Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,778	2 bp→GG	intergenic (+231/+76)	nadR → / ← ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,778	0	A	G	100.0%	101.9 / NA	32	intergenic (+231/+77)	nadR/ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/14);  total (18/14)
*	NC_000913	4,628,779	0	C	G	100.0%	102.6 / NA	32	intergenic (+232/+76)	nadR/ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/14);  total (18/14)

AACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGC‑TGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGAT  >  NC_000913/4628557‑4629004                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                   aacggCTCAACGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGCTTCACAACGTATTGAAAtttcttggt                                                                                                                                                                                                       >  2:52066/5‑244 (MQ=255)      cTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttggtttgt                                                                                                                                                                                                   >  2:69036/1‑239 (MQ=255)        cAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAgg                                                                                                                                                                                               >  1:204194/1‑251 (MQ=255)                aGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATa                                                                                                                                                                                        >  2:119861/1‑250 (MQ=255)                         aataagttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                                                >  1:61836/7‑250 (MQ=255)                               ttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                                                                                         >  1:25981/1‑251 (MQ=255)                                         aGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACACCGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAtt                                                                                                                                                               >  2:72598/1‑251 (MQ=255)                                          gTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTGTTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAttt                                                                                                                                                              >  1:46514/1‑251 (MQ=255)                                          gTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAGGGCGTTCACGCCGCATCCGGCAttt                                                                                                                                                              >  1:200834/1‑251 (MQ=255)                                                         ggaggagGCTGACGAGATAAACCAAGGTTTGAAAAAACCTCCACGGGTGCCCCGCTACGAAATTGCGGATACTAAGGCGTTTGTAGGCCGGAAAAGGCGTTCCCGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAGGGCGTTCACGCCGCATCCGGCATTTTACGCATTACtt                                                                                                                                                  <  2:200834/244‑1 (MQ=255)                                                               ggcgtcagagaATAAACAAGGTTTGAAAAACCCTGCACGGGGGACCCGCTACGAAATTGCGGAAACTAAGTCTGTGGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACg                                                                                                                                         <  2:121243/244‑1 (MQ=255)                                                                 cTGTCGCGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCATTTTACGCATTACTTCGCAATACGCt                                                                                                                                       >  1:213514/1‑251 (MQ=255)                                                                     cGAGATTAAACAAGGTTTAAAAAACCCTGCACGGGTGACCCGCTCCGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGt                                                                                                                                    <  1:72598/250‑1 (MQ=255)                                                                              aCAAGGTTTGAAAAAACCTACAGGGGTGACCCCCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACg                                                                                                                          <  2:46514/251‑1 (MQ=255)                                                                                                tGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTc                                                                                                        >  2:73289/1‑251 (MQ=255)                                                                                                 gCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCCGTGCGTCt                                                                                                       >  2:106752/1‑251 (MQ=255)                                                                                                   aCGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAACGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATACGGCCTTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCCGTGCGTCTgc                                                                                                     >  2:95064/1‑251 (MQ=255)                                                                                                                 ccaaaaTTGCGGATGTTAGGGCTGTGGAAGGCCGGAAAAGGCGTCCGCGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTTCCGCGGCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCGTGGTTT‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCTTCTGCTCCCATCGTGCGtt                                                                                       <  2:194884/248‑1 (MQ=255)                                                                                                                             atacTAAGGTTGTTGTAGGCGGAAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCtt                                                                           <  1:52066/248‑1 (MQ=255)                                                                                                                               acTAAGTCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTc                                                                          <  2:236371/249‑1 (MQ=255)                                                                                                                                  aaGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTAc                                                                      <  1:119861/251‑1 (MQ=255)                                                                                                                                     gCTGTTGTAGGCGGGAAAAGCCGTTCCCGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCTGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTca                                                                   <  2:25981/251‑2 (MQ=255)                                                                                                                                            taggccGGAAAAGGCGTTCACGCCGTATCCGGAATTCAGTGCTCACTGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGATTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGACCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGt                                                            >  2:17923/5‑251 (MQ=255)                                                                                                                                             aggccgaaaaaggcgtTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGAAGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATACACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGt                                                            <  1:5586/236‑1 (MQ=255)                                                                                                                                                      aaggcgtTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGCTTCACAACGTATTGAAATTTC‑TTGGTTTGTCGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCga                                                   >  1:163896/6‑250 (MQ=255)                                                                                                                                                                cgccgtATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTGTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACt                                        <  2:264393/251‑1 (MQ=255)                                                                                                                                                                         cGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTc                                >  1:4745/1‑250 (MQ=255)                                                                                                                                                                                    gCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTGCGAAGAACTCAACTTTACCTTCATCCTGGTagtc                    <  1:98574/251‑4 (MQ=255)                                                                                                                                                                                           gCCTGATGCGACGCTTACCGCGTATTATCAGGCCGGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAggat              <  2:210024/250‑4 (MQ=255)                                                                                                                                                                                                       gCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGAt  >  2:207907/1‑250 (MQ=255)                                                                                                                                                                                                                gTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTAcc                                   >  1:42728/1‑208 (MQ=255)                                                                                                                                                                                                                gTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGGTACCTTCGAAGAACTCAACTTTAcc                                   <  2:42728/208‑1 (MQ=255)                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                   AACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGC‑TGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGAT  >  NC_000913/4628557‑4629004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑