAATTTTATTTTGTAATGCTACGATACCTATACCAGAAAAAAAAGGCATGCCGTGAACGAAggaaggtgcgaacaactc                                                                                                                                                                                                                                    <  2:406394‑M1/251‑19 (MQ=255)                          acaaAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTGCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGCAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATCCCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaattccctgat                                                                                                                                                                                                                              <  2:263283‑M1/251‑25 (MQ=255)                            aaaGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatat                                                                                                                                                                                                                            >  1:442783‑M1/1‑225 (MQ=255)                                ccAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgaga                                                                                                                                                                                                                        <  1:562855‑M1/251‑31 (MQ=255)                                 cAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagat                                                                                                                                                                                                                       <  1:226158‑M1/251‑32 (MQ=255)                                   aTCCCGATATGCCGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAAGGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGATGCGACACCGATGCCATAAATAAAAGGCACGCCGTGAACTAAagaaggggcgcacaagtccctgagattagatca                                                                                                                                                                                                                     <  2:519301‑M1/251‑34 (MQ=255)                                       attcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAgtg                                                                                                                                                                                                                 >  1:485419‑M2/211‑251 (MQ=255)                                         atatGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTTAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttg                                                                                                                                                                                                               >  2:476407‑M1/1‑212 (MQ=255)                                         atatGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttg                                                                                                                                                                                                               >  1:494580‑M1/1‑212 (MQ=255)                                                  cccGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctgga                                                                                                                                                                                                      <  2:142027‑M1/251‑49 (MQ=255)                                                            tcgggcggaccaaatgatacgtcagtgggagagagctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagacagccgagaagaatttccccgcttattcgcaccttccCGATCGGAAGATCGCCACAACTCAGATCCACTTTGTCAGTGTGGCATATCAAattcaccca                                                                                                                                                                                             <  2:50820‑M2/61‑4 (MQ=255)                                                                aTCGCCTGCCTTCATGCCTAATATGTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCTCCCCTGTAAGATAGTCAGAATTGTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTTAACTAAggaaggtgcgaacaagtcccggatatgagatcatgtttgtcatctgtagccatagaacaggg                                                                                                                                                                                        <  2:332362‑M1/251‑63 (MQ=255)                                                                  ggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacaggaataaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTGCGATCCGCTTTGGCAGTGGGGCATATCAAATACACACCAACCata                                                                                                                                                                                      <  2:560087‑M2/68‑1 (MQ=255)                                                                   gcctgcctTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGGCGAGAATATAACGAATGACTTCTGAAAACATAGGAAACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttc                                                                                                                                                                                     >  1:152168‑M1/1‑186 (MQ=255)                                                                        aatgatacgtcagtgggagagatctcactaaaaactggtgataacgccttaaatggcgaagaaacggtctaaagaggctgattcaaggcatttacgggagaaaaaatcggctcaaacattaagaaatgaaatgactgagtcagccgagaagaatgtccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGtt                                                                                                                                                                                <  1:352971‑M2/74‑1 (MQ=255)                                                                             aTGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGCGGTAGAGAATATAACGCATGACTTCTGAAAACATAGGTAACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCAGTGAACTAAggaaggagcgaacaagtacctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtc                                                                                                                                                                           >  2:548885‑M1/1‑176 (MQ=255)                                                                                  cagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCAt                                                                                                                                                                      <  2:15466‑M2/84‑1 (MQ=255)                                                                                   aaTATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATGTTGTAATGCTTCGATACCGATTCCAGAAATAAAAGGCATGCCGTGAACTAGggaaggtgcgaacaagtccctgatagtagatcatgtttgtcatctggagccatagaacagggttcatcatgagttatcaa                                                                                                                                                                      <  2:547273‑M1/250‑81 (MQ=255)                                                                                    atatTTTAGAAAAAATAATGATTACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGCCTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGACGTTAACTAAggaaggtgcgcacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaact                                                                                                                                                                    >  2:211206‑M1/1‑169 (MQ=255)                                                                                     tatTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggag                                                                                                                                                                                                     >  1:229181‑M1/1‑168 (MQ=255)                                                                                     tatTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggag                                                                                                                                                                                                     <  2:229181‑M1/217‑50 (MQ=255)                                                                                      gggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAc                                                                                                                                                                  >  2:92760‑M2/164‑251 (MQ=255)                                                                                        gagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccATAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAccc                                                                                                                                                                <  1:541477‑M2/90‑1 (MQ=255)                                                                                           aGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttc                                                                                                                                                             <  2:442783‑M1/251‑90 (MQ=255)                                                                                            gAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcg                                                                                                                                                            >  1:418118‑M1/1‑161 (MQ=255)                                                                                                aaaTAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCCGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccg                                                                                                                                                         >  1:115815‑M1/1‑157 (MQ=255)                                                                                                    aatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagt                                                                                                                                                    >  2:21108‑M1/1‑153 (MQ=255)                                                                                                        atgaCACTCGGCAGCATCACAGTAGGGCCGAGGTCGAGAATATAACGCATGAATTATGAAAACCTAGGTCACCCCAGTACGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAGAAGGCATGCCGTGAACTAAggacagtgcgaaaaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttaatcatgagtcatcaacttaccttcgcctacagtgaat                                                                                                                                                >  2:568502‑M1/1‑149 (MQ=255)                                                                                                            ggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACa                                                                                                                                            <  1:415307‑M2/110‑1 (MQ=255)                                                                                                                  aacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAat                                                                                                                                       <  1:92760‑M2/115‑1 (MQ=255)                                                                                                                      caacaccGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgc                                                                                                                                  <  2:457601‑M1/248‑117 (MQ=255)                                                                                                                       aTCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATGTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctg                                                                                                                                                                                                                                <  2:394474‑M1/156‑23 (MQ=255)                                                                                                                       aTCACCGTAAGGCCGAGGTCGAGAATATCACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaaaaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgcc                                                                                                                                 >  2:482203‑M1/1‑134 (MQ=255)                                                                                                                         cACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgt                                                                                                                               <  1:213652‑M1/251‑120 (MQ=255)                                                                                                                          aaattgcgaagaaacggtcgaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATAGCTCCAAAACTACGATCCACTGTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGTTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGAt                                                                                                                               <  1:384564‑M2/123‑1 (MQ=255)                                                                                                                          aaatggcgaagaaacggtctaactaggctgattcaaggcatttacgggcgaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgcgaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGAtt                                                                                                                              >  1:222734‑M2/128‑251 (MQ=255)                                                                                                                                   agaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGCTCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGAcaca                                                                                                                      >  1:206698‑M2/119‑250 (MQ=255)                                                                                                                                    cGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTAGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaagttgcgaacaagtccctgatatgagatcatgtttgtcatctgcagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagacaagaa                                                                                                                     >  1:258859‑M1/1‑121 (MQ=255)                                                                                                                                      aacggtccaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttagtcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCTCTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATGTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACTCAActc                                                                                                                  <  2:23528‑M2/136‑1 (MQ=255)                                                                                                                                       acggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAActct                                                                                                                 >  2:204239‑M2/115‑251 (MQ=255)                                                                                                                                         ggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaactgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCttt                                                                                                               >  1:29137‑M2/113‑251 (MQ=255)                                                                                                                                           gagaATATAACGCATGACTGCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccagagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagctccgtcagtccagaaaagagatt                                                                                                             <  2:70055‑M1/251‑138 (MQ=255)                                                                                                                                                aataaCGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggtgcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagtgattttctt                                                                                                        <  2:282897‑M1/250‑143 (MQ=255)                                                                                                                                                      gCATGACTTCTGAAAACATAGGTCCCCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATCCCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggggcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcatcagtaagcgccgtcagccccgaaaagcgattttcttgtcccg                                                                                                  >  2:416993‑M1/1‑103 (MQ=255)                                                                                                                                                        aTGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgc                                                                                                 >  1:9442‑M1/1‑101 (MQ=255)                                                                                                                                                                     acgggagaaaaaaacggctcaaacatgaagaaaggaaatgactgagtcagccgagaagaatgtccccgctgattcgcaccttccCTAACGTAATATCGCCAAAACTACGATCCACTTTGGCAGTTGTGCATATAAAATGCACACCATCCATATAGGTTTCTATTTCATTACCCCGACCCTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTGCTTCCGCCGCCATCGTAGAGGTc                                                                                   <  2:254‑M2/167‑1 (MQ=255)                                                                                                                                                                     aCATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggagcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattct                                                                                   >  1:225058‑M1/1‑88 (MQ=255)                                                                                                                                                                                    gTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacat                                                                    <  1:5010‑M1/251‑179 (MQ=255)                                                                                                                                                                                    gTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaac                                                                      <  1:21108‑M1/249‑177 (MQ=255)                                                                                                                                                                                     tAAGATAGTCCGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaacgagattttcttgtcccgcatggagcagattctgccatggcaaaacatg                                                                   >  2:465212‑M1/1‑72 (MQ=255)                                                                                                                                                                                          tAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggagcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagcccagaaaagagattttcttgtcccgcat                                                                                               >  1:375376‑M1/1‑67 (MQ=255)                                                                                                                                                                                          tAGTCAGAATTTTAGTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccagagaacagggttcatcatgagtcagcaacttacctgcgccgacagtgaattcagcagtaagcgccgtcagcccagaaaagagattttcttgtcccgcat                                                                                               <  2:375376‑M1/218‑152 (MQ=255)                                                                                                                                                                                                       gaaaggactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTg                                                  <  1:486290‑M2/200‑1 (MQ=255)                                                                                                                                                                                                           tGTAATACTACGATAACGCTACCAGACGTCAAAGACATGCCGTGAACTAAggaaggtgcgaacaagtccatgataagagatcatgtttgtcctctggagccatagaacagggttcatcatgagtcctcaccttcccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtactcgagccgtttt                                             >  2:535407‑M1/1‑50 (MQ=255)                                                                                                                                                                                                            gactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTc                                            >  2:157004‑M2/46‑251 (MQ=255)                                                                                                                                                                                                                tGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagctcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttacccc                                        >  2:270376‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                    tCGATACCGATTCCAGAAATAAAAGGCATGCCGTGAACGAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaag                                     <  1:416993‑M1/250‑210 (MQ=255)                                                                                                                                                                                                                           cgataccAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggaggaagtcatcgagccgttttaccccaaggctggta                              >  1:310102‑M1/1‑34 (MQ=255)                                                                                                                                                                                                                                ccataaataaaAGGCATGCCTAGAACTAAggaaggtgagaacaagtccctgatatgagagcatgtttttcatctggagccatagaacagggtccatcatgagtcagcaacttacctccgccgacagttaattcagcagtaagcgccgtcagaccagaaaagagatttgctggtcccgcatggatcagattctgctctggcaaaacatggtggaagtcatcgagccgttttaccccaaggcttcttatggca                        <  2:151442‑M1/247‑223 (MQ=255)                                                                                                                                                                                                                                 cAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccg                       <  1:465212‑M1/251‑224 (MQ=255)                                                                                                                                                                                                                                   tccccgcttattcgcaccttccCTAACGGCATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATACTCTTGCGTTTCATAAATAGTCCTTTTTATTGtt                      >  1:425375‑M2/23‑250 (MQ=255)                                                                                                                                                                                                                                         aaaGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgccctt               >  1:170535‑M1/1‑20 (MQ=255)                                                                                                                                                                                                                                                 gCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctg       >  1:357254‑M1/1‑12 (MQ=255)                                                                                                                                                                                                                                                    gTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctgga     >  1:33486‑M1/1‑9 (MQ=255)                                                                                                                                                                                                                                                     tGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaa    >  1:325205‑M1/1‑8 (MQ=255)                                                                                                                                                                                                                                                      gAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggctcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaac  >  1:337429‑M1/1‑7 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        TAAGCCAATCACAAGGCCAATGCCAACAAAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCTTTTTATTGTTACAGAGGGATTTATTCGTCT  >  NC_000913/2174111‑2174606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.